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The map label for this gene is fdoH [H]

Identifier: 78044396

GI number: 78044396

Start: 705121

End: 705903

Strand: Direct

Name: fdoH [H]

Synonym: CHY_0794

Alternate gene names: 78044396

Gene position: 705121-705903 (Clockwise)

Preceding gene: 78042637

Following gene: 78043392

Centisome position: 29.36

GC content: 46.62

Gene sequence:

>783_bases
ATGAACGGCATGTTTATTGATACATCCCTTTGTGTTGGCTGCCGGGGCTGCATGACTGCCTGCAAAAGCTGGAACGAATT
ACCCGGAGAAAAAACGACTTTTACCGGAAGTTTACAGAGTATGCCCGATACTACCCCTAAAACTTATACATTGATTAAAT
TTTATGAACGTGAAGAAAACGGGCGGATTCGTTTTGACTTTTTTAAACAGCAGTGTTTTCACTGCGGTGATGCGGCTTGT
GAAAAAGCTTGTCCGGAAGATGCAATCTATCATACCAAAGAAGGGGCGGTAGTGCGGGATTACGACCGGTGTATAGGCTG
TGATTACTGCCAGAGGGCGTGCCCCTTTAATATCCCGCGGATTGACACTCAAAACAAGAAAATGCATAAATGCACCTTGT
GTTTTGACCGGTTAGAAAACGGAGAAATTCCGGCTTGTGCCCTGACTTGTTCTCCGGGAGCTATTAAATACGGCAACCGG
GATGAGCTGTGGGAGGCAGCCCAAAAGCGGCTGGCGGAGGTTAAAAAAGAGTATCCCAAGGCTAATCTTTATCCTTCCGA
TAAAAATGCTTTGGGAGGAACCGGGGTATTTTATCTTTTAATTAACGAGCCGGAGTTCTACGGTCTTCCGGCCGAGGCAA
AATTAAAAACTACCTTTTCCCTCTGGCATGACGTGGTGAGACCTTTAGGGAAAATTGTGCCAGGGATAGCGCTTGGGGCT
ACTGTTATAGCGGCGGTAGCTAATTCGGTAAAAAAACAACGGGAGGAGGGACACGGTGAGTAA

Upstream 100 bases:

>100_bases
CCGGAGAAATTGCCAATAAACTTACTCCTTATCTGGGGGATGCCAATACCATGATTCCCGAATACAAGGGGTTCCTGGTC
AACGTAAGGAGGGCTAAATA

Downstream 100 bases:

>100_bases
TAAAGTTTTCCGGTTTAATAAAACAATGCGTTTTTTTCACTGGTCGTATATTACCTTTTATTTTCTTTTAGCTATTACCG
GAGCTTTTTTATACTTTGAT

Product: formate dehydrogenase-O, iron-sulfur subunit

Products: CO2; NADH

Alternate protein names: Anaerobic formate dehydrogenase iron-sulfur subunit; Formate dehydrogenase-N subunit beta; FDH-N subunit beta [H]

Number of amino acids: Translated: 260; Mature: 260

Protein sequence:

>260_residues
MNGMFIDTSLCVGCRGCMTACKSWNELPGEKTTFTGSLQSMPDTTPKTYTLIKFYEREENGRIRFDFFKQQCFHCGDAAC
EKACPEDAIYHTKEGAVVRDYDRCIGCDYCQRACPFNIPRIDTQNKKMHKCTLCFDRLENGEIPACALTCSPGAIKYGNR
DELWEAAQKRLAEVKKEYPKANLYPSDKNALGGTGVFYLLINEPEFYGLPAEAKLKTTFSLWHDVVRPLGKIVPGIALGA
TVIAAVANSVKKQREEGHGE

Sequences:

>Translated_260_residues
MNGMFIDTSLCVGCRGCMTACKSWNELPGEKTTFTGSLQSMPDTTPKTYTLIKFYEREENGRIRFDFFKQQCFHCGDAAC
EKACPEDAIYHTKEGAVVRDYDRCIGCDYCQRACPFNIPRIDTQNKKMHKCTLCFDRLENGEIPACALTCSPGAIKYGNR
DELWEAAQKRLAEVKKEYPKANLYPSDKNALGGTGVFYLLINEPEFYGLPAEAKLKTTFSLWHDVVRPLGKIVPGIALGA
TVIAAVANSVKKQREEGHGE
>Mature_260_residues
MNGMFIDTSLCVGCRGCMTACKSWNELPGEKTTFTGSLQSMPDTTPKTYTLIKFYEREENGRIRFDFFKQQCFHCGDAAC
EKACPEDAIYHTKEGAVVRDYDRCIGCDYCQRACPFNIPRIDTQNKKMHKCTLCFDRLENGEIPACALTCSPGAIKYGNR
DELWEAAQKRLAEVKKEYPKANLYPSDKNALGGTGVFYLLINEPEFYGLPAEAKLKTTFSLWHDVVRPLGKIVPGIALGA
TVIAAVANSVKKQREEGHGE

Specific function: Formate dehydrogenase allows E.coli to use formate as major electron donor during anaerobic respiration, when nitrate is used as electron acceptor. The beta chain is an electron transfer unit containing 4 cysteine clusters involved in the formation of iro

COG id: COG0437

COG function: function code C; Fe-S-cluster-containing hydrogenase components 1

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 4 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1787749, Length=264, Percent_Identity=38.6363636363636, Blast_Score=189, Evalue=2e-49,
Organism=Escherichia coli, GI1790326, Length=257, Percent_Identity=35.408560311284, Blast_Score=176, Evalue=1e-45,
Organism=Escherichia coli, GI1789370, Length=292, Percent_Identity=30.8219178082192, Blast_Score=125, Evalue=2e-30,
Organism=Escherichia coli, GI1787872, Length=206, Percent_Identity=32.0388349514563, Blast_Score=103, Evalue=9e-24,
Organism=Escherichia coli, GI1787122, Length=206, Percent_Identity=33.0097087378641, Blast_Score=103, Evalue=9e-24,
Organism=Escherichia coli, GI2367345, Length=162, Percent_Identity=32.7160493827161, Blast_Score=94, Evalue=6e-21,
Organism=Escherichia coli, GI226510944, Length=160, Percent_Identity=31.25, Blast_Score=88, Evalue=5e-19,
Organism=Escherichia coli, GI1787963, Length=148, Percent_Identity=29.7297297297297, Blast_Score=64, Evalue=7e-12,
Organism=Escherichia coli, GI1787740, Length=92, Percent_Identity=34.7826086956522, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1787478, Length=92, Percent_Identity=35.8695652173913, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1788811, Length=183, Percent_Identity=28.9617486338798, Blast_Score=61, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR006470
- InterPro:   IPR014603
- InterPro:   IPR015246 [H]

Pfam domain/function: PF09163 Form-deh_trans [H]

EC number: 1.2.1.2

Molecular weight: Translated: 29055; Mature: 29055

Theoretical pI: Translated: 7.48; Mature: 7.48

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

6.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
8.1 %Cys+Met (Translated Protein)
6.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
8.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNGMFIDTSLCVGCRGCMTACKSWNELPGEKTTFTGSLQSMPDTTPKTYTLIKFYEREEN
CCCEEECHHHHHHHHHHHHHHHHHHHCCCCCCEECCCHHCCCCCCCCCEEEEEEEEECCC
GRIRFDFFKQQCFHCGDAACEKACPEDAIYHTKEGAVVRDYDRCIGCDYCQRACPFNIPR
CCEEHHHHHHHHHHCCHHHHHHCCCCHHHEECCCCCEEECHHHHCCCHHHHHCCCCCCCC
IDTQNKKMHKCTLCFDRLENGEIPACALTCSPGAIKYGNRDELWEAAQKRLAEVKKEYPK
CCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCC
ANLYPSDKNALGGTGVFYLLINEPEFYGLPAEAKLKTTFSLWHDVVRPLGKIVPGIALGA
CCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHH
TVIAAVANSVKKQREEGHGE
HHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNGMFIDTSLCVGCRGCMTACKSWNELPGEKTTFTGSLQSMPDTTPKTYTLIKFYEREEN
CCCEEECHHHHHHHHHHHHHHHHHHHCCCCCCEECCCHHCCCCCCCCCEEEEEEEEECCC
GRIRFDFFKQQCFHCGDAACEKACPEDAIYHTKEGAVVRDYDRCIGCDYCQRACPFNIPR
CCEEHHHHHHHHHHCCHHHHHHCCCCHHHEECCCCCEEECHHHHCCCHHHHHCCCCCCCC
IDTQNKKMHKCTLCFDRLENGEIPACALTCSPGAIKYGNRDELWEAAQKRLAEVKKEYPK
CCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCC
ANLYPSDKNALGGTGVFYLLINEPEFYGLPAEAKLKTTFSLWHDVVRPLGKIVPGIALGA
CCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHH
TVIAAVANSVKKQREEGHGE
HHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; NAD+

Specific reaction: formate + NAD+ = CO2 + NADH

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1834669; 9097039; 9278503; 11884747 [H]