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The map label for this gene is ahpF [H]

Identifier: 78046498

GI number: 78046498

Start: 1069789

End: 1071381

Strand: Reverse

Name: ahpF [H]

Synonym: XCV0942

Alternate gene names: 78046498

Gene position: 1071381-1069789 (Counterclockwise)

Preceding gene: 78046499

Following gene: 78046497

Centisome position: 20.69

GC content: 64.91

Gene sequence:

>1593_bases
ATGTTGGATGCCAATCTCAAGACCCAGCTGACCGCCTACCTGGAACGGGTGACGCGGCCGATTCAAATCAACGCCTCGAT
CGACGACAGTGCCGGCTCGCGCGAAATGCTCGACCTGCTGGAAGAGTTGGTCCTGCTCTCGGACAAGATCAGCCTGGACA
TCCACCGCGACGACAACCAGCGCAAGCCGTCGTTCGCACTGACCACGCCGGGCCAGGACATTTCGCTGCGCTTTGCCGGG
CTGCCGATGGGCCACGAGTTCACCTCGCTGGTGCTGGCGCTGCTGCAGGTGGGCGGCCACCCGTCCAAGGCGGCAGCCGA
ACTGATCGAGCAGGTGCAGCACCTGGAGGGCGACTACCAGTTCGAAACCTATTTCTCGCTGTCGTGCCAGAACTGCCCGG
ACGTGGTGCAGGCGCTGAACCTGGCGGCCGTGCTCAACCCGCGCATCAAGCACGTGGCCATCGACGGTGCCTGGTTCCAG
GACGAAGTGGAGGCCCGCCAGATCATGTCGGTGCCCACCGTGTACTTGAACGGCGAGCTGTTCGACCAGGGCCGCATGAC
GCTGGAGCAGATTGTCGCCAAGCTCGACACCAATGCCGCCAAGCGCGACGCTGCGAAAATCGCCGCGAAAGAAGCGTTCG
ACGTTTTGGTGGTGGGCGGTGGCCCGGCCGGTTCGGCAGCGGCGGTGTATGCCGCACGCAAGGGCATCCGCACCGGCGTG
GCGGCCGAGCGTTTCGGTGGCCAGGTGCTGGACACCATGTCGATCGAAAACTTCATCTCCGTGCCGGAAACCGAAGGCCC
GAAGATGGCCGCCGCGCTCGAGCAGCACGTGCGCCAATACGACGTGGACATCATGAACCTGCAGCGTGCCGAGCAGCTGA
TTCCGGCCGGTGCCGACGGCTTGATCGAAATCAAGCTGGCCAATGGCGCCTCGCTCAAGAGCAAGACCGTGATCCTGTCC
ACCGGCGCGCGCTGGAGGCAGATGAACGTGCCCGGCGAAGACCAGTACAAGAACAAGGGCGTGGCGTACTGCCCGCACTG
CGACGGCCCGCTGTTCAAGGGCAAGCGCGTGGCGGTGATCGGCGGAGGCAACTCCGGCGTGGAAGCGGCGATCGATCTGG
CCGGCATCGTGGCGCATGTCACGCTGGTGGAATTCGACGACAAGCTGCGCGCCGACGAAGTGTTGCAACGCAAGCTGCGC
AGCCTGCACAACGTGCGCATCATCACCAGCGCGCAGACCACTGAGGTGCTGGGCGACGGGCAGAAGGTGACCGGTCTGGT
CTACAAGGACCGCACCGGCGGCGATATCCAGCACATCGAGCTGGAAGGTGTGTTCGTGCAGATCGGCCTGCTGCCCAACA
CCGAGTTCCTGCGCGGCACGGTGGCGCTGTCGCCGCGCGGCGAAATCATCGTCGACGACCGTGGCCAGACCGATGTGCCC
GGCGTGTTCGCCGCGGGCGACGCCACCACGGTGCCGTACAAGCAGATCGTGATCGCGATGGGCGAAGGTTCCAAGGCCGC
GTTAAGCGCATTCGACCATCTGATCCGCACCAGCGCACCGGCAACTGCCGACAGCGTGGCCCAGGCGGCATAA

Upstream 100 bases:

>100_bases
CCGACCTCGCGCCGGCTGCGGTTCACTCCCTTCCTTCCCGGCGACCTGCCTCAGCGCAGCGACGCCACGCCCTCCCCTTG
CCCATTCCCAGGAGTTCTCC

Downstream 100 bases:

>100_bases
GCGAGCGCCTGCTTAGCGCCCGGCCGACCCGGGCGCTAAGCTTGCAACACGCGCCAGTCGTTCCCCGTCACCGCAATGAA
GGCTGCTGCACATGAATCTG

Product: Alkyl hydroperoxide reductase subunit F

Products: an alcohol; H2O; NAD [C]

Alternate protein names: NA

Number of amino acids: Translated: 530; Mature: 530

Protein sequence:

>530_residues
MLDANLKTQLTAYLERVTRPIQINASIDDSAGSREMLDLLEELVLLSDKISLDIHRDDNQRKPSFALTTPGQDISLRFAG
LPMGHEFTSLVLALLQVGGHPSKAAAELIEQVQHLEGDYQFETYFSLSCQNCPDVVQALNLAAVLNPRIKHVAIDGAWFQ
DEVEARQIMSVPTVYLNGELFDQGRMTLEQIVAKLDTNAAKRDAAKIAAKEAFDVLVVGGGPAGSAAAVYAARKGIRTGV
AAERFGGQVLDTMSIENFISVPETEGPKMAAALEQHVRQYDVDIMNLQRAEQLIPAGADGLIEIKLANGASLKSKTVILS
TGARWRQMNVPGEDQYKNKGVAYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVAHVTLVEFDDKLRADEVLQRKLR
SLHNVRIITSAQTTEVLGDGQKVTGLVYKDRTGGDIQHIELEGVFVQIGLLPNTEFLRGTVALSPRGEIIVDDRGQTDVP
GVFAAGDATTVPYKQIVIAMGEGSKAALSAFDHLIRTSAPATADSVAQAA

Sequences:

>Translated_530_residues
MLDANLKTQLTAYLERVTRPIQINASIDDSAGSREMLDLLEELVLLSDKISLDIHRDDNQRKPSFALTTPGQDISLRFAG
LPMGHEFTSLVLALLQVGGHPSKAAAELIEQVQHLEGDYQFETYFSLSCQNCPDVVQALNLAAVLNPRIKHVAIDGAWFQ
DEVEARQIMSVPTVYLNGELFDQGRMTLEQIVAKLDTNAAKRDAAKIAAKEAFDVLVVGGGPAGSAAAVYAARKGIRTGV
AAERFGGQVLDTMSIENFISVPETEGPKMAAALEQHVRQYDVDIMNLQRAEQLIPAGADGLIEIKLANGASLKSKTVILS
TGARWRQMNVPGEDQYKNKGVAYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVAHVTLVEFDDKLRADEVLQRKLR
SLHNVRIITSAQTTEVLGDGQKVTGLVYKDRTGGDIQHIELEGVFVQIGLLPNTEFLRGTVALSPRGEIIVDDRGQTDVP
GVFAAGDATTVPYKQIVIAMGEGSKAALSAFDHLIRTSAPATADSVAQAA
>Mature_530_residues
MLDANLKTQLTAYLERVTRPIQINASIDDSAGSREMLDLLEELVLLSDKISLDIHRDDNQRKPSFALTTPGQDISLRFAG
LPMGHEFTSLVLALLQVGGHPSKAAAELIEQVQHLEGDYQFETYFSLSCQNCPDVVQALNLAAVLNPRIKHVAIDGAWFQ
DEVEARQIMSVPTVYLNGELFDQGRMTLEQIVAKLDTNAAKRDAAKIAAKEAFDVLVVGGGPAGSAAAVYAARKGIRTGV
AAERFGGQVLDTMSIENFISVPETEGPKMAAALEQHVRQYDVDIMNLQRAEQLIPAGADGLIEIKLANGASLKSKTVILS
TGARWRQMNVPGEDQYKNKGVAYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVAHVTLVEFDDKLRADEVLQRKLR
SLHNVRIITSAQTTEVLGDGQKVTGLVYKDRTGGDIQHIELEGVFVQIGLLPNTEFLRGTVALSPRGEIIVDDRGQTDVP
GVFAAGDATTVPYKQIVIAMGEGSKAALSAFDHLIRTSAPATADSVAQAA

Specific function: Serves to protect the cell against DNA damage by alkyl hydroperoxides. It can use either NADH or NADPH as electron donor for direct reduction of redox dyes or of alkyl hydroperoxides when combined with the AhpC protein [H]

COG id: COG3634

COG function: function code O; Alkyl hydroperoxide reductase, large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Escherichia coli, GI87081763, Length=521, Percent_Identity=64.8752399232246, Blast_Score=692, Evalue=0.0,
Organism=Escherichia coli, GI1787114, Length=314, Percent_Identity=34.0764331210191, Blast_Score=159, Evalue=4e-40,
Organism=Saccharomyces cerevisiae, GI6321898, Length=340, Percent_Identity=30, Blast_Score=148, Evalue=3e-36,
Organism=Saccharomyces cerevisiae, GI6320560, Length=313, Percent_Identity=30.9904153354633, Blast_Score=144, Evalue=4e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012081
- InterPro:   IPR013027
- InterPro:   IPR002109
- InterPro:   IPR008255
- InterPro:   IPR001327
- InterPro:   IPR000103
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.6.4.- [C]

Molecular weight: Translated: 56924; Mature: 56924

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: PS00573 PYRIDINE_REDOX_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDANLKTQLTAYLERVTRPIQINASIDDSAGSREMLDLLEELVLLSDKISLDIHRDDNQ
CCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCC
RKPSFALTTPGQDISLRFAGLPMGHEFTSLVLALLQVGGHPSKAAAELIEQVQHLEGDYQ
CCCCEEEECCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCEE
FETYFSLSCQNCPDVVQALNLAAVLNPRIKHVAIDGAWFQDEVEARQIMSVPTVYLNGEL
EEEEEEECCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHCCCEEEECCEE
FDQGRMTLEQIVAKLDTNAAKRDAAKIAAKEAFDVLVVGGGPAGSAAAVYAARKGIRTGV
ECCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCEEEEEECCCCCCCHHHHHHHHHCHHCCC
AAERFGGQVLDTMSIENFISVPETEGPKMAAALEQHVRQYDVDIMNLQRAEQLIPAGADG
HHHHHCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCC
LIEIKLANGASLKSKTVILSTGARWRQMNVPGEDQYKNKGVAYCPHCDGPLFKGKRVAVI
EEEEEECCCCCCCCCEEEEECCCCEEECCCCCCHHHCCCCEEECCCCCCCCCCCCEEEEE
GGGNSGVEAAIDLAGIVAHVTLVEFDDKLRADEVLQRKLRSLHNVRIITSAQTTEVLGDG
ECCCCCHHHHHHHHHHHHHEEEEECCCCCCHHHHHHHHHHHHCCEEEEECCCHHHHHCCC
QKVTGLVYKDRTGGDIQHIELEGVFVQIGLLPNTEFLRGTVALSPRGEIIVDDRGQTDVP
CEEEEEEEECCCCCCEEEEEEEEEEEEEEECCCCHHHEEEEEECCCCCEEECCCCCCCCC
GVFAAGDATTVPYKQIVIAMGEGSKAALSAFDHLIRTSAPATADSVAQAA
CEEECCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHCCCCCCHHHHHHCC
>Mature Secondary Structure
MLDANLKTQLTAYLERVTRPIQINASIDDSAGSREMLDLLEELVLLSDKISLDIHRDDNQ
CCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCC
RKPSFALTTPGQDISLRFAGLPMGHEFTSLVLALLQVGGHPSKAAAELIEQVQHLEGDYQ
CCCCEEEECCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCEE
FETYFSLSCQNCPDVVQALNLAAVLNPRIKHVAIDGAWFQDEVEARQIMSVPTVYLNGEL
EEEEEEECCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHCCCEEEECCEE
FDQGRMTLEQIVAKLDTNAAKRDAAKIAAKEAFDVLVVGGGPAGSAAAVYAARKGIRTGV
ECCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCEEEEEECCCCCCCHHHHHHHHHCHHCCC
AAERFGGQVLDTMSIENFISVPETEGPKMAAALEQHVRQYDVDIMNLQRAEQLIPAGADG
HHHHHCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCC
LIEIKLANGASLKSKTVILSTGARWRQMNVPGEDQYKNKGVAYCPHCDGPLFKGKRVAVI
EEEEEECCCCCCCCCEEEEECCCCEEECCCCCCHHHCCCCEEECCCCCCCCCCCCEEEEE
GGGNSGVEAAIDLAGIVAHVTLVEFDDKLRADEVLQRKLRSLHNVRIITSAQTTEVLGDG
ECCCCCHHHHHHHHHHHHHEEEEECCCCCCHHHHHHHHHHHHCCEEEEECCCHHHHHCCC
QKVTGLVYKDRTGGDIQHIELEGVFVQIGLLPNTEFLRGTVALSPRGEIIVDDRGQTDVP
CEEEEEEEECCCCCCEEEEEEEEEEEEEEECCCCHHHEEEEEECCCCCEEECCCCCCCCC
GVFAAGDATTVPYKQIVIAMGEGSKAALSAFDHLIRTSAPATADSVAQAA
CEEECCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHCCCCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ROOH; Proton; NADH [C]

Specific reaction: ROOH + Proton + NADH = an alcohol + H2O + NAD [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9190810 [H]