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The map label for this gene is fdoI [H]

Identifier: 78043392

GI number: 78043392

Start: 705896

End: 706591

Strand: Direct

Name: fdoI [H]

Synonym: CHY_0795

Alternate gene names: 78043392

Gene position: 705896-706591 (Clockwise)

Preceding gene: 78044396

Following gene: 78043086

Centisome position: 29.39

GC content: 39.51

Gene sequence:

>696_bases
GTGAGTAATAAAGTTTTCCGGTTTAATAAAACAATGCGTTTTTTTCACTGGTCGTATATTACCTTTTATTTTCTTTTAGC
TATTACCGGAGCTTTTTTATACTTTGATTTTCTTGATTGGTTAAGACCCCTTTTTGGCGGAGCTCAGGGGGCCAGAATTG
TTCACCGGGTAGCAGCGGTGGGGCTGATCCTTTCGCCGATTATTTCTTTCCTTTTAAATCCCAAAGAAATTTGGGAAACT
TTAAAATACGCCTACTCCTGGAAAGCATCGGATCTAAAGTTTTTTGGCGAGTTTATCAAAGAGTTCTTTGGGGGTCATGC
CCATTATGAACCCCAGGGTAAATACAATCAGGGAGAAAAATTAAACATTGTTTTACAAAGTTTGGGATGGCTTTTATTTA
TCGTCAGTGGTATAGTACTATGGCGTTTTGAAAGCTTTTCACCCGAAGTGGGTATGATCGCCTTAATCGGCCATGATATA
GCGTTTATCTTTACCCTGGCTTATGTTATTGGCCATGTCTACCTGTCCCTCTTCCATCCGGTTACCAGACCGGCTCTTAA
AGGGATGCTTACTGGTTACGTGGATGAAGAATATGCTAAAAATCACCATCCTTTGTGGTATAATGAGGTAAAACAGGAAA
AACAGGTTAGGGGAAAAGAAAAAACTAAAACCAAAGCGCTTTCTCTTGAAAAATGA

Upstream 100 bases:

>100_bases
GTGAGACCTTTAGGGAAAATTGTGCCAGGGATAGCGCTTGGGGCTACTGTTATAGCGGCGGTAGCTAATTCGGTAAAAAA
ACAACGGGAGGAGGGACACG

Downstream 100 bases:

>100_bases
TAAGCGGGGGCATTTACTGCCCCCGGTTTTATCTTTTTAGAAAATGGGGGAGAAAATATGGATGCTAAAAAACTTGTAAG
CACTATGGAAAAGTTAATTG

Product: formate dehydrogenase, cytochrome b556 (fdo) subunit

Products: CO2; NADH2 [C]

Alternate protein names: Aerobic formate dehydrogenase cytochrome b556 subunit; FDH-Z subunit gamma; Formate dehydrogenase-O subunit gamma [H]

Number of amino acids: Translated: 231; Mature: 230

Protein sequence:

>231_residues
MSNKVFRFNKTMRFFHWSYITFYFLLAITGAFLYFDFLDWLRPLFGGAQGARIVHRVAAVGLILSPIISFLLNPKEIWET
LKYAYSWKASDLKFFGEFIKEFFGGHAHYEPQGKYNQGEKLNIVLQSLGWLLFIVSGIVLWRFESFSPEVGMIALIGHDI
AFIFTLAYVIGHVYLSLFHPVTRPALKGMLTGYVDEEYAKNHHPLWYNEVKQEKQVRGKEKTKTKALSLEK

Sequences:

>Translated_231_residues
MSNKVFRFNKTMRFFHWSYITFYFLLAITGAFLYFDFLDWLRPLFGGAQGARIVHRVAAVGLILSPIISFLLNPKEIWET
LKYAYSWKASDLKFFGEFIKEFFGGHAHYEPQGKYNQGEKLNIVLQSLGWLLFIVSGIVLWRFESFSPEVGMIALIGHDI
AFIFTLAYVIGHVYLSLFHPVTRPALKGMLTGYVDEEYAKNHHPLWYNEVKQEKQVRGKEKTKTKALSLEK
>Mature_230_residues
SNKVFRFNKTMRFFHWSYITFYFLLAITGAFLYFDFLDWLRPLFGGAQGARIVHRVAAVGLILSPIISFLLNPKEIWETL
KYAYSWKASDLKFFGEFIKEFFGGHAHYEPQGKYNQGEKLNIVLQSLGWLLFIVSGIVLWRFESFSPEVGMIALIGHDIA
FIFTLAYVIGHVYLSLFHPVTRPALKGMLTGYVDEEYAKNHHPLWYNEVKQEKQVRGKEKTKTKALSLEK

Specific function: Allows to use formate as major electron donor during aerobic respiration. Subunit gamma is probably the cytochrome b556(FDO) component of the formate dehydrogenase [H]

COG id: COG2864

COG function: function code C; Cytochrome b subunit of formate dehydrogenase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the formate dehydrogenase gamma subunit family [H]

Homologues:

Organism=Escherichia coli, GI1790325, Length=220, Percent_Identity=26.3636363636364, Blast_Score=81, Evalue=7e-17,
Organism=Escherichia coli, GI1787750, Length=225, Percent_Identity=25.3333333333333, Blast_Score=78, Evalue=4e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005797
- InterPro:   IPR016174
- InterPro:   IPR006471 [H]

Pfam domain/function: PF00033 Cytochrom_B_N [H]

EC number: NA

Molecular weight: Translated: 26863; Mature: 26731

Theoretical pI: Translated: 9.84; Mature: 9.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNKVFRFNKTMRFFHWSYITFYFLLAITGAFLYFDFLDWLRPLFGGAQGARIVHRVAAV
CCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
GLILSPIISFLLNPKEIWETLKYAYSWKASDLKFFGEFIKEFFGGHAHYEPQGKYNQGEK
HHHHHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCH
LNIVLQSLGWLLFIVSGIVLWRFESFSPEVGMIALIGHDIAFIFTLAYVIGHVYLSLFHP
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VTRPALKGMLTGYVDEEYAKNHHPLWYNEVKQEKQVRGKEKTKTKALSLEK
HHHHHHHHHHHHHCCHHHHCCCCCCCHHHHHHHHHHCCHHHHHHHHHCCCC
>Mature Secondary Structure 
SNKVFRFNKTMRFFHWSYITFYFLLAITGAFLYFDFLDWLRPLFGGAQGARIVHRVAAV
CCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
GLILSPIISFLLNPKEIWETLKYAYSWKASDLKFFGEFIKEFFGGHAHYEPQGKYNQGEK
HHHHHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCH
LNIVLQSLGWLLFIVSGIVLWRFESFSPEVGMIALIGHDIAFIFTLAYVIGHVYLSLFHP
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VTRPALKGMLTGYVDEEYAKNHHPLWYNEVKQEKQVRGKEKTKTKALSLEK
HHHHHHHHHHHHHCCHHHHCCCCCCCHHHHHHHHHHCCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; NAD [C]

Specific reaction: formate + NAD = CO2 + NADH2 [C]

General reaction: Oxidoreductases [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]