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The map label for this gene is pilB [H]

Identifier: 78048908

GI number: 78048908

Start: 3838617

End: 3840350

Strand: Reverse

Name: pilB [H]

Synonym: XCV3352

Alternate gene names: 78048908

Gene position: 3840350-3838617 (Counterclockwise)

Preceding gene: 78048909

Following gene: 78048905

Centisome position: 74.16

GC content: 61.82

Gene sequence:

>1734_bases
ATGAGTGCAGTCGCGGCGAACTTAGTGGGAATTACAGGCATTGCACGTCGTTTGGTGCAGGATGGAGCACTGGATGAGGC
AAGTGCCCGTACCGCAATGGACCAAGCCGCGGCGGCGAAGGTTCCGCTTCCCCAATGGTTCTCCGAGAAAAAGCTGGTTT
CAGCGTCTCAATTGGCCGCTGCCAACGCAGTCGAGTTCGGCATGCCGCTGCTCGATGTTTCTGCATTCGACGCCAGCCAG
AACGCAGTAAAGCTCGTCAGTGAGGAGCTGCTCCAGAAGCACCAGGTGCTGCCGCTGTTCAAGCGCGGCAACCGGTTGTT
CGTGGGGGTGAGCAATCCGACCCAGACCCGGGCGCTGGACGACATCAAGTTCCATACGAACTTGGTGGTCGAGGCCATTC
TTGTCGACGAGGATCAGATCCGCCGGACGCTCGAACAGTGGCAGGCCAGCAACGCGTCGTTGGGTTCGTCGCTTGGCAAC
GACGACGAGGAAATGGGGGATCTGGACGTCTCGGCCGGCGACGAGGACATGGGCGCCGGCGGGGATTCCGGGGTCGATGC
CAAGGGCGACGACACGCCGGTGGTGAAGTTCGTCAACAAGGTGCTGGTGGATGCGATCCGGCGGGGAGCCTCGGACATCC
ATTTCGAGCCGTATGAAGACGACTACCGGGTGCGCTTGCGCATCGATGGCCTGTTGAAGAACGTGGCCAAGGCGCCGGTG
AAGCTGAACCAGCGCATCGCAGCGCGGTTGAAGGTGATGTCGCAGCTGGATATCGCCGAGAAGCGGGTGCCGCAGGACGG
GCGCATCAAGCTCAACCTGTCCAAGACCAAGCAGATCGACTTCCGTGTCAGCACCTTGCCGACCCTGTTCGGAGAGAAGG
TGGTGCTGCGTATCCTGGACGGCAGCGCGGCCAAGCTGGGCATCGACAAGCTGGGCTACGAGCCGGACCAGCAGAAGCTG
TTCCTGGAAGCGATCCACAAGCCCTATGGCATGGTGCTGGTGACCGGGCCGACCGGCTCGGGCAAGACGGTGTCGTTGTA
CACGGCGCTGGGCATCCTCAACGACGAGACACGCAATATCTCCACCGCGGAGGACCCGGTCGAAATCCGCCTGCCTGGCG
TCAATCAGGTACAGCAGAACAACAAGCGTGGCATGACCTTTGCAGCAGCGCTGCGCTCGTTCCTGCGTCAGGACCCGGAC
ATCATCATGGTCGGCGAAATCCGTGACCTGGAGACGGCCGAGATTGCGATCAAGGCGGCGCAGACCGGTCACATGGTGTT
GTCGACGTTGCACACCAACGATGCGCCGCAGACCATCGCACGCTTGATGAACATGGGCATCGCGCCCTACAACATTACCT
CGTCGGTGACCTTGGTGATCGCGCAACGTCTGGCGCGGCGGTTGTGCAACAACTGCAAGCGCAAGTCGACGCTGCCTGAC
AACGCCTTGCTGGCCGAAGGATTCACCCCCGCACAGCTTGCTGCCGGGATCGAGCTGTATGAGGCGGTCGGTTGCGACGA
GTGCACCGAAGGCTACAAGGGGCGTACCGGTATCTACCAGGTCATGCCGATGACCGACGAGATCGGCGCGATCGTGCTGG
AGGGCGGCAATGCGATGCAGATTGCCGAGGCCGCGCAGGCGATCGGTATCCGCGATTTGCGGCAGTCGGCCTTGATGAAG
GCTGCGCAGGGGGTGACCAGCCTGGCCGAGATCAATCGGGTGACCAAGGACTAA

Upstream 100 bases:

>100_bases
ATTTGCCAAGCCCGCAGATGCGGGCTTGGTTTTTTGTCTTTCTGTTGGCGAAAAGATAGGAAGTAGTAAAGTACTGTTAC
TCTAAATTGGGAAGATTGGG

Downstream 100 bases:

>100_bases
GGGCTGGGAATCGGGATTGGGGAATCGAAAAGCCTGGGAACTCTACGTCCCAGGCCTTTTTGTTTGGACAGCTGGGCCAA
GGATTGCAGCGGGCCACCGC

Product: Tfp pilus assembly pathway ATPase PilB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 577; Mature: 576

Protein sequence:

>577_residues
MSAVAANLVGITGIARRLVQDGALDEASARTAMDQAAAAKVPLPQWFSEKKLVSASQLAAANAVEFGMPLLDVSAFDASQ
NAVKLVSEELLQKHQVLPLFKRGNRLFVGVSNPTQTRALDDIKFHTNLVVEAILVDEDQIRRTLEQWQASNASLGSSLGN
DDEEMGDLDVSAGDEDMGAGGDSGVDAKGDDTPVVKFVNKVLVDAIRRGASDIHFEPYEDDYRVRLRIDGLLKNVAKAPV
KLNQRIAARLKVMSQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDGSAAKLGIDKLGYEPDQQKL
FLEAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDPD
IIMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNNCKRKSTLPD
NALLAEGFTPAQLAAGIELYEAVGCDECTEGYKGRTGIYQVMPMTDEIGAIVLEGGNAMQIAEAAQAIGIRDLRQSALMK
AAQGVTSLAEINRVTKD

Sequences:

>Translated_577_residues
MSAVAANLVGITGIARRLVQDGALDEASARTAMDQAAAAKVPLPQWFSEKKLVSASQLAAANAVEFGMPLLDVSAFDASQ
NAVKLVSEELLQKHQVLPLFKRGNRLFVGVSNPTQTRALDDIKFHTNLVVEAILVDEDQIRRTLEQWQASNASLGSSLGN
DDEEMGDLDVSAGDEDMGAGGDSGVDAKGDDTPVVKFVNKVLVDAIRRGASDIHFEPYEDDYRVRLRIDGLLKNVAKAPV
KLNQRIAARLKVMSQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDGSAAKLGIDKLGYEPDQQKL
FLEAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDPD
IIMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNNCKRKSTLPD
NALLAEGFTPAQLAAGIELYEAVGCDECTEGYKGRTGIYQVMPMTDEIGAIVLEGGNAMQIAEAAQAIGIRDLRQSALMK
AAQGVTSLAEINRVTKD
>Mature_576_residues
SAVAANLVGITGIARRLVQDGALDEASARTAMDQAAAAKVPLPQWFSEKKLVSASQLAAANAVEFGMPLLDVSAFDASQN
AVKLVSEELLQKHQVLPLFKRGNRLFVGVSNPTQTRALDDIKFHTNLVVEAILVDEDQIRRTLEQWQASNASLGSSLGND
DEEMGDLDVSAGDEDMGAGGDSGVDAKGDDTPVVKFVNKVLVDAIRRGASDIHFEPYEDDYRVRLRIDGLLKNVAKAPVK
LNQRIAARLKVMSQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDGSAAKLGIDKLGYEPDQQKLF
LEAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDPDI
IMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNNCKRKSTLPDN
ALLAEGFTPAQLAAGIELYEAVGCDECTEGYKGRTGIYQVMPMTDEIGAIVLEGGNAMQIAEAAQAIGIRDLRQSALMKA
AQGVTSLAEINRVTKD

Specific function: Involved in the translocation of the type IV pilin (pilA) [H]

COG id: COG2804

COG function: function code NU; Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1786296, Length=386, Percent_Identity=45.3367875647668, Blast_Score=339, Evalue=2e-94,
Organism=Escherichia coli, GI1789723, Length=383, Percent_Identity=45.1697127937337, Blast_Score=327, Evalue=1e-90,
Organism=Escherichia coli, GI87082188, Length=184, Percent_Identity=37.5, Blast_Score=112, Evalue=7e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013374
- InterPro:   IPR007831
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E; PF05157 GSPII_E_N [H]

EC number: NA

Molecular weight: Translated: 62411; Mature: 62280

Theoretical pI: Translated: 5.45; Mature: 5.45

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAVAANLVGITGIARRLVQDGALDEASARTAMDQAAAAKVPLPQWFSEKKLVSASQLAA
CCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCHHHHCHHHHHHHHHHHH
ANAVEFGMPLLDVSAFDASQNAVKLVSEELLQKHQVLPLFKRGNRLFVGVSNPTQTRALD
HHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCHHHHCCCEEEEEECCCCHHHHHH
DIKFHTNLVVEAILVDEDQIRRTLEQWQASNASLGSSLGNDDEEMGDLDVSAGDEDMGAG
HHHHHHHHEEEEEECCHHHHHHHHHHHHCCCCHHCCCCCCCCHHHCCCCCCCCCCCCCCC
GDSGVDAKGDDTPVVKFVNKVLVDAIRRGASDIHFEPYEDDYRVRLRIDGLLKNVAKAPV
CCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEHHHHHHHHHCCH
KLNQRIAARLKVMSQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILD
HHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCEEEEEHHHHHHHHCCHHEEEEEC
GSAAKLGIDKLGYEPDQQKLFLEAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNI
CCCHHCCHHHCCCCCCHHHHHHHHHCCCCCEEEEECCCCCCCEEEEEEEHHHCCCCCCCC
STAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDPDIIMVGEIRDLETAEIAIKAA
CCCCCCEEEECCCCHHHHHCCCCCCHHHHHHHHHHHCCCCEEEECCCCCCCHHHEEEEEC
QTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNNCKRKSTLPD
CCCCEEEEECCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCC
NALLAEGFTPAQLAAGIELYEAVGCDECTEGYKGRTGIYQVMPMTDEIGAIVLEGGNAMQ
CCCEECCCCHHHHHHHHHHHHHCCCHHHHCCCCCCCCEEEEECCCCCCCEEEECCCCCHH
IAEAAQAIGIRDLRQSALMKAAQGVTSLAEINRVTKD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SAVAANLVGITGIARRLVQDGALDEASARTAMDQAAAAKVPLPQWFSEKKLVSASQLAA
CCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCHHHHCHHHHHHHHHHHH
ANAVEFGMPLLDVSAFDASQNAVKLVSEELLQKHQVLPLFKRGNRLFVGVSNPTQTRALD
HHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCHHHHCCCEEEEEECCCCHHHHHH
DIKFHTNLVVEAILVDEDQIRRTLEQWQASNASLGSSLGNDDEEMGDLDVSAGDEDMGAG
HHHHHHHHEEEEEECCHHHHHHHHHHHHCCCCHHCCCCCCCCHHHCCCCCCCCCCCCCCC
GDSGVDAKGDDTPVVKFVNKVLVDAIRRGASDIHFEPYEDDYRVRLRIDGLLKNVAKAPV
CCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEHHHHHHHHHCCH
KLNQRIAARLKVMSQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILD
HHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCEEEEEHHHHHHHHCCHHEEEEEC
GSAAKLGIDKLGYEPDQQKLFLEAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNI
CCCHHCCHHHCCCCCCHHHHHHHHHCCCCCEEEEECCCCCCCEEEEEEEHHHCCCCCCCC
STAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDPDIIMVGEIRDLETAEIAIKAA
CCCCCCEEEECCCCHHHHHCCCCCCHHHHHHHHHHHCCCCEEEECCCCCCCHHHEEEEEC
QTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNNCKRKSTLPD
CCCCEEEEECCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCC
NALLAEGFTPAQLAAGIELYEAVGCDECTEGYKGRTGIYQVMPMTDEIGAIVLEGGNAMQ
CCCEECCCCHHHHHHHHHHHHHCCCHHHHCCCCCCCCEEEEECCCCCCCEEEECCCCCHH
IAEAAQAIGIRDLRQSALMKAAQGVTSLAEINRVTKD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1971619; 10984043 [H]