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The map label for this gene is rsuA1 [H]

Identifier: 78048815

GI number: 78048815

Start: 3727646

End: 3728356

Strand: Reverse

Name: rsuA1 [H]

Synonym: XCV3259

Alternate gene names: 78048815

Gene position: 3728356-3727646 (Counterclockwise)

Preceding gene: 78048818

Following gene: 78048809

Centisome position: 72.0

GC content: 65.82

Gene sequence:

>711_bases
ATGTCTGATCCGATCCGTCTCGATAAATGTATAGCTGCCGCGTTCGGCTGCTCGCGTGGCGAGGCCCAGCAGTACATCGA
GGGTGGGTGGGTCAGCGTGGACGGCGTGGTGGTCGAAGAACCGCAGACGCTGGCTACCGCGGCGCAGGTTACCCTGGCCG
AAAATGCGGTACTGGCGCCTGCCGAGCCCGCCACCTTGTTGCTGCACAAGCCGGCAGGCATCTGCGTCGATGCCGCGCTG
GCGCTGGCCACGCCGGCAACGCGCAGCGCACTGGATCAGGCGGACGTACGTGTGCTGAAACGGCACTTCCTACGCTTGCA
CGCACCGCTGCCGCTGGAAGACGCCGCAAGCGGCCTGCTGATCCTGAGCCAGGATGGCCGCGTGCTGCGCCGGTTGAGCG
CTGACGCCAGCGCGATCGAGCAGGAATTTCTGGTCGAAGTGCGGGGTGAATTGCGCCCTTACGGCATGGCGCGGCTGGCG
CATGGCTTGGTCTATCAGCACCGCAGTCTGTCGCCTTGCAAGGTGAGCTGGCAAAACGAAGACCGCCTGCGGTTTGCGAT
CAAGCACGTGCAGCCGGGCCAATTGCGCTACATGTGCGGCGAAGTGGGCCCGGAGGTCGTGAGCATTCGCCGCCTGCGCG
TGGGCCGGGTGTCGCTGGGCAAGCTGGCGATCGGTGAATGGCGCTACCTGCCGGCAGGTGAGCGGTTCTGA

Upstream 100 bases:

>100_bases
GTCCAATGCATTGGGCAACGCTCCATCGCTGCCGTGCAACGACTCACGCGGGCGAGCAGCCAGCACGTACACTGCCGTCC
CCTGATGCTGAGCTCGCCCG

Downstream 100 bases:

>100_bases
CTGCAGCACGGAGTATGCGGCTGCAGCCGCTGGAATCACGTCGCGCTGTGTGCAAGCGTGCTCAGTCACGGCCAGCAGCG
CATGCAGCGATTATTTCTTG

Product: ribosomal small subunit pseudouridylate synthase

Products: pseudouridine 5'-phosphate; H2O [C]

Alternate protein names: 16S pseudouridine 516 synthase; 16S pseudouridylate 516 synthase; rRNA pseudouridylate synthase A; rRNA-uridine isomerase A [H]

Number of amino acids: Translated: 236; Mature: 235

Protein sequence:

>236_residues
MSDPIRLDKCIAAAFGCSRGEAQQYIEGGWVSVDGVVVEEPQTLATAAQVTLAENAVLAPAEPATLLLHKPAGICVDAAL
ALATPATRSALDQADVRVLKRHFLRLHAPLPLEDAASGLLILSQDGRVLRRLSADASAIEQEFLVEVRGELRPYGMARLA
HGLVYQHRSLSPCKVSWQNEDRLRFAIKHVQPGQLRYMCGEVGPEVVSIRRLRVGRVSLGKLAIGEWRYLPAGERF

Sequences:

>Translated_236_residues
MSDPIRLDKCIAAAFGCSRGEAQQYIEGGWVSVDGVVVEEPQTLATAAQVTLAENAVLAPAEPATLLLHKPAGICVDAAL
ALATPATRSALDQADVRVLKRHFLRLHAPLPLEDAASGLLILSQDGRVLRRLSADASAIEQEFLVEVRGELRPYGMARLA
HGLVYQHRSLSPCKVSWQNEDRLRFAIKHVQPGQLRYMCGEVGPEVVSIRRLRVGRVSLGKLAIGEWRYLPAGERF
>Mature_235_residues
SDPIRLDKCIAAAFGCSRGEAQQYIEGGWVSVDGVVVEEPQTLATAAQVTLAENAVLAPAEPATLLLHKPAGICVDAALA
LATPATRSALDQADVRVLKRHFLRLHAPLPLEDAASGLLILSQDGRVLRRLSADASAIEQEFLVEVRGELRPYGMARLAH
GLVYQHRSLSPCKVSWQNEDRLRFAIKHVQPGQLRYMCGEVGPEVVSIRRLRVGRVSLGKLAIGEWRYLPAGERF

Specific function: Responsible for synthesis of pseudouridine from uracil- 516 in 16S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788510, Length=235, Percent_Identity=27.6595744680851, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1790453, Length=236, Percent_Identity=25.8474576271186, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: 4.2.1.70 [C]

Molecular weight: Translated: 25760; Mature: 25629

Theoretical pI: Translated: 8.22; Mature: 8.22

Prosite motif: PS50889 S4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDPIRLDKCIAAAFGCSRGEAQQYIEGGWVSVDGVVVEEPQTLATAAQVTLAENAVLAP
CCCCHHHHHHHHHHHCCCCCHHHHHHCCCCEEECCEEEECCHHHHHHHHHHHHCCEEECC
AEPATLLLHKPAGICVDAALALATPATRSALDQADVRVLKRHFLRLHAPLPLEDAASGLL
CCCCEEEEECCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCEE
ILSQDGRVLRRLSADASAIEQEFLVEVRGELRPYGMARLAHGLVYQHRSLSPCKVSWQNE
EECCCCHHHHHHHCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCEEECCCC
DRLRFAIKHVQPGQLRYMCGEVGPEVVSIRRLRVGRVSLGKLAIGEWRYLPAGERF
CCEEEEEECCCCCHHHHHHHHCCHHHHHHHHHHCCCEEECCEEECCEEECCCCCCC
>Mature Secondary Structure 
SDPIRLDKCIAAAFGCSRGEAQQYIEGGWVSVDGVVVEEPQTLATAAQVTLAENAVLAP
CCCHHHHHHHHHHHCCCCCHHHHHHCCCCEEECCEEEECCHHHHHHHHHHHHCCEEECC
AEPATLLLHKPAGICVDAALALATPATRSALDQADVRVLKRHFLRLHAPLPLEDAASGLL
CCCCEEEEECCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCEE
ILSQDGRVLRRLSADASAIEQEFLVEVRGELRPYGMARLAHGLVYQHRSLSPCKVSWQNE
EECCCCHHHHHHHCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCEEECCCC
DRLRFAIKHVQPGQLRYMCGEVGPEVVSIRRLRVGRVSLGKLAIGEWRYLPAGERF
CCEEEEEECCCCCHHHHHHHHCCHHHHHHHHHHCCCEEECCEEECCEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose phosphate [C]

Specific reaction: uracil + D-ribose phosphate = pseudouridine 5'-phosphate + H2O [C]

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430 [H]