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The map label for this gene is abfD [H]

Identifier: 78043633

GI number: 78043633

Start: 1169432

End: 1170907

Strand: Direct

Name: abfD [H]

Synonym: CHY_1314

Alternate gene names: 78043633

Gene position: 1169432-1170907 (Clockwise)

Preceding gene: 78044403

Following gene: 78044533

Centisome position: 48.7

GC content: 38.41

Gene sequence:

>1476_bases
ATGCCTTTAAAAACAGTAAAGGAGTACCTTAATAGCTTAAGAGCGATGAAGTTTGAAGTTTATTTTAGGGGAGAGCGGAT
AGAAAATCCAGTAGATCACCCCGTAATACGTCCTTCAGTTAATGCTGTAGCTATGACCTATGCTTTAGCGCATAATAGTA
AGTACAAAGATCTTGCTACTGCTTATTCACCTTTTGTTGATAAGTATGTAAATCGTTTTACTCATATTCATCAAAGCACT
GATGATTTGTTAAAAAAAGTTGAGTTGTTAAGAACAATTGGGCAATTAACTGGGGTTTGTTTCCAACGTTGTGTTGGCAT
GGACGGTTTAAATGCCGTTTATGCTGTAACGTATGAAATAGATGAAAAATATGGGACCCAATATCACCAAAGGTTTATAA
ATTATTTAAAATATGTGCAAGAAAATGATTTAGTTGTAGATGGAGCTATGACAGATCCGAAAGGTGACAGGGGGTTAAGG
CCTTCTGAACAGGAAGATAAGGATCTTTATTTGCGGGTTGTTAAGGAAACAGAAGAAGGGATAGTTGTTCGGGGTGCTAA
GTTCCACCAAACAGGAGCTATTAACTCCCATGAAATATTAGTCATGCCAACAATCTCCTTAAAAGGAGAAGAAGAAAAAC
CCTACGCAGTATCTTTTGCTGTACCTTCGGATTCTCCAGGAATAGTCTATGTATATGGACGTCAGCCATCGGATTTAAGA
AGTTTGTACGATGCTCCTGAGGATTGTGGCAATCCTAAATTTGCGGGGCAAGAAGCTGTGGTAATTTTTAATGATGTTTT
TGTTCCCTGGGAAAGGGTATTTATGTGCGGTGAAGTGGAGTTTGCTGGAAATCTTGTCGAGTATTTTGCAAGTTACCATA
GGCAAAGCTATGGTGGATGCAAAGTTGGAGTTGGTGATGTTTTAATTGGAGCTGCTGCTAATATTGCGGAGTTTAATGGG
GTAGATAAAGCTTCGCATATTAAAGATAAGATTGTTGAAATGATTCATTTAAATGAAACGATATTTTCTTGTGGTGTTGC
TTGCTCTTGTTTGGGGTATAAAACAAGTGCAGGTAACTATATGGTTGATAGATTATTGGCAAATGTTTGCAAACAAAATG
TAACACGGATGCCCTATGAAATTGCAAGGCTACTGCAGGATATAGCTGGCGGACTTTTTGTGACAATGCCGTCATGGAAA
GACTTAGAAAATGGTAAGGTTGGAGAATTGTTGCAGAAATATTTGGTCGGCAAGAAAGGCGTAAAAGTAAAAGATCGGGT
AAAACTAATGAGATTAATAGAAAACTTAACATTAGGACGATCTGCTGTTGGGTATTTACCGGAATCGATGCATGGTGCCG
GTTCACCTCAAGCTCAAAGAATTATGATTACCAGGCTTGCTGATATAGAGAAAAAGAAAAAGTTTGCCTTGCGGTTAGCT
GGAATTGGTGAGCAAGAAAATGAGCGATTGGAGTGA

Upstream 100 bases:

>100_bases
AGTGAAAATATTATAAAATTAAATGAACTGGCATTAGAAAAAGGTAGGGAAGCAGCATTGCAATTATAAAATAAAAAATT
TTGAGGGGGAAGTGTTTATT

Downstream 100 bases:

>100_bases
ACATTGATTGGGATCTGTACGCATAATAGCTTATATAGATAAGACGGTAGTAAAAATTGTAGGATTTAAAATTGATAGAA
TTAATTTAATGGAAATTGAA

Product: gamma-aminobutyrate metabolism dehydratase/isomerase

Products: NA

Alternate protein names: 4-BUDH; Gamma-aminobutyrate metabolism dehydratase/isomerase [H]

Number of amino acids: Translated: 491; Mature: 490

Protein sequence:

>491_residues
MPLKTVKEYLNSLRAMKFEVYFRGERIENPVDHPVIRPSVNAVAMTYALAHNSKYKDLATAYSPFVDKYVNRFTHIHQST
DDLLKKVELLRTIGQLTGVCFQRCVGMDGLNAVYAVTYEIDEKYGTQYHQRFINYLKYVQENDLVVDGAMTDPKGDRGLR
PSEQEDKDLYLRVVKETEEGIVVRGAKFHQTGAINSHEILVMPTISLKGEEEKPYAVSFAVPSDSPGIVYVYGRQPSDLR
SLYDAPEDCGNPKFAGQEAVVIFNDVFVPWERVFMCGEVEFAGNLVEYFASYHRQSYGGCKVGVGDVLIGAAANIAEFNG
VDKASHIKDKIVEMIHLNETIFSCGVACSCLGYKTSAGNYMVDRLLANVCKQNVTRMPYEIARLLQDIAGGLFVTMPSWK
DLENGKVGELLQKYLVGKKGVKVKDRVKLMRLIENLTLGRSAVGYLPESMHGAGSPQAQRIMITRLADIEKKKKFALRLA
GIGEQENERLE

Sequences:

>Translated_491_residues
MPLKTVKEYLNSLRAMKFEVYFRGERIENPVDHPVIRPSVNAVAMTYALAHNSKYKDLATAYSPFVDKYVNRFTHIHQST
DDLLKKVELLRTIGQLTGVCFQRCVGMDGLNAVYAVTYEIDEKYGTQYHQRFINYLKYVQENDLVVDGAMTDPKGDRGLR
PSEQEDKDLYLRVVKETEEGIVVRGAKFHQTGAINSHEILVMPTISLKGEEEKPYAVSFAVPSDSPGIVYVYGRQPSDLR
SLYDAPEDCGNPKFAGQEAVVIFNDVFVPWERVFMCGEVEFAGNLVEYFASYHRQSYGGCKVGVGDVLIGAAANIAEFNG
VDKASHIKDKIVEMIHLNETIFSCGVACSCLGYKTSAGNYMVDRLLANVCKQNVTRMPYEIARLLQDIAGGLFVTMPSWK
DLENGKVGELLQKYLVGKKGVKVKDRVKLMRLIENLTLGRSAVGYLPESMHGAGSPQAQRIMITRLADIEKKKKFALRLA
GIGEQENERLE
>Mature_490_residues
PLKTVKEYLNSLRAMKFEVYFRGERIENPVDHPVIRPSVNAVAMTYALAHNSKYKDLATAYSPFVDKYVNRFTHIHQSTD
DLLKKVELLRTIGQLTGVCFQRCVGMDGLNAVYAVTYEIDEKYGTQYHQRFINYLKYVQENDLVVDGAMTDPKGDRGLRP
SEQEDKDLYLRVVKETEEGIVVRGAKFHQTGAINSHEILVMPTISLKGEEEKPYAVSFAVPSDSPGIVYVYGRQPSDLRS
LYDAPEDCGNPKFAGQEAVVIFNDVFVPWERVFMCGEVEFAGNLVEYFASYHRQSYGGCKVGVGDVLIGAAANIAEFNGV
DKASHIKDKIVEMIHLNETIFSCGVACSCLGYKTSAGNYMVDRLLANVCKQNVTRMPYEIARLLQDIAGGLFVTMPSWKD
LENGKVGELLQKYLVGKKGVKVKDRVKLMRLIENLTLGRSAVGYLPESMHGAGSPQAQRIMITRLADIEKKKKFALRLAG
IGEQENERLE

Specific function: Catalyzes the reversible conversion of 4-hydroxybutyryl- CoA to crotonyl-CoA. The mechanism of the reaction seems to go through three steps:(1) the FAD-dependent oxidation of 4- hydroxybutyryl-CoA to 4-hydroxycrotonyl-CoA; (2) the hydroxyl group is substi

COG id: COG2368

COG function: function code Q; Aromatic ring hydroxylase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006091
- InterPro:   IPR009075
- InterPro:   IPR009100
- InterPro:   IPR004925 [H]

Pfam domain/function: PF03241 HpaB [H]

EC number: =4.2.1.120; =5.3.3.3 [H]

Molecular weight: Translated: 55034; Mature: 54903

Theoretical pI: Translated: 7.28; Mature: 7.28

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLKTVKEYLNSLRAMKFEVYFRGERIENPVDHPVIRPSVNAVAMTYALAHNSKYKDLAT
CCHHHHHHHHHHHHHEEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHH
AYSPFVDKYVNRFTHIHQSTDDLLKKVELLRTIGQLTGVCFQRCVGMDGLNAVYAVTYEI
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEE
DEKYGTQYHQRFINYLKYVQENDLVVDGAMTDPKGDRGLRPSEQEDKDLYLRVVKETEEG
CHHHCHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCC
IVVRGAKFHQTGAINSHEILVMPTISLKGEEEKPYAVSFAVPSDSPGIVYVYGRQPSDLR
EEEECCEEECCCCCCCCCEEEEEEEECCCCCCCCEEEEEECCCCCCCEEEEECCCCHHHH
SLYDAPEDCGNPKFAGQEAVVIFNDVFVPWERVFMCGEVEFAGNLVEYFASYHRQSYGGC
HHHCCHHHCCCCCCCCCCEEEEEECCCCCHHHHHEECCHHHHHHHHHHHHHHHHCCCCCC
KVGVGDVLIGAAANIAEFNGVDKASHIKDKIVEMIHLNETIFSCGVACSCLGYKTSAGNY
EECHHHHHHHCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHH
MVDRLLANVCKQNVTRMPYEIARLLQDIAGGLFVTMPSWKDLENGKVGELLQKYLVGKKG
HHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCEEEECCCCCCCCCCCHHHHHHHHHCCCCC
VKVKDRVKLMRLIENLTLGRSAVGYLPESMHGAGSPQAQRIMITRLADIEKKKKFALRLA
CCHHHHHHHHHHHHHHHCCCHHHHCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHEE
GIGEQENERLE
CCCCCCCCCCC
>Mature Secondary Structure 
PLKTVKEYLNSLRAMKFEVYFRGERIENPVDHPVIRPSVNAVAMTYALAHNSKYKDLAT
CHHHHHHHHHHHHHEEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHH
AYSPFVDKYVNRFTHIHQSTDDLLKKVELLRTIGQLTGVCFQRCVGMDGLNAVYAVTYEI
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEE
DEKYGTQYHQRFINYLKYVQENDLVVDGAMTDPKGDRGLRPSEQEDKDLYLRVVKETEEG
CHHHCHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCC
IVVRGAKFHQTGAINSHEILVMPTISLKGEEEKPYAVSFAVPSDSPGIVYVYGRQPSDLR
EEEECCEEECCCCCCCCCEEEEEEEECCCCCCCCEEEEEECCCCCCCEEEEECCCCHHHH
SLYDAPEDCGNPKFAGQEAVVIFNDVFVPWERVFMCGEVEFAGNLVEYFASYHRQSYGGC
HHHCCHHHCCCCCCCCCCEEEEEECCCCCHHHHHEECCHHHHHHHHHHHHHHHHCCCCCC
KVGVGDVLIGAAANIAEFNGVDKASHIKDKIVEMIHLNETIFSCGVACSCLGYKTSAGNY
EECHHHHHHHCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHH
MVDRLLANVCKQNVTRMPYEIARLLQDIAGGLFVTMPSWKDLENGKVGELLQKYLVGKKG
HHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCEEEECCCCCCCCCCCHHHHHHHHHCCCCC
VKVKDRVKLMRLIENLTLGRSAVGYLPESMHGAGSPQAQRIMITRLADIEKKKKFALRLA
CCHHHHHHHHHHHHHHHCCCHHHHCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHEE
GIGEQENERLE
CCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11041350; 8344309 [H]