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The map label for this gene is nrfA [H]

Identifier: 78042886

GI number: 78042886

Start: 208895

End: 210160

Strand: Direct

Name: nrfA [H]

Synonym: CHY_0243

Alternate gene names: 78042886

Gene position: 208895-210160 (Clockwise)

Preceding gene: 78042788

Following gene: 78044097

Centisome position: 8.7

GC content: 43.92

Gene sequence:

>1266_bases
TTGACCCGCGGTATAACAATTGGAATTATTATAGTTGCTCTAACTTTGCTTGCGGGAACTTTTGTGGCCTACATTACTGC
CAAAAACGATGTTCCCTATAGAACTTTGACAACCGGTTTTAAGAAAGATGAGATTAAACCCGAAAAGTACGGCCAAAAAT
TTCCGGTTGAATATAAGTATTACTTAAAAAATAAAGAAATGGCTCTGGCTCCCAGTAAGTACGGAGGCTCGGTAAAAAGA
GACCACCTCAAGGAATTTCCTTATATGAAAACCTTATGGGCGGGCTTTGCCTTCAGTAAAGACTATACTGAAGATCGGGG
TCATGTTTATGCTCTGGAAGATGTTAAGGAAACCAAAAGGATTTCCGAAAAAACCGCCTTAGCCTGTATGACCTGTAAAT
CTTCCCAGGTACCGGTTTATATGGACAAATGGGGAGTAGATAACTTTTATAAAGCCAGATTTAGAGAAAAACAAAATCTT
TTTACGATACCGATTGGCTGTTATGACTGTCATGATGCTCAGACCATGAAGTTACGCATTACCCGGCCGGCGTTAAAAGA
TGCGTTAAAACGAATTGGCCGGGATCCGGAGAAATTTACGGAGAAAGAACTTAAGAATTATATCTGCGCCCAGTGTCATG
TAACTTATTATATTGACAAGGCAACAAACCGGGTAATCTTTCCCTGGGATGGGGGAGTAGATCCGGAAGATATGTACCGG
CATTATGAAAAAAGAAATTTTTATGAATGGGTACATCCTTTAAGCGGCACTAAAATGCTGAAATCCCGCCATCCGGAGTT
TGAAATGTTTTCGGGAAGCGTGCACAGTGCGGTGGGGCTTACCTGCGTTGATTGTCATATGCCGTACGTCTATGAAGGCG
GCAAAAAATATTCCAGCCACTGGTGGACTTCTCCGTTAAGAACTTTAGAGCAGTCCTGCCGCAAGTGCCACCGGGAAGAG
GAGGCTAAGCTTAGAGAGCGGGTCTTTTACATCCAGGACCGAACTTATGACTTATTAAACCGGGCGGGGAAAGCCAATGA
AGAAGCAATTAAAGCTATAAATGAAGCAGTATATTCTCCGGGGGCAGATAAAGCTTTAATTGAAAAGGCGCGGAAGCTTC
ACCGGGAGGCCCAGTGGTACTGGGATTATGTCAGCAGCGAAAACTCTATGGGCTTTCATAACCCGGAAAAAGCCCTGACT
ACGCTGGGTAAATCCATCGACCTTGCCCTTAGAGCCAAAGCTACCGCCCTCCAGGCCAAAAAGTAA

Upstream 100 bases:

>100_bases
TGTCATCAGGGGGTCACGGAAAAGCTTTTGGGGGTGTCCCAGCGGTGCACCTTTTGTCACCGGCAGGTATTTCACTGAAA
ACTGTTAGGAGGGGATAATT

Downstream 100 bases:

>100_bases
CAGAAGGGACGGTTCTGAGAATGACCATTTTCCCGAAAAGAAGCAGGGAAAATGGTGGCTGACAGAACCGTCCCTCCAGT
TAACCGTGGATGAGGCGGGG

Product: cytochrome c552

Products: NA

Alternate protein names: Ammonia-forming cytochrome c nitrite reductase; Cytochrome c nitrite reductase [H]

Number of amino acids: Translated: 421; Mature: 420

Protein sequence:

>421_residues
MTRGITIGIIIVALTLLAGTFVAYITAKNDVPYRTLTTGFKKDEIKPEKYGQKFPVEYKYYLKNKEMALAPSKYGGSVKR
DHLKEFPYMKTLWAGFAFSKDYTEDRGHVYALEDVKETKRISEKTALACMTCKSSQVPVYMDKWGVDNFYKARFREKQNL
FTIPIGCYDCHDAQTMKLRITRPALKDALKRIGRDPEKFTEKELKNYICAQCHVTYYIDKATNRVIFPWDGGVDPEDMYR
HYEKRNFYEWVHPLSGTKMLKSRHPEFEMFSGSVHSAVGLTCVDCHMPYVYEGGKKYSSHWWTSPLRTLEQSCRKCHREE
EAKLRERVFYIQDRTYDLLNRAGKANEEAIKAINEAVYSPGADKALIEKARKLHREAQWYWDYVSSENSMGFHNPEKALT
TLGKSIDLALRAKATALQAKK

Sequences:

>Translated_421_residues
MTRGITIGIIIVALTLLAGTFVAYITAKNDVPYRTLTTGFKKDEIKPEKYGQKFPVEYKYYLKNKEMALAPSKYGGSVKR
DHLKEFPYMKTLWAGFAFSKDYTEDRGHVYALEDVKETKRISEKTALACMTCKSSQVPVYMDKWGVDNFYKARFREKQNL
FTIPIGCYDCHDAQTMKLRITRPALKDALKRIGRDPEKFTEKELKNYICAQCHVTYYIDKATNRVIFPWDGGVDPEDMYR
HYEKRNFYEWVHPLSGTKMLKSRHPEFEMFSGSVHSAVGLTCVDCHMPYVYEGGKKYSSHWWTSPLRTLEQSCRKCHREE
EAKLRERVFYIQDRTYDLLNRAGKANEEAIKAINEAVYSPGADKALIEKARKLHREAQWYWDYVSSENSMGFHNPEKALT
TLGKSIDLALRAKATALQAKK
>Mature_420_residues
TRGITIGIIIVALTLLAGTFVAYITAKNDVPYRTLTTGFKKDEIKPEKYGQKFPVEYKYYLKNKEMALAPSKYGGSVKRD
HLKEFPYMKTLWAGFAFSKDYTEDRGHVYALEDVKETKRISEKTALACMTCKSSQVPVYMDKWGVDNFYKARFREKQNLF
TIPIGCYDCHDAQTMKLRITRPALKDALKRIGRDPEKFTEKELKNYICAQCHVTYYIDKATNRVIFPWDGGVDPEDMYRH
YEKRNFYEWVHPLSGTKMLKSRHPEFEMFSGSVHSAVGLTCVDCHMPYVYEGGKKYSSHWWTSPLRTLEQSCRKCHREEE
AKLRERVFYIQDRTYDLLNRAGKANEEAIKAINEAVYSPGADKALIEKARKLHREAQWYWDYVSSENSMGFHNPEKALTT
LGKSIDLALRAKATALQAKK

Specific function: Plays a role in nitrite reduction [H]

COG id: COG3303

COG function: function code P; Formate-dependent nitrite reductase, periplasmic cytochrome c552 subunit

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the cytochrome c-552 family [H]

Homologues:

Organism=Escherichia coli, GI1790506, Length=432, Percent_Identity=31.4814814814815, Blast_Score=220, Evalue=1e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003321
- InterPro:   IPR017570
- InterPro:   IPR011031 [H]

Pfam domain/function: PF02335 Cytochrom_C552 [H]

EC number: =1.7.2.2 [H]

Molecular weight: Translated: 48847; Mature: 48715

Theoretical pI: Translated: 9.47; Mature: 9.47

Prosite motif: PS51008 MULTIHEME_CYTC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRGITIGIIIVALTLLAGTFVAYITAKNDVPYRTLTTGFKKDEIKPEKYGQKFPVEYKY
CCCCCHHHHHHHHHHHHHHHHHEEEEECCCCCCHHHHCCCCCCCCCHHHCCCCCCCEEEE
YLKNKEMALAPSKYGGSVKRDHLKEFPYMKTLWAGFAFSKDYTEDRGHVYALEDVKETKR
EECCCCEEECCCCCCCCCCHHHHHHCCHHHHHHHHHHCCCCCCCCCCCEEEHHHHHHHHH
ISEKTALACMTCKSSQVPVYMDKWGVDNFYKARFREKQNLFTIPIGCYDCHDAQTMKLRI
HHHHHHHHEEECCCCCCCEEEECCCCCHHHHHHHHHCCCEEEEEECCEECCCCCEEEEEE
TRPALKDALKRIGRDPEKFTEKELKNYICAQCHVTYYIDKATNRVIFPWDGGVDPEDMYR
ECHHHHHHHHHHCCCHHHHHHHHHHHHHHHHEEEEEEEECCCCEEEECCCCCCCHHHHHH
HYEKRNFYEWVHPLSGTKMLKSRHPEFEMFSGSVHSAVGLTCVDCHMPYVYEGGKKYSSH
HHHHCCHHHHHCCCCCHHHHHHCCCCCEEECCCHHHHHCCEEEECCCCEEECCCCCCCCC
WWTSPLRTLEQSCRKCHREEEAKLRERVFYIQDRTYDLLNRAGKANEEAIKAINEAVYSP
CCCHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCHHHHHHHCCCCCHHHHHHHHHHHCCC
GADKALIEKARKLHREAQWYWDYVSSENSMGFHNPEKALTTLGKSIDLALRAKATALQAK
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHCCC
K
C
>Mature Secondary Structure 
TRGITIGIIIVALTLLAGTFVAYITAKNDVPYRTLTTGFKKDEIKPEKYGQKFPVEYKY
CCCCHHHHHHHHHHHHHHHHHEEEEECCCCCCHHHHCCCCCCCCCHHHCCCCCCCEEEE
YLKNKEMALAPSKYGGSVKRDHLKEFPYMKTLWAGFAFSKDYTEDRGHVYALEDVKETKR
EECCCCEEECCCCCCCCCCHHHHHHCCHHHHHHHHHHCCCCCCCCCCCEEEHHHHHHHHH
ISEKTALACMTCKSSQVPVYMDKWGVDNFYKARFREKQNLFTIPIGCYDCHDAQTMKLRI
HHHHHHHHEEECCCCCCCEEEECCCCCHHHHHHHHHCCCEEEEEECCEECCCCCEEEEEE
TRPALKDALKRIGRDPEKFTEKELKNYICAQCHVTYYIDKATNRVIFPWDGGVDPEDMYR
ECHHHHHHHHHHCCCHHHHHHHHHHHHHHHHEEEEEEEECCCCEEEECCCCCCCHHHHHH
HYEKRNFYEWVHPLSGTKMLKSRHPEFEMFSGSVHSAVGLTCVDCHMPYVYEGGKKYSSH
HHHHCCHHHHHCCCCCHHHHHHCCCCCEEECCCHHHHHCCEEEECCCCEEECCCCCCCCC
WWTSPLRTLEQSCRKCHREEEAKLRERVFYIQDRTYDLLNRAGKANEEAIKAINEAVYSP
CCCHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCHHHHHHHCCCCCHHHHHHHHHHHCCC
GADKALIEKARKLHREAQWYWDYVSSENSMGFHNPEKALTTLGKSIDLALRAKATALQAK
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHCCC
K
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA