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The map label for this gene is dut [H]

Identifier: 78043524

GI number: 78043524

Start: 263865

End: 264305

Strand: Direct

Name: dut [H]

Synonym: CHY_0300

Alternate gene names: 78043524

Gene position: 263865-264305 (Clockwise)

Preceding gene: 78043735

Following gene: 78043927

Centisome position: 10.99

GC content: 46.71

Gene sequence:

>441_bases
ATGCGCGGATTTAAGGTTGTAAGTTTTTTTGAGGACAAAGGAATTAACATCCCACAGCGAAAGACCAAAGCCAGTGCCGG
TTATGATTTGGAAGCAGCGATTGACGTGGTTTTAAACCCGGGTGAGGTAAAACTGGTACCTACCGGTGTTAAAGCTTATA
TGCCCGAAGATGAGGTACTCCTCATTTACATTAGAAGTAGCCTTGCGGTGAAAAAAGGTTTGCTTTTGGCCAACGGCGTA
GGGGTAGTAGATGCGGATTATTACGATAACCCGGAAAACGAAGGGCATATCATGATACCTTTGTTTAACCCCGGAAAAGA
ACCGGTAACGATCAGGAAAGGGGAGCGGATAGCCCAGGGGATTTTTACCAGATATTTAAAGGCCGATGATGACGAGGCTC
TGGGCCAAAGAACGGGCGGGTTTGGTTCTACCGGTCAGTAA

Upstream 100 bases:

>100_bases
TTTAAAATTCCTAATTTACAATTAAAAAGTTTTTTTAAAAACGAAAGGCCTTTATAATAATAAAAAGGGAGAAACTAAAC
AGGTGAATATGGAGGAGAAT

Downstream 100 bases:

>100_bases
AAAATAACAGTAAAATTTCTTTATTAACAACTTGCCTTCAAACGAAAAATGTGTATAATAGTAAGAGCGAGGAATAAAGA
AGTGCGCCCGTAGCTCAGCG

Product: deoxyuridine 5`-triphosphate nucleotidohydrolase

Products: NA

Alternate protein names: dUTPase; dUTP pyrophosphatase [H]

Number of amino acids: Translated: 146; Mature: 146

Protein sequence:

>146_residues
MRGFKVVSFFEDKGINIPQRKTKASAGYDLEAAIDVVLNPGEVKLVPTGVKAYMPEDEVLLIYIRSSLAVKKGLLLANGV
GVVDADYYDNPENEGHIMIPLFNPGKEPVTIRKGERIAQGIFTRYLKADDDEALGQRTGGFGSTGQ

Sequences:

>Translated_146_residues
MRGFKVVSFFEDKGINIPQRKTKASAGYDLEAAIDVVLNPGEVKLVPTGVKAYMPEDEVLLIYIRSSLAVKKGLLLANGV
GVVDADYYDNPENEGHIMIPLFNPGKEPVTIRKGERIAQGIFTRYLKADDDEALGQRTGGFGSTGQ
>Mature_146_residues
MRGFKVVSFFEDKGINIPQRKTKASAGYDLEAAIDVVLNPGEVKLVPTGVKAYMPEDEVLLIYIRSSLAVKKGLLLANGV
GVVDADYYDNPENEGHIMIPLFNPGKEPVTIRKGERIAQGIFTRYLKADDDEALGQRTGGFGSTGQ

Specific function: This enzyme is involved in nucleotide metabolism:it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA [H]

COG id: COG0756

COG function: function code F; dUTPase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dUTPase family [H]

Homologues:

Organism=Homo sapiens, GI70906444, Length=136, Percent_Identity=37.5, Blast_Score=72, Evalue=2e-13,
Organism=Homo sapiens, GI4503423, Length=136, Percent_Identity=37.5, Blast_Score=71, Evalue=3e-13,
Organism=Homo sapiens, GI70906441, Length=136, Percent_Identity=37.5, Blast_Score=70, Evalue=8e-13,
Organism=Escherichia coli, GI1790071, Length=138, Percent_Identity=37.6811594202899, Blast_Score=86, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6319729, Length=136, Percent_Identity=33.0882352941176, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008180 [H]

Pfam domain/function: PF00692 dUTPase [H]

EC number: =3.6.1.23 [H]

Molecular weight: Translated: 15902; Mature: 15902

Theoretical pI: Translated: 5.27; Mature: 5.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRGFKVVSFFEDKGINIPQRKTKASAGYDLEAAIDVVLNPGEVKLVPTGVKAYMPEDEVL
CCCCEEEEEECCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCEEEEECCCEEECCCCCEE
LIYIRSSLAVKKGLLLANGVGVVDADYYDNPENEGHIMIPLFNPGKEPVTIRKGERIAQG
EEEEECCHHHHCCEEEECCCCEEECCCCCCCCCCCEEEEEEECCCCCCEEEECCCHHHHH
IFTRYLKADDDEALGQRTGGFGSTGQ
HHHHHHCCCCCHHHHHCCCCCCCCCC
>Mature Secondary Structure
MRGFKVVSFFEDKGINIPQRKTKASAGYDLEAAIDVVLNPGEVKLVPTGVKAYMPEDEVL
CCCCEEEEEECCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCEEEEECCCEEECCCCCEE
LIYIRSSLAVKKGLLLANGVGVVDADYYDNPENEGHIMIPLFNPGKEPVTIRKGERIAQG
EEEEECCHHHHCCEEEECCCCEEECCCCCCCCCCCEEEEEEECCCCCCEEEECCCHHHHH
IFTRYLKADDDEALGQRTGGFGSTGQ
HHHHHHCCCCCHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11337471 [H]