Click here to switch to the map view.

The map label for this gene is gcvH

Identifier: 78044435

GI number: 78044435

Start: 425067

End: 425459

Strand: Direct

Name: gcvH

Synonym: CHY_0490

Alternate gene names: 78044435

Gene position: 425067-425459 (Clockwise)

Preceding gene: 78042904

Following gene: 78044154

Centisome position: 17.7

GC content: 43.26

Gene sequence:

>393_bases
ATGAGTGTGGTACCTAAGGATTTAAAGTACAGCCGTGAGCATGAGTGGATTAAGGTAGATGGCAATATCGGTATTATCGG
TATTACCGATTTTGCCCAAAAATCTTTGGGAGATATCGTATTTATTGAGCTTCCCGGTGTGGGGGATGAAATTTCCGCCG
GAGATAGCTTTGGAGTTGTAGAGTCGGTAAAAGCTGCTTCCGACTTATATGCTCCCGTTTCCGGAAAAGTTGTGGAAGTT
AATGAAGAAGTTATTGAATCGCCGCAACTGGTAAATGAAGATCCCTATGGTAAGGGGTGGATGATTAAAGTGGAAATTAG
TAACGAAGCGGAGTTAGACGAACTGCTCTCCCCCGAAGATTACGAAAAACTTCTGGAAGAGGAAGGAGAGTAA

Upstream 100 bases:

>100_bases
AGGCGGCGGAAATTGGTACCGAGGTGGACGTAATCATTCGGGGGAAGGGCGTGCGTGCCCGCGTAATTTCAAGACCATTT
TATAAAAGGGGGAAATAAGC

Downstream 100 bases:

>100_bases
TTTGAAATATACTCCCCATACCCCGGACGAAGTCCGGGAAATGTTAAGTAGCCTTGGCTTAAGTAGCATCGAGGAATTGT
TTTCGGACATCCCGGAAGAG

Product: glycine cleavage system H protein

Products: Proton; NADH; NH3; CO2; 5,10-methylene-THF [C]

Alternate protein names: NA

Number of amino acids: Translated: 130; Mature: 129

Protein sequence:

>130_residues
MSVVPKDLKYSREHEWIKVDGNIGIIGITDFAQKSLGDIVFIELPGVGDEISAGDSFGVVESVKAASDLYAPVSGKVVEV
NEEVIESPQLVNEDPYGKGWMIKVEISNEAELDELLSPEDYEKLLEEEGE

Sequences:

>Translated_130_residues
MSVVPKDLKYSREHEWIKVDGNIGIIGITDFAQKSLGDIVFIELPGVGDEISAGDSFGVVESVKAASDLYAPVSGKVVEV
NEEVIESPQLVNEDPYGKGWMIKVEISNEAELDELLSPEDYEKLLEEEGE
>Mature_129_residues
SVVPKDLKYSREHEWIKVDGNIGIIGITDFAQKSLGDIVFIELPGVGDEISAGDSFGVVESVKAASDLYAPVSGKVVEVN
EEVIESPQLVNEDPYGKGWMIKVEISNEAELDELLSPEDYEKLLEEEGE

Specific function: The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein

COG id: COG0509

COG function: function code E; Glycine cleavage system H protein (lipoate-binding)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain

Homologues:

Organism=Homo sapiens, GI49574537, Length=118, Percent_Identity=50.8474576271186, Blast_Score=136, Evalue=5e-33,
Organism=Homo sapiens, GI89057342, Length=118, Percent_Identity=50, Blast_Score=133, Evalue=4e-32,
Organism=Escherichia coli, GI1789271, Length=130, Percent_Identity=55.3846153846154, Blast_Score=145, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI17551294, Length=119, Percent_Identity=53.781512605042, Blast_Score=138, Evalue=9e-34,
Organism=Caenorhabditis elegans, GI17507493, Length=119, Percent_Identity=51.2605042016807, Blast_Score=132, Evalue=6e-32,
Organism=Saccharomyces cerevisiae, GI6319272, Length=125, Percent_Identity=45.6, Blast_Score=117, Evalue=7e-28,
Organism=Drosophila melanogaster, GI17865652, Length=130, Percent_Identity=45.3846153846154, Blast_Score=127, Evalue=2e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GCSH_CARHZ (Q3AET6)

Other databases:

- EMBL:   CP000141
- RefSeq:   YP_359348.1
- ProteinModelPortal:   Q3AET6
- SMR:   Q3AET6
- STRING:   Q3AET6
- GeneID:   3727553
- GenomeReviews:   CP000141_GR
- KEGG:   chy:CHY_0490
- NMPDR:   fig|246194.3.peg.975
- TIGR:   CHY_0490
- eggNOG:   COG0509
- HOGENOM:   HBG693789
- OMA:   TPKELRY
- ProtClustDB:   CLSK2772327
- BioCyc:   CHYD246194:CHY_0490-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_00272
- InterPro:   IPR003016
- InterPro:   IPR002930
- InterPro:   IPR017453
- InterPro:   IPR011053
- PANTHER:   PTHR11715
- TIGRFAMs:   TIGR00527

Pfam domain/function: PF01597 GCV_H; SSF51230 Hybrid_motif

EC number: NA

Molecular weight: Translated: 14256; Mature: 14125

Theoretical pI: Translated: 3.82; Mature: 3.82

Prosite motif: PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVVPKDLKYSREHEWIKVDGNIGIIGITDFAQKSLGDIVFIELPGVGDEISAGDSFGVV
CCCCCCCCCCCCCCCEEEECCCEEEEEECHHHHHCCCCEEEEEECCCCCCCCCCCCCCHH
ESVKAASDLYAPVSGKVVEVNEEVIESPQLVNEDPYGKGWMIKVEISNEAELDELLSPED
HHHHHHHHCCCCCCCEEEEECHHHHCCCCCCCCCCCCCCEEEEEEECCCCCHHHHCCCHH
YEKLLEEEGE
HHHHHHHCCC
>Mature Secondary Structure 
SVVPKDLKYSREHEWIKVDGNIGIIGITDFAQKSLGDIVFIELPGVGDEISAGDSFGVV
CCCCCCCCCCCCCCEEEECCCEEEEEECHHHHHCCCCEEEEEECCCCCCCCCCCCCCHH
ESVKAASDLYAPVSGKVVEVNEEVIESPQLVNEDPYGKGWMIKVEISNEAELDELLSPED
HHHHHHHHCCCCCCCEEEEECHHHHCCCCCCCCCCCCCCEEEEEEECCCCCHHHHCCCHH
YEKLLEEEGE
HHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Lipoyl Cofactor. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD; L-glycine; THF [C]

Specific reaction: NAD + L-glycine + THF = Proton + NADH + NH3 + CO2 + 5,10-methylene-THF [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA