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The map label for this gene is glnB [H]

Identifier: 78042932

GI number: 78042932

Start: 61861

End: 62223

Strand: Direct

Name: glnB [H]

Synonym: CHY_0072

Alternate gene names: 78042932

Gene position: 61861-62223 (Clockwise)

Preceding gene: 78043490

Following gene: 78044978

Centisome position: 2.58

GC content: 43.53

Gene sequence:

>363_bases
ATGAAGAAAATTGAAGCGTTAATAAGGCCCCAGAAATTAGGGGAAGTGAAAGAGGCTCTTAATAAATTGGGTATTTCGGG
TATGACGGTAACAGAAGTGGTGGGCTGCGGGCGGCAAAAAGGGCAGAAGGAAATTTACCGGGGTATTGAGTACGAGATTA
ATCTTTTGCCCAAGGTGAAGATTGAAATTGTTACTGTTGATGAAAAGGTGGAGGAAATTATTAAGATAATTTCCGAGAGT
GCCCGAACGGGTGCAATTGGTGATGGCAAGATTTTTATTAGTGAGGTTTTAGATGCGGTCCGAATTAGAACCGGTGAACG
GGGGACAGCAGCTATAACGTCACCAAAGGAGGGAGAAAGATGA

Upstream 100 bases:

>100_bases
GTTCCAAAAGGCTTTTTAGCTTTTATAGGCAATGAAGCCTCTGGTGAAGGGTCCGGCGCCGCCCTTTATCAGGGGCTAAA
TTTTTTAGGAGGTGGTTTTC

Downstream 100 bases:

>100_bases
AGAAGAAATTTTTATCCTTGCTTTTTGGCCTAATCCTTTTACCTAATTTATCTTTAGCCGGAGAGCCAGGGTATAACCCG
GGAGATGTTACCTTTGTACT

Product: nitrogen regulatory protein P-II

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 120; Mature: 120

Protein sequence:

>120_residues
MKKIEALIRPQKLGEVKEALNKLGISGMTVTEVVGCGRQKGQKEIYRGIEYEINLLPKVKIEIVTVDEKVEEIIKIISES
ARTGAIGDGKIFISEVLDAVRIRTGERGTAAITSPKEGER

Sequences:

>Translated_120_residues
MKKIEALIRPQKLGEVKEALNKLGISGMTVTEVVGCGRQKGQKEIYRGIEYEINLLPKVKIEIVTVDEKVEEIIKIISES
ARTGAIGDGKIFISEVLDAVRIRTGERGTAAITSPKEGER
>Mature_120_residues
MKKIEALIRPQKLGEVKEALNKLGISGMTVTEVVGCGRQKGQKEIYRGIEYEINLLPKVKIEIVTVDEKVEEIIKIISES
ARTGAIGDGKIFISEVLDAVRIRTGERGTAAITSPKEGER

Specific function: In nitrogen-limiting conditions, when the ratio of Gln to 2-ketoglutarate decreases, P-II is uridylylated to P-II-UMP. P- II-UMP allows the deadenylation of glutamine synthetase (GS), thus activating the enzyme. Conversely, in nitrogen excess P-II is deur

COG id: COG0347

COG function: function code E; Nitrogen regulatory protein PII

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the P(II) protein family [H]

Homologues:

Organism=Escherichia coli, GI1788904, Length=112, Percent_Identity=58.9285714285714, Blast_Score=131, Evalue=1e-32,
Organism=Escherichia coli, GI1786655, Length=112, Percent_Identity=54.4642857142857, Blast_Score=130, Evalue=2e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002187
- InterPro:   IPR011322
- InterPro:   IPR015867
- InterPro:   IPR017918 [H]

Pfam domain/function: PF00543 P-II [H]

EC number: NA

Molecular weight: Translated: 13178; Mature: 13178

Theoretical pI: Translated: 9.10; Mature: 9.10

Prosite motif: PS00638 PII_GLNB_CTER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKIEALIRPQKLGEVKEALNKLGISGMTVTEVVGCGRQKGQKEIYRGIEYEINLLPKVK
CCHHHHHHCCHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCHHHHHCCCCEEEEECCCEE
IEIVTVDEKVEEIIKIISESARTGAIGDGKIFISEVLDAVRIRTGERGTAAITSPKEGER
EEEEECCHHHHHHHHHHHHCCCCCCCCCCEEHHHHHHHHHHHCCCCCCCEEECCCCCCCC
>Mature Secondary Structure
MKKIEALIRPQKLGEVKEALNKLGISGMTVTEVVGCGRQKGQKEIYRGIEYEINLLPKVK
CCHHHHHHCCHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCHHHHHCCCCEEEEECCCEE
IEIVTVDEKVEEIIKIISESARTGAIGDGKIFISEVLDAVRIRTGERGTAAITSPKEGER
EEEEECCHHHHHHHHHHHHCCCCCCCCCCEEHHHHHHHHHHHCCCCCCCEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]