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The map label for this gene is eptA [H]

Identifier: 78046806

GI number: 78046806

Start: 1409189

End: 1410856

Strand: Reverse

Name: eptA [H]

Synonym: XCV1250

Alternate gene names: 78046806

Gene position: 1410856-1409189 (Counterclockwise)

Preceding gene: 78046807

Following gene: 78046805

Centisome position: 27.24

GC content: 62.89

Gene sequence:

>1668_bases
ATGAGTACCTGGTTGCCGGAACCGCGCAGGCGTGGTGGCCTGCGCGCGCTGACGTGGAGTACGCGGCCGACGGTGTCGAC
CGAAGCGTTGGTACTGCTGTCGAGCCTGTTTTTTGCGTTGGTGTGCAATCAGTTGTTCTGGCGCACGGCCATGGCCACGC
ATCCGGGCAGCATCGGGTTCGCGATCTCGTTGATGGCGCTGTTGGTGGCCGTGCATGCGCTGCTCCTGGGCATCCTGGTT
TGGCGCTGGAATGCAAAGCCGCTGCTGACGCTCTTGCTGTTCACCACCGCGCTGGCCGCGCATTACATGGACAGCTACAA
CGTCTATCTGGATGCGGACATGCTGCGCAACGTGCTGCATACCGATCACAAGGAATCGCGCGAGTTGCTCACGCCGGGCC
TGATCCTTCCGCTGGTGTGCTACTTCCTGATTCCGACTGCATTGCTGTGGCGCACACGTTTGCGCGCACGCAGTGCGGGC
AAGGCGGTGCTGGTGCGATCGGGCTTCCTGCTGGTGGTGGCGGTGGTGGGCGCTGCTGGCGCCATGCTCTCGTCGCAGGA
TATTTCCGCACTGATGCGCAATCACCGCGAAGCGCGTTATTTGGCCACGCCGATCAATTACATCGTGGCATTGCGGCAAA
ACCTCAAATCCGAATCGCCGATCAAGCGTGCTCCCAAACAGCCGATCGAACACGACGCCATGGCCACGCCGCGCGCGCCG
GGCAGCCGCCCGCGCTTGTTGCTGGTGGTGCTGGGGGAAACCGCACGCGCGCAGAACTGGGGACTCAACGGCTATGCCAG
ACAGACCACGCCGGAGCTGGCACAGGCAGGGGTGATCAACTTCCCCGACATGCATTCGTGCGGGACCAGCACCGAAGTCT
CGGTGCCCTGCATGTTCTCGCCCTACGGGCGTCGCGACTACAACGAAGACATGATTCGTGGCCATCAGTCGTTCCTGCAT
GTGCTGGAACATGCCGGCATTTCGACGCTGTGGCGCGACAATCAGTCCGGCTGCAAGGGCGTGTGCGACGGGTTGGAGAT
CCAGAAGCTGGACGATGCCACCACACCGGGCCTGTGCGCCGATGGCCGCTGCATGGACGAGATCCTGTTGAGCGACCTGG
CTGCGCAGGTGCGCGCAAAGCCTGGCGACCGCGTGGTGGTGCTGCACCAACTGGGCAGCCATGGCCCGAGCTACTTCGAA
CGGTACCCGGCGCAGTTCGAACGCTTCAAGCCGGTGTGCAAGACCGCGGATCTGGGCAGCTGCGGCCGCCAGGACATCGT
CAACGCCTACGATAATTCGATCCTGTACACCGACCATTTCCTCAATCAGGCCATCCACGCGCTGCGTGGCCTGCCGGACT
ACGACACGGCGATGATCTATCTCTCCGATCACGGCGAATCGCTTGGCGAAAAAGGCCTGTACCTGCATGGCGTTCCGTAT
GCCATCGCGCCCAAGGAGCAGACCCATGTGCCGATGGTGATGTGGTTCTCGCCTGAGTTCGCGCGCGATCGCGGCCTGGA
TGAAGCCTGCCTGCGCCACCGTGCAGGGCAATATACCGATCAGGATGCGCTGTTCCCGTCCGTGCTTGGGTTGATGCAGG
TCAAGACCAGCGTCTATGCGCCCGAGCGCGACCTGTTCGCCAAGTGCACCGGCACCACGCTGCGCTGA

Upstream 100 bases:

>100_bases
CGCTGGCGGTGTGCTGGGTGAGCGCACTGGCGCTGTACTGCGTGATGCTGCAACGGCCGCGTCGCGCGCTTGGATTGATG
TTGCTCTCGGGGGATGCAGC

Downstream 100 bases:

>100_bases
TCGCCAACGCCTGCGTCTGCCCGCGCCGCACATCGCATGCGTGGCGTGCGTTGCCCCTTGCCCTGAACCCCGGCGCGCTC
TAGCCTTCGCCGGACTTCAC

Product: putative membrane-associated hydrolase

Products: NA

Alternate protein names: Polymyxin resistance protein pmrC [H]

Number of amino acids: Translated: 555; Mature: 554

Protein sequence:

>555_residues
MSTWLPEPRRRGGLRALTWSTRPTVSTEALVLLSSLFFALVCNQLFWRTAMATHPGSIGFAISLMALLVAVHALLLGILV
WRWNAKPLLTLLLFTTALAAHYMDSYNVYLDADMLRNVLHTDHKESRELLTPGLILPLVCYFLIPTALLWRTRLRARSAG
KAVLVRSGFLLVVAVVGAAGAMLSSQDISALMRNHREARYLATPINYIVALRQNLKSESPIKRAPKQPIEHDAMATPRAP
GSRPRLLLVVLGETARAQNWGLNGYARQTTPELAQAGVINFPDMHSCGTSTEVSVPCMFSPYGRRDYNEDMIRGHQSFLH
VLEHAGISTLWRDNQSGCKGVCDGLEIQKLDDATTPGLCADGRCMDEILLSDLAAQVRAKPGDRVVVLHQLGSHGPSYFE
RYPAQFERFKPVCKTADLGSCGRQDIVNAYDNSILYTDHFLNQAIHALRGLPDYDTAMIYLSDHGESLGEKGLYLHGVPY
AIAPKEQTHVPMVMWFSPEFARDRGLDEACLRHRAGQYTDQDALFPSVLGLMQVKTSVYAPERDLFAKCTGTTLR

Sequences:

>Translated_555_residues
MSTWLPEPRRRGGLRALTWSTRPTVSTEALVLLSSLFFALVCNQLFWRTAMATHPGSIGFAISLMALLVAVHALLLGILV
WRWNAKPLLTLLLFTTALAAHYMDSYNVYLDADMLRNVLHTDHKESRELLTPGLILPLVCYFLIPTALLWRTRLRARSAG
KAVLVRSGFLLVVAVVGAAGAMLSSQDISALMRNHREARYLATPINYIVALRQNLKSESPIKRAPKQPIEHDAMATPRAP
GSRPRLLLVVLGETARAQNWGLNGYARQTTPELAQAGVINFPDMHSCGTSTEVSVPCMFSPYGRRDYNEDMIRGHQSFLH
VLEHAGISTLWRDNQSGCKGVCDGLEIQKLDDATTPGLCADGRCMDEILLSDLAAQVRAKPGDRVVVLHQLGSHGPSYFE
RYPAQFERFKPVCKTADLGSCGRQDIVNAYDNSILYTDHFLNQAIHALRGLPDYDTAMIYLSDHGESLGEKGLYLHGVPY
AIAPKEQTHVPMVMWFSPEFARDRGLDEACLRHRAGQYTDQDALFPSVLGLMQVKTSVYAPERDLFAKCTGTTLR
>Mature_554_residues
STWLPEPRRRGGLRALTWSTRPTVSTEALVLLSSLFFALVCNQLFWRTAMATHPGSIGFAISLMALLVAVHALLLGILVW
RWNAKPLLTLLLFTTALAAHYMDSYNVYLDADMLRNVLHTDHKESRELLTPGLILPLVCYFLIPTALLWRTRLRARSAGK
AVLVRSGFLLVVAVVGAAGAMLSSQDISALMRNHREARYLATPINYIVALRQNLKSESPIKRAPKQPIEHDAMATPRAPG
SRPRLLLVVLGETARAQNWGLNGYARQTTPELAQAGVINFPDMHSCGTSTEVSVPCMFSPYGRRDYNEDMIRGHQSFLHV
LEHAGISTLWRDNQSGCKGVCDGLEIQKLDDATTPGLCADGRCMDEILLSDLAAQVRAKPGDRVVVLHQLGSHGPSYFER
YPAQFERFKPVCKTADLGSCGRQDIVNAYDNSILYTDHFLNQAIHALRGLPDYDTAMIYLSDHGESLGEKGLYLHGVPYA
IAPKEQTHVPMVMWFSPEFARDRGLDEACLRHRAGQYTDQDALFPSVLGLMQVKTSVYAPERDLFAKCTGTTLR

Specific function: Catalyzes the addition of a phosphoethanolamine moiety to the lipid A. The phosphoethanolamine modification is required for resistance to polymyxin [H]

COG id: COG2194

COG function: function code R; Predicted membrane-associated, metal-dependent hydrolase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoethanolamine transferase family. EptA subfamily [H]

Homologues:

Organism=Escherichia coli, GI87082372, Length=537, Percent_Identity=36.3128491620112, Blast_Score=360, Evalue=1e-100,
Organism=Escherichia coli, GI87082286, Length=450, Percent_Identity=30, Blast_Score=162, Evalue=4e-41,
Organism=Escherichia coli, GI87082223, Length=399, Percent_Identity=26.8170426065163, Blast_Score=89, Evalue=5e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017849
- InterPro:   IPR017850
- InterPro:   IPR012549
- InterPro:   IPR000917 [H]

Pfam domain/function: PF08019 DUF1705; PF00884 Sulfatase [H]

EC number: NA

Molecular weight: Translated: 61642; Mature: 61511

Theoretical pI: Translated: 8.30; Mature: 8.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTWLPEPRRRGGLRALTWSTRPTVSTEALVLLSSLFFALVCNQLFWRTAMATHPGSIGF
CCCCCCCHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
AISLMALLVAVHALLLGILVWRWNAKPLLTLLLFTTALAAHYMDSYNVYLDADMLRNVLH
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCEEECHHHHHHHHH
TDHKESRELLTPGLILPLVCYFLIPTALLWRTRLRARSAGKAVLVRSGFLLVVAVVGAAG
CCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHH
AMLSSQDISALMRNHREARYLATPINYIVALRQNLKSESPIKRAPKQPIEHDAMATPRAP
HHHCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCCCCCCCCCCCC
GSRPRLLLVVLGETARAQNWGLNGYARQTTPELAQAGVINFPDMHSCGTSTEVSVPCMFS
CCCCCEEEEEECCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCEECCEEEC
PYGRRDYNEDMIRGHQSFLHVLEHAGISTLWRDNQSGCKGVCDGLEIQKLDDATTPGLCA
CCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHCCCCCCHHHHHCCCCEEECCCCCCCCCCC
DGRCMDEILLSDLAAQVRAKPGDRVVVLHQLGSHGPSYFERYPAQFERFKPVCKTADLGS
CCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHCHHHHHHHHHHHCCCCCCC
CGRQDIVNAYDNSILYTDHFLNQAIHALRGLPDYDTAMIYLSDHGESLGEKGLYLHGVPY
CCHHHHHHHHCCCEEEHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCEEEECCCE
AIAPKEQTHVPMVMWFSPEFARDRGLDEACLRHRAGQYTDQDALFPSVLGLMQVKTSVYA
EECCCCCCCCCEEEEECCHHHHHCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCC
PERDLFAKCTGTTLR
CCHHHHHHCCCCCCC
>Mature Secondary Structure 
STWLPEPRRRGGLRALTWSTRPTVSTEALVLLSSLFFALVCNQLFWRTAMATHPGSIGF
CCCCCCHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
AISLMALLVAVHALLLGILVWRWNAKPLLTLLLFTTALAAHYMDSYNVYLDADMLRNVLH
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCEEECHHHHHHHHH
TDHKESRELLTPGLILPLVCYFLIPTALLWRTRLRARSAGKAVLVRSGFLLVVAVVGAAG
CCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHH
AMLSSQDISALMRNHREARYLATPINYIVALRQNLKSESPIKRAPKQPIEHDAMATPRAP
HHHCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCCCCCCCCCCCC
GSRPRLLLVVLGETARAQNWGLNGYARQTTPELAQAGVINFPDMHSCGTSTEVSVPCMFS
CCCCCEEEEEECCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCEECCEEEC
PYGRRDYNEDMIRGHQSFLHVLEHAGISTLWRDNQSGCKGVCDGLEIQKLDDATTPGLCA
CCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHCCCCCCHHHHHCCCCEEECCCCCCCCCCC
DGRCMDEILLSDLAAQVRAKPGDRVVVLHQLGSHGPSYFERYPAQFERFKPVCKTADLGS
CCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHCHHHHHHHHHHHCCCCCCC
CGRQDIVNAYDNSILYTDHFLNQAIHALRGLPDYDTAMIYLSDHGESLGEKGLYLHGVPY
CCHHHHHHHHCCCEEEHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCEEEECCCE
AIAPKEQTHVPMVMWFSPEFARDRGLDEACLRHRAGQYTDQDALFPSVLGLMQVKTSVYA
EECCCCCCCCCEEEEECCHHHHHCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCC
PERDLFAKCTGTTLR
CCHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8391535; 11677609 [H]