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The map label for this gene is rph

Identifier: 78042648

GI number: 78042648

Start: 276278

End: 277042

Strand: Direct

Name: rph

Synonym: CHY_0314

Alternate gene names: 78042648

Gene position: 276278-277042 (Clockwise)

Preceding gene: 78044376

Following gene: 78043192

Centisome position: 11.5

GC content: 47.32

Gene sequence:

>765_bases
ATGCGAATTGATGGTAGGGAAAAAGACCAGTTACGAACGGTTAAAATAACCACAAACTACATAAAACATGCTGAAGGTTC
GGTTTTAATTGAAATGGGTGATACCCGGGTAGTTTGTACCGCTACCGTAGAAGATAAGGTGCCACCCTTTGCCAAGGGCG
AAGGCAAAGGGTGGATTACTGCGGAATACGGAATGCTTCCCCGGAGTACCGAAACCCGCAATGTTCGGGAAGCGACCAAA
GGTCGGCAATCGGGCCGGACCCTGGAAATTCAACGGCTAATTGGTCGAGCTTTAAGGGGAGTGGTTGATCTAAAAGCTCT
GGGGGAGCGAACTTTGTGGATTGATTGTGACGTAATTCAGGCGGATGGGGGTACCAGGACTGCAGCGATTACCGGCTCTT
TTGTGGCTTTAGCATTAGCGTTAAATAAGCTCGTTGAGGAGGGAATTTTACCGGTAATTCCCTTAAAGGATTTTGTGGCG
GCGGTAAGTGTGGGTATTGTTGACGGCGAGGAAATTTTGGACTTAAATTTTGAGGAAGATTCGAAGGCTTTAGTGGATAT
GAACGTGGTGATGACCGGTTCTAACCGTTTTGTGGAAGTGCAGGGTACCGGTGAAGAAGCAACTTTTTCAATGGAAGAGC
TGCAAAGGCTTTTAACACTGGCCCAGAAGGGGATCAGAGAGTTAATTGAACTTCAAAAACAGGCCCTGGGAGATATTGCC
CGGAGGATAGGGATGGAAAATGCAGCGGATAGTAATAGCCAGTAA

Upstream 100 bases:

>100_bases
GTTTTAAAATACTTAAAGAATTAAAATACGATCCTATTGAAAAACTTTATATAATTGCCGGGGTTGTTGGTCAGCAGGAC
AACGAAAATGAGGTGATTTA

Downstream 100 bases:

>100_bases
AAATCAAGGAAAGATCAAAGAATTTTCAGCGCTTTTAAGTTCTCTGGGTTTTTCCTTTGTTAGTCTTTTGGATTTTCCGG
AAATCGGGGAAATACCCGAA

Product: ribonuclease PH

Products: NA

Alternate protein names: RNase PH; tRNA nucleotidyltransferase

Number of amino acids: Translated: 254; Mature: 254

Protein sequence:

>254_residues
MRIDGREKDQLRTVKITTNYIKHAEGSVLIEMGDTRVVCTATVEDKVPPFAKGEGKGWITAEYGMLPRSTETRNVREATK
GRQSGRTLEIQRLIGRALRGVVDLKALGERTLWIDCDVIQADGGTRTAAITGSFVALALALNKLVEEGILPVIPLKDFVA
AVSVGIVDGEEILDLNFEEDSKALVDMNVVMTGSNRFVEVQGTGEEATFSMEELQRLLTLAQKGIRELIELQKQALGDIA
RRIGMENAADSNSQ

Sequences:

>Translated_254_residues
MRIDGREKDQLRTVKITTNYIKHAEGSVLIEMGDTRVVCTATVEDKVPPFAKGEGKGWITAEYGMLPRSTETRNVREATK
GRQSGRTLEIQRLIGRALRGVVDLKALGERTLWIDCDVIQADGGTRTAAITGSFVALALALNKLVEEGILPVIPLKDFVA
AVSVGIVDGEEILDLNFEEDSKALVDMNVVMTGSNRFVEVQGTGEEATFSMEELQRLLTLAQKGIRELIELQKQALGDIA
RRIGMENAADSNSQ
>Mature_254_residues
MRIDGREKDQLRTVKITTNYIKHAEGSVLIEMGDTRVVCTATVEDKVPPFAKGEGKGWITAEYGMLPRSTETRNVREATK
GRQSGRTLEIQRLIGRALRGVVDLKALGERTLWIDCDVIQADGGTRTAAITGSFVALALALNKLVEEGILPVIPLKDFVA
AVSVGIVDGEEILDLNFEEDSKALVDMNVVMTGSNRFVEVQGTGEEATFSMEELQRLLTLAQKGIRELIELQKQALGDIA
RRIGMENAADSNSQ

Specific function: Phosphorolytic exoribonuclease that removes nucleotide residues following the -CCA terminus of tRNA and adds nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates

COG id: COG0689

COG function: function code J; RNase PH

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase PH family

Homologues:

Organism=Escherichia coli, GI157672248, Length=210, Percent_Identity=66.1904761904762, Blast_Score=283, Evalue=1e-77,
Organism=Caenorhabditis elegans, GI71981632, Length=189, Percent_Identity=31.2169312169312, Blast_Score=83, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNPH_CARHZ (Q3AFA0)

Other databases:

- EMBL:   CP000141
- RefSeq:   YP_359184.1
- HSSP:   O29756
- ProteinModelPortal:   Q3AFA0
- SMR:   Q3AFA0
- STRING:   Q3AFA0
- GeneID:   3726312
- GenomeReviews:   CP000141_GR
- KEGG:   chy:CHY_0314
- NMPDR:   fig|246194.3.peg.402
- TIGR:   CHY_0314
- eggNOG:   COG0689
- HOGENOM:   HBG737187
- OMA:   MLPRATG
- ProtClustDB:   PRK00173
- BioCyc:   CHYD246194:CHY_0314-MONOMER
- HAMAP:   MF_00564
- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR020568
- InterPro:   IPR002381
- InterPro:   IPR018336
- TIGRFAMs:   TIGR01966

Pfam domain/function: PF01138 RNase_PH; PF03725 RNase_PH_C; SSF55666 3_ExoRNase; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.7.56

Molecular weight: Translated: 27772; Mature: 27772

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: PS01277 RIBONUCLEASE_PH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIDGREKDQLRTVKITTNYIKHAEGSVLIEMGDTRVVCTATVEDKVPPFAKGEGKGWIT
CCCCCCCCCCEEEEEEEHHHHHCCCCCEEEEECCCEEEEEEEECCCCCCCCCCCCCEEEE
AEYGMLPRSTETRNVREATKGRQSGRTLEIQRLIGRALRGVVDLKALGERTLWIDCDVIQ
EECCCCCCCCCCCHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEEE
ADGGTRTAAITGSFVALALALNKLVEEGILPVIPLKDFVAAVSVGIVDGEEILDLNFEED
CCCCCEEEEEHHHHHHHHHHHHHHHHHCCCCEECHHHHHHHHHHEEECCHHEEECCCCCC
SKALVDMNVVMTGSNRFVEVQGTGEEATFSMEELQRLLTLAQKGIRELIELQKQALGDIA
CCEEEEEEEEEECCCEEEEEEECCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RRIGMENAADSNSQ
HHHCCCCCCCCCCC
>Mature Secondary Structure
MRIDGREKDQLRTVKITTNYIKHAEGSVLIEMGDTRVVCTATVEDKVPPFAKGEGKGWIT
CCCCCCCCCCEEEEEEEHHHHHCCCCCEEEEECCCEEEEEEEECCCCCCCCCCCCCEEEE
AEYGMLPRSTETRNVREATKGRQSGRTLEIQRLIGRALRGVVDLKALGERTLWIDCDVIQ
EECCCCCCCCCCCHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEEE
ADGGTRTAAITGSFVALALALNKLVEEGILPVIPLKDFVAAVSVGIVDGEEILDLNFEED
CCCCCEEEEEHHHHHHHHHHHHHHHHHCCCCEECHHHHHHHHHHEEECCHHEEECCCCCC
SKALVDMNVVMTGSNRFVEVQGTGEEATFSMEELQRLLTLAQKGIRELIELQKQALGDIA
CCEEEEEEEEEECCCEEEEEEECCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RRIGMENAADSNSQ
HHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA