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The map label for this gene is vanB [H]

Identifier: 78045875

GI number: 78045875

Start: 366404

End: 367363

Strand: Reverse

Name: vanB [H]

Synonym: XCV0319

Alternate gene names: 78045875

Gene position: 367363-366404 (Counterclockwise)

Preceding gene: 78045876

Following gene: 78045874

Centisome position: 7.09

GC content: 68.12

Gene sequence:

>960_bases
ATGAGCCTGCATGAAGTGCGCGTTGCCGAGGTCGTCGATCAGGGTCACCGCCAGCGCGCGATCCGGCTGGAACCGGTGGG
CGCGCAATTGCCGGCCTTCGAGGCCGGCGCGCATGTCGACCTGCATCTGCCGGATGGCTTGGTTCGCCAGTATTCGATCG
CCAGCGCGCCGCATGTGCGCGACCACTATCTGCTGTGCGTCAAGCTGGCAGACGCCTCTCGTGGCGGCTCACGGCATCTG
TGCGAACAGCTCGCAACCGGCGACCGCCTGCGCATCTCCACACCGCGCAATCTGTTTCCGCTGCACCCCGGCGAGCGCCA
TGTGCTGCTGGCGGCCGGCATCGGCATCACCCCGCTGCTGTCGATGGCCGAGGCGCTGGAAGCGCGCGGCGAGGCCTTCG
TGCTGCACTATTACGCGCGCCGGCATGCGGATGTGGCGTTTTCGCAGCGGCTGCAACAAGGCTTTACGCATGGGCAGGTG
TACCTGCACCTGAGCGATGGCGGGCAGAGTCCACGCGTGCATGTGCCCGAGGAACTGCGCCAGGCACGTGCGCGCGACCA
GCTGTATCTGTGCGGGCCGGCGGCGTTCATGGACCACTTCACCGTGCTGGCAAAGGCGCATGGCTGGACGGCGGCGCAAC
TGCATCGCGAACACTTCGCCGCCGCCGCGCCCAGCCTCGCCCACGATGCCGACAGCGCGTTCGAGGTGGAACTGGCTGCC
AGCGGCCGCGTGGTGCAGGTGCCGGCCGATTGCAGCATCGCCAGCGCCCTGCGCGATGCCGGTATCGAGGTGCCGCTGTC
GTGCGAACAAGGCATGTGCGGCGCCTGTCTGACCGGCGTGCTGGCTGGCACGCCAGATCACCGCGACAGCGTGCTCAGCG
ACGCCGAACGCGCCGCCAACCGGCAGATCACCGTGTGCTGTTCGCGCAGCCGCTCGCCACGGTTGGTGCTGGATCTTTAA

Upstream 100 bases:

>100_bases
TGCACATTCCCGCCGATCGCAGTTCCATCGCCTATCGCCGCGGGCTGCGCAGCCAGGGCTATAGCGACTTCTTCCTGCGC
TGACGGCCGAGGACACCCGC

Downstream 100 bases:

>100_bases
GCTCGGTCGTCGCGGCGCCATTGCGGTTGTCATTCACCTCGCGCAGCCTGACGCCTCGCTTGCCCTTCACGATGGCAGGC
GACACAGCGCGCCATCGCCC

Product: putative vanillate O-demethylase oxidoreductase

Products: 3,4-dihydroxybenzoate; NAD+; H2O; formaldehyde

Alternate protein names: Vanillate degradation ferredoxin-like protein [H]

Number of amino acids: Translated: 319; Mature: 318

Protein sequence:

>319_residues
MSLHEVRVAEVVDQGHRQRAIRLEPVGAQLPAFEAGAHVDLHLPDGLVRQYSIASAPHVRDHYLLCVKLADASRGGSRHL
CEQLATGDRLRISTPRNLFPLHPGERHVLLAAGIGITPLLSMAEALEARGEAFVLHYYARRHADVAFSQRLQQGFTHGQV
YLHLSDGGQSPRVHVPEELRQARARDQLYLCGPAAFMDHFTVLAKAHGWTAAQLHREHFAAAAPSLAHDADSAFEVELAA
SGRVVQVPADCSIASALRDAGIEVPLSCEQGMCGACLTGVLAGTPDHRDSVLSDAERAANRQITVCCSRSRSPRLVLDL

Sequences:

>Translated_319_residues
MSLHEVRVAEVVDQGHRQRAIRLEPVGAQLPAFEAGAHVDLHLPDGLVRQYSIASAPHVRDHYLLCVKLADASRGGSRHL
CEQLATGDRLRISTPRNLFPLHPGERHVLLAAGIGITPLLSMAEALEARGEAFVLHYYARRHADVAFSQRLQQGFTHGQV
YLHLSDGGQSPRVHVPEELRQARARDQLYLCGPAAFMDHFTVLAKAHGWTAAQLHREHFAAAAPSLAHDADSAFEVELAA
SGRVVQVPADCSIASALRDAGIEVPLSCEQGMCGACLTGVLAGTPDHRDSVLSDAERAANRQITVCCSRSRSPRLVLDL
>Mature_318_residues
SLHEVRVAEVVDQGHRQRAIRLEPVGAQLPAFEAGAHVDLHLPDGLVRQYSIASAPHVRDHYLLCVKLADASRGGSRHLC
EQLATGDRLRISTPRNLFPLHPGERHVLLAAGIGITPLLSMAEALEARGEAFVLHYYARRHADVAFSQRLQQGFTHGQVY
LHLSDGGQSPRVHVPEELRQARARDQLYLCGPAAFMDHFTVLAKAHGWTAAQLHREHFAAAAPSLAHDADSAFEVELAAS
GRVVQVPADCSIASALRDAGIEVPLSCEQGMCGACLTGVLAGTPDHRDSVLSDAERAANRQITVCCSRSRSPRLVLDL

Specific function: Unknown

COG id: COG1018

COG function: function code C; Flavodoxin reductases (ferredoxin-NADPH reductases) family 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding FR-type domain [H]

Homologues:

Organism=Escherichia coli, GI1788104, Length=323, Percent_Identity=37.1517027863777, Blast_Score=186, Evalue=2e-48,
Organism=Escherichia coli, GI1787098, Length=278, Percent_Identity=27.6978417266187, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1787658, Length=288, Percent_Identity=27.7777777777778, Blast_Score=66, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006058
- InterPro:   IPR012675
- InterPro:   IPR017927
- InterPro:   IPR001041
- InterPro:   IPR001433
- InterPro:   IPR000951
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00111 Fer2; PF00175 NAD_binding_1 [H]

EC number: 1.14.13.82

Molecular weight: Translated: 34593; Mature: 34461

Theoretical pI: Translated: 7.06; Mature: 7.06

Prosite motif: PS00197 2FE2S_FER_1 ; PS51085 2FE2S_FER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLHEVRVAEVVDQGHRQRAIRLEPVGAQLPAFEAGAHVDLHLPDGLVRQYSIASAPHVR
CCCHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCEEEEECCCCHHHHHHCCCCCCCC
DHYLLCVKLADASRGGSRHLCEQLATGDRLRISTPRNLFPLHPGERHVLLAAGIGITPLL
CCEEEEEEEECCCCCCHHHHHHHHCCCCEEEECCCCCCCCCCCCCCEEEEEECCCHHHHH
SMAEALEARGEAFVLHYYARRHADVAFSQRLQQGFTHGQVYLHLSDGGQSPRVHVPEELR
HHHHHHHHCCCEEEEEEHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCEECCHHHH
QARARDQLYLCGPAAFMDHFTVLAKAHGWTAAQLHREHFAAAAPSLAHDADSAFEVELAA
HHHCCCCEEEECHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHCCCCCCEEEEEEC
SGRVVQVPADCSIASALRDAGIEVPLSCEQGMCGACLTGVLAGTPDHRDSVLSDAERAAN
CCCEEEECCCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHCC
RQITVCCSRSRSPRLVLDL
CEEEEEECCCCCCEEEEEC
>Mature Secondary Structure 
SLHEVRVAEVVDQGHRQRAIRLEPVGAQLPAFEAGAHVDLHLPDGLVRQYSIASAPHVR
CCHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCEEEEECCCCHHHHHHCCCCCCCC
DHYLLCVKLADASRGGSRHLCEQLATGDRLRISTPRNLFPLHPGERHVLLAAGIGITPLL
CCEEEEEEEECCCCCCHHHHHHHHCCCCEEEECCCCCCCCCCCCCCEEEEEECCCHHHHH
SMAEALEARGEAFVLHYYARRHADVAFSQRLQQGFTHGQVYLHLSDGGQSPRVHVPEELR
HHHHHHHHCCCEEEEEEHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCEECCHHHH
QARARDQLYLCGPAAFMDHFTVLAKAHGWTAAQLHREHFAAAAPSLAHDADSAFEVELAA
HHHCCCCEEEECHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHCCCCCCEEEEEEC
SGRVVQVPADCSIASALRDAGIEVPLSCEQGMCGACLTGVLAGTPDHRDSVLSDAERAAN
CCCEEEECCCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHCC
RQITVCCSRSRSPRLVLDL
CEEEEEECCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: vanillate; O2; NADH; H+

Specific reaction: vanillate + O2 + NADH + H+ = 3,4-dihydroxybenzoate + NAD+ + H2O + formaldehyde

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9098058 [H]