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The map label for this gene is pfkA2 [H]

Identifier: 78043322

GI number: 78043322

Start: 1207920

End: 1209005

Strand: Direct

Name: pfkA2 [H]

Synonym: CHY_1349

Alternate gene names: 78043322

Gene position: 1207920-1209005 (Clockwise)

Preceding gene: 78042630

Following gene: 78044080

Centisome position: 50.3

GC content: 44.84

Gene sequence:

>1086_bases
ATGGCTGTGGGTATTTTAACCGGTGGTGGTGATTGCCCGGGCTTAAATGCAGTAATTCGGGCTTTAGTGAAAACTCTTCA
ACAAAAGCTTCCCGAAGAGAAAATAATCGGCTTTTTAGATGGTTTTCGCGGGGCAATTGAAAACCGAAGTGTGCCTCTAA
CAACGAAAGACGTGGTAGGTTTACTCCCTAAAGGCGGAACGATTTTGGGCACTTCCAACCGGGATAATCCTTTTAAATTT
CCCGTATCTGTTAACGGCACCTTAGAATTTCACGATGTTAGCGATACTGTAATCGAAAATTTACATAAAAACAATATTGA
GGTGTTATTCGTCATTGGGGGTGATGGTTCTCTTTCTATTGCCCACGAACTCTCCCAAAAGGGTGTAAAGGTTATAGGAA
TCCCAAAAACTATAGATAATGACCTGGATGCTACCGATTTTACCTTTGGCTTTGACAGTGCGGTAAGGACCGCAACCAAA
GCTCTTGATATGCTTCACACCACTGCTGAATCCCACCACCGGATCATGGTATTGGAGGTTATGGGACGGTATGCCGGCTG
GATTGCCCTATATTCGGGACTGGCCGGAGGTGCAGATGTTATTTTAATTCCCGAAATTCCCTTTAACTGGGATGCCGTTT
GCCGAAAAATTCAGCAAAGAAAAGCCGAAGGAAAGCATTTTTCCATAGTTGTAGTTGCTGAAGGAGCCTATCCCGAAGGT
GGGGAAAGGGTAATTGCAAGATATGTTAAGGAAAGTACCGATCCGGCCCGTTTGGGGGGAATTGGCCAGGTAGTAGCTGA
CCATTTAAGCCGCTGTACGGGCTTGGAAAGCAGGGTAACGGTTTTAGGTCACCTGCAAAGGGGTGGAGGTCCTACACCCT
TTGACCGCATCCTGGCGACTCGCTTTGGGGTTGCTGCAGTAAATACATGGCTTCAGGGTAATTATAATGTCATGGTTGCC
TTTTTAGGGGGAAGGGTAACTACGGTGCCTTTAAGCGAAGGAATAAAAAAATTAAAATTAGTCCCCGTTGATTCAGAATT
AATTGTAACCGCTAAAGCAATAGGTATAAGTTTTGGAGATGAATGA

Upstream 100 bases:

>100_bases
AGATCTGCGAACTTTGCAAAAAAAAGTTAAAAAAAGAAGCGGAAGATAGCAGTAAACCGGTAAAACCCATAGGATAATTA
CAAAGAAGGAGGGATACGAA

Downstream 100 bases:

>100_bases
TTGATATGTAAATAAATGTTTACTATGCGCACACATTTATTTACAGTTTTAAGTTCTATTTTTGCTCTTTTAGCCCTAAT
TTTAAAATGGCATTAATTTT

Product: phosphofructokinase

Products: NA

Alternate protein names: Phosphofructokinase 2; Phosphohexokinase 2 [H]

Number of amino acids: Translated: 361; Mature: 360

Protein sequence:

>361_residues
MAVGILTGGGDCPGLNAVIRALVKTLQQKLPEEKIIGFLDGFRGAIENRSVPLTTKDVVGLLPKGGTILGTSNRDNPFKF
PVSVNGTLEFHDVSDTVIENLHKNNIEVLFVIGGDGSLSIAHELSQKGVKVIGIPKTIDNDLDATDFTFGFDSAVRTATK
ALDMLHTTAESHHRIMVLEVMGRYAGWIALYSGLAGGADVILIPEIPFNWDAVCRKIQQRKAEGKHFSIVVVAEGAYPEG
GERVIARYVKESTDPARLGGIGQVVADHLSRCTGLESRVTVLGHLQRGGGPTPFDRILATRFGVAAVNTWLQGNYNVMVA
FLGGRVTTVPLSEGIKKLKLVPVDSELIVTAKAIGISFGDE

Sequences:

>Translated_361_residues
MAVGILTGGGDCPGLNAVIRALVKTLQQKLPEEKIIGFLDGFRGAIENRSVPLTTKDVVGLLPKGGTILGTSNRDNPFKF
PVSVNGTLEFHDVSDTVIENLHKNNIEVLFVIGGDGSLSIAHELSQKGVKVIGIPKTIDNDLDATDFTFGFDSAVRTATK
ALDMLHTTAESHHRIMVLEVMGRYAGWIALYSGLAGGADVILIPEIPFNWDAVCRKIQQRKAEGKHFSIVVVAEGAYPEG
GERVIARYVKESTDPARLGGIGQVVADHLSRCTGLESRVTVLGHLQRGGGPTPFDRILATRFGVAAVNTWLQGNYNVMVA
FLGGRVTTVPLSEGIKKLKLVPVDSELIVTAKAIGISFGDE
>Mature_360_residues
AVGILTGGGDCPGLNAVIRALVKTLQQKLPEEKIIGFLDGFRGAIENRSVPLTTKDVVGLLPKGGTILGTSNRDNPFKFP
VSVNGTLEFHDVSDTVIENLHKNNIEVLFVIGGDGSLSIAHELSQKGVKVIGIPKTIDNDLDATDFTFGFDSAVRTATKA
LDMLHTTAESHHRIMVLEVMGRYAGWIALYSGLAGGADVILIPEIPFNWDAVCRKIQQRKAEGKHFSIVVVAEGAYPEGG
ERVIARYVKESTDPARLGGIGQVVADHLSRCTGLESRVTVLGHLQRGGGPTPFDRILATRFGVAAVNTWLQGNYNVMVAF
LGGRVTTVPLSEGIKKLKLVPVDSELIVTAKAIGISFGDE

Specific function: Key control step of glycolysis. [C]

COG id: COG0205

COG function: function code G; 6-phosphofructokinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphofructokinase family [H]

Homologues:

Organism=Homo sapiens, GI11321601, Length=366, Percent_Identity=34.6994535519126, Blast_Score=176, Evalue=3e-44,
Organism=Homo sapiens, GI266453619, Length=371, Percent_Identity=33.4231805929919, Blast_Score=174, Evalue=1e-43,
Organism=Homo sapiens, GI266453768, Length=371, Percent_Identity=33.4231805929919, Blast_Score=174, Evalue=1e-43,
Organism=Homo sapiens, GI266453748, Length=371, Percent_Identity=33.4231805929919, Blast_Score=174, Evalue=1e-43,
Organism=Homo sapiens, GI4505749, Length=371, Percent_Identity=33.4231805929919, Blast_Score=174, Evalue=1e-43,
Organism=Homo sapiens, GI48762920, Length=374, Percent_Identity=33.1550802139037, Blast_Score=172, Evalue=6e-43,
Organism=Escherichia coli, GI1790350, Length=314, Percent_Identity=39.4904458598726, Blast_Score=211, Evalue=5e-56,
Organism=Caenorhabditis elegans, GI25147584, Length=370, Percent_Identity=32.7027027027027, Blast_Score=165, Evalue=4e-41,
Organism=Caenorhabditis elegans, GI17558788, Length=354, Percent_Identity=31.0734463276836, Blast_Score=144, Evalue=5e-35,
Organism=Caenorhabditis elegans, GI72003601, Length=201, Percent_Identity=35.8208955223881, Blast_Score=126, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6323861, Length=377, Percent_Identity=32.8912466843501, Blast_Score=166, Evalue=7e-42,
Organism=Saccharomyces cerevisiae, GI6321679, Length=388, Percent_Identity=31.4432989690722, Blast_Score=157, Evalue=3e-39,
Organism=Drosophila melanogaster, GI24652337, Length=370, Percent_Identity=31.8918918918919, Blast_Score=171, Evalue=1e-42,
Organism=Drosophila melanogaster, GI17647809, Length=370, Percent_Identity=31.8918918918919, Blast_Score=170, Evalue=2e-42,
Organism=Drosophila melanogaster, GI28573326, Length=370, Percent_Identity=31.3513513513514, Blast_Score=168, Evalue=4e-42,

Paralogues:

None

Copy number: 3981 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 950 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012003
- InterPro:   IPR012829
- InterPro:   IPR022953
- InterPro:   IPR015912
- InterPro:   IPR000023 [H]

Pfam domain/function: PF00365 PFK [H]

EC number: =2.7.1.11 [H]

Molecular weight: Translated: 38617; Mature: 38486

Theoretical pI: Translated: 7.02; Mature: 7.02

Prosite motif: PS00433 PHOSPHOFRUCTOKINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVGILTGGGDCPGLNAVIRALVKTLQQKLPEEKIIGFLDGFRGAIENRSVPLTTKDVVG
CEEEEEECCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHH
LLPKGGTILGTSNRDNPFKFPVSVNGTLEFHDVSDTVIENLHKNNIEVLFVIGGDGSLSI
CCCCCCEEEECCCCCCCEEEEEEECCEEEEECCHHHHHHHHCCCCEEEEEEECCCCCCHH
AHELSQKGVKVIGIPKTIDNDLDATDFTFGFDSAVRTATKALDMLHTTAESHHRIMVLEV
HHHHHHCCCEEEECCCCCCCCCCCCCEECCCHHHHHHHHHHHHHHHHHHHCCCCEEEHHH
MGRYAGWIALYSGLAGGADVILIPEIPFNWDAVCRKIQQRKAEGKHFSIVVVAEGAYPEG
HHHHHHHHHHHHCCCCCCCEEEECCCCCCHHHHHHHHHHHHCCCCEEEEEEEECCCCCCC
GERVIARYVKESTDPARLGGIGQVVADHLSRCTGLESRVTVLGHLQRGGGPTPFDRILAT
HHHHHHHHHHCCCCCHHHCCHHHHHHHHHHHHCCCCHHHHHEEEHHCCCCCCHHHHHHHH
RFGVAAVNTWLQGNYNVMVAFLGGRVTTVPLSEGIKKLKLVPVDSELIVTAKAIGISFGD
HHHHHHHHHHHCCCCEEEEEEECCEEEEEEHHHCHHEEEEEECCCCEEEEEEEEEEECCC
E
C
>Mature Secondary Structure 
AVGILTGGGDCPGLNAVIRALVKTLQQKLPEEKIIGFLDGFRGAIENRSVPLTTKDVVG
EEEEEECCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHH
LLPKGGTILGTSNRDNPFKFPVSVNGTLEFHDVSDTVIENLHKNNIEVLFVIGGDGSLSI
CCCCCCEEEECCCCCCCEEEEEEECCEEEEECCHHHHHHHHCCCCEEEEEEECCCCCCHH
AHELSQKGVKVIGIPKTIDNDLDATDFTFGFDSAVRTATKALDMLHTTAESHHRIMVLEV
HHHHHHCCCEEEECCCCCCCCCCCCCEECCCHHHHHHHHHHHHHHHHHHHCCCCEEEHHH
MGRYAGWIALYSGLAGGADVILIPEIPFNWDAVCRKIQQRKAEGKHFSIVVVAEGAYPEG
HHHHHHHHHHHHCCCCCCCEEEECCCCCCHHHHHHHHHHHHCCCCEEEEEEEECCCCCCC
GERVIARYVKESTDPARLGGIGQVVADHLSRCTGLESRVTVLGHLQRGGGPTPFDRILAT
HHHHHHHHHHCCCCCHHHCCHHHHHHHHHHHHCCCCHHHHHEEEHHCCCCCCHHHHHHHH
RFGVAAVNTWLQGNYNVMVAFLGGRVTTVPLSEGIKKLKLVPVDSELIVTAKAIGISFGD
HHHHHHHHHHHCCCCEEEEEEECCEEEEEEHHHCHHEEEEEECCCCEEEEEEEEEEECCC
E
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11792842 [H]