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The map label for this gene is pilT [H]

Identifier: 78044094

GI number: 78044094

Start: 546099

End: 547187

Strand: Direct

Name: pilT [H]

Synonym: CHY_0631

Alternate gene names: 78044094

Gene position: 546099-547187 (Clockwise)

Preceding gene: 78044711

Following gene: 78043922

Centisome position: 22.74

GC content: 49.22

Gene sequence:

>1089_bases
ATGATTAATGAGCTTTTACGGTTAATGGTAGAGTTTAAAGCGTCGGACTTGCATTTAACGGTGAACTTTCCCCCGGCTTA
CCGGATTCACGGCAATATCCGGCCGCTAACGGAGCTTGCCAGGGAAAATCCGGGGATACCGCAAAGCTTGACAAGGCTTT
TAACCATGAGCGATACCGAAACCATGGCCCGGACGATTATGTCGACCGAGCAGTGGGAAAAATTTGCGAAAATGGGTGAA
CTGGACTTTGCCTACTCTCTACCGGATGTGGGCCGTTTTCGGGTAAACGTCTTTCGCCAGCGAGGAGCGGTAGCTTTAGT
TCTCCGGCATATTAATTCGAAAATATTGTCCTTTAAAGAGTTGGGCCTTCCGGAAGTTATTGCCGATCTGTCCCGGAAAA
ATCGGGGGATTGTCCTGGTAACCGGTCCCACCGGTAGCGGTAAATCAACCACCCTGGCTTCAATGATTGATTTAATCAAT
AGCGAGCGAGCCTGTCACATCATAACCCTGGAAGATCCGATTGAATATCTGCATACCCATAAAAAAAGTGTGGTAAACCA
GCGGGAAATAGGCAGTGATACCGAAAGCTTTGCCAAGGCTTTGCGGGCTGCGATGCGGGAAGATCCGGACGTCATCCTTG
TGGGTGAGATGCGGGACTTAGAAACTATTTCTATCGCGATTACGGCGGCAGAAACTGGACACCTGGTTTTTGCGACCCTG
CACACTTCCAGTGCTGCGGAAACCATTGACCGGATCATAGACGTTTTCCCCCCTTCCCAGCAGCAGCAAATTCGCGTACA
GCTTTCCACCACCATTCAGGGGATAATTGCCCAGCAGTTAATTCCCCGGATGGATGGTAAAGGCCGGGTAGTGGCGGTGG
AGGTCATGGTGGCAACTCCAGCAATAAGAAACCTTATCCGGGAGGGTAAAACCCACCAAATCCCTGCCCAGATTCAAACC
GGGGCTAAATACGGAATGCAGACGTTAGATATGGCATTAGCGAAACTTTATCAAAATCGGCTTATTTCTTATGAAGAAGC
TATTTCCCGGGCCCATGATCCCGAAAGTTTAGCCCGAATGTTATATTAA

Upstream 100 bases:

>100_bases
GGAAGACGGGCGGGAAAAAGTTCTGGCAGGTATTACCGCGCCGGAAGAGATTATCCGGGTGGCCAGTACGGTTAACTAAA
AGGTGGCAGGTGAAGTTTTT

Downstream 100 bases:

>100_bases
ATTTGGGAAAAATTTTTGTCGAAGAAAATTACAAAATATATTAAAATATTAAACAAAAGGAATAGTTTGTGAAGGTTATA
AGGAAAGGAGGAGAGTTATG

Product: type IV pilus retraction protein PilT

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 362; Mature: 362

Protein sequence:

>362_residues
MINELLRLMVEFKASDLHLTVNFPPAYRIHGNIRPLTELARENPGIPQSLTRLLTMSDTETMARTIMSTEQWEKFAKMGE
LDFAYSLPDVGRFRVNVFRQRGAVALVLRHINSKILSFKELGLPEVIADLSRKNRGIVLVTGPTGSGKSTTLASMIDLIN
SERACHIITLEDPIEYLHTHKKSVVNQREIGSDTESFAKALRAAMREDPDVILVGEMRDLETISIAITAAETGHLVFATL
HTSSAAETIDRIIDVFPPSQQQQIRVQLSTTIQGIIAQQLIPRMDGKGRVVAVEVMVATPAIRNLIREGKTHQIPAQIQT
GAKYGMQTLDMALAKLYQNRLISYEEAISRAHDPESLARMLY

Sequences:

>Translated_362_residues
MINELLRLMVEFKASDLHLTVNFPPAYRIHGNIRPLTELARENPGIPQSLTRLLTMSDTETMARTIMSTEQWEKFAKMGE
LDFAYSLPDVGRFRVNVFRQRGAVALVLRHINSKILSFKELGLPEVIADLSRKNRGIVLVTGPTGSGKSTTLASMIDLIN
SERACHIITLEDPIEYLHTHKKSVVNQREIGSDTESFAKALRAAMREDPDVILVGEMRDLETISIAITAAETGHLVFATL
HTSSAAETIDRIIDVFPPSQQQQIRVQLSTTIQGIIAQQLIPRMDGKGRVVAVEVMVATPAIRNLIREGKTHQIPAQIQT
GAKYGMQTLDMALAKLYQNRLISYEEAISRAHDPESLARMLY
>Mature_362_residues
MINELLRLMVEFKASDLHLTVNFPPAYRIHGNIRPLTELARENPGIPQSLTRLLTMSDTETMARTIMSTEQWEKFAKMGE
LDFAYSLPDVGRFRVNVFRQRGAVALVLRHINSKILSFKELGLPEVIADLSRKNRGIVLVTGPTGSGKSTTLASMIDLIN
SERACHIITLEDPIEYLHTHKKSVVNQREIGSDTESFAKALRAAMREDPDVILVGEMRDLETISIAITAAETGHLVFATL
HTSSAAETIDRIIDVFPPSQQQQIRVQLSTTIQGIIAQQLIPRMDGKGRVVAVEVMVATPAIRNLIREGKTHQIPAQIQT
GAKYGMQTLDMALAKLYQNRLISYEEAISRAHDPESLARMLY

Specific function: May be involved in pilus retraction [H]

COG id: COG2805

COG function: function code NU; Tfp pilus assembly protein, pilus retraction ATPase PilT

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI87082188, Length=341, Percent_Identity=42.5219941348974, Blast_Score=277, Evalue=8e-76,
Organism=Escherichia coli, GI1789723, Length=305, Percent_Identity=32.7868852459016, Blast_Score=133, Evalue=2e-32,
Organism=Escherichia coli, GI1786296, Length=230, Percent_Identity=38.2608695652174, Blast_Score=122, Evalue=2e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR006321
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 40391; Mature: 40391

Theoretical pI: Translated: 7.88; Mature: 7.88

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINELLRLMVEFKASDLHLTVNFPPAYRIHGNIRPLTELARENPGIPQSLTRLLTMSDTE
CHHHHHHHHHHHCCCCEEEEEECCCCEEECCCCHHHHHHHHCCCCCHHHHHHHHHCCCHH
TMARTIMSTEQWEKFAKMGELDFAYSLPDVGRFRVNVFRQRGAVALVLRHINSKILSFKE
HHHHHHHHHHHHHHHHHCCCCCEEECCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
LGLPEVIADLSRKNRGIVLVTGPTGSGKSTTLASMIDLINSERACHIITLEDPIEYLHTH
CCCHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHH
KKSVVNQREIGSDTESFAKALRAAMREDPDVILVGEMRDLETISIAITAAETGHLVFATL
HHHHHHHHHCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCEEEEEEEEEECCCCEEEEEE
HTSSAAETIDRIIDVFPPSQQQQIRVQLSTTIQGIIAQQLIPRMDGKGRVVAVEVMVATP
ECCHHHHHHHHHHHCCCCCCCHHEEEHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEHHCH
AIRNLIREGKTHQIPAQIQTGAKYGMQTLDMALAKLYQNRLISYEEAISRAHDPESLARM
HHHHHHHCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCHHHHHHH
LY
CC
>Mature Secondary Structure
MINELLRLMVEFKASDLHLTVNFPPAYRIHGNIRPLTELARENPGIPQSLTRLLTMSDTE
CHHHHHHHHHHHCCCCEEEEEECCCCEEECCCCHHHHHHHHCCCCCHHHHHHHHHCCCHH
TMARTIMSTEQWEKFAKMGELDFAYSLPDVGRFRVNVFRQRGAVALVLRHINSKILSFKE
HHHHHHHHHHHHHHHHHCCCCCEEECCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
LGLPEVIADLSRKNRGIVLVTGPTGSGKSTTLASMIDLINSERACHIITLEDPIEYLHTH
CCCHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHH
KKSVVNQREIGSDTESFAKALRAAMREDPDVILVGEMRDLETISIAITAAETGHLVFATL
HHHHHHHHHCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCEEEEEEEEEECCCCEEEEEE
HTSSAAETIDRIIDVFPPSQQQQIRVQLSTTIQGIIAQQLIPRMDGKGRVVAVEVMVATP
ECCHHHHHHHHHHHCCCCCCCHHEEEHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEHHCH
AIRNLIREGKTHQIPAQIQTGAKYGMQTLDMALAKLYQNRLISYEEAISRAHDPESLARM
HHHHHHHCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCHHHHHHH
LY
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1676385; 10984043 [H]