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The map label for this gene is prfA [H]

Identifier: 78046531

GI number: 78046531

Start: 1105013

End: 1106098

Strand: Reverse

Name: prfA [H]

Synonym: XCV0975

Alternate gene names: 78046531

Gene position: 1106098-1105013 (Counterclockwise)

Preceding gene: 78046532

Following gene: 78046530

Centisome position: 21.36

GC content: 65.19

Gene sequence:

>1086_bases
ATGACGCCGACCCTGCGCCGTAAGCTCGAAGCGCTGGCCGAGCGCCGCGAAGAACTGCAGCACCTGCTCTCCGACCCCGA
TGTGGTGGGCAACAACGACACGTTCCGTACGCTCTCGCGCGAACTGTCGCAACTGGAGCCGGTGGCGATGGCGCTGGAAG
AGGAGGCGCGTGCCAAGGCCGACCTGGCCACGGCCGAAGCGCTGCGCAACGACCCGGAGATGCGCGAACTGGCCGAAGAG
GAAATCGCCGCTGCACAGGCGCGCCTGGAACAACTCGATGCACAACTTGCATCGCTGCTGGTACCGCGTGATCCGCGCGA
CGACGGCAACCTGTTTCTGGAAGTGCGCGCCGGCACTGGCGGTGACGAAGCGGCGATCTTTGCCGGCGACCTGTTCCGCA
TGTACGCACGCTACGCCGAGCGCCAGGGCTGGAAGGTGGAAATCGAATCCGACAGCCCCGGCGAGCATGGCGGCTACAAG
GAAGTGGTGGCGCGGGTGGTCGGGCGCGGTGCGTATTCGCGCCTGAAATTCGAATCCGGCACCCATCGCGTGCAACGCGT
GCCGGCAACCGAATCGCAGGGCCGTATCCACACCTCGGCTGCCACGGTGGCGATCATTCCCGAAGCCGACGACGTGGAAG
AGATCACGATCAATCCGGCCGATCTGAAGGTGGACACGTTCCGCTCGTCCGGTGCGGGCGGCCAGCACGTCAACAAGACC
GAATCGGCAATCCGCATCACCCACGTGCCCAGCGGGGTGGTGGTGGAATGCCAGACCGAGCGCAGCCAGCATGCCAATCG
CGACAAGGCGATGAAGCGGTTGAAGGCGCAGCTGCTGGACGCCGAACGCAGCAAGGCGGCAGCGGCCGAAGCGCAGACGC
GCAAGTTGCAGGTGGGCAGCGGCGACCGTAGCCAGCGCATCCGCACCTACAGTTTTCCGCAGGGTCGCATCACCGACCAT
CGCGTCGAAGGGCTGACCTTGTACGACCTGCCCAACATCATCGAAGGCGATCTGGATGCGTTGATCGGGCGGCTGCTGCA
CGAACATCAAGCCGACGAACTGGCACGGTTGAGCGATAGCCCGTGA

Upstream 100 bases:

>100_bases
CTCAACAACGACCTGGAATTGACCACTGCGGCCGACCGGCTGTTTCCGGAAAAGCCGGGTTACCAACACCCCCCCATAGC
TACCCCGATCGTGAGGACCG

Downstream 100 bases:

>100_bases
GTGCGCAAGTCCCGCGCGAGCTGCTGCAGCTGCGCGAGCAAGTGCGACGCCAGCCCGGCGATTTCGTCGCCTGGTTGATG
CTGGCCGATGCCGAGCTGGG

Product: peptide chain release factor 1

Products: NA

Alternate protein names: RF-1 [H]

Number of amino acids: Translated: 361; Mature: 360

Protein sequence:

>361_residues
MTPTLRRKLEALAERREELQHLLSDPDVVGNNDTFRTLSRELSQLEPVAMALEEEARAKADLATAEALRNDPEMRELAEE
EIAAAQARLEQLDAQLASLLVPRDPRDDGNLFLEVRAGTGGDEAAIFAGDLFRMYARYAERQGWKVEIESDSPGEHGGYK
EVVARVVGRGAYSRLKFESGTHRVQRVPATESQGRIHTSAATVAIIPEADDVEEITINPADLKVDTFRSSGAGGQHVNKT
ESAIRITHVPSGVVVECQTERSQHANRDKAMKRLKAQLLDAERSKAAAAEAQTRKLQVGSGDRSQRIRTYSFPQGRITDH
RVEGLTLYDLPNIIEGDLDALIGRLLHEHQADELARLSDSP

Sequences:

>Translated_361_residues
MTPTLRRKLEALAERREELQHLLSDPDVVGNNDTFRTLSRELSQLEPVAMALEEEARAKADLATAEALRNDPEMRELAEE
EIAAAQARLEQLDAQLASLLVPRDPRDDGNLFLEVRAGTGGDEAAIFAGDLFRMYARYAERQGWKVEIESDSPGEHGGYK
EVVARVVGRGAYSRLKFESGTHRVQRVPATESQGRIHTSAATVAIIPEADDVEEITINPADLKVDTFRSSGAGGQHVNKT
ESAIRITHVPSGVVVECQTERSQHANRDKAMKRLKAQLLDAERSKAAAAEAQTRKLQVGSGDRSQRIRTYSFPQGRITDH
RVEGLTLYDLPNIIEGDLDALIGRLLHEHQADELARLSDSP
>Mature_360_residues
TPTLRRKLEALAERREELQHLLSDPDVVGNNDTFRTLSRELSQLEPVAMALEEEARAKADLATAEALRNDPEMRELAEEE
IAAAQARLEQLDAQLASLLVPRDPRDDGNLFLEVRAGTGGDEAAIFAGDLFRMYARYAERQGWKVEIESDSPGEHGGYKE
VVARVVGRGAYSRLKFESGTHRVQRVPATESQGRIHTSAATVAIIPEADDVEEITINPADLKVDTFRSSGAGGQHVNKTE
SAIRITHVPSGVVVECQTERSQHANRDKAMKRLKAQLLDAERSKAAAAEAQTRKLQVGSGDRSQRIRTYSFPQGRITDHR
VEGLTLYDLPNIIEGDLDALIGRLLHEHQADELARLSDSP

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA [H]

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=277, Percent_Identity=48.3754512635379, Blast_Score=273, Evalue=2e-73,
Organism=Homo sapiens, GI34577120, Length=368, Percent_Identity=36.9565217391304, Blast_Score=244, Evalue=9e-65,
Organism=Homo sapiens, GI166795305, Length=179, Percent_Identity=49.7206703910614, Blast_Score=186, Evalue=3e-47,
Organism=Escherichia coli, GI1787462, Length=359, Percent_Identity=60.7242339832869, Blast_Score=426, Evalue=1e-120,
Organism=Escherichia coli, GI2367172, Length=335, Percent_Identity=39.7014925373134, Blast_Score=226, Evalue=2e-60,
Organism=Caenorhabditis elegans, GI17542784, Length=321, Percent_Identity=34.2679127725857, Blast_Score=186, Evalue=1e-47,
Organism=Saccharomyces cerevisiae, GI6321295, Length=286, Percent_Identity=45.4545454545455, Blast_Score=238, Evalue=1e-63,
Organism=Drosophila melanogaster, GI19921226, Length=338, Percent_Identity=41.4201183431953, Blast_Score=243, Evalue=1e-64,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 39880; Mature: 39749

Theoretical pI: Translated: 5.15; Mature: 5.15

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPTLRRKLEALAERREELQHLLSDPDVVGNNDTFRTLSRELSQLEPVAMALEEEARAKA
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DLATAEALRNDPEMRELAEEEIAAAQARLEQLDAQLASLLVPRDPRDDGNLFLEVRAGTG
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCC
GDEAAIFAGDLFRMYARYAERQGWKVEIESDSPGEHGGYKEVVARVVGRGAYSRLKFESG
CCCCEEHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCHHHHHHHHHCCCHHHHEEECCC
THRVQRVPATESQGRIHTSAATVAIIPEADDVEEITINPADLKVDTFRSSGAGGQHVNKT
CHHHHCCCCCCCCCEEEECCEEEEEECCCCCCCEEEECCCCEEEECCCCCCCCCCCCCCC
ESAIRITHVPSGVVVECQTERSQHANRDKAMKRLKAQLLDAERSKAAAAEAQTRKLQVGS
CCEEEEEECCCCEEEEEECHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCC
GDRSQRIRTYSFPQGRITDHRVEGLTLYDLPNIIEGDLDALIGRLLHEHQADELARLSDS
CCCCCCEEEECCCCCCCCCCCCCCEEEECCCCHHHCCHHHHHHHHHHHHHHHHHHHCCCC
P
C
>Mature Secondary Structure 
TPTLRRKLEALAERREELQHLLSDPDVVGNNDTFRTLSRELSQLEPVAMALEEEARAKA
CHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DLATAEALRNDPEMRELAEEEIAAAQARLEQLDAQLASLLVPRDPRDDGNLFLEVRAGTG
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCC
GDEAAIFAGDLFRMYARYAERQGWKVEIESDSPGEHGGYKEVVARVVGRGAYSRLKFESG
CCCCEEHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCHHHHHHHHHCCCHHHHEEECCC
THRVQRVPATESQGRIHTSAATVAIIPEADDVEEITINPADLKVDTFRSSGAGGQHVNKT
CHHHHCCCCCCCCCEEEECCEEEEEECCCCCCCEEEECCCCEEEECCCCCCCCCCCCCCC
ESAIRITHVPSGVVVECQTERSQHANRDKAMKRLKAQLLDAERSKAAAAEAQTRKLQVGS
CCEEEEEECCCCEEEEEECHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCC
GDRSQRIRTYSFPQGRITDHRVEGLTLYDLPNIIEGDLDALIGRLLHEHQADELARLSDS
CCCCCCEEEECCCCCCCCCCCCCCEEEECCCCHHHCCHHHHHHHHHHHHHHHHHHHCCCC
P
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12024217 [H]