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The map label for this gene is rnfB [H]

Identifier: 78046995

GI number: 78046995

Start: 1617552

End: 1618022

Strand: Reverse

Name: rnfB [H]

Synonym: XCV1439

Alternate gene names: 78046995

Gene position: 1618022-1617552 (Counterclockwise)

Preceding gene: 78046996

Following gene: 78046994

Centisome position: 31.25

GC content: 67.09

Gene sequence:

>471_bases
TTGCGCAACAACGCTGGTATCGCGGGCATCTGCTGCACTGAAATGGCCGCGACGTCCGCCCCTGCACCCGATCTGGTCGA
ACGCCTCGACCGCCTGCTGCCGCAGACCCAATGCGGGCAATGCGGGTATGACGGCTGTCGTCCGTACGCGCAGGCGATGG
CAGATGGCCAGGCGACGATCGATCACTGCCCGCCCGGCGGCGATGCCGGGGCACGCGCCTTGGCGCAGGTGCTGGGCGTG
CCTGCACGTCCCTACGATCGCAGTCGCGGCGCACACAAACTGCCGCAGGTGGCCTGGATCGTGGAAGCCGACTGCATCGG
CTGCACCAAGTGCATCCAGGCCTGCCCGGTGGATGCCATCGTCGGCGGCGCCAAGCACATGCACACGGTGATCGCACCGT
TGTGTACCGGTTGCGAACTGTGTCTGCCGGCGTGCCCGGTGGATTGCATCGAGTTGCATCCGACCGGCTGA

Upstream 100 bases:

>100_bases
CCGCACAAGGTCGCGCGGATTTGCAGTCAATACGACTTGCATCGGTACGACTGCGTGCACGCCTATGGCGACAGCCGCGA
AGACAAACCGATGCTCGCGC

Downstream 100 bases:

>100_bases
GGCTGCGGTCAGCGCGATACCGCGTCCAGTCGATGCCTGCATGTTCATGGATCGGCGCGCAGTCCAGCATGACCACCAGC
TGCGAAAGGCGGATTTCGAA

Product: ferredoxin

Products: NA

Alternate protein names: Nitrogen fixation protein rnfB [H]

Number of amino acids: Translated: 156; Mature: 156

Protein sequence:

>156_residues
MRNNAGIAGICCTEMAATSAPAPDLVERLDRLLPQTQCGQCGYDGCRPYAQAMADGQATIDHCPPGGDAGARALAQVLGV
PARPYDRSRGAHKLPQVAWIVEADCIGCTKCIQACPVDAIVGGAKHMHTVIAPLCTGCELCLPACPVDCIELHPTG

Sequences:

>Translated_156_residues
MRNNAGIAGICCTEMAATSAPAPDLVERLDRLLPQTQCGQCGYDGCRPYAQAMADGQATIDHCPPGGDAGARALAQVLGV
PARPYDRSRGAHKLPQVAWIVEADCIGCTKCIQACPVDAIVGGAKHMHTVIAPLCTGCELCLPACPVDCIELHPTG
>Mature_156_residues
MRNNAGIAGICCTEMAATSAPAPDLVERLDRLLPQTQCGQCGYDGCRPYAQAMADGQATIDHCPPGGDAGARALAQVLGV
PARPYDRSRGAHKLPQVAWIVEADCIGCTKCIQACPVDAIVGGAKHMHTVIAPLCTGCELCLPACPVDCIELHPTG

Specific function: Required for nitrogen fixation. May be part of a membrane complex functioning as an intermediate in the electron transport to nitrogenase [H]

COG id: COG2878

COG function: function code C; Predicted NADH:ubiquinone oxidoreductase, subunit RnfB

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1787915, Length=133, Percent_Identity=52.6315789473684, Blast_Score=140, Evalue=3e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR010207
- InterPro:   IPR016463
- InterPro:   IPR007202
- InterPro:   IPR012285 [H]

Pfam domain/function: PF00037 Fer4; PF04060 FeS [H]

EC number: NA

Molecular weight: Translated: 16181; Mature: 16181

Theoretical pI: Translated: 5.96; Mature: 5.96

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

9.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
12.2 %Cys+Met (Translated Protein)
9.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
12.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRNNAGIAGICCTEMAATSAPAPDLVERLDRLLPQTQCGQCGYDGCRPYAQAMADGQATI
CCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCEE
DHCPPGGDAGARALAQVLGVPARPYDRSRGAHKLPQVAWIVEADCIGCTKCIQACPVDAI
CCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHEECCCCHHHHHHHHHHCCHHHH
VGGAKHMHTVIAPLCTGCELCLPACPVDCIELHPTG
HCCHHHHHHHHHHHHCCHHHHHCCCCCCEEECCCCC
>Mature Secondary Structure
MRNNAGIAGICCTEMAATSAPAPDLVERLDRLLPQTQCGQCGYDGCRPYAQAMADGQATI
CCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCEE
DHCPPGGDAGARALAQVLGVPARPYDRSRGAHKLPQVAWIVEADCIGCTKCIQACPVDAI
CCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHEECCCCHHHHHHHHHHCCHHHH
VGGAKHMHTVIAPLCTGCELCLPACPVDCIELHPTG
HCCHHHHHHHHHHHHCCHHHHHCCCCCCEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA