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The map label for this gene is nasB [H]

Identifier: 78043443

GI number: 78043443

Start: 651652

End: 652836

Strand: Direct

Name: nasB [H]

Synonym: CHY_0737

Alternate gene names: 78043443

Gene position: 651652-652836 (Clockwise)

Preceding gene: 78044052

Following gene: 78042883

Centisome position: 27.13

GC content: 42.62

Gene sequence:

>1185_bases
ATGAAATACTTAATTATTGGAAACAGTGCTGCAGGAATTTTTGCGGCAGAAAGTTTAAGAAAGTTGGACCCAGCGGGCGA
AATCACCGTTTTAACCGATGAGCCGTACGAGGTTTACGGGCGCTGTTTAACATCTTACTTTATTGCCGGGGACATAGTTG
AGGAGCAAATCTTTATCCGTCCCAAGGATTTTTACGAAAAAAACCGGATAAATCTTAAAAAAGGTGAAAAAGTAGTAAGA
ATTGATTTTAATGAAAAAAAAGTCATAACTTTCAAAAATTCTTACCAATATGACCGGCTTTTAATTGCGAGCGGTGCAAG
GGCCAAAAAGCTTTCGCTCCCGGGAAGTAATTTACCCGGAGTTTTCACCTTACGAACCCTGGATGATGCCAAAAATATCC
TGGATTATTCCCGCAAAGCTGAACAAGCTGTTATAGTGGGGGGAGGACTGGTTAGCTTAAAAGGGGCTTACGGTTTATTA
AAGAGAGGGGTTAAAGTTACCGTTGTGGTGGCCTCCCGCCAGATTTTATCCCAGGTTCTGGATTACGAAGCGGCCGGGTT
GGTGCAGCAAAACTTAGAAAAACAGGGGATGAAATTTCTTTTAGGGGAAGATGTTTTGGAGTTTTTAGGAGAAGACAAAA
TTTTCGAAGTAAAACTTACTAACGGACAGGTTATAAAAGCTGATTTAGTTTTAATTGGTAAAGGTGTTACCCCCAATGTC
GATTTTTTACCGGAACCGGAGAAATTTTTGGAAGGTATACCGGTGGACCAGTATTTAAGGACTCCCTGGGAGGGTGTTTG
GGCAGCGGGGGATGTGGCGAAAACTTTTGATGTTGCTCATGGTAAATACAGAGTTAATGCTCTCTGGCCAATTGCCGCCG
AGCAGGGAAGGGTGGCAGCAATGAATATGGTCGGACAAAATTATGTGTATCAAGGTTCCATAGGCATGAATTCCCTGGAG
TTTTTTGGGTACAATACCATTGCAGCGGGAATTACCCGGCAGGAAGAAGGTTATACGGTTATCAAACGAAGACAGGGAGC
CAACTATTTAAAGCTCGTATTGGAGGGCGAAAAACTAAAAGGGTACATTTTAAGCGGGGAGGTGCGGGGAGCCGGAAAAC
TTACCCAGCTTATCCGTTCCGGTAGCAAAATAAAGCAATTTCCCTCCTTTTTAAAAATATTTTAA

Upstream 100 bases:

>100_bases
AAGTTTTACGTGGAAACGGATCCTTATAAAGCAGCAGCCGGTATTATTGAACATATTAAAGCTAAACGGGCTTTGTTAAA
CCTTTAGGGGGTTAACCTTA

Downstream 100 bases:

>100_bases
AAACAATAAATTTCTGGTAAAATGTAGATAAAAATCAAAGGAGGGAAGATAATGAACCAAAAAACCTTAGAAAATTTAAT
GGCCGCTTTTGCCGGAGAAT

Product: nitrite reductase

Products: Oxidized Ferredoxin; NADH; cis-3-(carboxyethyl)-3,5-cyclohexadiene-1,2-diol; NAD [C]

Alternate protein names: NA

Number of amino acids: Translated: 394; Mature: 394

Protein sequence:

>394_residues
MKYLIIGNSAAGIFAAESLRKLDPAGEITVLTDEPYEVYGRCLTSYFIAGDIVEEQIFIRPKDFYEKNRINLKKGEKVVR
IDFNEKKVITFKNSYQYDRLLIASGARAKKLSLPGSNLPGVFTLRTLDDAKNILDYSRKAEQAVIVGGGLVSLKGAYGLL
KRGVKVTVVVASRQILSQVLDYEAAGLVQQNLEKQGMKFLLGEDVLEFLGEDKIFEVKLTNGQVIKADLVLIGKGVTPNV
DFLPEPEKFLEGIPVDQYLRTPWEGVWAAGDVAKTFDVAHGKYRVNALWPIAAEQGRVAAMNMVGQNYVYQGSIGMNSLE
FFGYNTIAAGITRQEEGYTVIKRRQGANYLKLVLEGEKLKGYILSGEVRGAGKLTQLIRSGSKIKQFPSFLKIF

Sequences:

>Translated_394_residues
MKYLIIGNSAAGIFAAESLRKLDPAGEITVLTDEPYEVYGRCLTSYFIAGDIVEEQIFIRPKDFYEKNRINLKKGEKVVR
IDFNEKKVITFKNSYQYDRLLIASGARAKKLSLPGSNLPGVFTLRTLDDAKNILDYSRKAEQAVIVGGGLVSLKGAYGLL
KRGVKVTVVVASRQILSQVLDYEAAGLVQQNLEKQGMKFLLGEDVLEFLGEDKIFEVKLTNGQVIKADLVLIGKGVTPNV
DFLPEPEKFLEGIPVDQYLRTPWEGVWAAGDVAKTFDVAHGKYRVNALWPIAAEQGRVAAMNMVGQNYVYQGSIGMNSLE
FFGYNTIAAGITRQEEGYTVIKRRQGANYLKLVLEGEKLKGYILSGEVRGAGKLTQLIRSGSKIKQFPSFLKIF
>Mature_394_residues
MKYLIIGNSAAGIFAAESLRKLDPAGEITVLTDEPYEVYGRCLTSYFIAGDIVEEQIFIRPKDFYEKNRINLKKGEKVVR
IDFNEKKVITFKNSYQYDRLLIASGARAKKLSLPGSNLPGVFTLRTLDDAKNILDYSRKAEQAVIVGGGLVSLKGAYGLL
KRGVKVTVVVASRQILSQVLDYEAAGLVQQNLEKQGMKFLLGEDVLEFLGEDKIFEVKLTNGQVIKADLVLIGKGVTPNV
DFLPEPEKFLEGIPVDQYLRTPWEGVWAAGDVAKTFDVAHGKYRVNALWPIAAEQGRVAAMNMVGQNYVYQGSIGMNSLE
FFGYNTIAAGITRQEEGYTVIKRRQGANYLKLVLEGEKLKGYILSGEVRGAGKLTQLIRSGSKIKQFPSFLKIF

Specific function: Required for nitrate assimilation [H]

COG id: COG0446

COG function: function code R; Uncharacterized NAD(FAD)-dependent dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI65787454, Length=336, Percent_Identity=33.0357142857143, Blast_Score=144, Evalue=1e-34,
Organism=Homo sapiens, GI21389617, Length=336, Percent_Identity=32.7380952380952, Blast_Score=144, Evalue=1e-34,
Organism=Homo sapiens, GI226437568, Length=336, Percent_Identity=32.7380952380952, Blast_Score=144, Evalue=2e-34,
Organism=Homo sapiens, GI22202629, Length=366, Percent_Identity=23.4972677595628, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI4757732, Length=366, Percent_Identity=23.4972677595628, Blast_Score=74, Evalue=2e-13,
Organism=Escherichia coli, GI1788892, Length=279, Percent_Identity=28.673835125448, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1789065, Length=365, Percent_Identity=24.6575342465753, Blast_Score=88, Evalue=8e-19,
Organism=Escherichia coli, GI1789765, Length=410, Percent_Identity=23.9024390243902, Blast_Score=85, Evalue=9e-18,
Organism=Caenorhabditis elegans, GI17559934, Length=283, Percent_Identity=28.9752650176678, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24585130, Length=312, Percent_Identity=24.6794871794872, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI281359715, Length=311, Percent_Identity=27.9742765273312, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI281359713, Length=311, Percent_Identity=27.9742765273312, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24639250, Length=311, Percent_Identity=27.9742765273312, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI18543267, Length=311, Percent_Identity=27.9742765273312, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24639252, Length=311, Percent_Identity=27.9742765273312, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24639257, Length=311, Percent_Identity=27.9742765273312, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24581020, Length=352, Percent_Identity=25.2840909090909, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI28573993, Length=352, Percent_Identity=25.2840909090909, Blast_Score=84, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007419
- InterPro:   IPR013027
- InterPro:   IPR005117
- InterPro:   IPR012744
- InterPro:   IPR017121
- InterPro:   IPR006067
- InterPro:   IPR001327 [H]

Pfam domain/function: PF04324 Fer2_BFD; PF01077 NIR_SIR; PF03460 NIR_SIR_ferr; PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.18.1.3 [C]

Molecular weight: Translated: 43700; Mature: 43700

Theoretical pI: Translated: 9.66; Mature: 9.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYLIIGNSAAGIFAAESLRKLDPAGEITVLTDEPYEVYGRCLTSYFIAGDIVEEQIFIR
CEEEEEECCCCCHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHCCCEEEEC
PKDFYEKNRINLKKGEKVVRIDFNEKKVITFKNSYQYDRLLIASGARAKKLSLPGSNLPG
CHHHHHCCCCCCCCCCEEEEEECCCCEEEEEECCCCCCEEEEECCCCEEEEECCCCCCCC
VFTLRTLDDAKNILDYSRKAEQAVIVGGGLVSLKGAYGLLKRGVKVTVVVASRQILSQVL
EEEEEEHHHHHHHHHHHHCCCEEEEEECCEEEECHHHHHHHCCCEEEEEEECHHHHHHHH
DYEAAGLVQQNLEKQGMKFLLGEDVLEFLGEDKIFEVKLTNGQVIKADLVLIGKGVTPNV
CHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCEEEEEECCCEEEEEEEEEEECCCCCCC
DFLPEPEKFLEGIPVDQYLRTPWEGVWAAGDVAKTFDVAHGKYRVNALWPIAAEQGRVAA
CCCCCHHHHHCCCCHHHHHCCCCCCEEECCCHHHHHHCCCCEEEEEEEEEEECCCCCEEE
MNMVGQNYVYQGSIGMNSLEFFGYNTIAAGITRQEEGYTVIKRRQGANYLKLVLEGEKLK
EEECCCCEEEECCCCCCCEEECCCHHHHHCCEECCCCCEEEEECCCCCEEEEEEECCCEE
GYILSGEVRGAGKLTQLIRSGSKIKQFPSFLKIF
EEEEECCCCCCHHHHHHHHCCCHHHHHHHHHHCC
>Mature Secondary Structure
MKYLIIGNSAAGIFAAESLRKLDPAGEITVLTDEPYEVYGRCLTSYFIAGDIVEEQIFIR
CEEEEEECCCCCHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHCCCEEEEC
PKDFYEKNRINLKKGEKVVRIDFNEKKVITFKNSYQYDRLLIASGARAKKLSLPGSNLPG
CHHHHHCCCCCCCCCCEEEEEECCCCEEEEEECCCCCCEEEEECCCCEEEEECCCCCCCC
VFTLRTLDDAKNILDYSRKAEQAVIVGGGLVSLKGAYGLLKRGVKVTVVVASRQILSQVL
EEEEEEHHHHHHHHHHHHCCCEEEEEECCEEEECHHHHHHHCCCEEEEEEECHHHHHHHH
DYEAAGLVQQNLEKQGMKFLLGEDVLEFLGEDKIFEVKLTNGQVIKADLVLIGKGVTPNV
CHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCEEEEEECCCEEEEEEEEEEECCCCCCC
DFLPEPEKFLEGIPVDQYLRTPWEGVWAAGDVAKTFDVAHGKYRVNALWPIAAEQGRVAA
CCCCCHHHHHCCCCHHHHHCCCCCCEEECCCHHHHHHCCCCEEEEEEEEEEECCCCCEEE
MNMVGQNYVYQGSIGMNSLEFFGYNTIAAGITRQEEGYTVIKRRQGANYLKLVLEGEKLK
EEECCCCEEEECCCCCCCEEECCCHHHHHCCEECCCCCEEEEECCCCCEEEEEEECCCEE
GYILSGEVRGAGKLTQLIRSGSKIKQFPSFLKIF
EEEEECCCCCCHHHHHHHHCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Reduced Ferredoxin; NAD(+); 3-phenylpropionate; NADH; Proton; O2 [C]

Specific reaction: Reduced Ferredoxin + NAD(+) = Oxidized Ferredoxin + NADH. 3-phenylpropionate + NADH + Proton + O2 = cis-3-(carboxyethyl)-3,5-cyclohexadiene-1,2-diol + NAD [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7868621; 8969502; 9384377 [H]