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The map label for this gene is hcaE [H]

Identifier: 78047563

GI number: 78047563

Start: 2289563

End: 2290702

Strand: Reverse

Name: hcaE [H]

Synonym: XCV2007

Alternate gene names: 78047563

Gene position: 2290702-2289563 (Counterclockwise)

Preceding gene: 78047564

Following gene: 78047562

Centisome position: 44.24

GC content: 58.25

Gene sequence:

>1140_bases
ATGAGACGCCACGCACTTGACGTCAAACACTACACCAGCCCGCATACCTTCGAGCTGGAGCAGGCGCGGCTGTTCAGCCG
ATTGTGGACATTTGTCGGTTTTTCATCATTAGTGGCCAAACGTAATCAGTTCTTCGCGCGCCAGGTTGCTGGTGTGCCAG
TGCTGGTGCAGCGCACCGATGCCGGAATTCGCGCCTTTATCAATCAGTGCCCGCATCGTCAATCGGCGATCCAGATCGAC
AGCCATGGCAAGCGGCCGTTGGTGTGCCCGTATCACGCATGGTCGTTCGGACCGGAGGGCGAGTTGCGTGGCCTGCCCAA
CCCAGGTCTGTACCAATTCACCGCCGAAGAGAAGGACGCCATCTGTCTGCGCAAACTGCACCTAGAGCAGGTCGGCCAGC
TGTTGTTCGTCAATCTCTCCGAACAACCCTTGCCGCTGACCGAACAGTTTTCACCCGAGTACCTGGAGCAGCTGCGCCTG
GCGTCCTCGCATCTGGACTCGCAGATCATCTACAGCTGCCATCGGGTGCGCTACAACTGGAAGTTGAACATGGAAAACGT
CAAGGACTATAACCACGTCCCTTTCGTTCACCCCAAGACCTTCGGCCCGTTGATGACGAGTACGGACAAGGCGGCACGCA
CCCAGGAGTCTGGGTCCGACGCTGCTTCGCCGGTGAAACAACTACTACAGTCCAAGGCCGTTCCCGCATTGAGCGAGCTC
AGCTTCCCGGTCAAGGCGCATATCACAGCGCAAAGTAGCTGGTTTCGTTCGCTATGCGACCGCTACGGCGAGGATGAGGC
CTACTACAACTGGTTCGTCTACCCTAACGTCAACTTCTGCAGTGTGCGCGGCGACTATTTCCTGCTTCAGCAATACGATC
CGGTTTCGTCATCGGAAACCGACTACCACCTGTGGGTAATGACAGCGCAGCGCAAGCACGAGCGCACCGACTTCACGGCC
CTGTTGAGCGATCTGATCCGCGGCGAGCGTACCGTCATCGCGGAGGATACTGTCTTGTTGGAACGCATGCAGGCCGGTTT
CGGACCGCACTCCGACCACATCACGCACGGCGATTACGAGATTCAACTGGTGCGCCAGCATCGTTGGTACCGCGGCGCGG
TGCTGGAGGAAACGCATTGA

Upstream 100 bases:

>100_bases
GGTGGTAAAAGATGTTCCCTCCAACAGCACCGTGGCTGGCAATCCTGCGCGCGTCATCTTCAGTAGGTAGCGCGCAAATA
TGCACTTCAGGAGGTAGGAC

Downstream 100 bases:

>100_bases
CCTCACTCATCCTGCTGGGTCAGGACGATGCGCTAACGCAGGCGGTACGCGCGGCAAACGCATGCGGATTGGTGCACACC
GTGATCGAACTGAAATCGGC

Product: putative Rieske 2Fe-2S family protein

Products: NA

Alternate protein names: Digoxigenin subunit alpha [H]

Number of amino acids: Translated: 379; Mature: 379

Protein sequence:

>379_residues
MRRHALDVKHYTSPHTFELEQARLFSRLWTFVGFSSLVAKRNQFFARQVAGVPVLVQRTDAGIRAFINQCPHRQSAIQID
SHGKRPLVCPYHAWSFGPEGELRGLPNPGLYQFTAEEKDAICLRKLHLEQVGQLLFVNLSEQPLPLTEQFSPEYLEQLRL
ASSHLDSQIIYSCHRVRYNWKLNMENVKDYNHVPFVHPKTFGPLMTSTDKAARTQESGSDAASPVKQLLQSKAVPALSEL
SFPVKAHITAQSSWFRSLCDRYGEDEAYYNWFVYPNVNFCSVRGDYFLLQQYDPVSSSETDYHLWVMTAQRKHERTDFTA
LLSDLIRGERTVIAEDTVLLERMQAGFGPHSDHITHGDYEIQLVRQHRWYRGAVLEETH

Sequences:

>Translated_379_residues
MRRHALDVKHYTSPHTFELEQARLFSRLWTFVGFSSLVAKRNQFFARQVAGVPVLVQRTDAGIRAFINQCPHRQSAIQID
SHGKRPLVCPYHAWSFGPEGELRGLPNPGLYQFTAEEKDAICLRKLHLEQVGQLLFVNLSEQPLPLTEQFSPEYLEQLRL
ASSHLDSQIIYSCHRVRYNWKLNMENVKDYNHVPFVHPKTFGPLMTSTDKAARTQESGSDAASPVKQLLQSKAVPALSEL
SFPVKAHITAQSSWFRSLCDRYGEDEAYYNWFVYPNVNFCSVRGDYFLLQQYDPVSSSETDYHLWVMTAQRKHERTDFTA
LLSDLIRGERTVIAEDTVLLERMQAGFGPHSDHITHGDYEIQLVRQHRWYRGAVLEETH
>Mature_379_residues
MRRHALDVKHYTSPHTFELEQARLFSRLWTFVGFSSLVAKRNQFFARQVAGVPVLVQRTDAGIRAFINQCPHRQSAIQID
SHGKRPLVCPYHAWSFGPEGELRGLPNPGLYQFTAEEKDAICLRKLHLEQVGQLLFVNLSEQPLPLTEQFSPEYLEQLRL
ASSHLDSQIIYSCHRVRYNWKLNMENVKDYNHVPFVHPKTFGPLMTSTDKAARTQESGSDAASPVKQLLQSKAVPALSEL
SFPVKAHITAQSSWFRSLCDRYGEDEAYYNWFVYPNVNFCSVRGDYFLLQQYDPVSSSETDYHLWVMTAQRKHERTDFTA
LLSDLIRGERTVIAEDTVLLERMQAGFGPHSDHITHGDYEIQLVRQHRWYRGAVLEETH

Specific function: Part of the multicomponent 3-phenylpropionate dioxygenase. Converts 3-phenylpropionic acid (PP) and cinnamic acid (CI) into 3-phenylpropionate-dihydrodiol (PP-dihydrodiol) and cinnamic acid-dihydrodiol (CI-dihydrodiol), respectively [H]

COG id: COG4638

COG function: function code PR; Phenylpropionate dioxygenase and related ring-hydroxylating dioxygenases, large terminal subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Rieske domain [H]

Homologues:

Organism=Escherichia coli, GI1788888, Length=225, Percent_Identity=28, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1788103, Length=350, Percent_Identity=23.7142857142857, Blast_Score=80, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020875
- InterPro:   IPR017941
- InterPro:   IPR015881
- InterPro:   IPR015879
- InterPro:   IPR001663 [H]

Pfam domain/function: PF00355 Rieske; PF00848 Ring_hydroxyl_A [H]

EC number: =1.14.12.19 [H]

Molecular weight: Translated: 43753; Mature: 43753

Theoretical pI: Translated: 7.33; Mature: 7.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRHALDVKHYTSPHTFELEQARLFSRLWTFVGFSSLVAKRNQFFARQVAGVPVLVQRTD
CCCCCCCHHHCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECC
AGIRAFINQCPHRQSAIQIDSHGKRPLVCPYHAWSFGPEGELRGLPNPGLYQFTAEEKDA
HHHHHHHHHCCCCCCEEEECCCCCCCEECCCCCCCCCCCCCCCCCCCCCCEEEECCCCHH
ICLRKLHLEQVGQLLFVNLSEQPLPLTEQFSPEYLEQLRLASSHLDSQIIYSCHRVRYNW
HHHHHHHHHHHCCEEEEECCCCCCCCHHCCCHHHHHHHHHHHHHCCHHHHHHHHHEEEEE
KLNMENVKDYNHVPFVHPKTFGPLMTSTDKAARTQESGSDAASPVKQLLQSKAVPALSEL
EECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHCCCCCHHHC
SFPVKAHITAQSSWFRSLCDRYGEDEAYYNWFVYPNVNFCSVRGDYFLLQQYDPVSSSET
CCCEEEEEECHHHHHHHHHHHHCCCCCEEEEEEECCCEEEEECCCEEEEEECCCCCCCCC
DYHLWVMTAQRKHERTDFTALLSDLIRGERTVIAEDTVLLERMQAGFGPHSDHITHGDYE
CEEEEEEECCHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHCCCCCCCCCCCCCEE
IQLVRQHRWYRGAVLEETH
EEEEECCCCCCCCCCCCCC
>Mature Secondary Structure
MRRHALDVKHYTSPHTFELEQARLFSRLWTFVGFSSLVAKRNQFFARQVAGVPVLVQRTD
CCCCCCCHHHCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECC
AGIRAFINQCPHRQSAIQIDSHGKRPLVCPYHAWSFGPEGELRGLPNPGLYQFTAEEKDA
HHHHHHHHHCCCCCCEEEECCCCCCCEECCCCCCCCCCCCCCCCCCCCCCEEEECCCCHH
ICLRKLHLEQVGQLLFVNLSEQPLPLTEQFSPEYLEQLRLASSHLDSQIIYSCHRVRYNW
HHHHHHHHHHHCCEEEEECCCCCCCCHHCCCHHHHHHHHHHHHHCCHHHHHHHHHEEEEE
KLNMENVKDYNHVPFVHPKTFGPLMTSTDKAARTQESGSDAASPVKQLLQSKAVPALSEL
EECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHCCCCCHHHC
SFPVKAHITAQSSWFRSLCDRYGEDEAYYNWFVYPNVNFCSVRGDYFLLQQYDPVSSSET
CCCEEEEEECHHHHHHHHHHHHCCCCCEEEEEEECCCEEEEECCCEEEEEECCCCCCCCC
DYHLWVMTAQRKHERTDFTALLSDLIRGERTVIAEDTVLLERMQAGFGPHSDHITHGDYE
CEEEEEEECCHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHCCCCCCCCCCCCCEE
IQLVRQHRWYRGAVLEETH
EEEEECCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]