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The map label for this gene is rnhB [H]

Identifier: 78047020

GI number: 78047020

Start: 1646379

End: 1646768

Strand: Reverse

Name: rnhB [H]

Synonym: XCV1464

Alternate gene names: 78047020

Gene position: 1646768-1646379 (Counterclockwise)

Preceding gene: 78047021

Following gene: 78047019

Centisome position: 31.8

GC content: 69.74

Gene sequence:

>390_bases
ATGCGGCGCGCGGTGGAAGGCGTGGCGCATGTGGCCGGGTTCGCGCGCATCGATGGCAACCGCGTGCCCAAAGGCCTGCC
CTGCCCGGCCGAGGCATTGATCGGTGGCGACGCGCTGGATCGCGCCATCATGGCCGCCTCGATCGTGGCCAAGGTGACGC
GCGACCGGCTGATGCACGAACTGCACACGCGGCACCCGGAATATCGCTTCGATCAGCACAAGGGCTACAGCACGCCGGTG
CATCTGGCCGCGTTGCAAACCCACGGGCCATGCCCCCAGCATCGGCGCAGCTTTGCGCCGGTGCGCCTGGCGCTGGAAGG
CCGCAACCCGGGGCCCGGGACCCGGGACCCGGAACAGCTGCAAGTGGGCAGGTTGGTGGTGCCGCTTTAG

Upstream 100 bases:

>100_bases
CGCATCGTCGACCGGGCGCTGGCGTGGTCGGTGGTGTTGATCCACAGCGAAGAGATCGACCGCATCAATATCTACCAGGC
GACCATGCTCGGCATGCGGC

Downstream 100 bases:

>100_bases
AGGTCGGGACCCGGGACCCGGAAAAGCACCAGCACAGCAACAGCAAAGAAACGACGAGTGGCGTCCCGCCACGTCTTCCC
GCTTTGCTTTCCCGGCCCCC

Product: ribonuclease HII

Products: NA

Alternate protein names: RNase HII [H]

Number of amino acids: Translated: 129; Mature: 129

Protein sequence:

>129_residues
MRRAVEGVAHVAGFARIDGNRVPKGLPCPAEALIGGDALDRAIMAASIVAKVTRDRLMHELHTRHPEYRFDQHKGYSTPV
HLAALQTHGPCPQHRRSFAPVRLALEGRNPGPGTRDPEQLQVGRLVVPL

Sequences:

>Translated_129_residues
MRRAVEGVAHVAGFARIDGNRVPKGLPCPAEALIGGDALDRAIMAASIVAKVTRDRLMHELHTRHPEYRFDQHKGYSTPV
HLAALQTHGPCPQHRRSFAPVRLALEGRNPGPGTRDPEQLQVGRLVVPL
>Mature_129_residues
MRRAVEGVAHVAGFARIDGNRVPKGLPCPAEALIGGDALDRAIMAASIVAKVTRDRLMHELHTRHPEYRFDQHKGYSTPV
HLAALQTHGPCPQHRRSFAPVRLALEGRNPGPGTRDPEQLQVGRLVVPL

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids [H]

COG id: COG0164

COG function: function code L; Ribonuclease HII

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase HII family [H]

Homologues:

Organism=Escherichia coli, GI1786380, Length=105, Percent_Identity=52.3809523809524, Blast_Score=96, Evalue=7e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022898
- InterPro:   IPR001352
- InterPro:   IPR012337 [H]

Pfam domain/function: PF01351 RNase_HII [H]

EC number: =3.1.26.4 [H]

Molecular weight: Translated: 14129; Mature: 14129

Theoretical pI: Translated: 10.65; Mature: 10.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRAVEGVAHVAGFARIDGNRVPKGLPCPAEALIGGDALDRAIMAASIVAKVTRDRLMHE
CCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
LHTRHPEYRFDQHKGYSTPVHLAALQTHGPCPQHRRSFAPVRLALEGRNPGPGTRDPEQL
HHHCCCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHCCCCEEEEEECCCCCCCCCCHHHH
QVGRLVVPL
HHCEEECCC
>Mature Secondary Structure
MRRAVEGVAHVAGFARIDGNRVPKGLPCPAEALIGGDALDRAIMAASIVAKVTRDRLMHE
CCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
LHTRHPEYRFDQHKGYSTPVHLAALQTHGPCPQHRRSFAPVRLALEGRNPGPGTRDPEQL
HHHCCCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHCCCCEEEEEECCCCCCCCCCHHHH
QVGRLVVPL
HHCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12024217 [H]