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The map label for this gene is dosC [H]

Identifier: 78046006

GI number: 78046006

Start: 503837

End: 505666

Strand: Reverse

Name: dosC [H]

Synonym: XCV0450

Alternate gene names: 78046006

Gene position: 505666-503837 (Counterclockwise)

Preceding gene: 78046015

Following gene: 78046004

Centisome position: 9.76

GC content: 68.14

Gene sequence:

>1830_bases
ATGCACCGAATGCGGGAACTTCCGGCTGCGTTCGCCCTCATCCGGGTAACCGGTTCATCGATGCCTTGCTTCGCGCCTGG
CGACCATGCTTCGCGCCCGGCCTGCATGCCGTTGCCGCGCGCTACGGCCCGGCTTGCTCAAGCGCCCCCCTCCATGGGCC
GATATCGAGCGTTAGCACAAGACCTCTCGAGACCCTGGCGCATGGCAGACCAGCCCGAACACCCAAGCCGCCCCCGTGGC
GGGCTGCGCCGCCTGTTGCCCTGGGGCGGCACGGACACCGCGCGCCCTGCCGCTGCCCTGCCGACGGTGATCAATGGCGA
GCGCAGCGGTCATGGCCTGGCCGCGCACCAGCTGGTGCCGGCCAAGGCCAAGTCCGGCGCGCACGATCCGGCCGCTGCCA
CTGCATTGCTGATACGGCTGTTCTCGCATGCCGCGCATCCCGAAGCGCTGCTGCTCGCGTTCGCCGAAGGCATCGCCAGC
CTGCATGGCGAGCTCGGCGACATGGGCGTGCGCCTGAGCGCGGCGTACGCGGCCGGCGATTGGCCCGGCTATGGCCGTGC
CTTGCGCCAGCTCATCGACAAATACATCCGCACCATCGATATCGGCGATTCGCTGGCCGAAGGCCGCACGGAAGCCGAGC
AGCTGCGCGATCTGCTGCGCCACGCGCTGGGCAATGCCCTGGCCACGCTGTTGCAACGCAGCCCGGAGCTGGCCGAAGAG
GCGCAAACGCTGGCATCGGCGCTGCGCCACTGGCGCCCCGGACACGAGCTGATCACGCTGGAACAACGCCTGCGCGAACT
CAGCCACCAGATCGGCCTGCGCGCCGAAGATGCCAGCGAACAACAGAACCTGTTACTGGGCCTGTTCGACCTATTGCTGG
AGAACGTCGGCGAACTCCTGGACGATCGCAGCTGGTTGCAGGGCCAGATCTCGGTGGTGCGGCAACTGATTTCCGGCCCG
CTGGATGTCAGTTCCATCGAACAGGCACGCGGCACCCTGCGCGAGGTGACCTACAAGCAGGGTCTGCTCAAGCAGGGCAT
CGCCGAGTCCAAGACCGCCATGCGCGAAATGATGGTGTCCTTCGTCGACCGGCTGGACGGCATGGCCACCAGCACCGGGC
AATACCACGACCGCGTTGCCGGCTATTCGCAGGCGATCGGCGAGGCGCGCGGCATCCCCGACCTCAACCGGCTGCTGCAG
GACCTGCTGCAGGACACCGCCGGCGTGCAGGCACAGGCCCTGGCAGCGCGCGACGAACTGCTCGCGGCACGCCGGCAGGT
GGAAGATTCCGAACAGCGCATCGCCTTGCTCGAACAGGAGCTCAAGGACGTCGCCGGTTTGGTGCGCGAAGACCAACTGA
CCGGCGCGCTCAACCGGCGCGGCTTCGAAGAACTGTTCCAGCGTGAAGCCGTGCGCACCCAGCGCAGTGGCCAGCCCCTG
TGCGTGGCGATGCTGGACCTGGACGATTTCCGCCGCCTCAACGAGACCCACGGCCATGCCGGTGGCGACGCCGCGCTGCG
CCACACGGTGGAGGTGGCCAAGGCGGTGCTGCGCACCACCGATGCCATCGCCCGCTTCGGCGGCGAGGAATTCGTGCTGC
TGCTGCCGGACTCGACCATCTTCGAAGCCAGCGCGGCGGTGATCCGCCTGCAACGCGCACTGTCGCAGCGCTCGCTGCTG
CATGACGACGTGCGCGTGTTCATCAGCTTCAGCGCCGGCGTGGCGCTACGCGGGACCGACGAGACCCAGGACGAGGTCAT
CCGCCGCGCCGACCGCGCCATGTACGACGCCAAGGCGGCCGGCAAGAACCGGGTGATCAACGCCGATTAG

Upstream 100 bases:

>100_bases
TGCGACAGCCCACATTCCTTTACGCGCAGTTGCGCACGCTCAAGGCATAAGCGCCACATTGCAGTGCGATGACATCCCCG
TCTCGGTGCGTTGGCCGTAC

Downstream 100 bases:

>100_bases
CGGCGATCGTCGGCGAACGCCCGGTGCATCCGGGCGATCGCTAAGCGGTGTGTACCGTGCTGGCTGGTCTGGCGGCGCGC
CGTGCATCAATGCGCGGGCT

Product: putative signal transduction protein

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 609; Mature: 609

Protein sequence:

>609_residues
MHRMRELPAAFALIRVTGSSMPCFAPGDHASRPACMPLPRATARLAQAPPSMGRYRALAQDLSRPWRMADQPEHPSRPRG
GLRRLLPWGGTDTARPAAALPTVINGERSGHGLAAHQLVPAKAKSGAHDPAAATALLIRLFSHAAHPEALLLAFAEGIAS
LHGELGDMGVRLSAAYAAGDWPGYGRALRQLIDKYIRTIDIGDSLAEGRTEAEQLRDLLRHALGNALATLLQRSPELAEE
AQTLASALRHWRPGHELITLEQRLRELSHQIGLRAEDASEQQNLLLGLFDLLLENVGELLDDRSWLQGQISVVRQLISGP
LDVSSIEQARGTLREVTYKQGLLKQGIAESKTAMREMMVSFVDRLDGMATSTGQYHDRVAGYSQAIGEARGIPDLNRLLQ
DLLQDTAGVQAQALAARDELLAARRQVEDSEQRIALLEQELKDVAGLVREDQLTGALNRRGFEELFQREAVRTQRSGQPL
CVAMLDLDDFRRLNETHGHAGGDAALRHTVEVAKAVLRTTDAIARFGGEEFVLLLPDSTIFEASAAVIRLQRALSQRSLL
HDDVRVFISFSAGVALRGTDETQDEVIRRADRAMYDAKAAGKNRVINAD

Sequences:

>Translated_609_residues
MHRMRELPAAFALIRVTGSSMPCFAPGDHASRPACMPLPRATARLAQAPPSMGRYRALAQDLSRPWRMADQPEHPSRPRG
GLRRLLPWGGTDTARPAAALPTVINGERSGHGLAAHQLVPAKAKSGAHDPAAATALLIRLFSHAAHPEALLLAFAEGIAS
LHGELGDMGVRLSAAYAAGDWPGYGRALRQLIDKYIRTIDIGDSLAEGRTEAEQLRDLLRHALGNALATLLQRSPELAEE
AQTLASALRHWRPGHELITLEQRLRELSHQIGLRAEDASEQQNLLLGLFDLLLENVGELLDDRSWLQGQISVVRQLISGP
LDVSSIEQARGTLREVTYKQGLLKQGIAESKTAMREMMVSFVDRLDGMATSTGQYHDRVAGYSQAIGEARGIPDLNRLLQ
DLLQDTAGVQAQALAARDELLAARRQVEDSEQRIALLEQELKDVAGLVREDQLTGALNRRGFEELFQREAVRTQRSGQPL
CVAMLDLDDFRRLNETHGHAGGDAALRHTVEVAKAVLRTTDAIARFGGEEFVLLLPDSTIFEASAAVIRLQRALSQRSLL
HDDVRVFISFSAGVALRGTDETQDEVIRRADRAMYDAKAAGKNRVINAD
>Mature_609_residues
MHRMRELPAAFALIRVTGSSMPCFAPGDHASRPACMPLPRATARLAQAPPSMGRYRALAQDLSRPWRMADQPEHPSRPRG
GLRRLLPWGGTDTARPAAALPTVINGERSGHGLAAHQLVPAKAKSGAHDPAAATALLIRLFSHAAHPEALLLAFAEGIAS
LHGELGDMGVRLSAAYAAGDWPGYGRALRQLIDKYIRTIDIGDSLAEGRTEAEQLRDLLRHALGNALATLLQRSPELAEE
AQTLASALRHWRPGHELITLEQRLRELSHQIGLRAEDASEQQNLLLGLFDLLLENVGELLDDRSWLQGQISVVRQLISGP
LDVSSIEQARGTLREVTYKQGLLKQGIAESKTAMREMMVSFVDRLDGMATSTGQYHDRVAGYSQAIGEARGIPDLNRLLQ
DLLQDTAGVQAQALAARDELLAARRQVEDSEQRIALLEQELKDVAGLVREDQLTGALNRRGFEELFQREAVRTQRSGQPL
CVAMLDLDDFRRLNETHGHAGGDAALRHTVEVAKAVLRTTDAIARFGGEEFVLLLPDSTIFEASAAVIRLQRALSQRSLL
HDDVRVFISFSAGVALRGTDETQDEVIRRADRAMYDAKAAGKNRVINAD

Specific function: Overexpression leads to an increased level of c-di-GMP, which leads to changes in the cell surface, to abnormal cell division, increased biofilm formation and decreased swimming (the latter 2 in strain W3110). In a strain able to produce cellulose (strain

COG id: COG2199

COG function: function code T; FOG: GGDEF domain

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI145693134, Length=230, Percent_Identity=30.4347826086957, Blast_Score=99, Evalue=6e-22,
Organism=Escherichia coli, GI1787816, Length=161, Percent_Identity=34.1614906832298, Blast_Score=96, Evalue=8e-21,
Organism=Escherichia coli, GI1786584, Length=155, Percent_Identity=32.9032258064516, Blast_Score=87, Evalue=4e-18,
Organism=Escherichia coli, GI87081881, Length=174, Percent_Identity=34.4827586206897, Blast_Score=87, Evalue=4e-18,
Organism=Escherichia coli, GI1788381, Length=156, Percent_Identity=33.974358974359, Blast_Score=86, Evalue=8e-18,
Organism=Escherichia coli, GI87082007, Length=163, Percent_Identity=33.7423312883436, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI1787262, Length=175, Percent_Identity=31.4285714285714, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI1787541, Length=161, Percent_Identity=33.5403726708075, Blast_Score=72, Evalue=9e-14,
Organism=Escherichia coli, GI1788085, Length=181, Percent_Identity=31.4917127071823, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI87081974, Length=154, Percent_Identity=26.6233766233766, Blast_Score=63, Evalue=5e-11,
Organism=Escherichia coli, GI1787802, Length=177, Percent_Identity=24.8587570621469, Blast_Score=63, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR012292
- InterPro:   IPR009050 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 66623; Mature: 66623

Theoretical pI: Translated: 6.81; Mature: 6.81

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHRMRELPAAFALIRVTGSSMPCFAPGDHASRPACMPLPRATARLAQAPPSMGRYRALAQ
CCHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHH
DLSRPWRMADQPEHPSRPRGGLRRLLPWGGTDTARPAAALPTVINGERSGHGLAAHQLVP
HHCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCHHHCCHHHCCCCCCCCCHHHHCCC
AKAKSGAHDPAAATALLIRLFSHAAHPEALLLAFAEGIASLHGELGDMGVRLSAAYAAGD
CHHCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEHHHCCC
WPGYGRALRQLIDKYIRTIDIGDSLAEGRTEAEQLRDLLRHALGNALATLLQRSPELAEE
CCHHHHHHHHHHHHHHHHHCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
AQTLASALRHWRPGHELITLEQRLRELSHQIGLRAEDASEQQNLLLGLFDLLLENVGELL
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
DDRSWLQGQISVVRQLISGPLDVSSIEQARGTLREVTYKQGLLKQGIAESKTAMREMMVS
CCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FVDRLDGMATSTGQYHDRVAGYSQAIGEARGIPDLNRLLQDLLQDTAGVQAQALAARDEL
HHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHH
LAARRQVEDSEQRIALLEQELKDVAGLVREDQLTGALNRRGFEELFQREAVRTQRSGQPL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCE
CVAMLDLDDFRRLNETHGHAGGDAALRHTVEVAKAVLRTTDAIARFGGEEFVLLLPDSTI
EEEECCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCHH
FEASAAVIRLQRALSQRSLLHDDVRVFISFSAGVALRGTDETQDEVIRRADRAMYDAKAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCEEECCCCCHHHHHHHHHHHHHHHHHHC
GKNRVINAD
CCCCCCCCC
>Mature Secondary Structure
MHRMRELPAAFALIRVTGSSMPCFAPGDHASRPACMPLPRATARLAQAPPSMGRYRALAQ
CCHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHH
DLSRPWRMADQPEHPSRPRGGLRRLLPWGGTDTARPAAALPTVINGERSGHGLAAHQLVP
HHCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCHHHCCHHHCCCCCCCCCHHHHCCC
AKAKSGAHDPAAATALLIRLFSHAAHPEALLLAFAEGIASLHGELGDMGVRLSAAYAAGD
CHHCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEHHHCCC
WPGYGRALRQLIDKYIRTIDIGDSLAEGRTEAEQLRDLLRHALGNALATLLQRSPELAEE
CCHHHHHHHHHHHHHHHHHCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
AQTLASALRHWRPGHELITLEQRLRELSHQIGLRAEDASEQQNLLLGLFDLLLENVGELL
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
DDRSWLQGQISVVRQLISGPLDVSSIEQARGTLREVTYKQGLLKQGIAESKTAMREMMVS
CCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FVDRLDGMATSTGQYHDRVAGYSQAIGEARGIPDLNRLLQDLLQDTAGVQAQALAARDEL
HHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHH
LAARRQVEDSEQRIALLEQELKDVAGLVREDQLTGALNRRGFEELFQREAVRTQRSGQPL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCE
CVAMLDLDDFRRLNETHGHAGGDAALRHTVEVAKAVLRTTDAIARFGGEEFVLLLPDSTI
EEEECCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCHH
FEASAAVIRLQRALSQRSLLHDDVRVFISFSAGVALRGTDETQDEVIRRADRAMYDAKAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCEEECCCCCHHHHHHHHHHHHHHHHHHC
GKNRVINAD
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]