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The map label for this gene is trmJ [H]

Identifier: 78048137

GI number: 78048137

Start: 2909434

End: 2910198

Strand: Reverse

Name: trmJ [H]

Synonym: XCV2581

Alternate gene names: 78048137

Gene position: 2910198-2909434 (Counterclockwise)

Preceding gene: 78048139

Following gene: 78048136

Centisome position: 56.2

GC content: 68.89

Gene sequence:

>765_bases
ATGTCCGTCAGCCAGCGCATCCGATTTGTTCTTGTGGGTACCCAGCATCCAGGCAACATCGGCGCTGCAGCGCGTGCGAT
GAAGACCATGGGGGTTTCGCGCCTGGTGCTGGTGGCGCCGGAGCGCGCACTCGACGAAGACGCCTATCGGCGCTCGGCCG
GCGCAGAAGACGTGCTGAGCCAGGCGCCGATCTTCGCGACACTGGGCGAGGCGGTGGCCGACTGCACGCTGGTGATTGGC
TGCACTGCGCGCGCGCGCCGGGTGGCGCTGGAAGAACTGCTGCCGGACGAGGGCGCGCAGCGTGCCCTGGCCAAGGCGGG
CGAGCCGGCCGAGGTGGCCTTCGTATTCGGCCGCGAGCGTACCGGTCTGACCAACGACGAGCTGCAGCTGTGCCACGCCG
CGGTCCACATTCCCTCCGATCCGCAGTTCAGTTCGCTCAATCTGGCCGCGGCCGTGCAGGTGCTGGCGTACGAAGTGCGA
CTGGCGCAGCTGGCGGCAGGCCAGGCCGAACGCGCGCCGGCAGCGGCTCCGGGCTTGCGTGACGGGCCGGCCAGCCACGC
ACAGCTGGAAGGGATGTTCGGCCAGCTGGGCGACACGCTGGACGAGATCGACTTCCACAAGGGCCGTGCGCCGGAGTCGG
CGATGCGCAAGTTGCGCCGGTTGTTGCTGCGCGCAGAGATGACCGAGCAGGAGGTGCGGCTGATTCGCGGCATCCTGTCC
GATGCGCAGCGCATGGCCATGCTTGCCAAGCAGGCCGGAAACTGA

Upstream 100 bases:

>100_bases
AGGCAGAATCGGACAATGGCGAAGGTAAAAGAGCGGTCCGGCGCGAAAGCCGGCCGCGCAGTTTACCATCTACGGCCGAA
CAGCTTCCCGATCTCCGTTC

Downstream 100 bases:

>100_bases
CGTCTGTCACATCTTCAAAGATTCGGCTAGGCTGTCCGCGGCTCTAGGGGAGTGTCCGTTTTGCGCGGTCTACGATGGTG
TCTGATTGCCTGCTGGCTGA

Product: tRNA/rRNA methyltransferase

Products: NA

Alternate protein names: tRNA Cm32/Um32 methyltransferase [H]

Number of amino acids: Translated: 254; Mature: 253

Protein sequence:

>254_residues
MSVSQRIRFVLVGTQHPGNIGAAARAMKTMGVSRLVLVAPERALDEDAYRRSAGAEDVLSQAPIFATLGEAVADCTLVIG
CTARARRVALEELLPDEGAQRALAKAGEPAEVAFVFGRERTGLTNDELQLCHAAVHIPSDPQFSSLNLAAAVQVLAYEVR
LAQLAAGQAERAPAAAPGLRDGPASHAQLEGMFGQLGDTLDEIDFHKGRAPESAMRKLRRLLLRAEMTEQEVRLIRGILS
DAQRMAMLAKQAGN

Sequences:

>Translated_254_residues
MSVSQRIRFVLVGTQHPGNIGAAARAMKTMGVSRLVLVAPERALDEDAYRRSAGAEDVLSQAPIFATLGEAVADCTLVIG
CTARARRVALEELLPDEGAQRALAKAGEPAEVAFVFGRERTGLTNDELQLCHAAVHIPSDPQFSSLNLAAAVQVLAYEVR
LAQLAAGQAERAPAAAPGLRDGPASHAQLEGMFGQLGDTLDEIDFHKGRAPESAMRKLRRLLLRAEMTEQEVRLIRGILS
DAQRMAMLAKQAGN
>Mature_253_residues
SVSQRIRFVLVGTQHPGNIGAAARAMKTMGVSRLVLVAPERALDEDAYRRSAGAEDVLSQAPIFATLGEAVADCTLVIGC
TARARRVALEELLPDEGAQRALAKAGEPAEVAFVFGRERTGLTNDELQLCHAAVHIPSDPQFSSLNLAAAVQVLAYEVRL
AQLAAGQAERAPAAAPGLRDGPASHAQLEGMFGQLGDTLDEIDFHKGRAPESAMRKLRRLLLRAEMTEQEVRLIRGILSD
AQRMAMLAKQAGN

Specific function: Catalyzes the formation of 2'O-methylated cytidine (Cm32) or 2'O-methylated uridine (Um32) at position 32 in tRNA [H]

COG id: COG0565

COG function: function code J; rRNA methylase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1788881, Length=244, Percent_Identity=46.3114754098361, Blast_Score=191, Evalue=3e-50,
Organism=Escherichia coli, GI1790865, Length=154, Percent_Identity=37.012987012987, Blast_Score=94, Evalue=9e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004384
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 27155; Mature: 27023

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVSQRIRFVLVGTQHPGNIGAAARAMKTMGVSRLVLVAPERALDEDAYRRSAGAEDVLS
CCHHHEEEEEEEECCCCCCHHHHHHHHHHHCHHEEEEECCHHHHHHHHHHHCCCHHHHHH
QAPIFATLGEAVADCTLVIGCTARARRVALEELLPDEGAQRALAKAGEPAEVAFVFGRER
CCCHHHHHHHHHHHHHHEEECCHHHHHHHHHHHCCCCHHHHHHHHCCCCCEEEEEECCCC
TGLTNDELQLCHAAVHIPSDPQFSSLNLAAAVQVLAYEVRLAQLAAGQAERAPAAAPGLR
CCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
DGPASHAQLEGMFGQLGDTLDEIDFHKGRAPESAMRKLRRLLLRAEMTEQEVRLIRGILS
CCCCCHHHHHHHHHHHCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DAQRMAMLAKQAGN
HHHHHHHHHHHCCC
>Mature Secondary Structure 
SVSQRIRFVLVGTQHPGNIGAAARAMKTMGVSRLVLVAPERALDEDAYRRSAGAEDVLS
CHHHEEEEEEEECCCCCCHHHHHHHHHHHCHHEEEEECCHHHHHHHHHHHCCCHHHHHH
QAPIFATLGEAVADCTLVIGCTARARRVALEELLPDEGAQRALAKAGEPAEVAFVFGRER
CCCHHHHHHHHHHHHHHEEECCHHHHHHHHHHHCCCCHHHHHHHHCCCCCEEEEEECCCC
TGLTNDELQLCHAAVHIPSDPQFSSLNLAAAVQVLAYEVRLAQLAAGQAERAPAAAPGLR
CCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
DGPASHAQLEGMFGQLGDTLDEIDFHKGRAPESAMRKLRRLLLRAEMTEQEVRLIRGILS
CCCCCHHHHHHHHHHHCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DAQRMAMLAKQAGN
HHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA