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The map label for this gene is cooF2 [H]

Identifier: 78044076

GI number: 78044076

Start: 649247

End: 649732

Strand: Direct

Name: cooF2 [H]

Synonym: CHY_0735

Alternate gene names: 78044076

Gene position: 649247-649732 (Clockwise)

Preceding gene: 78044101

Following gene: 78044052

Centisome position: 27.03

GC content: 43.83

Gene sequence:

>486_bases
TTGCGTGAGATTTTAGTTGATTTAGAAAAATGCCTTGGTTGTAAAACCTGTGAACTGGTTTGTGCTACCTCCCATTCTAA
GACTAAAGAGCTATATACAGCAATCTCAGAAAATCCCCGACCGCAAAGACGGATTTTTGTTGAGGCCAATGGTTCCACCA
ATTTCCCTTTGATGTGCCGGCATTGTGAAGACGCTCCCTGTGTTACCGCTTGCATGAGCGGAGCTTTAACCAAAGATGAA
CTAACCGGTGAAGTGCTGTTAAACTCCCAAAGGTGTATTGGCTGTTTTATGTGCGTGATGGTTTGTCCTTTCGGAGTAAT
TATCGAAAACCAGCATAAAGCCGCGAAATGTGACCGCTGTGCTGAAATTGGCTATCCCCAGTGCGTTTTAGCCTGTCCCA
CCAAAGCCCTGTCCTTTGCAGAAGTTCCCGTTTATGCCAAAAAGGAACGAAAGAAATACTTAACAAATTTTTTGATGAAG
GAGTGA

Upstream 100 bases:

>100_bases
TTTCTTGGAATAAAAATCTAAAAATTTTGAAAAATAATTGCAGGAATTGGCAAAAAGATTTAGAAATATATACTTAGTAC
AAACTTTTGGAGGTGATAAT

Downstream 100 bases:

>100_bases
TTTTATGGATAAAAGCAAATTATCGGTAGACCCGGTAATTCCCAATCTTTATCGGAAAGCCCGGGAAGAAGGGATTTCCA
CCGTTTTTGACCGGTATGAA

Product: iron-sulfur cluster-binding protein CooF

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 161; Mature: 161

Protein sequence:

>161_residues
MREILVDLEKCLGCKTCELVCATSHSKTKELYTAISENPRPQRRIFVEANGSTNFPLMCRHCEDAPCVTACMSGALTKDE
LTGEVLLNSQRCIGCFMCVMVCPFGVIIENQHKAAKCDRCAEIGYPQCVLACPTKALSFAEVPVYAKKERKKYLTNFLMK
E

Sequences:

>Translated_161_residues
MREILVDLEKCLGCKTCELVCATSHSKTKELYTAISENPRPQRRIFVEANGSTNFPLMCRHCEDAPCVTACMSGALTKDE
LTGEVLLNSQRCIGCFMCVMVCPFGVIIENQHKAAKCDRCAEIGYPQCVLACPTKALSFAEVPVYAKKERKKYLTNFLMK
E
>Mature_161_residues
MREILVDLEKCLGCKTCELVCATSHSKTKELYTAISENPRPQRRIFVEANGSTNFPLMCRHCEDAPCVTACMSGALTKDE
LTGEVLLNSQRCIGCFMCVMVCPFGVIIENQHKAAKCDRCAEIGYPQCVLACPTKALSFAEVPVYAKKERKKYLTNFLMK
E

Specific function: The carbon monoxide dehydrogenase (CODH) oxidizes carbon monoxide coupled, via CooF, to the reduction of a hydrogen cation by a hydrogenase (probably CooH). CooF is required in stoichiometric amounts in vitro for anchoring CODH to the membrane as well as

COG id: COG1142

COG function: function code C; Fe-S-cluster-containing hydrogenase components 2

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI87082179, Length=143, Percent_Identity=40.5594405594406, Blast_Score=97, Evalue=7e-22,
Organism=Escherichia coli, GI87082180, Length=140, Percent_Identity=39.2857142857143, Blast_Score=94, Evalue=5e-21,
Organism=Escherichia coli, GI1789067, Length=155, Percent_Identity=38.0645161290323, Blast_Score=93, Evalue=1e-20,
Organism=Escherichia coli, GI2367245, Length=147, Percent_Identity=39.4557823129252, Blast_Score=91, Evalue=4e-20,
Organism=Escherichia coli, GI1787122, Length=157, Percent_Identity=34.3949044585987, Blast_Score=86, Evalue=1e-18,
Organism=Escherichia coli, GI1788811, Length=149, Percent_Identity=34.8993288590604, Blast_Score=85, Evalue=2e-18,
Organism=Escherichia coli, GI1787872, Length=150, Percent_Identity=33.3333333333333, Blast_Score=81, Evalue=4e-17,
Organism=Escherichia coli, GI1789079, Length=193, Percent_Identity=30.5699481865285, Blast_Score=75, Evalue=3e-15,
Organism=Escherichia coli, GI226510944, Length=148, Percent_Identity=35.1351351351351, Blast_Score=74, Evalue=4e-15,
Organism=Escherichia coli, GI1787749, Length=155, Percent_Identity=30.3225806451613, Blast_Score=71, Evalue=4e-14,
Organism=Escherichia coli, GI2367345, Length=95, Percent_Identity=41.0526315789474, Blast_Score=69, Evalue=1e-13,
Organism=Escherichia coli, GI1790326, Length=155, Percent_Identity=29.6774193548387, Blast_Score=64, Evalue=7e-12,
Organism=Escherichia coli, GI87082114, Length=106, Percent_Identity=33.9622641509434, Blast_Score=64, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900 [H]

Pfam domain/function: PF00037 Fer4 [H]

EC number: NA

Molecular weight: Translated: 17971; Mature: 17971

Theoretical pI: Translated: 7.85; Mature: 7.85

Prosite motif: PS00198 4FE4S_FERREDOXIN ; PS00228 TUBULIN_B_AUTOREG

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

9.9 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
13.7 %Cys+Met (Translated Protein)
9.9 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
13.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MREILVDLEKCLGCKTCELVCATSHSKTKELYTAISENPRPQRRIFVEANGSTNFPLMCR
CHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHCCCCCCCEEEEEECCCCCCCCEEEE
HCEDAPCVTACMSGALTKDELTGEVLLNSQRCIGCFMCVMVCPFGVIIENQHKAAKCDRC
CCCCCHHHHHHHHCCCCHHHCCHHHHCCCCHHHHHHHHHHHCCCCEEEECCCCHHHHHHH
AEIGYPQCVLACPTKALSFAEVPVYAKKERKKYLTNFLMKE
HHCCCCEEEEECCHHHHHHHHCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MREILVDLEKCLGCKTCELVCATSHSKTKELYTAISENPRPQRRIFVEANGSTNFPLMCR
CHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHCCCCCCCEEEEEECCCCCCCCEEEE
HCEDAPCVTACMSGALTKDELTGEVLLNSQRCIGCFMCVMVCPFGVIIENQHKAAKCDRC
CCCCCHHHHHHHHCCCCHHHCCHHHHCCCCHHHHHHHHHHHCCCCEEEECCCCHHHHHHH
AEIGYPQCVLACPTKALSFAEVPVYAKKERKKYLTNFLMKE
HHCCCCEEEEECCHHHHHHHHCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1644755 [H]