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The map label for this gene is lexA

Identifier: 78045221

GI number: 78045221

Start: 1235214

End: 1235828

Strand: Direct

Name: lexA

Synonym: CHY_1378

Alternate gene names: 78045221

Gene position: 1235214-1235828 (Clockwise)

Preceding gene: 78044888

Following gene: 78044631

Centisome position: 51.43

GC content: 38.7

Gene sequence:

>615_bases
GTGAAAAACTCCCTTCTTTCGCACCAGGAAAAAAAAATAATCTCATTTATAAAGCAATACCTCAATGAGAATGGATATCC
TCCCACAATTCGAGAAATCTGTCAGGGTGTTGGATTAAGCTCTCCCTCCACTGTTCATCACCACTTGAAAAACTTGGAAA
GCAAAGGCTATCTGCAACGAAATCCAACCAAACCACGAGCCTTAGAACTTGTAGCCGAAAAAGCACCGGAAGAGCTTTTA
TCAATTCCCTTATTGGGCAATGTTGCTGCCGGTTATCCAACTTTAGCCATTGAAAATGCCGAAGAAGAAATGCAAATACC
CAAGTCCCTATTTCCGGAAAAAGAGTTATTTGCTTTGCGCATTAAGGGAGACAGTATGATCGAGGAAGGAATACTGCCAG
GAGATGTGATAATTGTAAAAAAGCAAGAAGTAGCCGAAAACGGTGACATCGTTGTCGCTTATTTAGAGGGAGAAGTTACT
GTAAAAAAATTCTGGAAAGATAGTGTGAATGGAGTGATTAAATTAATTCCAGCAAATTCAAAATACGAACCGATTATCAT
AAACCGGGAAACAAAAATATTGGGTAAAGTTATAGGCTTGTTAAGACGATATTAA

Upstream 100 bases:

>100_bases
TATAAGTTTTTCCTGCAAAATAATTTCGAACTTATGTTTTCAAAAACCTAAAACATTATTATAATAAACTTAAAAGCTAC
CAAAATGAGGTGATTTTTGC

Downstream 100 bases:

>100_bases
ATAAGCCAGCAACTTTTGCTGGCTTATTTCCATATTTTTAAAGCTTCCTTTAACAAATCCTGGTGCAGTTCCGCAGGTAG
TTCCAGCCAAAATACTTTAT

Product: LexA repressor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 204; Mature: 204

Protein sequence:

>204_residues
MKNSLLSHQEKKIISFIKQYLNENGYPPTIREICQGVGLSSPSTVHHHLKNLESKGYLQRNPTKPRALELVAEKAPEELL
SIPLLGNVAAGYPTLAIENAEEEMQIPKSLFPEKELFALRIKGDSMIEEGILPGDVIIVKKQEVAENGDIVVAYLEGEVT
VKKFWKDSVNGVIKLIPANSKYEPIIINRETKILGKVIGLLRRY

Sequences:

>Translated_204_residues
MKNSLLSHQEKKIISFIKQYLNENGYPPTIREICQGVGLSSPSTVHHHLKNLESKGYLQRNPTKPRALELVAEKAPEELL
SIPLLGNVAAGYPTLAIENAEEEMQIPKSLFPEKELFALRIKGDSMIEEGILPGDVIIVKKQEVAENGDIVVAYLEGEVT
VKKFWKDSVNGVIKLIPANSKYEPIIINRETKILGKVIGLLRRY
>Mature_204_residues
MKNSLLSHQEKKIISFIKQYLNENGYPPTIREICQGVGLSSPSTVHHHLKNLESKGYLQRNPTKPRALELVAEKAPEELL
SIPLLGNVAAGYPTLAIENAEEEMQIPKSLFPEKELFALRIKGDSMIEEGILPGDVIIVKKQEVAENGDIVVAYLEGEVT
VKKFWKDSVNGVIKLIPANSKYEPIIINRETKILGKVIGLLRRY

Specific function: Represses a number of genes involved in the response to DNA damage (SOS response), including recA and lexA. In the presence of single-stranded DNA, recA interacts with lexA causing an autocatalytic cleavage which disrupts the DNA-binding part of lexA, lea

COG id: COG1974

COG function: function code KT; SOS-response transcriptional repressors (RecA-mediated autopeptidases)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S24 family

Homologues:

Organism=Escherichia coli, GI1790476, Length=200, Percent_Identity=35.5, Blast_Score=123, Evalue=1e-29,
Organism=Escherichia coli, GI1787431, Length=131, Percent_Identity=35.8778625954198, Blast_Score=77, Evalue=9e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LEXA_CARHZ (Q3ACC4)

Other databases:

- EMBL:   CP000141
- RefSeq:   YP_360210.1
- ProteinModelPortal:   Q3ACC4
- SMR:   Q3ACC4
- STRING:   Q3ACC4
- MEROPS:   S24.001
- GeneID:   3728347
- GenomeReviews:   CP000141_GR
- KEGG:   chy:CHY_1378
- NMPDR:   fig|246194.3.peg.1430
- TIGR:   CHY_1378
- eggNOG:   COG1974
- HOGENOM:   HBG679610
- OMA:   VIAMTDE
- BioCyc:   CHYD246194:CHY_1378-MONOMER
- GO:   GO:0006508
- GO:   GO:0006350
- HAMAP:   MF_00015
- InterPro:   IPR006199
- InterPro:   IPR006200
- InterPro:   IPR006197
- InterPro:   IPR019759
- InterPro:   IPR015927
- InterPro:   IPR011056
- InterPro:   IPR011991
- Gene3D:   G3DSA:2.10.109.10
- Gene3D:   G3DSA:1.10.10.10
- PRINTS:   PR00726
- TIGRFAMs:   TIGR00498

Pfam domain/function: PF01726 LexA_DNA_bind; PF00717 Peptidase_S24; SSF51306 Pept_S24_S26_C

EC number: =3.4.21.88

Molecular weight: Translated: 22824; Mature: 22824

Theoretical pI: Translated: 8.40; Mature: 8.40

Prosite motif: NA

Important sites: ACT_SITE 125-125 ACT_SITE 162-162

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNSLLSHQEKKIISFIKQYLNENGYPPTIREICQGVGLSSPSTVHHHLKNLESKGYLQR
CCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCC
NPTKPRALELVAEKAPEELLSIPLLGNVAAGYPTLAIENAEEEMQIPKSLFPEKELFALR
CCCCHHHHHHHHHHCCHHHHHCCCCCCCCCCCCEEEEECCHHHHHCCHHHCCCCCEEEEE
IKGDSMIEEGILPGDVIIVKKQEVAENGDIVVAYLEGEVTVKKFWKDSVNGVIKLIPANS
ECCCHHHHHCCCCCCEEEEECHHHCCCCCEEEEEECCCCHHHHHHHHCCCCEEEEEECCC
KYEPIIINRETKILGKVIGLLRRY
CCCCEEEECHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKNSLLSHQEKKIISFIKQYLNENGYPPTIREICQGVGLSSPSTVHHHLKNLESKGYLQR
CCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCC
NPTKPRALELVAEKAPEELLSIPLLGNVAAGYPTLAIENAEEEMQIPKSLFPEKELFALR
CCCCHHHHHHHHHHCCHHHHHCCCCCCCCCCCCEEEEECCHHHHHCCHHHCCCCCEEEEE
IKGDSMIEEGILPGDVIIVKKQEVAENGDIVVAYLEGEVTVKKFWKDSVNGVIKLIPANS
ECCCHHHHHCCCCCCEEEEECHHHCCCCCEEEEEECCCCHHHHHHHHCCCCEEEEEECCC
KYEPIIINRETKILGKVIGLLRRY
CCCCEEEECHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA