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The map label for this gene is gatA [H]

Identifier: 78045871

GI number: 78045871

Start: 359853

End: 361313

Strand: Reverse

Name: gatA [H]

Synonym: XCV0315

Alternate gene names: 78045871

Gene position: 361313-359853 (Counterclockwise)

Preceding gene: 78045872

Following gene: 78045870

Centisome position: 6.98

GC content: 72.07

Gene sequence:

>1461_bases
ATGCAACTCCCCGCGCAATGGCAGGCAGGCGTCGATCGCAATACCGAAGTGCTGCGTGGGCACGCGCAGCTGCTGGCCAG
CTTCCATGCGCCGCCTGCAACGGCACACGAGGGCGGCGGTGCGCGCGGCCAGATTGCCTCCATCGTCAGCACCACGCGCA
GCCAGCCCACGCTGGCCGCGCGACTGGCCACCCAGACGCTGACGCGCATCAACCAGGCCAACGACCGCCTGTGCGCCATC
ACCCGCCTGCTGCCGGCGCGTGCCGCGGCCGATGCCGCGCGCGTGCAGGCCGCGCTTGCCGGGGGCAGCGACGGGGGCGC
GTTGGCCGGCGTGCCATTCGTGGTCAAGGACCTGTTCGACGTCGCCGGGCAGGTCACCACCGCCGGCGCCGCGGTGCGTG
CGCACTGCCCGCCGGCCAGCCGCGATGCGGCGGTGGTGCAACGGCTCAGCGACGCCGGGGCCGTGCTGGTGGGTACCGCC
AACATGGACGAATTCGCGTACGGTTTTGCCACCGTCAACGCGCATTACGGCACCACCGCCAATCCGCACGATCTCCAGCA
CCTGGCGGGCGGCTCGTCGGGCGGCTCGGCGGCTGCGGTGGCGGCCGGGCTGGTGCCGTTCGCGCTTGGCTCGGACACCA
ATGGTTCCATCCGCGTGCCGGCCGCGCTGTGCGGCATCTACGGCCTGCGCCCCACGCACGGCGCCGTGCCCCTGGATGGC
GTGTTCCCGTTTGTCGACGCACTGGACGTGGCCGGCCCATTCGCCACCTCGGTGGCCGACCTGCGCCGCATCCATGAGGT
ACTGCGCGGGCAGCCGCTGCCTTCGTGCCATGCCGGCAACCTGCGTGTCGCGCGGTTGGGCGGCTGGTTTGAACGCAATC
TGGATCCGGATCTGGAGGCCGGGCTGCAGGCCCTGCTCACCGCCTGCAACAGCCTTTCGTCGATCGACCTGCCGGAGGCC
GAACGCGCACGCGCCGCTGCCTTCGTCCTGACCGCTGCCGAAGGCGGGCATCGTCACCGCAGCGCACTCACCGCGCACGG
CGCGCAGTTCGACCCGGCCACCCGCGACCGCCTGCTCGCCGGCTTGCAGCTGCCCGCCAGCGCGGTGGCCGATGCGCACC
GCTTCGCACAATGGTTTGCCGATGCCATGCACGCGCTGTGGGACCAGGTGGAGGTGCTCATCGCGCCGGCCACCCCGTGC
GTGGCGCCACGCATCGACCAGGACACCATCCAGATCGACGGTTTGCCCGTCTCCGCACGCGCGAACCTGGGCATTTTTAC
CCAGCCACTCGGCCTGGCCGCCTGCCCGGTGCTGGCCGCACCGTTGCCGCGTCCCGGGCGCCTGCCACTGGGCGTGCAAC
TGATCGCCGCGCCCGGCCGTGAAGACCGCCTGTTTGCGCTGGCCGCGCAACTCGAACGCGACGGCCTGCTCGCCTTCAGC
CCGCCGCCGGAGTCACGCTGA

Upstream 100 bases:

>100_bases
GTCGGCTCTCAACGCATCAGCCAGCACGTTTGCACGACCCGCACGCCAAGCACACAAGCGAATCTCAACTCCCCAATCCC
CGCTTTCCAACAGGTGATCC

Downstream 100 bases:

>100_bases
TGGTGCATACCCTGCGCGCGCGCCGCGGCATGGTGGTCGCACCGCACCACTTGGCCGCACAGGCCGGGCGCGATGTCTTG
CGCGACGGCGGCAATGCCGT

Product: amidase

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 486; Mature: 486

Protein sequence:

>486_residues
MQLPAQWQAGVDRNTEVLRGHAQLLASFHAPPATAHEGGGARGQIASIVSTTRSQPTLAARLATQTLTRINQANDRLCAI
TRLLPARAAADAARVQAALAGGSDGGALAGVPFVVKDLFDVAGQVTTAGAAVRAHCPPASRDAAVVQRLSDAGAVLVGTA
NMDEFAYGFATVNAHYGTTANPHDLQHLAGGSSGGSAAAVAAGLVPFALGSDTNGSIRVPAALCGIYGLRPTHGAVPLDG
VFPFVDALDVAGPFATSVADLRRIHEVLRGQPLPSCHAGNLRVARLGGWFERNLDPDLEAGLQALLTACNSLSSIDLPEA
ERARAAAFVLTAAEGGHRHRSALTAHGAQFDPATRDRLLAGLQLPASAVADAHRFAQWFADAMHALWDQVEVLIAPATPC
VAPRIDQDTIQIDGLPVSARANLGIFTQPLGLAACPVLAAPLPRPGRLPLGVQLIAAPGREDRLFALAAQLERDGLLAFS
PPPESR

Sequences:

>Translated_486_residues
MQLPAQWQAGVDRNTEVLRGHAQLLASFHAPPATAHEGGGARGQIASIVSTTRSQPTLAARLATQTLTRINQANDRLCAI
TRLLPARAAADAARVQAALAGGSDGGALAGVPFVVKDLFDVAGQVTTAGAAVRAHCPPASRDAAVVQRLSDAGAVLVGTA
NMDEFAYGFATVNAHYGTTANPHDLQHLAGGSSGGSAAAVAAGLVPFALGSDTNGSIRVPAALCGIYGLRPTHGAVPLDG
VFPFVDALDVAGPFATSVADLRRIHEVLRGQPLPSCHAGNLRVARLGGWFERNLDPDLEAGLQALLTACNSLSSIDLPEA
ERARAAAFVLTAAEGGHRHRSALTAHGAQFDPATRDRLLAGLQLPASAVADAHRFAQWFADAMHALWDQVEVLIAPATPC
VAPRIDQDTIQIDGLPVSARANLGIFTQPLGLAACPVLAAPLPRPGRLPLGVQLIAAPGREDRLFALAAQLERDGLLAFS
PPPESR
>Mature_486_residues
MQLPAQWQAGVDRNTEVLRGHAQLLASFHAPPATAHEGGGARGQIASIVSTTRSQPTLAARLATQTLTRINQANDRLCAI
TRLLPARAAADAARVQAALAGGSDGGALAGVPFVVKDLFDVAGQVTTAGAAVRAHCPPASRDAAVVQRLSDAGAVLVGTA
NMDEFAYGFATVNAHYGTTANPHDLQHLAGGSSGGSAAAVAAGLVPFALGSDTNGSIRVPAALCGIYGLRPTHGAVPLDG
VFPFVDALDVAGPFATSVADLRRIHEVLRGQPLPSCHAGNLRVARLGGWFERNLDPDLEAGLQALLTACNSLSSIDLPEA
ERARAAAFVLTAAEGGHRHRSALTAHGAQFDPATRDRLLAGLQLPASAVADAHRFAQWFADAMHALWDQVEVLIAPATPC
VAPRIDQDTIQIDGLPVSARANLGIFTQPLGLAACPVLAAPLPRPGRLPLGVQLIAAPGREDRLFALAAQLERDGLLAFS
PPPESR

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI195972892, Length=485, Percent_Identity=26.1855670103093, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI222831590, Length=455, Percent_Identity=27.2527472527473, Blast_Score=112, Evalue=1e-24,
Organism=Homo sapiens, GI166795287, Length=149, Percent_Identity=35.5704697986577, Blast_Score=67, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI17543272, Length=420, Percent_Identity=28.5714285714286, Blast_Score=120, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI17537465, Length=475, Percent_Identity=26.5263157894737, Blast_Score=113, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17556264, Length=181, Percent_Identity=34.2541436464088, Blast_Score=94, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17538252, Length=166, Percent_Identity=31.9277108433735, Blast_Score=79, Evalue=8e-15,
Organism=Caenorhabditis elegans, GI17556276, Length=162, Percent_Identity=38.2716049382716, Blast_Score=78, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17556278, Length=162, Percent_Identity=38.2716049382716, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI71990152, Length=155, Percent_Identity=34.8387096774194, Blast_Score=76, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6319685, Length=382, Percent_Identity=31.6753926701571, Blast_Score=117, Evalue=3e-27,
Organism=Saccharomyces cerevisiae, GI6323950, Length=390, Percent_Identity=24.3589743589744, Blast_Score=87, Evalue=5e-18,
Organism=Saccharomyces cerevisiae, GI6320448, Length=160, Percent_Identity=33.125, Blast_Score=68, Evalue=4e-12,
Organism=Drosophila melanogaster, GI24648113, Length=473, Percent_Identity=31.9238900634249, Blast_Score=116, Evalue=3e-26,
Organism=Drosophila melanogaster, GI21356731, Length=163, Percent_Identity=40.4907975460123, Blast_Score=111, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24652985, Length=479, Percent_Identity=24.2171189979123, Blast_Score=101, Evalue=1e-21,
Organism=Drosophila melanogaster, GI19922090, Length=479, Percent_Identity=24.2171189979123, Blast_Score=101, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24652981, Length=479, Percent_Identity=24.2171189979123, Blast_Score=101, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24652983, Length=479, Percent_Identity=24.2171189979123, Blast_Score=101, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24644968, Length=246, Percent_Identity=31.3008130081301, Blast_Score=99, Evalue=6e-21,
Organism=Drosophila melanogaster, GI24648435, Length=201, Percent_Identity=31.3432835820896, Blast_Score=94, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24648437, Length=201, Percent_Identity=31.3432835820896, Blast_Score=94, Evalue=3e-19,
Organism=Drosophila melanogaster, GI45550774, Length=201, Percent_Identity=31.3432835820896, Blast_Score=94, Evalue=3e-19,
Organism=Drosophila melanogaster, GI161078093, Length=413, Percent_Identity=25.4237288135593, Blast_Score=90, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24648441, Length=139, Percent_Identity=38.1294964028777, Blast_Score=89, Evalue=9e-18,
Organism=Drosophila melanogaster, GI24648439, Length=139, Percent_Identity=38.1294964028777, Blast_Score=89, Evalue=9e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 6.3.5.-

Molecular weight: Translated: 50178; Mature: 50178

Theoretical pI: Translated: 6.92; Mature: 6.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQLPAQWQAGVDRNTEVLRGHAQLLASFHAPPATAHEGGGARGQIASIVSTTRSQPTLAA
CCCCCCHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCCCHHHH
RLATQTLTRINQANDRLCAITRLLPARAAADAARVQAALAGGSDGGALAGVPFVVKDLFD
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHH
VAGQVTTAGAAVRAHCPPASRDAAVVQRLSDAGAVLVGTANMDEFAYGFATVNAHYGTTA
HHCCCCCCCCEEEECCCCCCCHHHHHHHHHCCCEEEEECCCCHHHHHEEEEEEECCCCCC
NPHDLQHLAGGSSGGSAAAVAAGLVPFALGSDTNGSIRVPAALCGIYGLRPTHGAVPLDG
CHHHHHHHCCCCCCCCHHHHHHHHHHHEECCCCCCCEECHHHHHHHHCCCCCCCCCCCCH
VFPFVDALDVAGPFATSVADLRRIHEVLRGQPLPSCHAGNLRVARLGGWFERNLDPDLEA
HHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCHHHCCCCCCHHH
GLQALLTACNSLSSIDLPEAERARAAAFVLTAAEGGHRHRSALTAHGAQFDPATRDRLLA
HHHHHHHHHHCCCCCCCCCHHHHHHEEEEEEECCCCCHHHHHHHHCCCCCCCHHHHHHHH
GLQLPASAVADAHRFAQWFADAMHALWDQVEVLIAPATPCVAPRIDQDTIQIDGLPVSAR
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCCEEEECCEEEECC
ANLGIFTQPLGLAACPVLAAPLPRPGRLPLGVQLIAAPGREDRLFALAAQLERDGLLAFS
CCCCEEECCCHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHCCEEEEC
PPPESR
CCCCCC
>Mature Secondary Structure
MQLPAQWQAGVDRNTEVLRGHAQLLASFHAPPATAHEGGGARGQIASIVSTTRSQPTLAA
CCCCCCHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCCCHHHH
RLATQTLTRINQANDRLCAITRLLPARAAADAARVQAALAGGSDGGALAGVPFVVKDLFD
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHH
VAGQVTTAGAAVRAHCPPASRDAAVVQRLSDAGAVLVGTANMDEFAYGFATVNAHYGTTA
HHCCCCCCCCEEEECCCCCCCHHHHHHHHHCCCEEEEECCCCHHHHHEEEEEEECCCCCC
NPHDLQHLAGGSSGGSAAAVAAGLVPFALGSDTNGSIRVPAALCGIYGLRPTHGAVPLDG
CHHHHHHHCCCCCCCCHHHHHHHHHHHEECCCCCCCEECHHHHHHHHCCCCCCCCCCCCH
VFPFVDALDVAGPFATSVADLRRIHEVLRGQPLPSCHAGNLRVARLGGWFERNLDPDLEA
HHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCHHHCCCCCCHHH
GLQALLTACNSLSSIDLPEAERARAAAFVLTAAEGGHRHRSALTAHGAQFDPATRDRLLA
HHHHHHHHHHCCCCCCCCCHHHHHHEEEEEEECCCCCHHHHHHHHCCCCCCCHHHHHHHH
GLQLPASAVADAHRFAQWFADAMHALWDQVEVLIAPATPCVAPRIDQDTIQIDGLPVSAR
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCCEEEECCEEEECC
ANLGIFTQPLGLAACPVLAAPLPRPGRLPLGVQLIAAPGREDRLFALAAQLERDGLLAFS
CCCCEEECCCHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHCCEEEEC
PPPESR
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA