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The map label for this gene is gap [H]

Identifier: 78043763

GI number: 78043763

Start: 246170

End: 247177

Strand: Direct

Name: gap [H]

Synonym: CHY_0280

Alternate gene names: 78043763

Gene position: 246170-247177 (Clockwise)

Preceding gene: 78044494

Following gene: 78044457

Centisome position: 10.25

GC content: 44.94

Gene sequence:

>1008_bases
ATGCCTGTAAAAGTAGGGATTAATGGTTTTGGCCGGATTGGCCGGAATATGTTTCGGGCAGCCTTAAACAATCCGGAAAT
TGAAATTGTAGCGGTAAATGACTTAACCGATGCCAAGACTTTGGCCCACCTCTTGAAATACGACTCGGTTCACGGGATTT
TTGCTGCTGATGTGGAGGCCAAAGATGATGCTATCGTGGTAAATGGTAAAGAAGTTAAAGTTTATGCGGAAAAAGACCCG
GCTAACTTACCCTGGAAGGAGTTAGGGGTAGAAATTGTTATTGAATCTACCGGAAAATTTACCAAAAAGGCTGATGCGGT
AAAACACCTGGAAGCTGGAGCCAAAAAGGTAATCATTTCTGCGCCTGCTACCGATGAAGATATTACTATCGTTATGGGAG
TAAATGAGGATAAGTACGATCCGGCGAACCACCATGTAATTTCCAATGCTTCCTGTACAACTAACTGCCTGGCGCCGGTG
GCCAAAGTGCTGCATGAAAAGTTTGGGATTGTTAAAGGCTTGATGACTACCGTCCACTCTTACACTAACGACCAGCGGAT
TTTAGACCTTCCTCACAAAGATTTACGTCGGGCCCGGGCTGCAGCTACTTCCATAATCCCCACCACAACCGGAGCGGCAA
AAGCGGTGGCCTTAGTTCTTCCCGAATTAAAAGGAAAATTAAACGGCTTTGCCATGCGGGTACCTACACCCAATGTTTCG
GTAGTTGACTTAGTGGTGGAGTTAGAAAAACCCGCAACCAAGGAAGAAATTAACGCTGCTTTAAAGGAAGCTGCCGAAGG
AGAATTAAAGGGCATCCTTGGTTATACCGAGCTCCCCTTGGTTTCGGTGGACTTTAACGGTAATCCTTTGAGCTCTATTG
TTGATGGACTTTCTACCATGGTAATTGAAGACAATATGGCCAAAGTTATTGCCTGGTACGATAATGAATGGGGTTATTCC
AACCGTCTGGTGGACTTAGCGACTTACATTGCCAAAAAAGGTTTATAA

Upstream 100 bases:

>100_bases
ATTACTTTAAAACTTGCCTACCTTAGAAATATCGTTTTATAATACAAGAGAAGCATAGTTATTTTTTTCTAATTTAATTT
AATATAAAGGAGGAATTGGG

Downstream 100 bases:

>100_bases
TAGTGGGGGCCAAGTCCCCCGTTTCCCATTTTAAGAAGGGAAGGGGAGTGTTATGAACAAAAAGTCTATAAGAGATGTGG
AGGTAAAAGGCAAAAAAGTT

Product: glyceraldehyde-3-phosphate dehydrogenase

Products: NA

Alternate protein names: GAPDH [H]

Number of amino acids: Translated: 335; Mature: 334

Protein sequence:

>335_residues
MPVKVGINGFGRIGRNMFRAALNNPEIEIVAVNDLTDAKTLAHLLKYDSVHGIFAADVEAKDDAIVVNGKEVKVYAEKDP
ANLPWKELGVEIVIESTGKFTKKADAVKHLEAGAKKVIISAPATDEDITIVMGVNEDKYDPANHHVISNASCTTNCLAPV
AKVLHEKFGIVKGLMTTVHSYTNDQRILDLPHKDLRRARAAATSIIPTTTGAAKAVALVLPELKGKLNGFAMRVPTPNVS
VVDLVVELEKPATKEEINAALKEAAEGELKGILGYTELPLVSVDFNGNPLSSIVDGLSTMVIEDNMAKVIAWYDNEWGYS
NRLVDLATYIAKKGL

Sequences:

>Translated_335_residues
MPVKVGINGFGRIGRNMFRAALNNPEIEIVAVNDLTDAKTLAHLLKYDSVHGIFAADVEAKDDAIVVNGKEVKVYAEKDP
ANLPWKELGVEIVIESTGKFTKKADAVKHLEAGAKKVIISAPATDEDITIVMGVNEDKYDPANHHVISNASCTTNCLAPV
AKVLHEKFGIVKGLMTTVHSYTNDQRILDLPHKDLRRARAAATSIIPTTTGAAKAVALVLPELKGKLNGFAMRVPTPNVS
VVDLVVELEKPATKEEINAALKEAAEGELKGILGYTELPLVSVDFNGNPLSSIVDGLSTMVIEDNMAKVIAWYDNEWGYS
NRLVDLATYIAKKGL
>Mature_334_residues
PVKVGINGFGRIGRNMFRAALNNPEIEIVAVNDLTDAKTLAHLLKYDSVHGIFAADVEAKDDAIVVNGKEVKVYAEKDPA
NLPWKELGVEIVIESTGKFTKKADAVKHLEAGAKKVIISAPATDEDITIVMGVNEDKYDPANHHVISNASCTTNCLAPVA
KVLHEKFGIVKGLMTTVHSYTNDQRILDLPHKDLRRARAAATSIIPTTTGAAKAVALVLPELKGKLNGFAMRVPTPNVSV
VDLVVELEKPATKEEINAALKEAAEGELKGILGYTELPLVSVDFNGNPLSSIVDGLSTMVIEDNMAKVIAWYDNEWGYSN
RLVDLATYIAKKGL

Specific function: Second phase of glycolysis; first step. [C]

COG id: COG0057

COG function: function code G; Glyceraldehyde-3-phosphate dehydrogenase/erythrose-4-phosphate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glyceraldehyde-3-phosphate dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI7669492, Length=333, Percent_Identity=53.4534534534535, Blast_Score=355, Evalue=2e-98,
Organism=Homo sapiens, GI7657116, Length=331, Percent_Identity=51.6616314199396, Blast_Score=345, Evalue=3e-95,
Organism=Escherichia coli, GI1788079, Length=333, Percent_Identity=57.957957957958, Blast_Score=395, Evalue=1e-111,
Organism=Escherichia coli, GI1789295, Length=329, Percent_Identity=47.4164133738602, Blast_Score=323, Evalue=8e-90,
Organism=Caenorhabditis elegans, GI17534679, Length=337, Percent_Identity=53.7091988130564, Blast_Score=350, Evalue=4e-97,
Organism=Caenorhabditis elegans, GI17534677, Length=337, Percent_Identity=53.4124629080119, Blast_Score=350, Evalue=5e-97,
Organism=Caenorhabditis elegans, GI32566163, Length=336, Percent_Identity=52.6785714285714, Blast_Score=338, Evalue=2e-93,
Organism=Caenorhabditis elegans, GI17568413, Length=336, Percent_Identity=52.6785714285714, Blast_Score=338, Evalue=2e-93,
Organism=Saccharomyces cerevisiae, GI6321631, Length=332, Percent_Identity=53.9156626506024, Blast_Score=351, Evalue=7e-98,
Organism=Saccharomyces cerevisiae, GI6322468, Length=332, Percent_Identity=53.0120481927711, Blast_Score=347, Evalue=2e-96,
Organism=Saccharomyces cerevisiae, GI6322409, Length=332, Percent_Identity=52.4096385542169, Blast_Score=346, Evalue=3e-96,
Organism=Drosophila melanogaster, GI85725000, Length=332, Percent_Identity=53.6144578313253, Blast_Score=340, Evalue=9e-94,
Organism=Drosophila melanogaster, GI22023983, Length=332, Percent_Identity=53.6144578313253, Blast_Score=340, Evalue=9e-94,
Organism=Drosophila melanogaster, GI17933600, Length=332, Percent_Identity=53.3132530120482, Blast_Score=338, Evalue=2e-93,
Organism=Drosophila melanogaster, GI18110149, Length=332, Percent_Identity=53.3132530120482, Blast_Score=338, Evalue=2e-93,
Organism=Drosophila melanogaster, GI19922412, Length=327, Percent_Identity=51.0703363914373, Blast_Score=335, Evalue=3e-92,

Paralogues:

None

Copy number: 220 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1840 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 740 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Min

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020831
- InterPro:   IPR020830
- InterPro:   IPR020829
- InterPro:   IPR020828
- InterPro:   IPR006424
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02800 Gp_dh_C; PF00044 Gp_dh_N [H]

EC number: =1.2.1.12 [H]

Molecular weight: Translated: 36159; Mature: 36028

Theoretical pI: Translated: 6.12; Mature: 6.12

Prosite motif: PS00071 GAPDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVKVGINGFGRIGRNMFRAALNNPEIEIVAVNDLTDAKTLAHLLKYDSVHGIFAADVEA
CCEEECCCCHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHCCCCCEEEECCCC
KDDAIVVNGKEVKVYAEKDPANLPWKELGVEIVIESTGKFTKKADAVKHLEAGAKKVIIS
CCCEEEEECCEEEEEECCCCCCCCHHHCCEEEEEECCCCCHHHHHHHHHHHCCCCEEEEE
APATDEDITIVMGVNEDKYDPANHHVISNASCTTNCLAPVAKVLHEKFGIVKGLMTTVHS
CCCCCCCEEEEEECCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
YTNDQRILDLPHKDLRRARAAATSIIPTTTGAAKAVALVLPELKGKLNGFAMRVPTPNVS
CCCCCEEEECCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCEEEECCCCCCE
VVDLVVELEKPATKEEINAALKEAAEGELKGILGYTELPLVSVDFNGNPLSSIVDGLSTM
EEEEEEECCCCCCHHHHHHHHHHHHCCCHHEEECCCCCCEEEEECCCCCHHHHHHHHHHE
VIEDNMAKVIAWYDNEWGYSNRLVDLATYIAKKGL
EEECCCEEEEEEECCCCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
PVKVGINGFGRIGRNMFRAALNNPEIEIVAVNDLTDAKTLAHLLKYDSVHGIFAADVEA
CEEECCCCHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHCCCCCEEEECCCC
KDDAIVVNGKEVKVYAEKDPANLPWKELGVEIVIESTGKFTKKADAVKHLEAGAKKVIIS
CCCEEEEECCEEEEEECCCCCCCCHHHCCEEEEEECCCCCHHHHHHHHHHHCCCCEEEEE
APATDEDITIVMGVNEDKYDPANHHVISNASCTTNCLAPVAKVLHEKFGIVKGLMTTVHS
CCCCCCCEEEEEECCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
YTNDQRILDLPHKDLRRARAAATSIIPTTTGAAKAVALVLPELKGKLNGFAMRVPTPNVS
CCCCCEEEECCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCEEEECCCCCCE
VVDLVVELEKPATKEEINAALKEAAEGELKGILGYTELPLVSVDFNGNPLSSIVDGLSTM
EEEEEEECCCCCCHHHHHHHHHHHHCCCHHEEECCCCCCEEEEECCCCCHHHHHHHHHHE
VIEDNMAKVIAWYDNEWGYSNRLVDLATYIAKKGL
EEECCCEEEEEEECCCCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2684782; 2227448; 2656407; 7408868; 193030; 3586018; 9175858 [H]