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The map label for this gene is nucH [H]

Identifier: 78043809

GI number: 78043809

Start: 1514279

End: 1515397

Strand: Direct

Name: nucH [H]

Synonym: CHY_1698

Alternate gene names: 78043809

Gene position: 1514279-1515397 (Clockwise)

Preceding gene: 78043201

Following gene: 78043424

Centisome position: 63.06

GC content: 36.46

Gene sequence:

>1119_bases
ATGAGTTTTAAAATGGATCAACCGGAAAAGTTTCCTTGGTTTAGAAAAATTCCCGGTTTTAGAAGTGGTAAACCTTTAAA
TATGATAATAGCCTCAATCTTTTATCTTACATACCTGGTTATGGCGATTAGCATAATTCGTGAATCATCTAATAATTTTT
GGGAAACTGTTGCAATTTTTTCATTAGTCTTTTTGTTACCAATAAGTTTAATTGCCTCAATATTTTTAGCTTTTAAGCGT
GATCCTCTTTGGAAAAAGTGGTTAGCTGGAGCAGGAATTGCTTTTTTAGTTTTTTTTGTTAGCGTAATAAATTCCCCAAC
TTCGCAAGCACCGAATAATAATGCCTCTAAGCCTACTTCAAAACAAGAAGTTGTAGCTACCTCCCAGCAAAATCAATCCA
CTACTGATTCAATAAGCTCTGAAAAAACACCTACCATAGCCCCTCAAAGCCAAAATTCACAAACAAATGAAAATACTCAA
CAGTCCTCCCAAGATCAGCAAGCCCCTATCCCTTTAATTGCCGCTAAAGTTACAAAAGTAGTTGATGGTGATACTATTTA
TGTTCGACTTCCCAATGGTTCTGAAGAAAAGGTTAGGTTTATCGGAGTTGACACTCCTGAAAGCACAATTCAACATGAGC
CATATGGCAATGAAGCTTCAAATTATACAAAGTCTAAGTTGGCCGGTAAAACTGTTTACCTTGAAAAAGACGTTAGCGAA
AGAGACAAATATGGAAGATTGCTCCGTTACATTTGGTTAGCAAAGCCCAAAGAAATAAGTGAAAGCGAAATTAGGTCAAA
AATGTTTAATGCCATTCTCTTGCTCGGAGGTTATGCCCAAGTTGCCACTTATCCTCCGGATGTAAAATATGTAGATTACT
TTGTCAAGTTTCAACAAGAAGCAAGAGAAGCCGAAAAAGGACTTTGGGGAATTTCACCAACTCAAGAAGAAGATATTAGC
TTAGCTATTCAGTATGTCGGAAATAAAAATAGTAGGAAATTCCATTACGAGGACTGCCGCTGGGCTAAAAAAATTGCACC
TTCTAATAGGGTTTACTTTAAATCGAGAGAAGCAGCTATAAATGCCGGATATGTTCCTTGTAAAGTATGTAATCCATAG

Upstream 100 bases:

>100_bases
GAATGTCACCTATTCATGTAAAACAATTAATTGAGATACAAGCTACTCTTTCAAAAATGATCAAACCCAATGAGTAAATT
TTAAAGGAGGTTTTTTTATT

Downstream 100 bases:

>100_bases
CCTTAAGGCAAAATCCTTGGGGGTATTTAGATGAGTTCTATAGAAAAAATTAAACAATCATTAAAAGAACAAAAAAGTTT
ATTAATTGGATTATGTTTTT

Product: prophage LambdaCh01 nuclease domain-containing protein

Products: NA

Alternate protein names: TNase; Micrococcal nuclease; Staphylococcal nuclease [H]

Number of amino acids: Translated: 372; Mature: 371

Protein sequence:

>372_residues
MSFKMDQPEKFPWFRKIPGFRSGKPLNMIIASIFYLTYLVMAISIIRESSNNFWETVAIFSLVFLLPISLIASIFLAFKR
DPLWKKWLAGAGIAFLVFFVSVINSPTSQAPNNNASKPTSKQEVVATSQQNQSTTDSISSEKTPTIAPQSQNSQTNENTQ
QSSQDQQAPIPLIAAKVTKVVDGDTIYVRLPNGSEEKVRFIGVDTPESTIQHEPYGNEASNYTKSKLAGKTVYLEKDVSE
RDKYGRLLRYIWLAKPKEISESEIRSKMFNAILLLGGYAQVATYPPDVKYVDYFVKFQQEAREAEKGLWGISPTQEEDIS
LAIQYVGNKNSRKFHYEDCRWAKKIAPSNRVYFKSREAAINAGYVPCKVCNP

Sequences:

>Translated_372_residues
MSFKMDQPEKFPWFRKIPGFRSGKPLNMIIASIFYLTYLVMAISIIRESSNNFWETVAIFSLVFLLPISLIASIFLAFKR
DPLWKKWLAGAGIAFLVFFVSVINSPTSQAPNNNASKPTSKQEVVATSQQNQSTTDSISSEKTPTIAPQSQNSQTNENTQ
QSSQDQQAPIPLIAAKVTKVVDGDTIYVRLPNGSEEKVRFIGVDTPESTIQHEPYGNEASNYTKSKLAGKTVYLEKDVSE
RDKYGRLLRYIWLAKPKEISESEIRSKMFNAILLLGGYAQVATYPPDVKYVDYFVKFQQEAREAEKGLWGISPTQEEDIS
LAIQYVGNKNSRKFHYEDCRWAKKIAPSNRVYFKSREAAINAGYVPCKVCNP
>Mature_371_residues
SFKMDQPEKFPWFRKIPGFRSGKPLNMIIASIFYLTYLVMAISIIRESSNNFWETVAIFSLVFLLPISLIASIFLAFKRD
PLWKKWLAGAGIAFLVFFVSVINSPTSQAPNNNASKPTSKQEVVATSQQNQSTTDSISSEKTPTIAPQSQNSQTNENTQQ
SSQDQQAPIPLIAAKVTKVVDGDTIYVRLPNGSEEKVRFIGVDTPESTIQHEPYGNEASNYTKSKLAGKTVYLEKDVSER
DKYGRLLRYIWLAKPKEISESEIRSKMFNAILLLGGYAQVATYPPDVKYVDYFVKFQQEAREAEKGLWGISPTQEEDISL
AIQYVGNKNSRKFHYEDCRWAKKIAPSNRVYFKSREAAINAGYVPCKVCNP

Specific function: Enzyme that catalyzes the hydrolysis of both DNA and RNA at the 5'-position of the phosphodiester bond [H]

COG id: COG1525

COG function: function code L; Micrococcal nuclease (thermonuclease) homologs

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thermonuclease family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016071
- InterPro:   IPR006021
- InterPro:   IPR002071 [H]

Pfam domain/function: PF00565 SNase [H]

EC number: =3.1.31.1 [H]

Molecular weight: Translated: 41994; Mature: 41863

Theoretical pI: Translated: 9.57; Mature: 9.57

Prosite motif: PS50830 TNASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFKMDQPEKFPWFRKIPGFRSGKPLNMIIASIFYLTYLVMAISIIRESSNNFWETVAIF
CCCCCCCCCCCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
SLVFLLPISLIASIFLAFKRDPLWKKWLAGAGIAFLVFFVSVINSPTSQAPNNNASKPTS
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
KQEVVATSQQNQSTTDSISSEKTPTIAPQSQNSQTNENTQQSSQDQQAPIPLIAAKVTKV
HHHHHHHCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCHHHHHHHHHH
VDGDTIYVRLPNGSEEKVRFIGVDTPESTIQHEPYGNEASNYTKSKLAGKTVYLEKDVSE
CCCCEEEEECCCCCCCCEEEEECCCCHHHHCCCCCCCCHHCHHHHHHCCCEEEEECCHHH
RDKYGRLLRYIWLAKPKEISESEIRSKMFNAILLLGGYAQVATYPPDVKYVDYFVKFQQE
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCHHHCCCCCCHHHHHHHHHHHHH
AREAEKGLWGISPTQEEDISLAIQYVGNKNSRKFHYEDCRWAKKIAPSNRVYFKSREAAI
HHHHHCCCCCCCCCCCCCCEEEEEEECCCCCCEEEHHHHHHHHHCCCCCCEEEECCHHEE
NAGYVPCKVCNP
CCCCCCEEECCC
>Mature Secondary Structure 
SFKMDQPEKFPWFRKIPGFRSGKPLNMIIASIFYLTYLVMAISIIRESSNNFWETVAIF
CCCCCCCCCCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
SLVFLLPISLIASIFLAFKRDPLWKKWLAGAGIAFLVFFVSVINSPTSQAPNNNASKPTS
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
KQEVVATSQQNQSTTDSISSEKTPTIAPQSQNSQTNENTQQSSQDQQAPIPLIAAKVTKV
HHHHHHHCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCHHHHHHHHHH
VDGDTIYVRLPNGSEEKVRFIGVDTPESTIQHEPYGNEASNYTKSKLAGKTVYLEKDVSE
CCCCEEEEECCCCCCCCEEEEECCCCHHHHCCCCCCCCHHCHHHHHHCCCEEEEECCHHH
RDKYGRLLRYIWLAKPKEISESEIRSKMFNAILLLGGYAQVATYPPDVKYVDYFVKFQQE
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCHHHCCCCCCHHHHHHHHHHHHH
AREAEKGLWGISPTQEEDISLAIQYVGNKNSRKFHYEDCRWAKKIAPSNRVYFKSREAAI
HHHHHCCCCCCCCCCCCCCEEEEEEECCCCCCEEEHHHHHHHHHCCCCCCEEEECCHHEE
NAGYVPCKVCNP
CCCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8045422 [H]