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The map label for this gene is ltaE [H]

Identifier: 78043913

GI number: 78043913

Start: 797943

End: 798977

Strand: Direct

Name: ltaE [H]

Synonym: CHY_0904

Alternate gene names: 78043913

Gene position: 797943-798977 (Clockwise)

Preceding gene: 78043108

Following gene: 78044291

Centisome position: 33.23

GC content: 45.6

Gene sequence:

>1035_bases
GTGAAATTTATTGATCTTCGCAGTGATACGGTAACAACTCCTACTCCAAAGATGCGTGAGGCCATGTTTTCTGCCGAGGT
AGGGGATGATGTTTACGGCGATGACCCTACGGTGCGGGAATTAGAAGAACTTGCTGCTCAACGAATTGGCAAGGAAGCGG
CCCTTTTTGTTCCCAGTGGGACCATGGGGAATCAACTGGCTCTTATGACCCATACCCAGCGGGGGGACGAAGTGATTCTG
GGGGAAGATTGCCATATTTTTAAATATGAAGTTGGGGCACCGGCAGTATTATCCGGGGTGCAGCTCCGGGGACTTAAGAC
AAATGGTGGCATGATGGATTTAGAGGAAGTAAAAGCTGCGATACGACCCAACGATATTCACATGCCGAGGACTTCCCTTA
TTTGCCTGGAGAATGCTTACAGTAGCGGAGAAGTAGTGCCCCTTCACTACCTGGAAAAAATCCGGGAAATTGCTGAAAGC
CACGGTCTTAAAATCCACCTGGACGGGGCAAGAATTTTTAATGCCGCTATTTACCTGGGGGTTGATGCCCGGGAAATTGC
CAGGTATGCCGATTCGGTCATGTTTTGTCTCTCCAAAGGTTTATCGGCTCCGGTAGGGTCAATTCTCGCCGGAAGTAGAG
AGTTTGTGGAAAAAGCCAGGAAATACCGGAAATTATTAGGTGGAGGCATGCGGCAGGCGGGAATTTTAGCGGCAGCGGGA
ATGGTAGCTTTGAAAGAAATGGTGGATAGGCTTTGTGAAGACCACGAAAATGCATTATATCTTGCCCAAAAGTTAATTAA
CCTTGGCTTTGAAGTGGATTTAAAAAAGGTGCAGATTAACATGGTGTGGGCCAGGGCTAAATCGCATCTTAATATAGAAG
ATTTACCAGAAAAGCTTTTAGCAGAAGGAATAAAAATAAATCCTCCCATTAATGGGCTGTTTCGTTTCGTAACCCATAAA
GATGTTACGAAAGGTGATATTGATAAATTTGTCGAAATTTTAGCTAAAACTATGCAGGTAGGGGAACAGGTATGA

Upstream 100 bases:

>100_bases
GAAGAGCTGGAAAAAAGGGATACCCGACAGGGGGCAAAATGCGATTATTTAAACGGGAAGGCAGTACAAAAGTTTTTAAG
CTAAGTTAGGGGGGCTTTTG

Downstream 100 bases:

>100_bases
ATTTCAAATGGATAATTGAGAGTTACGGGGAATATTTAGAGCGGATAAGCCACAAGTTTCAGGATATATTAACCGAACAA
AATGGCGCCTTTATTTTGCT

Product: low-specificity L-threonine aldolase

Products: Acetaldehyde; Glycine [C]

Alternate protein names: L-allo-TA; L-allo-threonine acetaldehyde-lyase [H]

Number of amino acids: Translated: 344; Mature: 344

Protein sequence:

>344_residues
MKFIDLRSDTVTTPTPKMREAMFSAEVGDDVYGDDPTVRELEELAAQRIGKEAALFVPSGTMGNQLALMTHTQRGDEVIL
GEDCHIFKYEVGAPAVLSGVQLRGLKTNGGMMDLEEVKAAIRPNDIHMPRTSLICLENAYSSGEVVPLHYLEKIREIAES
HGLKIHLDGARIFNAAIYLGVDAREIARYADSVMFCLSKGLSAPVGSILAGSREFVEKARKYRKLLGGGMRQAGILAAAG
MVALKEMVDRLCEDHENALYLAQKLINLGFEVDLKKVQINMVWARAKSHLNIEDLPEKLLAEGIKINPPINGLFRFVTHK
DVTKGDIDKFVEILAKTMQVGEQV

Sequences:

>Translated_344_residues
MKFIDLRSDTVTTPTPKMREAMFSAEVGDDVYGDDPTVRELEELAAQRIGKEAALFVPSGTMGNQLALMTHTQRGDEVIL
GEDCHIFKYEVGAPAVLSGVQLRGLKTNGGMMDLEEVKAAIRPNDIHMPRTSLICLENAYSSGEVVPLHYLEKIREIAES
HGLKIHLDGARIFNAAIYLGVDAREIARYADSVMFCLSKGLSAPVGSILAGSREFVEKARKYRKLLGGGMRQAGILAAAG
MVALKEMVDRLCEDHENALYLAQKLINLGFEVDLKKVQINMVWARAKSHLNIEDLPEKLLAEGIKINPPINGLFRFVTHK
DVTKGDIDKFVEILAKTMQVGEQV
>Mature_344_residues
MKFIDLRSDTVTTPTPKMREAMFSAEVGDDVYGDDPTVRELEELAAQRIGKEAALFVPSGTMGNQLALMTHTQRGDEVIL
GEDCHIFKYEVGAPAVLSGVQLRGLKTNGGMMDLEEVKAAIRPNDIHMPRTSLICLENAYSSGEVVPLHYLEKIREIAES
HGLKIHLDGARIFNAAIYLGVDAREIARYADSVMFCLSKGLSAPVGSILAGSREFVEKARKYRKLLGGGMRQAGILAAAG
MVALKEMVDRLCEDHENALYLAQKLINLGFEVDLKKVQINMVWARAKSHLNIEDLPEKLLAEGIKINPPINGLFRFVTHK
DVTKGDIDKFVEILAKTMQVGEQV

Specific function: Stereospecifically catalyzes the interconversion of L- allo-threonine and glycine [H]

COG id: COG2008

COG function: function code E; Threonine aldolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the threonine aldolase family [H]

Homologues:

Organism=Escherichia coli, GI1787095, Length=323, Percent_Identity=46.4396284829721, Blast_Score=290, Evalue=7e-80,
Organism=Caenorhabditis elegans, GI193206522, Length=346, Percent_Identity=41.907514450867, Blast_Score=254, Evalue=4e-68,
Organism=Caenorhabditis elegans, GI193206520, Length=346, Percent_Identity=41.907514450867, Blast_Score=254, Evalue=5e-68,
Organism=Caenorhabditis elegans, GI193206524, Length=346, Percent_Identity=41.907514450867, Blast_Score=254, Evalue=6e-68,
Organism=Saccharomyces cerevisiae, GI6320789, Length=334, Percent_Identity=38.622754491018, Blast_Score=197, Evalue=2e-51,
Organism=Drosophila melanogaster, GI21355439, Length=293, Percent_Identity=49.8293515358362, Blast_Score=265, Evalue=5e-71,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001597
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01212 Beta_elim_lyase [H]

EC number: 4.1.2.5 [C]

Molecular weight: Translated: 37920; Mature: 37920

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFIDLRSDTVTTPTPKMREAMFSAEVGDDVYGDDPTVRELEELAAQRIGKEAALFVPSG
CCEEECCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCC
TMGNQLALMTHTQRGDEVILGEDCHIFKYEVGAPAVLSGVQLRGLKTNGGMMDLEEVKAA
CCCCEEEEEEECCCCCEEEECCCCEEEEEECCCCHHHCCCEEEEEECCCCCCCHHHHHHH
IRPNDIHMPRTSLICLENAYSSGEVVPLHYLEKIREIAESHGLKIHLDGARIFNAAIYLG
CCCCCCCCCCHHEEEEECCCCCCCEEHHHHHHHHHHHHHHCCCEEEECCHHEEEEHEEEC
VDAREIARYADSVMFCLSKGLSAPVGSILAGSREFVEKARKYRKLLGGGMRQAGILAAAG
CCHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHH
MVALKEMVDRLCEDHENALYLAQKLINLGFEVDLKKVQINMVWARAKSHLNIEDLPEKLL
HHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCEEEEEEEEEEEEEHHHCCCCHHHHHHHHH
AEGIKINPPINGLFRFVTHKDVTKGDIDKFVEILAKTMQVGEQV
HCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKFIDLRSDTVTTPTPKMREAMFSAEVGDDVYGDDPTVRELEELAAQRIGKEAALFVPSG
CCEEECCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCC
TMGNQLALMTHTQRGDEVILGEDCHIFKYEVGAPAVLSGVQLRGLKTNGGMMDLEEVKAA
CCCCEEEEEEECCCCCEEEECCCCEEEEEECCCCHHHCCCEEEEEECCCCCCCHHHHHHH
IRPNDIHMPRTSLICLENAYSSGEVVPLHYLEKIREIAESHGLKIHLDGARIFNAAIYLG
CCCCCCCCCCHHEEEEECCCCCCCEEHHHHHHHHHHHHHHCCCEEEECCHHEEEEHEEEC
VDAREIARYADSVMFCLSKGLSAPVGSILAGSREFVEKARKYRKLLGGGMRQAGILAAAG
CCHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHH
MVALKEMVDRLCEDHENALYLAQKLINLGFEVDLKKVQINMVWARAKSHLNIEDLPEKLL
HHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCEEEEEEEEEEEEEHHHCCCCHHHHHHHHH
AEGIKINPPINGLFRFVTHKDVTKGDIDKFVEILAKTMQVGEQV
HCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Pyridoxal phosphate [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: 1.7

Km value (mM): 0.22 {L-allo-Thr}} 2.85 {L-Thr}} 0.12 {DL-threo-phenylserine}} 0.24 {DL-erythro-phenylserine}} [C]

Substrates: L-Threonine [C]

Specific reaction: L-Threonine <==> Acetaldehyde + Glycine [C]

General reaction: Elimination of an aldehyde, C-C bond cleavage [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9171400; 9228760 [H]