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The map label for this gene is oprN9 [H]

Identifier: 78048558

GI number: 78048558

Start: 3412546

End: 3414051

Strand: Reverse

Name: oprN9 [H]

Synonym: XCV3002

Alternate gene names: 78048558

Gene position: 3414051-3412546 (Counterclockwise)

Preceding gene: 78048559

Following gene: 78048557

Centisome position: 65.93

GC content: 66.14

Gene sequence:

>1506_bases
ATGATCACGATTCGCACGACACTCTGCGCGCTTGGCGTGGCCAGCGTGCTGTCCGGTTGCACGCTGATGCCGCGCTACAC
ACGGCCCGACGCACCGGTGCCAGCGCAATTTGCCGGCACCGACGTGTCGGCAGCCCCTCCGGCTGCCACTGCCGATACCG
CCGGCACCGCCGACGCCGCGTACATTGCCGATATCGGCTGGCGCCAGGTCTTCACCGACCCGGCGCTGCAGCAGGTGATT
GCGCTAGCCTTGGACAATAACCGCGACCTGCGCGTGGCCGCGCTCAATATCGAGGTGGCACGCGCGCAATATCGGGTGGA
CCGCGCCGCGCTGCTGCCCGCCATCGAGGGCACCGGCACCGCCAACAATGCGCGCACGCCCTCGGAGCTTGCCATTCCCG
GCCAGCCGCAGGTGTTCCGCACCTTCAGCGCCAATATCGGCGTCAGTGCCTATGAGCTGGACCTGTTCGGCCGCGTGCGC
AGCCTGAAGGAACAGGCGTTGCAGCAATTCCTGTCCACCGCCGAGGCACGCCGCAGTACGCATATCAGCCTGGTGGCAGA
AGTGGCCACCGCCTATCTGACCTTGGCGGCCGACCAGCAGTTGCTGCAGCTGGCGCAATCCACCCTGGCCAGCCAGAGCG
ACAGTTATCGCCTGCAGCAGCGCAGCTTCGAACTGGGTGCGGCCTCGCAACTGACCTTGCGTCAGGCGCAGACCACGGTG
GAAACCGCACGCGTGGATGTGGAGCGCTACATCGCGCAGGTGGCGCAGGATCGCAATGCCCTGGTGCTGCTGGTCGGCAC
GCAAGTGCCGGTGGAATTGCTGCCGCATGCACTGCCCGACAACGCGAGCGTGGAAGGCAATGTGTTGGCCAGCGTGCCGG
CCGGCTTGCCGTCTCAGTTGCTGCAACGTCGCCCCGACATTCTGGAAGCCGAGCGCAACTTGCGTGCGGCCAACGCCAAT
ATCGGTGCGGCGCGGGCAGCGTTCTTCCCCAGCATCAGCCTGACCGCATCGACCGGTTCGTCCAGCAGTAGCCTGTCCAG
GCTATTCGATGCCGGGACGCGTGCCTGGAGCTTCGTGCCCACGCTGACCTTGCCGATCTTCAATGCCGGGCGCAATCGCG
CCAATCTGGACATGGCCAAGGCCAACCGCGATATCGAGGTGGCGCGCTACGAAAAGTCGATCCAAAGCGCGTTCCGCGAG
GTCTCCGACGCGTTGGCGCAGCGCGACACGCTGGGCAGGCAACTGCAGGCCCAGCAAGCGCTGGTGGATGCCACTGCCGA
TAGCTACCGATTGTCGCAGGCGCGTTTCGAACGCGGCATCGACAGCTATCTGCAGGCGCTGGATGCGCAGCGTTCGCTGT
ACAGCGCGCAGCAGACCTTGATCAACACCCAGCTGTCGCGCTTCACCAACCTGGTGACGTTCTACAAGGCCATGGGCGGG
GGCTGGCTGCAAAGCGCAGCGCCCACTGCCGCCGCGGCCCCGGCAACGGAGCCGCCGCGTGGATGA

Upstream 100 bases:

>100_bases
GCGATCTTCTTCGTGCCGCTGTTCTTCGTGCTGGTGTGCGGCCTGTTCCAGCGCCGTCCACAGCCTGCCTCGCCCGCACC
GCAACCGACAGGACACTGAC

Downstream 100 bases:

>100_bases
CGACGCACCGTCGGTTGCGCCCAGGCGTGCGCCGCACGACAAGCGTGGCGCCATCCTGGCGGCGGCACGCGTGCTGTTTC
AGCAGCACGGCTTCGACAGG

Product: outer membrane efflux protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 501; Mature: 501

Protein sequence:

>501_residues
MITIRTTLCALGVASVLSGCTLMPRYTRPDAPVPAQFAGTDVSAAPPAATADTAGTADAAYIADIGWRQVFTDPALQQVI
ALALDNNRDLRVAALNIEVARAQYRVDRAALLPAIEGTGTANNARTPSELAIPGQPQVFRTFSANIGVSAYELDLFGRVR
SLKEQALQQFLSTAEARRSTHISLVAEVATAYLTLAADQQLLQLAQSTLASQSDSYRLQQRSFELGAASQLTLRQAQTTV
ETARVDVERYIAQVAQDRNALVLLVGTQVPVELLPHALPDNASVEGNVLASVPAGLPSQLLQRRPDILEAERNLRAANAN
IGAARAAFFPSISLTASTGSSSSSLSRLFDAGTRAWSFVPTLTLPIFNAGRNRANLDMAKANRDIEVARYEKSIQSAFRE
VSDALAQRDTLGRQLQAQQALVDATADSYRLSQARFERGIDSYLQALDAQRSLYSAQQTLINTQLSRFTNLVTFYKAMGG
GWLQSAAPTAAAAPATEPPRG

Sequences:

>Translated_501_residues
MITIRTTLCALGVASVLSGCTLMPRYTRPDAPVPAQFAGTDVSAAPPAATADTAGTADAAYIADIGWRQVFTDPALQQVI
ALALDNNRDLRVAALNIEVARAQYRVDRAALLPAIEGTGTANNARTPSELAIPGQPQVFRTFSANIGVSAYELDLFGRVR
SLKEQALQQFLSTAEARRSTHISLVAEVATAYLTLAADQQLLQLAQSTLASQSDSYRLQQRSFELGAASQLTLRQAQTTV
ETARVDVERYIAQVAQDRNALVLLVGTQVPVELLPHALPDNASVEGNVLASVPAGLPSQLLQRRPDILEAERNLRAANAN
IGAARAAFFPSISLTASTGSSSSSLSRLFDAGTRAWSFVPTLTLPIFNAGRNRANLDMAKANRDIEVARYEKSIQSAFRE
VSDALAQRDTLGRQLQAQQALVDATADSYRLSQARFERGIDSYLQALDAQRSLYSAQQTLINTQLSRFTNLVTFYKAMGG
GWLQSAAPTAAAAPATEPPRG
>Mature_501_residues
MITIRTTLCALGVASVLSGCTLMPRYTRPDAPVPAQFAGTDVSAAPPAATADTAGTADAAYIADIGWRQVFTDPALQQVI
ALALDNNRDLRVAALNIEVARAQYRVDRAALLPAIEGTGTANNARTPSELAIPGQPQVFRTFSANIGVSAYELDLFGRVR
SLKEQALQQFLSTAEARRSTHISLVAEVATAYLTLAADQQLLQLAQSTLASQSDSYRLQQRSFELGAASQLTLRQAQTTV
ETARVDVERYIAQVAQDRNALVLLVGTQVPVELLPHALPDNASVEGNVLASVPAGLPSQLLQRRPDILEAERNLRAANAN
IGAARAAFFPSISLTASTGSSSSSLSRLFDAGTRAWSFVPTLTLPIFNAGRNRANLDMAKANRDIEVARYEKSIQSAFRE
VSDALAQRDTLGRQLQAQQALVDATADSYRLSQARFERGIDSYLQALDAQRSLYSAQQTLINTQLSRFTNLVTFYKAMGG
GWLQSAAPTAAAAPATEPPRG

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor. Note=Attached to the outer membrane by a lipid anchor and via the transmembrane beta-barrel. The membrane anchor is not necessary for antibiotic efflux. In one report (PubMed:10889211) the Cys-18- Gly mutant is reported

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI1786785, Length=482, Percent_Identity=41.49377593361, Blast_Score=342, Evalue=4e-95,
Organism=Escherichia coli, GI2367348, Length=498, Percent_Identity=27.1084337349398, Blast_Score=145, Evalue=9e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010131 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 53702; Mature: 53702

Theoretical pI: Translated: 8.17; Mature: 8.17

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITIRTTLCALGVASVLSGCTLMPRYTRPDAPVPAQFAGTDVSAAPPAATADTAGTADAA
CEEEHHHHHHHHHHHHHCCCEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHH
YIADIGWRQVFTDPALQQVIALALDNNRDLRVAALNIEVARAQYRVDRAALLPAIEGTGT
HHHHCCHHHHHCCHHHHHHHHHHHCCCCCEEEEEEEEEHHHHHHHHHHHHHCCCCCCCCC
ANNARTPSELAIPGQPQVFRTFSANIGVSAYELDLFGRVRSLKEQALQQFLSTAEARRST
CCCCCCCCCCCCCCCCCEEEEECCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HISLVAEVATAYLTLAADQQLLQLAQSTLASQSDSYRLQQRSFELGAASQLTLRQAQTTV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHH
ETARVDVERYIAQVAQDRNALVLLVGTQVPVELLPHALPDNASVEGNVLASVPAGLPSQL
HHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHCCCCCCCCCCCCCEEEECCCCCHHHH
LQRRPDILEAERNLRAANANIGAARAAFFPSISLTASTGSSSSSLSRLFDAGTRAWSFVP
HHCCCCHHHHHHCCHHCCCCCCHHHHHHCCCEEEEECCCCCHHHHHHHHHCCCCCEEEHH
TLTLPIFNAGRNRANLDMAKANRDIEVARYEKSIQSAFREVSDALAQRDTLGRQLQAQQA
HHEEEHCCCCCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LVDATADSYRLSQARFERGIDSYLQALDAQRSLYSAQQTLINTQLSRFTNLVTFYKAMGG
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GWLQSAAPTAAAAPATEPPRG
CHHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MITIRTTLCALGVASVLSGCTLMPRYTRPDAPVPAQFAGTDVSAAPPAATADTAGTADAA
CEEEHHHHHHHHHHHHHCCCEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHH
YIADIGWRQVFTDPALQQVIALALDNNRDLRVAALNIEVARAQYRVDRAALLPAIEGTGT
HHHHCCHHHHHCCHHHHHHHHHHHCCCCCEEEEEEEEEHHHHHHHHHHHHHCCCCCCCCC
ANNARTPSELAIPGQPQVFRTFSANIGVSAYELDLFGRVRSLKEQALQQFLSTAEARRST
CCCCCCCCCCCCCCCCCEEEEECCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HISLVAEVATAYLTLAADQQLLQLAQSTLASQSDSYRLQQRSFELGAASQLTLRQAQTTV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHH
ETARVDVERYIAQVAQDRNALVLLVGTQVPVELLPHALPDNASVEGNVLASVPAGLPSQL
HHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHCCCCCCCCCCCCCEEEECCCCCHHHH
LQRRPDILEAERNLRAANANIGAARAAFFPSISLTASTGSSSSSLSRLFDAGTRAWSFVP
HHCCCCHHHHHHCCHHCCCCCCHHHHHHCCCEEEEECCCCCHHHHHHHHHCCCCCEEEHH
TLTLPIFNAGRNRANLDMAKANRDIEVARYEKSIQSAFREVSDALAQRDTLGRQLQAQQA
HHEEEHCCCCCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LVDATADSYRLSQARFERGIDSYLQALDAQRSLYSAQQTLINTQLSRFTNLVTFYKAMGG
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GWLQSAAPTAAAAPATEPPRG
CHHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8226684; 10889211; 10984043; 7968531; 8540696; 9401051; 9603892; 10952562 [H]