Click here to switch to the map view.

The map label for this gene is metK [H]

Identifier: 78046421

GI number: 78046421

Start: 993440

End: 994651

Strand: Reverse

Name: metK [H]

Synonym: XCV0865

Alternate gene names: 78046421

Gene position: 994651-993440 (Counterclockwise)

Preceding gene: 78046423

Following gene: 78046412

Centisome position: 19.21

GC content: 64.19

Gene sequence:

>1212_bases
ATGTCCAGCTACCTGTTCACCTCCGAATCGGTCTCCGAAGGCCATCCGGACAAGATCGCCGACCAGATCTCCGACGCAGT
GCTGGACGCCATCCTGGCCCAGGACAAGCGCGCCCGCGTGGCCTGCGAGACGATGGTCAAGACCGGCGTTGCGATCGTGG
CCGGCGAAGTGACCACCAGTGCCTGGATCGATCTGGAAGCGCTGACCCGCAAGGTCATCCTGGACATCGGCTACAACAGC
TCCGACGTCGGCTTCGACGGCGAGACCTGTGGCGTGCTCAACCTGATCGGCAAGCAGTCGCCGGACATCAACCAGGGCGT
GGATCGCAAGAATCCCGAGCAGCAGGGTGCGGGCGACCAGGGCCTGATGTTCGGCTATGCCACCAACGAAACCGACAGCT
TCATGCCGGCGGCGATCCACCTCTCGCACCGCCTGGTCGAGCAGCAGGCCAAGATCCGCAAGAAGAAGAACTCGGCGCTG
TCGTGGCTGCGTCCAGACGCCAAGTCGCAGGTCACCCTGCGCTATGAAGATGGCGTGGCGACGGCGATCGATGCAGTGGT
GCTGTCGACCCAGCACGACCCGGGCGTGAAGCAGAAGGACCTGATCGAAGCGGTGCGCGAGGAAATCCTCAAGCCGGTGC
TGCCGGCCAAGTGGCTGCACAAGGGCACCAAGTTCCACATCAACCCGACCGGCAAGTTCGTGATCGGCGGCCCGGTGGGC
GATTGCGGCCTGACCGGCCGCAAGATCATCGTCGACACCTACGGCGGCTGGGCGCGTCACGGTGGCGGTGCGTTCTCCGG
CAAGGATCCGTCCAAGGTGGACCGTTCGGCTGCCTATGCCGCGCGTTATGTTGCCAAGAACGTGGTGGCCGCCGGTCTGG
CCGACCGTTGCGAAGTGCAGGTCTCCTACGCCATCGGCGTGGCCGAGCCGACCTCGATTTCGGTCACCACCTTCGGCACC
GGCAAGATCGCCGACGAGCAGATCGAAAAGCTGATCCGCAAGCATTTCGACCTGCGTCCGTTCGGCATCATCCAGATGCT
CGACCTGATCCACCCGATGTATCAGCAGACCGCGTCTTACGGCCACTTCGGCCGCAAGCCGAAGGACTTCACCTACACCG
ACGGCACCGGCGCGCAGCACAGCGCGACCTCGTTCTCGTGGGAAAAGACCGACCGCGCCGACGCACTGCGTGCCGCCGCC
AAGCTGAAATAA

Upstream 100 bases:

>100_bases
TAACCCCAAACCGATCCATTCAGGCGCACAATGCAACCGACACACGGGGCGTGTATCATCCGCACCCATCTTTTCATCCG
AATCAAGGGATATAAGCCTG

Downstream 100 bases:

>100_bases
TCGCCCCGCTCCTGCGAAAGCTAGGTAGACGCATAACGACTGGGCCGCTGATGCGGCCCAGTTGCGGTTTTGGGGTCGTC
GGTGGGTTGCCCACGCCAGG

Product: S-adenosylmethionine synthetase

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase [H]

Number of amino acids: Translated: 403; Mature: 402

Protein sequence:

>403_residues
MSSYLFTSESVSEGHPDKIADQISDAVLDAILAQDKRARVACETMVKTGVAIVAGEVTTSAWIDLEALTRKVILDIGYNS
SDVGFDGETCGVLNLIGKQSPDINQGVDRKNPEQQGAGDQGLMFGYATNETDSFMPAAIHLSHRLVEQQAKIRKKKNSAL
SWLRPDAKSQVTLRYEDGVATAIDAVVLSTQHDPGVKQKDLIEAVREEILKPVLPAKWLHKGTKFHINPTGKFVIGGPVG
DCGLTGRKIIVDTYGGWARHGGGAFSGKDPSKVDRSAAYAARYVAKNVVAAGLADRCEVQVSYAIGVAEPTSISVTTFGT
GKIADEQIEKLIRKHFDLRPFGIIQMLDLIHPMYQQTASYGHFGRKPKDFTYTDGTGAQHSATSFSWEKTDRADALRAAA
KLK

Sequences:

>Translated_403_residues
MSSYLFTSESVSEGHPDKIADQISDAVLDAILAQDKRARVACETMVKTGVAIVAGEVTTSAWIDLEALTRKVILDIGYNS
SDVGFDGETCGVLNLIGKQSPDINQGVDRKNPEQQGAGDQGLMFGYATNETDSFMPAAIHLSHRLVEQQAKIRKKKNSAL
SWLRPDAKSQVTLRYEDGVATAIDAVVLSTQHDPGVKQKDLIEAVREEILKPVLPAKWLHKGTKFHINPTGKFVIGGPVG
DCGLTGRKIIVDTYGGWARHGGGAFSGKDPSKVDRSAAYAARYVAKNVVAAGLADRCEVQVSYAIGVAEPTSISVTTFGT
GKIADEQIEKLIRKHFDLRPFGIIQMLDLIHPMYQQTASYGHFGRKPKDFTYTDGTGAQHSATSFSWEKTDRADALRAAA
KLK
>Mature_402_residues
SSYLFTSESVSEGHPDKIADQISDAVLDAILAQDKRARVACETMVKTGVAIVAGEVTTSAWIDLEALTRKVILDIGYNSS
DVGFDGETCGVLNLIGKQSPDINQGVDRKNPEQQGAGDQGLMFGYATNETDSFMPAAIHLSHRLVEQQAKIRKKKNSALS
WLRPDAKSQVTLRYEDGVATAIDAVVLSTQHDPGVKQKDLIEAVREEILKPVLPAKWLHKGTKFHINPTGKFVIGGPVGD
CGLTGRKIIVDTYGGWARHGGGAFSGKDPSKVDRSAAYAARYVAKNVVAAGLADRCEVQVSYAIGVAEPTSISVTTFGTG
KIADEQIEKLIRKHFDLRPFGIIQMLDLIHPMYQQTASYGHFGRKPKDFTYTDGTGAQHSATSFSWEKTDRADALRAAAK
LK

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family [H]

Homologues:

Organism=Homo sapiens, GI5174529, Length=381, Percent_Identity=54.3307086614173, Blast_Score=390, Evalue=1e-108,
Organism=Homo sapiens, GI4557737, Length=367, Percent_Identity=55.3133514986376, Blast_Score=387, Evalue=1e-107,
Organism=Escherichia coli, GI1789311, Length=403, Percent_Identity=70.2233250620347, Blast_Score=588, Evalue=1e-169,
Organism=Caenorhabditis elegans, GI17551082, Length=369, Percent_Identity=55.0135501355014, Blast_Score=393, Evalue=1e-109,
Organism=Caenorhabditis elegans, GI17538498, Length=368, Percent_Identity=55.7065217391304, Blast_Score=388, Evalue=1e-108,
Organism=Caenorhabditis elegans, GI17538494, Length=368, Percent_Identity=55.4347826086957, Blast_Score=385, Evalue=1e-107,
Organism=Caenorhabditis elegans, GI25145633, Length=376, Percent_Identity=54.5212765957447, Blast_Score=382, Evalue=1e-106,
Organism=Caenorhabditis elegans, GI32565905, Length=368, Percent_Identity=50, Blast_Score=324, Evalue=4e-89,
Organism=Caenorhabditis elegans, GI32565911, Length=318, Percent_Identity=52.8301886792453, Blast_Score=313, Evalue=8e-86,
Organism=Caenorhabditis elegans, GI25145635, Length=326, Percent_Identity=51.5337423312883, Blast_Score=307, Evalue=7e-84,
Organism=Caenorhabditis elegans, GI32565903, Length=295, Percent_Identity=52.2033898305085, Blast_Score=281, Evalue=4e-76,
Organism=Caenorhabditis elegans, GI32565909, Length=82, Percent_Identity=62.1951219512195, Blast_Score=108, Evalue=7e-24,
Organism=Caenorhabditis elegans, GI32565907, Length=46, Percent_Identity=71.7391304347826, Blast_Score=70, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6323209, Length=381, Percent_Identity=54.5931758530184, Blast_Score=383, Evalue=1e-107,
Organism=Saccharomyces cerevisiae, GI6320710, Length=381, Percent_Identity=54.0682414698163, Blast_Score=380, Evalue=1e-106,
Organism=Drosophila melanogaster, GI45552159, Length=369, Percent_Identity=53.3875338753388, Blast_Score=367, Evalue=1e-101,
Organism=Drosophila melanogaster, GI24580529, Length=369, Percent_Identity=53.3875338753388, Blast_Score=367, Evalue=1e-101,
Organism=Drosophila melanogaster, GI24580537, Length=369, Percent_Identity=53.3875338753388, Blast_Score=367, Evalue=1e-101,
Organism=Drosophila melanogaster, GI24580533, Length=369, Percent_Identity=53.3875338753388, Blast_Score=367, Evalue=1e-101,
Organism=Drosophila melanogaster, GI24580535, Length=369, Percent_Identity=53.3875338753388, Blast_Score=367, Evalue=1e-101,
Organism=Drosophila melanogaster, GI24580531, Length=369, Percent_Identity=53.3875338753388, Blast_Score=367, Evalue=1e-101,
Organism=Drosophila melanogaster, GI24580527, Length=369, Percent_Identity=53.1165311653117, Blast_Score=362, Evalue=1e-100,
Organism=Drosophila melanogaster, GI24580525, Length=369, Percent_Identity=53.1165311653117, Blast_Score=362, Evalue=1e-100,
Organism=Drosophila melanogaster, GI24580541, Length=368, Percent_Identity=51.0869565217391, Blast_Score=338, Evalue=3e-93,
Organism=Drosophila melanogaster, GI24580539, Length=120, Percent_Identity=51.6666666666667, Blast_Score=124, Evalue=9e-29,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636 [H]

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N [H]

EC number: =2.5.1.6 [H]

Molecular weight: Translated: 43663; Mature: 43532

Theoretical pI: Translated: 8.01; Mature: 8.01

Prosite motif: PS00376 ADOMET_SYNTHETASE_1 ; PS00377 ADOMET_SYNTHETASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSYLFTSESVSEGHPDKIADQISDAVLDAILAQDKRARVACETMVKTGVAIVAGEVTTS
CCCCEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCEEEEECCCHHH
AWIDLEALTRKVILDIGYNSSDVGFDGETCGVLNLIGKQSPDINQGVDRKNPEQQGAGDQ
HEEEHHHHHHHHEEEECCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCHHHCCCCC
GLMFGYATNETDSFMPAAIHLSHRLVEQQAKIRKKKNSALSWLRPDAKSQVTLRYEDGVA
CEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCHH
TAIDAVVLSTQHDPGVKQKDLIEAVREEILKPVLPAKWLHKGTKFHINPTGKFVIGGPVG
HHHHHHHEECCCCCCCCHHHHHHHHHHHHHHHCCCHHHHCCCCEEEECCCCCEEECCCCC
DCGLTGRKIIVDTYGGWARHGGGAFSGKDPSKVDRSAAYAARYVAKNVVAAGLADRCEVQ
CCCCCCCEEEEECCCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEE
VSYAIGVAEPTSISVTTFGTGKIADEQIEKLIRKHFDLRPFGIIQMLDLIHPMYQQTASY
EEEEEECCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCC
GHFGRKPKDFTYTDGTGAQHSATSFSWEKTDRADALRAAAKLK
CCCCCCCCCCEECCCCCCCCCCCCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SSYLFTSESVSEGHPDKIADQISDAVLDAILAQDKRARVACETMVKTGVAIVAGEVTTS
CCCEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCEEEEECCCHHH
AWIDLEALTRKVILDIGYNSSDVGFDGETCGVLNLIGKQSPDINQGVDRKNPEQQGAGDQ
HEEEHHHHHHHHEEEECCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCHHHCCCCC
GLMFGYATNETDSFMPAAIHLSHRLVEQQAKIRKKKNSALSWLRPDAKSQVTLRYEDGVA
CEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCHH
TAIDAVVLSTQHDPGVKQKDLIEAVREEILKPVLPAKWLHKGTKFHINPTGKFVIGGPVG
HHHHHHHEECCCCCCCCHHHHHHHHHHHHHHHCCCHHHHCCCCEEEECCCCCEEECCCCC
DCGLTGRKIIVDTYGGWARHGGGAFSGKDPSKVDRSAAYAARYVAKNVVAAGLADRCEVQ
CCCCCCCEEEEECCCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEE
VSYAIGVAEPTSISVTTFGTGKIADEQIEKLIRKHFDLRPFGIIQMLDLIHPMYQQTASY
EEEEEECCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCC
GHFGRKPKDFTYTDGTGAQHSATSFSWEKTDRADALRAAAKLK
CCCCCCCCCCEECCCCCCCCCCCCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA