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The map label for this gene is colS [H]

Identifier: 78048920

GI number: 78048920

Start: 3852309

End: 3853643

Strand: Reverse

Name: colS [H]

Synonym: XCV3364

Alternate gene names: 78048920

Gene position: 3853643-3852309 (Counterclockwise)

Preceding gene: 78048921

Following gene: 78048909

Centisome position: 74.42

GC content: 64.19

Gene sequence:

>1335_bases
ATGCCTGAAAGTACGAGCGAGACGCGGCCGGCGCGCAGGCGCGGCCGCTATCGGCGGCGCCTGCGCAGCCGCATCATTCT
GTCGTTCGTGTTGTTGGGCTTCTGCCTGACAACGCTGTTCGCCTTCGCCACCAACTGGGCGCGCTCGCGCGTGGAAAACC
AGTTGGTGGAAGACGTGATGAACCGCAACATCGACGCCTTCGCGCAACGCTTTTACTCCGATCCGATGCGCAATCCCGAT
CTGCCCCTGCAGCAGATGCGCGGGCGCGTGGTCAAAAGCGACAAGTTCGAAGCGCTGCGCCGCGAACAACCGGAATGGTA
CCAGCTGCCCGACGGCATCCACACCATCACCGGCGTCGACGAAAGCGGCAATACCTATTCGTACAAGCTGGCGGTGCGCA
AAACGCCGAGCGAATGGTTCTTCCTGGCCTACGACATGACCCAGGCGTTGAAGGGCGAAATCCAGCTCAAGCGCACCCTG
ATCTTGTCGGTATTGGTGTTCAGCGGGTTTTCGCTGCTGATCGGCTGGTGGTCTGCGTCCAAGGTGATGCGCCCGGTCTC
CGATCTGGCTGCGCGCCTGCGGGCCTATCGTGGCGGCACCAGCCAACCCAAACCGCTGGCGGCGCATTTTCCGGACGACG
AAGTCGGCCAGCTGGCCGAAGCGCTGGACGACTACTCGGCGCGTCTGACCGAAGTGGTGCAGCGCGACCGCGAATTCAAC
GCCGACGTCAGTCACGAACTGCGCACGCCGCTGGCGGTGATCCGCGGCGCTACCGAGCTGCTGCTGACCAAACCGAACCT
GGACGAAAAGGTGTTGCAGCGGCTGCAACGCATCCAGCGCGCCGAGCAGCAATGCAGCGATTTGATCGGCTCGTTGCTGT
TGCTCTCGCGCAACGAGCGCGGGCAGGGCAGCAGCAATGTCGCCAAGGTGGCCGAACAACTGATCGACTCGCACCGCGCG
CAGCTCGGTGGAAAACCTCTGGAGCTCGTGCTGGAAGGCGTGCGTGACCTGGTCATCGATGCCCCGGAATCGGCCCTGTC
GGTCGCGCTCGGCAACCTGATCGGCAACGCCGTCAAATACACCCAGGATGGCCAGGTGCGCGTGCGCGTGCTGGGCGATG
CAGTGGAAGTGATCGATTCGGGCCCCGGCCTGAGCGAAGAAGACGCCGCCAAACTGTTCCAGCGCGGCTACCGCGGGACC
CACGCCGGCCACTCGCAAGGCGGCGGCATCGGCTTGTCGATCGTCAGTCGTCTTTGTGATCTGTACGGCTGGCGGGTCAG
CGTGCGCCCAGGCCAGCAGCGCGGCGTCATCGCAACATTGGCCTTCCATCGCTGA

Upstream 100 bases:

>100_bases
CGCGTGCATATCCACGGCCTGCGCGCCGTGGTCGACAAGCCGTTCGATGTGCCGATGATCCAGACCCGCCACGGCATCGG
CTACCGCATCGCCTCGCCCG

Downstream 100 bases:

>100_bases
CCACCACACGCACTCCCGTAGGAGCGCACCCGGGCGCGACGGCCTTACCCATTGCGCCCCCAGGGCAGCGCCCCCAGACA
ACAACCACACCCAAAACCCA

Product: two-component system sensor protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 444; Mature: 443

Protein sequence:

>444_residues
MPESTSETRPARRRGRYRRRLRSRIILSFVLLGFCLTTLFAFATNWARSRVENQLVEDVMNRNIDAFAQRFYSDPMRNPD
LPLQQMRGRVVKSDKFEALRREQPEWYQLPDGIHTITGVDESGNTYSYKLAVRKTPSEWFFLAYDMTQALKGEIQLKRTL
ILSVLVFSGFSLLIGWWSASKVMRPVSDLAARLRAYRGGTSQPKPLAAHFPDDEVGQLAEALDDYSARLTEVVQRDREFN
ADVSHELRTPLAVIRGATELLLTKPNLDEKVLQRLQRIQRAEQQCSDLIGSLLLLSRNERGQGSSNVAKVAEQLIDSHRA
QLGGKPLELVLEGVRDLVIDAPESALSVALGNLIGNAVKYTQDGQVRVRVLGDAVEVIDSGPGLSEEDAAKLFQRGYRGT
HAGHSQGGGIGLSIVSRLCDLYGWRVSVRPGQQRGVIATLAFHR

Sequences:

>Translated_444_residues
MPESTSETRPARRRGRYRRRLRSRIILSFVLLGFCLTTLFAFATNWARSRVENQLVEDVMNRNIDAFAQRFYSDPMRNPD
LPLQQMRGRVVKSDKFEALRREQPEWYQLPDGIHTITGVDESGNTYSYKLAVRKTPSEWFFLAYDMTQALKGEIQLKRTL
ILSVLVFSGFSLLIGWWSASKVMRPVSDLAARLRAYRGGTSQPKPLAAHFPDDEVGQLAEALDDYSARLTEVVQRDREFN
ADVSHELRTPLAVIRGATELLLTKPNLDEKVLQRLQRIQRAEQQCSDLIGSLLLLSRNERGQGSSNVAKVAEQLIDSHRA
QLGGKPLELVLEGVRDLVIDAPESALSVALGNLIGNAVKYTQDGQVRVRVLGDAVEVIDSGPGLSEEDAAKLFQRGYRGT
HAGHSQGGGIGLSIVSRLCDLYGWRVSVRPGQQRGVIATLAFHR
>Mature_443_residues
PESTSETRPARRRGRYRRRLRSRIILSFVLLGFCLTTLFAFATNWARSRVENQLVEDVMNRNIDAFAQRFYSDPMRNPDL
PLQQMRGRVVKSDKFEALRREQPEWYQLPDGIHTITGVDESGNTYSYKLAVRKTPSEWFFLAYDMTQALKGEIQLKRTLI
LSVLVFSGFSLLIGWWSASKVMRPVSDLAARLRAYRGGTSQPKPLAAHFPDDEVGQLAEALDDYSARLTEVVQRDREFNA
DVSHELRTPLAVIRGATELLLTKPNLDEKVLQRLQRIQRAEQQCSDLIGSLLLLSRNERGQGSSNVAKVAEQLIDSHRAQ
LGGKPLELVLEGVRDLVIDAPESALSVALGNLIGNAVKYTQDGQVRVRVLGDAVEVIDSGPGLSEEDAAKLFQRGYRGTH
AGHSQGGGIGLSIVSRLCDLYGWRVSVRPGQQRGVIATLAFHR

Specific function: Member of the two-component regulatory system BasS/BasR. Autophosphorylates and activates BasR by phosphorylation. Plays a role in the adaptation of the organism to the host environment, in particular to neutrophils, and therefore it plays a role in virul

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1790861, Length=304, Percent_Identity=27.9605263157895, Blast_Score=96, Evalue=5e-21,
Organism=Escherichia coli, GI1790551, Length=275, Percent_Identity=29.8181818181818, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1789403, Length=268, Percent_Identity=30.9701492537313, Blast_Score=84, Evalue=3e-17,
Organism=Escherichia coli, GI1788393, Length=227, Percent_Identity=27.7533039647577, Blast_Score=79, Evalue=8e-16,
Organism=Escherichia coli, GI1789149, Length=350, Percent_Identity=28, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1786783, Length=231, Percent_Identity=28.5714285714286, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI1786600, Length=204, Percent_Identity=31.3725490196078, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI48994928, Length=227, Percent_Identity=31.7180616740088, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1788713, Length=222, Percent_Identity=27.9279279279279, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1790346, Length=196, Percent_Identity=31.1224489795918, Blast_Score=69, Evalue=6e-13,
Organism=Escherichia coli, GI1788279, Length=219, Percent_Identity=25.5707762557078, Blast_Score=69, Evalue=8e-13,
Organism=Escherichia coli, GI145693157, Length=233, Percent_Identity=27.8969957081545, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 49710; Mature: 49579

Theoretical pI: Translated: 9.82; Mature: 9.82

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPESTSETRPARRRGRYRRRLRSRIILSFVLLGFCLTTLFAFATNWARSRVENQLVEDVM
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NRNIDAFAQRFYSDPMRNPDLPLQQMRGRVVKSDKFEALRREQPEWYQLPDGIHTITGVD
HCCHHHHHHHHHCCCCCCCCCCHHHHCCCHHCCHHHHHHHCCCCCCEECCCCCEEEECCC
ESGNTYSYKLAVRKTPSEWFFLAYDMTQALKGEIQLKRTLILSVLVFSGFSLLIGWWSAS
CCCCEEEEEEEEEECCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
KVMRPVSDLAARLRAYRGGTSQPKPLAAHFPDDEVGQLAEALDDYSARLTEVVQRDREFN
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
ADVSHELRTPLAVIRGATELLLTKPNLDEKVLQRLQRIQRAEQQCSDLIGSLLLLSRNER
CCHHHHHHHHHHHHHCCHHHEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
GQGSSNVAKVAEQLIDSHRAQLGGKPLELVLEGVRDLVIDAPESALSVALGNLIGNAVKY
CCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHC
TQDGQVRVRVLGDAVEVIDSGPGLSEEDAAKLFQRGYRGTHAGHSQGGGIGLSIVSRLCD
CCCCCEEEEEHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHH
LYGWRVSVRPGQQRGVIATLAFHR
HHCCEEEECCCCCCCEEEEECCCC
>Mature Secondary Structure 
PESTSETRPARRRGRYRRRLRSRIILSFVLLGFCLTTLFAFATNWARSRVENQLVEDVM
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NRNIDAFAQRFYSDPMRNPDLPLQQMRGRVVKSDKFEALRREQPEWYQLPDGIHTITGVD
HCCHHHHHHHHHCCCCCCCCCCHHHHCCCHHCCHHHHHHHCCCCCCEECCCCCEEEECCC
ESGNTYSYKLAVRKTPSEWFFLAYDMTQALKGEIQLKRTLILSVLVFSGFSLLIGWWSAS
CCCCEEEEEEEEEECCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
KVMRPVSDLAARLRAYRGGTSQPKPLAAHFPDDEVGQLAEALDDYSARLTEVVQRDREFN
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
ADVSHELRTPLAVIRGATELLLTKPNLDEKVLQRLQRIQRAEQQCSDLIGSLLLLSRNER
CCHHHHHHHHHHHHHCCHHHEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
GQGSSNVAKVAEQLIDSHRAQLGGKPLELVLEGVRDLVIDAPESALSVALGNLIGNAVKY
CCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHC
TQDGQVRVRVLGDAVEVIDSGPGLSEEDAAKLFQRGYRGTHAGHSQGGGIGLSIVSRLCD
CCCCCEEEEEHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHH
LYGWRVSVRPGQQRGVIATLAFHR
HHCCEEEECCCCCCCEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8391535; 11677609 [H]