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The map label for this gene is fumA [H]

Identifier: 78043076

GI number: 78043076

Start: 51846

End: 52400

Strand: Direct

Name: fumA [H]

Synonym: CHY_0062

Alternate gene names: 78043076

Gene position: 51846-52400 (Clockwise)

Preceding gene: 78044230

Following gene: 78044723

Centisome position: 2.16

GC content: 40.9

Gene sequence:

>555_bases
ATGAAAGAGCCTATCCGTATTACACTTCCTTTAACTGAAGAAAAGGTGCGTAGTCTTAAAGCAGGTGATAGTGTCTTAAT
TACTGGTGTAGTTTATACTGGCCGGGATGCTGCTCACAAGCGCATGGTAGAAGCGTTGGACCGTGGAGAAGAACTACCTA
CTGATTTAAAGGATCAAGTGATTTATTATGTGGGACCAACTCCTGCAAAGCCAGGTAAACCTATAGGGTCAGCTGGGCCT
ACCACTAGTGGTCGGATGGATGCATATACGCCTCGACTTTTAGAAAAAGGACTAAGAGGAATGATTGGTAAAGGATACCG
TTCGCCTGAAGTAATTGAGGCAATTAAAAAATATGGAGCAGTATATTTTGTTGCTATTGGGGGAGCAGCTGCATTGATTG
CCAAAAGTATTAAGAGTTATGAAGTAATTGCTTACGAAGATTTAGGGCCTGAAGCACATGCCAAAATCTATGTAGAAGAT
TTTCCGGCGATTGTAGCTATTGATTGTGAAGGAAACAATATCTACGAAATTGGACCAAAAAAATATAAGAGGTGA

Upstream 100 bases:

>100_bases
CAACCCACATTGCAGGTTTGCCAGTGGCAATTAATATAAATTGTCACGCCACCAGGCATGCTGAAATCGAACTTTAAAGA
CTCTATAAGGGGGAAAAAAG

Downstream 100 bases:

>100_bases
TTAAATGCTTAAGTACGACGTACTCGTAATAGGCAGTGGAGGAGCGGGAATGCAGGCTGCTTTAGTAGCAGCTAAGTCGG
GAGAATTAAATGTTGCTCTT

Product: fumarate hydratase

Products: NA

Alternate protein names: Fumarase [H]

Number of amino acids: Translated: 184; Mature: 184

Protein sequence:

>184_residues
MKEPIRITLPLTEEKVRSLKAGDSVLITGVVYTGRDAAHKRMVEALDRGEELPTDLKDQVIYYVGPTPAKPGKPIGSAGP
TTSGRMDAYTPRLLEKGLRGMIGKGYRSPEVIEAIKKYGAVYFVAIGGAAALIAKSIKSYEVIAYEDLGPEAHAKIYVED
FPAIVAIDCEGNNIYEIGPKKYKR

Sequences:

>Translated_184_residues
MKEPIRITLPLTEEKVRSLKAGDSVLITGVVYTGRDAAHKRMVEALDRGEELPTDLKDQVIYYVGPTPAKPGKPIGSAGP
TTSGRMDAYTPRLLEKGLRGMIGKGYRSPEVIEAIKKYGAVYFVAIGGAAALIAKSIKSYEVIAYEDLGPEAHAKIYVED
FPAIVAIDCEGNNIYEIGPKKYKR
>Mature_184_residues
MKEPIRITLPLTEEKVRSLKAGDSVLITGVVYTGRDAAHKRMVEALDRGEELPTDLKDQVIYYVGPTPAKPGKPIGSAGP
TTSGRMDAYTPRLLEKGLRGMIGKGYRSPEVIEAIKKYGAVYFVAIGGAAALIAKSIKSYEVIAYEDLGPEAHAKIYVED
FPAIVAIDCEGNNIYEIGPKKYKR

Specific function: It functions as an aerobic enzyme in the citric acid cycle. It accounts for about 80% of the fumarase activity when the bacteria grows aerobically [H]

COG id: COG1838

COG function: function code C; Tartrate dehydratase beta subunit/Fumarate hydratase class I, C-terminal domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I fumarase family [H]

Homologues:

Organism=Escherichia coli, GI1790564, Length=152, Percent_Identity=47.3684210526316, Blast_Score=136, Evalue=7e-34,
Organism=Escherichia coli, GI1787897, Length=180, Percent_Identity=42.2222222222222, Blast_Score=134, Evalue=3e-33,
Organism=Escherichia coli, GI1789443, Length=173, Percent_Identity=34.1040462427746, Blast_Score=101, Evalue=3e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004646
- InterPro:   IPR004647
- InterPro:   IPR011167
- InterPro:   IPR020557 [H]

Pfam domain/function: PF05681 Fumerase; PF05683 Fumerase_C [H]

EC number: =4.2.1.2 [H]

Molecular weight: Translated: 20029; Mature: 20029

Theoretical pI: Translated: 9.10; Mature: 9.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKEPIRITLPLTEEKVRSLKAGDSVLITGVVYTGRDAAHKRMVEALDRGEELPTDLKDQV
CCCCEEEEECCCHHHHHHHHCCCEEEEEEEEEECCHHHHHHHHHHHHCCCCCCCCCCCCE
IYYVGPTPAKPGKPIGSAGPTTSGRMDAYTPRLLEKGLRGMIGKGYRSPEVIEAIKKYGA
EEEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHCCC
VYFVAIGGAAALIAKSIKSYEVIAYEDLGPEAHAKIYVEDFPAIVAIDCEGNNIYEIGPK
EEEEEECCHHHHHHHHHCCEEEEEEECCCCCCCEEEEEECCCEEEEEECCCCCEEEECCH
KYKR
HCCC
>Mature Secondary Structure
MKEPIRITLPLTEEKVRSLKAGDSVLITGVVYTGRDAAHKRMVEALDRGEELPTDLKDQV
CCCCEEEEECCCHHHHHHHHCCCEEEEEEEEEECCHHHHHHHHHHHHCCCCCCCCCCCCE
IYYVGPTPAKPGKPIGSAGPTTSGRMDAYTPRLLEKGLRGMIGKGYRSPEVIEAIKKYGA
EEEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHCCC
VYFVAIGGAAALIAKSIKSYEVIAYEDLGPEAHAKIYVEDFPAIVAIDCEGNNIYEIGPK
EEEEEECCHHHHHHHHHCCEEEEEEECCCCCCCEEEEEECCCEEEEEECCCCCEEEECCH
KYKR
HCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677609; 1879695 [H]