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The map label for this gene is eno

Identifier: 78042740

GI number: 78042740

Start: 250744

End: 252030

Strand: Direct

Name: eno

Synonym: CHY_0284

Alternate gene names: 78042740

Gene position: 250744-252030 (Clockwise)

Preceding gene: 78042968

Following gene: 78044803

Centisome position: 10.44

GC content: 47.4

Gene sequence:

>1287_bases
ATGAGCGAAATTCGGGAAGTTATCGCCCGGGAAATTTTAGACTCCCGGGGAAATCCTACTGTTGAGGTAGATGTGATCCT
GGAAGATGGTACCTGGGGAAGAGCTGCGGTGCCATCCGGTGCCTCTACCGGGGCGTTTGAAGCGGTGGAACTCCGGGATA
ATGACCCCCAAAGATATCTGGGTAAGGGGGTTACCCAGGCGGTAGAAAATGTCAACTCCATTATTGGACCGGAAATTCTC
GGGTTTGATGCCTTGGACCAGGTGGGGATTGACGAGTACCTCATTGAATTAGACGGTACTCCCAATAAAGGTAAGCTTGG
TGCCAACGCTATCCTTGGAGTATCGATGGCGGTGGCTAAAGCGGCGGCAAATTACCTGGGGCTTCCCCTGTACCGTTATC
TTGGGGGAACCAATGCCCGGGTACTGCCGGCGCCGATGATGAATATTTTAAACGGTGGCAAGCATGCCGATAACAACGTG
GACATTCAGGAATTCATGATCATGCCCCTTGGTGCTTCTTCCTTCAGTGAAGCATTACGGATGGGTGCGGAAGTGTTTCA
CAACCTCAAAAAAGTTTTAAAGGGGAAAGGATATAACACCGCTGTAGGTGATGAAGGAGGATTTGCTCCAAACCTCAAGT
CCAACGAAGAAGCCATTGAAGTAATCGTTGAGGCTATCGAAAAAGCAGGCTACACTCCGGGAAAAGACATCTATATCGCC
CTTGATGTGGCGGCAACAGAATTGTACAAGGACGGGTTTTATGTCCTGGAAGGAGAAGGGGTTAAAAAATCTGCTGCGGA
AATGGTGGAGTATTACGAAAAACTTTGTGCTAAGTATCCGATAATTAGCATTGAAGATGGAATGAGCGAAGAAGACTGGG
ATGGCTGGAAGCTTTTAACCGAGCGCCTGGGGAAGAAAATTCAGCTGGTAGGGGACGATTTGTTTGTTACCAACGTTGAG
CGCCTTCAGAAAGGGATTGATTTGGGAGTTGCCAACAGTATTTTAATTAAGTTAAATCAAATCGGTACCATTACCGAAAC
TTTAAATACCATCGAGCTTGCCCGGCAAAACGGTTATACTACCATTATTTCCCACCGTTCGGGAGAAACCGAGGATACTA
CCCTGGCGGATTTAGCGGTGGCGGTAAATGCCGGTATGATTAAGACCGGAGCGCCGTCGCGGACCGACCGGGTAGCAAAA
TACAACCAGCTGTTAAGAATTGAGGAAGAATTGGACTATACTGCTATTTACAAAGGACTAAAAGCTTTTTATAATATAAA
AGCGTAA

Upstream 100 bases:

>100_bases
TAATGTGGCCCCGACGATTTTAAAAATTTTAGAGCTGGAAATACCCGAGGAAATGACCAGTGAGCCTTTATTTTAAATTT
TATGAAGGAGGGTTATTTTG

Downstream 100 bases:

>100_bases
TTTTTTAAAAGGCGGTCGGGAAGCAGCCTTCCCGGCCTTAAACTATTTTTTAACTTTAGGGGTTATGTATAAATATTTAA
AATAGTGCGAAAATTATTGT

Product: phosphopyruvate hydratase

Products: NA

Alternate protein names: 2-phospho-D-glycerate hydro-lyase; 2-phosphoglycerate dehydratase

Number of amino acids: Translated: 428; Mature: 427

Protein sequence:

>428_residues
MSEIREVIAREILDSRGNPTVEVDVILEDGTWGRAAVPSGASTGAFEAVELRDNDPQRYLGKGVTQAVENVNSIIGPEIL
GFDALDQVGIDEYLIELDGTPNKGKLGANAILGVSMAVAKAAANYLGLPLYRYLGGTNARVLPAPMMNILNGGKHADNNV
DIQEFMIMPLGASSFSEALRMGAEVFHNLKKVLKGKGYNTAVGDEGGFAPNLKSNEEAIEVIVEAIEKAGYTPGKDIYIA
LDVAATELYKDGFYVLEGEGVKKSAAEMVEYYEKLCAKYPIISIEDGMSEEDWDGWKLLTERLGKKIQLVGDDLFVTNVE
RLQKGIDLGVANSILIKLNQIGTITETLNTIELARQNGYTTIISHRSGETEDTTLADLAVAVNAGMIKTGAPSRTDRVAK
YNQLLRIEEELDYTAIYKGLKAFYNIKA

Sequences:

>Translated_428_residues
MSEIREVIAREILDSRGNPTVEVDVILEDGTWGRAAVPSGASTGAFEAVELRDNDPQRYLGKGVTQAVENVNSIIGPEIL
GFDALDQVGIDEYLIELDGTPNKGKLGANAILGVSMAVAKAAANYLGLPLYRYLGGTNARVLPAPMMNILNGGKHADNNV
DIQEFMIMPLGASSFSEALRMGAEVFHNLKKVLKGKGYNTAVGDEGGFAPNLKSNEEAIEVIVEAIEKAGYTPGKDIYIA
LDVAATELYKDGFYVLEGEGVKKSAAEMVEYYEKLCAKYPIISIEDGMSEEDWDGWKLLTERLGKKIQLVGDDLFVTNVE
RLQKGIDLGVANSILIKLNQIGTITETLNTIELARQNGYTTIISHRSGETEDTTLADLAVAVNAGMIKTGAPSRTDRVAK
YNQLLRIEEELDYTAIYKGLKAFYNIKA
>Mature_427_residues
SEIREVIAREILDSRGNPTVEVDVILEDGTWGRAAVPSGASTGAFEAVELRDNDPQRYLGKGVTQAVENVNSIIGPEILG
FDALDQVGIDEYLIELDGTPNKGKLGANAILGVSMAVAKAAANYLGLPLYRYLGGTNARVLPAPMMNILNGGKHADNNVD
IQEFMIMPLGASSFSEALRMGAEVFHNLKKVLKGKGYNTAVGDEGGFAPNLKSNEEAIEVIVEAIEKAGYTPGKDIYIAL
DVAATELYKDGFYVLEGEGVKKSAAEMVEYYEKLCAKYPIISIEDGMSEEDWDGWKLLTERLGKKIQLVGDDLFVTNVER
LQKGIDLGVANSILIKLNQIGTITETLNTIELARQNGYTTIISHRSGETEDTTLADLAVAVNAGMIKTGAPSRTDRVAKY
NQLLRIEEELDYTAIYKGLKAFYNIKA

Specific function: Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis

COG id: COG0148

COG function: function code G; Enolase

Gene ontology:

Cell location: Cytoplasm. Secreted. Cell surface. Note=Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the bacterial ce

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enolase family

Homologues:

Organism=Homo sapiens, GI301897477, Length=437, Percent_Identity=52.4027459954233, Blast_Score=434, Evalue=1e-122,
Organism=Homo sapiens, GI301897469, Length=437, Percent_Identity=52.4027459954233, Blast_Score=434, Evalue=1e-122,
Organism=Homo sapiens, GI5803011, Length=432, Percent_Identity=52.3148148148148, Blast_Score=431, Evalue=1e-121,
Organism=Homo sapiens, GI4503571, Length=434, Percent_Identity=51.3824884792627, Blast_Score=428, Evalue=1e-120,
Organism=Homo sapiens, GI301897479, Length=435, Percent_Identity=47.816091954023, Blast_Score=373, Evalue=1e-103,
Organism=Homo sapiens, GI169201331, Length=336, Percent_Identity=28.2738095238095, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI169201757, Length=336, Percent_Identity=28.2738095238095, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI239744207, Length=336, Percent_Identity=28.2738095238095, Blast_Score=114, Evalue=2e-25,
Organism=Escherichia coli, GI1789141, Length=426, Percent_Identity=63.1455399061033, Blast_Score=518, Evalue=1e-148,
Organism=Caenorhabditis elegans, GI71995829, Length=430, Percent_Identity=54.1860465116279, Blast_Score=445, Evalue=1e-125,
Organism=Caenorhabditis elegans, GI17536383, Length=430, Percent_Identity=54.1860465116279, Blast_Score=445, Evalue=1e-125,
Organism=Caenorhabditis elegans, GI32563855, Length=189, Percent_Identity=48.6772486772487, Blast_Score=187, Evalue=9e-48,
Organism=Saccharomyces cerevisiae, GI6323985, Length=434, Percent_Identity=49.5391705069124, Blast_Score=394, Evalue=1e-110,
Organism=Saccharomyces cerevisiae, GI6324974, Length=433, Percent_Identity=49.6535796766744, Blast_Score=394, Evalue=1e-110,
Organism=Saccharomyces cerevisiae, GI6324969, Length=433, Percent_Identity=49.6535796766744, Blast_Score=394, Evalue=1e-110,
Organism=Saccharomyces cerevisiae, GI6321693, Length=431, Percent_Identity=48.491879350348, Blast_Score=392, Evalue=1e-110,
Organism=Saccharomyces cerevisiae, GI6321968, Length=431, Percent_Identity=48.2598607888631, Blast_Score=369, Evalue=1e-103,
Organism=Drosophila melanogaster, GI24580918, Length=433, Percent_Identity=52.4249422632794, Blast_Score=417, Evalue=1e-117,
Organism=Drosophila melanogaster, GI24580916, Length=433, Percent_Identity=52.4249422632794, Blast_Score=417, Evalue=1e-117,
Organism=Drosophila melanogaster, GI24580920, Length=433, Percent_Identity=52.4249422632794, Blast_Score=417, Evalue=1e-117,
Organism=Drosophila melanogaster, GI24580914, Length=433, Percent_Identity=52.4249422632794, Blast_Score=417, Evalue=1e-117,
Organism=Drosophila melanogaster, GI281360527, Length=433, Percent_Identity=52.4249422632794, Blast_Score=416, Evalue=1e-116,
Organism=Drosophila melanogaster, GI17137654, Length=433, Percent_Identity=52.4249422632794, Blast_Score=416, Evalue=1e-116,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): ENO_CARHZ (Q3AFC8)

Other databases:

- EMBL:   CP000141
- RefSeq:   YP_359156.1
- HSSP:   P0A6P9
- ProteinModelPortal:   Q3AFC8
- SMR:   Q3AFC8
- STRING:   Q3AFC8
- GeneID:   3726912
- GenomeReviews:   CP000141_GR
- KEGG:   chy:CHY_0284
- NMPDR:   fig|246194.3.peg.295
- TIGR:   CHY_0284
- eggNOG:   COG0148
- HOGENOM:   HBG726599
- OMA:   DIAVGTN
- ProtClustDB:   PRK00077
- BioCyc:   CHYD246194:CHY_0284-MONOMER
- GO:   GO:0006096
- HAMAP:   MF_00318
- InterPro:   IPR000941
- InterPro:   IPR020810
- InterPro:   IPR020809
- InterPro:   IPR020811
- PIRSF:   PIRSF001400
- PRINTS:   PR00148
- TIGRFAMs:   TIGR01060

Pfam domain/function: PF00113 Enolase_C; PF03952 Enolase_N

EC number: =4.2.1.11

Molecular weight: Translated: 46452; Mature: 46321

Theoretical pI: Translated: 4.46; Mature: 4.46

Prosite motif: PS00164 ENOLASE

Important sites: ACT_SITE 205-205 ACT_SITE 337-337 BINDING 155-155 BINDING 164-164 BINDING 285-285 BINDING 312-312 BINDING 337-337 BINDING 388-388

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEIREVIAREILDSRGNPTVEVDVILEDGTWGRAAVPSGASTGAFEAVELRDNDPQRYL
CHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCEEEEEECCCCHHHHH
GKGVTQAVENVNSIIGPEILGFDALDQVGIDEYLIELDGTPNKGKLGANAILGVSMAVAK
HHHHHHHHHHHHHHHCCHHCCCCHHHHCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHH
AAANYLGLPLYRYLGGTNARVLPAPMMNILNGGKHADNNVDIQEFMIMPLGASSFSEALR
HHHHHHCCHHHHHHCCCCCEECCHHHHHHHCCCCCCCCCCCHHHHEEECCCCHHHHHHHH
MGAEVFHNLKKVLKGKGYNTAVGDEGGFAPNLKSNEEAIEVIVEAIEKAGYTPGKDIYIA
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEE
LDVAATELYKDGFYVLEGEGVKKSAAEMVEYYEKLCAKYPIISIEDGMSEEDWDGWKLLT
EEHHHHHHHHCCEEEEECCCCHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCHHHHHH
ERLGKKIQLVGDDLFVTNVERLQKGIDLGVANSILIKLNQIGTITETLNTIELARQNGYT
HHCCCEEEEECCCHHHHHHHHHHHCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCE
TIISHRSGETEDTTLADLAVAVNAGMIKTGAPSRTDRVAKYNQLLRIEEELDYTAIYKGL
EEEECCCCCCCCHHHHHHHHHHCCCEEECCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHH
KAFYNIKA
HHHHCCCC
>Mature Secondary Structure 
SEIREVIAREILDSRGNPTVEVDVILEDGTWGRAAVPSGASTGAFEAVELRDNDPQRYL
HHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCEEEEEECCCCHHHHH
GKGVTQAVENVNSIIGPEILGFDALDQVGIDEYLIELDGTPNKGKLGANAILGVSMAVAK
HHHHHHHHHHHHHHHCCHHCCCCHHHHCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHH
AAANYLGLPLYRYLGGTNARVLPAPMMNILNGGKHADNNVDIQEFMIMPLGASSFSEALR
HHHHHHCCHHHHHHCCCCCEECCHHHHHHHCCCCCCCCCCCHHHHEEECCCCHHHHHHHH
MGAEVFHNLKKVLKGKGYNTAVGDEGGFAPNLKSNEEAIEVIVEAIEKAGYTPGKDIYIA
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEE
LDVAATELYKDGFYVLEGEGVKKSAAEMVEYYEKLCAKYPIISIEDGMSEEDWDGWKLLT
EEHHHHHHHHCCEEEEECCCCHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCHHHHHH
ERLGKKIQLVGDDLFVTNVERLQKGIDLGVANSILIKLNQIGTITETLNTIELARQNGYT
HHCCCEEEEECCCHHHHHHHHHHHCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCE
TIISHRSGETEDTTLADLAVAVNAGMIKTGAPSRTDRVAKYNQLLRIEEELDYTAIYKGL
EEEECCCCCCCCHHHHHHHHHHCCCEEECCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHH
KAFYNIKA
HHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA