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The map label for this gene is moaA

Identifier: 78043321

GI number: 78043321

Start: 711751

End: 712716

Strand: Direct

Name: moaA

Synonym: CHY_0802

Alternate gene names: 78043321

Gene position: 711751-712716 (Clockwise)

Preceding gene: 78044014

Following gene: 78044091

Centisome position: 29.64

GC content: 43.06

Gene sequence:

>966_bases
ATGCTTGATTCTTTTAACCGGGAAATAAACTATTTGCGGATTTCGGTTACCGACAAATGTAACCTTAACTGTTTTTACTG
TAAACCCGGAAATTTTCAAGAATTTTCGCCGGGAGATATTTTAACCTTTGAGGAAATTTTAGAAGTAGTCCGGGCCTTTT
TACCCTTGGGTTTAAAAAAAGTACGGATTACCGGCGGCGAGCCATTGGTTAGAAAAAATCTTTTATATTTAATTGAAAAT
ATTGCAGCTTTACCGGGTATTGATGATTTAGCCCTCACTACCAACGGTATCTTGTTTCCCCGCTACGCTCGAGATTTAAA
AAGTGCTGGGCTTTCGCGGGTAAACTTTAGCCTTGATAGCTTGAATCCGGACAAATTTCGCTCGATTACCGGCGGCGGCG
AGTTAAAAAATGTGCTGGAAGCCATTAATCTTGCCCTGGAATTGGACCTTACTCCGGTAAAAATAAATACGGTTTTATTA
CGAGGTATTAATTTAGACGAAATCGATGCTTTCGTGGATTTTATCTTTCGGTATCCGGTGCACTGGCGGTTTATTGAGCT
TATGCCATTAAATGACCGGGAAAAGTGGCAGCGGCAATTTGTTTCGGTGAAAGAAATTGTGGAAATCATCTCAGCCAAGT
ACAAACTAATTCCGGGGAAAACGGTGGGTGGCGCAGGGCCGGCCCGCTATTATGAAGTGCCGGAAGCATTGGGAACGATT
GGGGTAATTTCTCCCCTGTCCAATCATTTTTGCGAATACTGTAATCGCCTCAGGCTTACCTCTACCGGTAAGTTAAGGCT
TTGTTTGGCCAAAAACCAGGAAGTTGATATAAAAAGCCTTTTACGGTCCGGGATTTCGCAAGAGGAGTTGACCGGAGTTT
TGCGGGAGGCAATTTTAAGAAAACCCAGGGCCCATGAGTTTGGCGATAAGGACTTTTTAGGAACAATGGCCAACATAGGA
GGTTAA

Upstream 100 bases:

>100_bases
AAGGGTACTACCGGGAAACCGGGGTGCCTCCCGTTTTGGAAAGGAGTTATCAAAGCGCCTTTTTAAAACGGGGCGCTTTT
AATTTTAGGAGGCTAAAGAA

Downstream 100 bases:

>100_bases
TGCGATGGAGCTTACCCACTTTAATGAAGAAGGACGGGCGCGAATGGTAGAGGTGACCGAAAAGCAAGAAACGGTACGGA
CAGCGGTAGCTGCCGGAGAA

Product: molybdenum cofactor biosynthesis protein MoaA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 321; Mature: 321

Protein sequence:

>321_residues
MLDSFNREINYLRISVTDKCNLNCFYCKPGNFQEFSPGDILTFEEILEVVRAFLPLGLKKVRITGGEPLVRKNLLYLIEN
IAALPGIDDLALTTNGILFPRYARDLKSAGLSRVNFSLDSLNPDKFRSITGGGELKNVLEAINLALELDLTPVKINTVLL
RGINLDEIDAFVDFIFRYPVHWRFIELMPLNDREKWQRQFVSVKEIVEIISAKYKLIPGKTVGGAGPARYYEVPEALGTI
GVISPLSNHFCEYCNRLRLTSTGKLRLCLAKNQEVDIKSLLRSGISQEELTGVLREAILRKPRAHEFGDKDFLGTMANIG
G

Sequences:

>Translated_321_residues
MLDSFNREINYLRISVTDKCNLNCFYCKPGNFQEFSPGDILTFEEILEVVRAFLPLGLKKVRITGGEPLVRKNLLYLIEN
IAALPGIDDLALTTNGILFPRYARDLKSAGLSRVNFSLDSLNPDKFRSITGGGELKNVLEAINLALELDLTPVKINTVLL
RGINLDEIDAFVDFIFRYPVHWRFIELMPLNDREKWQRQFVSVKEIVEIISAKYKLIPGKTVGGAGPARYYEVPEALGTI
GVISPLSNHFCEYCNRLRLTSTGKLRLCLAKNQEVDIKSLLRSGISQEELTGVLREAILRKPRAHEFGDKDFLGTMANIG
G
>Mature_321_residues
MLDSFNREINYLRISVTDKCNLNCFYCKPGNFQEFSPGDILTFEEILEVVRAFLPLGLKKVRITGGEPLVRKNLLYLIEN
IAALPGIDDLALTTNGILFPRYARDLKSAGLSRVNFSLDSLNPDKFRSITGGGELKNVLEAINLALELDLTPVKINTVLL
RGINLDEIDAFVDFIFRYPVHWRFIELMPLNDREKWQRQFVSVKEIVEIISAKYKLIPGKTVGGAGPARYYEVPEALGTI
GVISPLSNHFCEYCNRLRLTSTGKLRLCLAKNQEVDIKSLLRSGISQEELTGVLREAILRKPRAHEFGDKDFLGTMANIG
G

Specific function: Together with moaC, is involved in the conversion of a guanosine derivative (5'-GTP) into molybdopterin precursor Z

COG id: COG2896

COG function: function code H; Molybdenum cofactor biosynthesis enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. MoaA family

Homologues:

Organism=Homo sapiens, GI116734668, Length=307, Percent_Identity=40.0651465798046, Blast_Score=233, Evalue=1e-61,
Organism=Homo sapiens, GI22027536, Length=307, Percent_Identity=40.0651465798046, Blast_Score=233, Evalue=2e-61,
Organism=Escherichia coli, GI1786999, Length=307, Percent_Identity=35.5048859934853, Blast_Score=202, Evalue=2e-53,
Organism=Caenorhabditis elegans, GI32566331, Length=308, Percent_Identity=37.3376623376623, Blast_Score=213, Evalue=8e-56,
Organism=Caenorhabditis elegans, GI17561210, Length=312, Percent_Identity=37.8205128205128, Blast_Score=213, Evalue=1e-55,
Organism=Caenorhabditis elegans, GI32566329, Length=308, Percent_Identity=37.3376623376623, Blast_Score=213, Evalue=2e-55,
Organism=Drosophila melanogaster, GI28574490, Length=307, Percent_Identity=38.1107491856677, Blast_Score=223, Evalue=2e-58,
Organism=Drosophila melanogaster, GI28574488, Length=307, Percent_Identity=38.1107491856677, Blast_Score=221, Evalue=4e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MOAA_CARHZ (Q3ADX8)

Other databases:

- EMBL:   CP000141
- RefSeq:   YP_359656.1
- HSSP:   P69848
- ProteinModelPortal:   Q3ADX8
- SMR:   Q3ADX8
- STRING:   Q3ADX8
- GeneID:   3728646
- GenomeReviews:   CP000141_GR
- KEGG:   chy:CHY_0802
- NMPDR:   fig|246194.3.peg.989
- TIGR:   CHY_0802
- eggNOG:   COG2896
- HOGENOM:   HBG529885
- OMA:   PLVRRNM
- BioCyc:   CHYD246194:CHY_0802-MONOMER
- HAMAP:   MF_01225_B
- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR013483
- InterPro:   IPR000385
- InterPro:   IPR010505
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00729
- TIGRFAMs:   TIGR02666

Pfam domain/function: PF06463 Mob_synth_C; PF04055 Radical_SAM

EC number: NA

Molecular weight: Translated: 36094; Mature: 36094

Theoretical pI: Translated: 8.10; Mature: 8.10

Prosite motif: PS01305 MOAA_NIFB_PQQE

Important sites: BINDING 13-13 BINDING 26-26 BINDING 62-62 BINDING 66-66 BINDING 93-93 BINDING 117-117 BINDING 154-154 BINDING 188-188

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDSFNREINYLRISVTDKCNLNCFYCKPGNFQEFSPGDILTFEEILEVVRAFLPLGLKK
CCCCCCCCEEEEEEEECCCCCCEEEEECCCCCCCCCCCCEECHHHHHHHHHHHHCCCCEE
VRITGGEPLVRKNLLYLIENIAALPGIDDLALTTNGILFPRYARDLKSAGLSRVNFSLDS
EEEECCCCHHHHHHHHHHHHHHHCCCCCCCEECCCCEECHHHHHHHHHCCCCEEEEECCC
LNPDKFRSITGGGELKNVLEAINLALELDLTPVKINTVLLRGINLDEIDAFVDFIFRYPV
CCHHHHHCCCCCHHHHHHHHHHHHHEEECCCEEEEEEEEEECCCHHHHHHHHHHHHHCCC
HWRFIELMPLNDREKWQRQFVSVKEIVEIISAKYKLIPGKTVGGAGPARYYEVPEALGTI
CEEEEEEEECCCHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCCCCCHHHHHHHHHHHHH
GVISPLSNHFCEYCNRLRLTSTGKLRLCLAKNQEVDIKSLLRSGISQEELTGVLREAILR
HHHHHHHHHHHHHHCCEEECCCCCEEEEEECCCCCCHHHHHHCCCCHHHHHHHHHHHHHC
KPRAHEFGDKDFLGTMANIGG
CCCCCCCCCHHHHHHHHHCCC
>Mature Secondary Structure
MLDSFNREINYLRISVTDKCNLNCFYCKPGNFQEFSPGDILTFEEILEVVRAFLPLGLKK
CCCCCCCCEEEEEEEECCCCCCEEEEECCCCCCCCCCCCEECHHHHHHHHHHHHCCCCEE
VRITGGEPLVRKNLLYLIENIAALPGIDDLALTTNGILFPRYARDLKSAGLSRVNFSLDS
EEEECCCCHHHHHHHHHHHHHHHCCCCCCCEECCCCEECHHHHHHHHHCCCCEEEEECCC
LNPDKFRSITGGGELKNVLEAINLALELDLTPVKINTVLLRGINLDEIDAFVDFIFRYPV
CCHHHHHCCCCCHHHHHHHHHHHHHEEECCCEEEEEEEEEECCCHHHHHHHHHHHHHCCC
HWRFIELMPLNDREKWQRQFVSVKEIVEIISAKYKLIPGKTVGGAGPARYYEVPEALGTI
CEEEEEEEECCCHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCCCCCHHHHHHHHHHHHH
GVISPLSNHFCEYCNRLRLTSTGKLRLCLAKNQEVDIKSLLRSGISQEELTGVLREAILR
HHHHHHHHHHHHHHCCEEECCCCCEEEEEECCCCCCHHHHHHCCCCHHHHHHHHHHHHHC
KPRAHEFGDKDFLGTMANIGG
CCCCCCCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA