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The map label for this gene is yjbJ [H]

Identifier: 78043916

GI number: 78043916

Start: 346674

End: 347252

Strand: Direct

Name: yjbJ [H]

Synonym: CHY_0404

Alternate gene names: 78043916

Gene position: 346674-347252 (Clockwise)

Preceding gene: 78044560

Following gene: 78043172

Centisome position: 14.44

GC content: 44.21

Gene sequence:

>579_bases
ATGATTGATTTAACACCTCTTTTGACTTATTATTATCTTGCTTTACTAACCCAGATGATAGAGCAAATGGAGAGGTTAGA
GGCTCTTTCTTCTTCCGAAATCCCTAAAAGCCTGAATTTTGCCGACCTGGTAGCGGCCAAACAAGAAGCGGCGTTATCCC
CTTCGGAAAACAAGATAGCCGAAATGGTTCGTGAGCTTTCGCAAAAATATGAAGTACCTTATTCGTTAGTAATGGCGGTT
ATTAAGCAGGAATCGAACTTTAATCCCAATGCTACATCCCCCCGGGGAGCCATGGGCCTGATGCAGTTAATGCCCGGAAC
GGCACGGATGTTAGGGGTCGAGAATCCTTATGACCCCAGAGAGAACTTAGAGGGGGGTATTAAATATTTAAAGTCTCTTT
TAGACCGGTTTGGTGATGTGGAACTTGCTCTGGCGGCCTATAATGCCGGACCGGGCAATGTTAGAAAATATAACGGCATT
CCACCCTTTGCGGAAACAAAGGATTATGTCCAAAAGGTGTTAAGGTGGCAGAAGTTTTATCAAGGAGACGAGAGAAATGA
TAGCTTACGAACGGTTTAA

Upstream 100 bases:

>100_bases
GGTAAGGGAAAGCTTTGGCGTACCGACCTTGAGGGGGCTTTAGTTGTCGAAAATTATTAAGTTGTATTAAAATTAAAGAA
AAAAGTACCGGGGTGGAAGG

Downstream 100 bases:

>100_bases
AAATGCCTTAACCCGAGGGGTTTTAAAACCCCTTTACCTTTTTTCCGGAGAAGAGCGGTATTTTAAAGAGCGGCTTTTTG
GGGAAATTAAAAAATATTTA

Product: transglycosylase

Products: 1,6-Anhydrobond [C]

Alternate protein names: NA

Number of amino acids: Translated: 192; Mature: 192

Protein sequence:

>192_residues
MIDLTPLLTYYYLALLTQMIEQMERLEALSSSEIPKSLNFADLVAAKQEAALSPSENKIAEMVRELSQKYEVPYSLVMAV
IKQESNFNPNATSPRGAMGLMQLMPGTARMLGVENPYDPRENLEGGIKYLKSLLDRFGDVELALAAYNAGPGNVRKYNGI
PPFAETKDYVQKVLRWQKFYQGDERNDSLRTV

Sequences:

>Translated_192_residues
MIDLTPLLTYYYLALLTQMIEQMERLEALSSSEIPKSLNFADLVAAKQEAALSPSENKIAEMVRELSQKYEVPYSLVMAV
IKQESNFNPNATSPRGAMGLMQLMPGTARMLGVENPYDPRENLEGGIKYLKSLLDRFGDVELALAAYNAGPGNVRKYNGI
PPFAETKDYVQKVLRWQKFYQGDERNDSLRTV
>Mature_192_residues
MIDLTPLLTYYYLALLTQMIEQMERLEALSSSEIPKSLNFADLVAAKQEAALSPSENKIAEMVRELSQKYEVPYSLVMAV
IKQESNFNPNATSPRGAMGLMQLMPGTARMLGVENPYDPRENLEGGIKYLKSLLDRFGDVELALAAYNAGPGNVRKYNGI
PPFAETKDYVQKVLRWQKFYQGDERNDSLRTV

Specific function: Murein-Degrading Enzyme. Catalyzes The Cleavage Of The Glycosidic Bonds Between N-Acetylmuramic Acid And N- Acetylglucosamine Residues In Peptidoglycan. May Play A Role In Recycling Of Muropeptides During Cell Elongation And/Or Cell Division. [C]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Periplasmic Protein. Tightly Associated With The Murein Sacculus [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transglycosylase slt family [H]

Homologues:

Organism=Escherichia coli, GI87082441, Length=145, Percent_Identity=37.2413793103448, Blast_Score=90, Evalue=1e-19,
Organism=Escherichia coli, GI171474010, Length=97, Percent_Identity=34.020618556701, Blast_Score=64, Evalue=5e-12,
Organism=Escherichia coli, GI87082191, Length=122, Percent_Identity=34.4262295081967, Blast_Score=61, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008258
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 21635; Mature: 21635

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: PS00922 TRANSGLYCOSYLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDLTPLLTYYYLALLTQMIEQMERLEALSSSEIPKSLNFADLVAAKQEAALSPSENKIA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCHHHHH
EMVRELSQKYEVPYSLVMAVIKQESNFNPNATSPRGAMGLMQLMPGTARMLGVENPYDPR
HHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCHHHHHCCCCCCCHH
ENLEGGIKYLKSLLDRFGDVELALAAYNAGPGNVRKYNGIPPFAETKDYVQKVLRWQKFY
HHHHHHHHHHHHHHHHHCCHHHHHEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
QGDERNDSLRTV
CCCCCCCCCCCC
>Mature Secondary Structure
MIDLTPLLTYYYLALLTQMIEQMERLEALSSSEIPKSLNFADLVAAKQEAALSPSENKIA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCHHHHH
EMVRELSQKYEVPYSLVMAVIKQESNFNPNATSPRGAMGLMQLMPGTARMLGVENPYDPR
HHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCHHHHHCCCCCCCHH
ENLEGGIKYLKSLLDRFGDVELALAAYNAGPGNVRKYNGIPPFAETKDYVQKVLRWQKFY
HHHHHHHHHHHHHHHHHCCHHHHHEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
QGDERNDSLRTV
CCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]