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The map label for this gene is dadA [C]

Identifier: 77761162

GI number: 77761162

Start: 2038231

End: 2039460

Strand: Direct

Name: dadA [C]

Synonym: XC_1698

Alternate gene names: 77761162

Gene position: 2038231-2039460 (Clockwise)

Preceding gene: 66768021

Following gene: 66768023

Centisome position: 39.59

GC content: 72.03

Gene sequence:

>1230_bases
ATGCAGCCGCCACCGCGCGCGCAGGGCGGTACCGGTGCAGCGCCTGCTGCGGCGACTGCCGTGCCCGAAGCCGCACCGCC
ATCACATAGCGACACGCACGCCACCGCCGCTTCAACGCGCCGCAGCTACGACCTGATTGTGGTCGGCGCCGGCATCGTTG
GCGCGGCCTGCGCGGAGGCCGCCGCCGCCGAGGGCTTGCGCGTGGCGATCGTGGAACCGGGGCCGATCGGCGGTGGCTCC
ACCGCCGCGGCAATGGGGCACCTGGTGGCGATGGATGACGACCCCGCCGAGCTGGCCTTATCGGCGTATTCGCTGCGCCT
GTGGGAGCGGTTTGCCGAGTTGAGCGAGGCCGAATTCAGCCGGTGCGGCACGCTGTGGGTGGCCCGCGATGCGCGCGAAC
TGGCAGCGGTGCCGGCCAAGATTGCGCGGCTGGCCGCCGCCGGCCTGCGTGCCGAAGCGATCGACGCCACGCAGCTCTAC
GCGCTGGAACCACACCTGGTGCCCGGCCTGGCGGGTGGCATGCGCGTGCCTGACGAGGCAGTGGTGTATCCGCCGCGGGT
GGCGCGGCATCTGGTGAGCCTGGCTTGCACCGCAGGCGCGCACTTGTTCGCCGGCCGCAAGGTCGAGCAGCTGCAGGTCG
GCGGCGTGCGTCTGGATGATGGGCAGGCGCTGGCCGGGCCGGTGCTGGTTGCCAGTGGCGTGGCCTTGCCCGCCTTGTTG
CCCGAACTGGCGCTGCGCCCGCGCAAGGGCCATCTGGTGATCACCGACCGCCATCCGGGGCTGATCCGGCATCAGTTGCT
GGAACTGGGCTATGCAGATTCCGCACATGGCGCCGACGACAGCAGCGTGGCCTTCAATGTGCAACCGCGGCCGACCGGGC
AGATCCTGATCGGCTCCTCGCGCCAATACGGCGCGGACGACCGCACGCTGTCGATGCCGATCCTGCAGCAGATGCTGCAA
CGCGCCTTCGCGTATTTGCCGGTACTCCGCGAGCTGCAGGCGATCCGGGTATGGACCGGCCTGCGCCCGGCCACCCCGGA
TGGCCGGCCGTATCTGGGGCCGGTGCCGGGCCGGCGCGATGTGTGGGTGGCGGCCGGCCACGAGGGCTTGGGGGTTACCA
CGGCGCTGGGGAGCGCACGGGTGATCGTGGACAGCCTGCTCGGGCGCACGCCGGCGATCGACCCGGCGCCCTATGCGCCC
GCCCGCGCGGTACAGGGCGTGGCGGCATGA

Upstream 100 bases:

>100_bases
GTGGCATTGCGCCGCGCATCAGCGGGCACGCGTTCATCACCGCGCGGTCGCAACTGCTGATCGATCCGGCCGACCCATTC
GCCTGGGGCATCGTGGCCTG

Downstream 100 bases:

>100_bases
GCGGCATGGTGCGCCTACATGTGGATGCGCAGCCGGTGGAGGTGCCGCTGGGCGTCAGTGTTGCGGCCGCCGTGGCGCAG
GCAACGCTGCAGTTTCGCCA

Product: D-amino acid oxidase

Products: FADH2; ammonia; Pyruvate [C]

Alternate protein names: NA

Number of amino acids: Translated: 409; Mature: 409

Protein sequence:

>409_residues
MQPPPRAQGGTGAAPAAATAVPEAAPPSHSDTHATAASTRRSYDLIVVGAGIVGAACAEAAAAEGLRVAIVEPGPIGGGS
TAAAMGHLVAMDDDPAELALSAYSLRLWERFAELSEAEFSRCGTLWVARDARELAAVPAKIARLAAAGLRAEAIDATQLY
ALEPHLVPGLAGGMRVPDEAVVYPPRVARHLVSLACTAGAHLFAGRKVEQLQVGGVRLDDGQALAGPVLVASGVALPALL
PELALRPRKGHLVITDRHPGLIRHQLLELGYADSAHGADDSSVAFNVQPRPTGQILIGSSRQYGADDRTLSMPILQQMLQ
RAFAYLPVLRELQAIRVWTGLRPATPDGRPYLGPVPGRRDVWVAAGHEGLGVTTALGSARVIVDSLLGRTPAIDPAPYAP
ARAVQGVAA

Sequences:

>Translated_409_residues
MQPPPRAQGGTGAAPAAATAVPEAAPPSHSDTHATAASTRRSYDLIVVGAGIVGAACAEAAAAEGLRVAIVEPGPIGGGS
TAAAMGHLVAMDDDPAELALSAYSLRLWERFAELSEAEFSRCGTLWVARDARELAAVPAKIARLAAAGLRAEAIDATQLY
ALEPHLVPGLAGGMRVPDEAVVYPPRVARHLVSLACTAGAHLFAGRKVEQLQVGGVRLDDGQALAGPVLVASGVALPALL
PELALRPRKGHLVITDRHPGLIRHQLLELGYADSAHGADDSSVAFNVQPRPTGQILIGSSRQYGADDRTLSMPILQQMLQ
RAFAYLPVLRELQAIRVWTGLRPATPDGRPYLGPVPGRRDVWVAAGHEGLGVTTALGSARVIVDSLLGRTPAIDPAPYAP
ARAVQGVAA
>Mature_409_residues
MQPPPRAQGGTGAAPAAATAVPEAAPPSHSDTHATAASTRRSYDLIVVGAGIVGAACAEAAAAEGLRVAIVEPGPIGGGS
TAAAMGHLVAMDDDPAELALSAYSLRLWERFAELSEAEFSRCGTLWVARDARELAAVPAKIARLAAAGLRAEAIDATQLY
ALEPHLVPGLAGGMRVPDEAVVYPPRVARHLVSLACTAGAHLFAGRKVEQLQVGGVRLDDGQALAGPVLVASGVALPALL
PELALRPRKGHLVITDRHPGLIRHQLLELGYADSAHGADDSSVAFNVQPRPTGQILIGSSRQYGADDRTLSMPILQQMLQ
RAFAYLPVLRELQAIRVWTGLRPATPDGRPYLGPVPGRRDVWVAAGHEGLGVTTALGSARVIVDSLLGRTPAIDPAPYAP
ARAVQGVAA

Specific function: Oxidative Deamination Of D-Amino Acids. [C]

COG id: COG0665

COG function: function code E; Glycine/D-amino acid oxidases (deaminating)

Gene ontology:

Cell location: Inner Membrane-Bound [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DadA oxidoreductase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006076
- InterPro:   IPR012727 [H]

Pfam domain/function: PF01266 DAO [H]

EC number: 1.4.99.1 [C]

Molecular weight: Translated: 42350; Mature: 42350

Theoretical pI: Translated: 7.12; Mature: 7.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQPPPRAQGGTGAAPAAATAVPEAAPPSHSDTHATAASTRRSYDLIVVGAGIVGAACAEA
CCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCHHHHHHCCCCEEEEEECCCHHHHHHHHH
AAAEGLRVAIVEPGPIGGGSTAAAMGHLVAMDDDPAELALSAYSLRLWERFAELSEAEFS
HHHCCCEEEEECCCCCCCCCHHHHHCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHH
RCGTLWVARDARELAAVPAKIARLAAAGLRAEAIDATQLYALEPHLVPGLAGGMRVPDEA
HCCEEEEECCHHHHHHHHHHHHHHHHHCCCHHHCCCEEEEEECCCCCCCCCCCCCCCCCC
VVYPPRVARHLVSLACTAGAHLFAGRKVEQLQVGGVRLDDGQALAGPVLVASGVALPALL
EECCHHHHHHHHHHHHHCCCHHHCCCCEEEEEECCEEECCCCCCCCHHHHHCCCHHHHHH
PELALRPRKGHLVITDRHPGLIRHQLLELGYADSAHGADDSSVAFNVQPRPTGQILIGSS
HHHHCCCCCCCEEEECCCCCHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCEEEEECC
RQYGADDRTLSMPILQQMLQRAFAYLPVLRELQAIRVWTGLRPATPDGRPYLGPVPGRRD
CCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
VWVAAGHEGLGVTTALGSARVIVDSLLGRTPAIDPAPYAPARAVQGVAA
EEEEECCCCCCEEECCCCHHHHHHHHHCCCCCCCCCCCCHHHHHCCCCC
>Mature Secondary Structure
MQPPPRAQGGTGAAPAAATAVPEAAPPSHSDTHATAASTRRSYDLIVVGAGIVGAACAEA
CCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCHHHHHHCCCCEEEEEECCCHHHHHHHHH
AAAEGLRVAIVEPGPIGGGSTAAAMGHLVAMDDDPAELALSAYSLRLWERFAELSEAEFS
HHHCCCEEEEECCCCCCCCCHHHHHCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHH
RCGTLWVARDARELAAVPAKIARLAAAGLRAEAIDATQLYALEPHLVPGLAGGMRVPDEA
HCCEEEEECCHHHHHHHHHHHHHHHHHCCCHHHCCCEEEEEECCCCCCCCCCCCCCCCCC
VVYPPRVARHLVSLACTAGAHLFAGRKVEQLQVGGVRLDDGQALAGPVLVASGVALPALL
EECCHHHHHHHHHHHHHCCCHHHCCCCEEEEEECCEEECCCCCCCCHHHHHCCCHHHHHH
PELALRPRKGHLVITDRHPGLIRHQLLELGYADSAHGADDSSVAFNVQPRPTGQILIGSS
HHHHCCCCCCCEEEECCCCCHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCEEEEECC
RQYGADDRTLSMPILQQMLQRAFAYLPVLRELQAIRVWTGLRPATPDGRPYLGPVPGRRD
CCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
VWVAAGHEGLGVTTALGSARVIVDSLLGRTPAIDPAPYAPARAVQGVAA
EEEEECCCCCCEEECCCCHHHHHHHHHCCCCCCCCCCCCHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Iron [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 40 {D-proline}} 0.13 {K3Fe(CN)6}} 0.63 {dichloroindophenol}} 0.01 {dichloroindophenol}} 11 {D-methionine}} 6 {D-phenylalanine}} 12 {D-alpha-aminobutyrate}} 0.15 {cytochrome} 19 {D-serine}} 29 {D-alanine}} 6.4 {D-alanine}} 5.3 {D

Substrates: D-Alanine; FAD; H2O [C]

Specific reaction: D-Alanine + FAD + H2O --> FADH2 + ammonia + Pyruvate [C]

General reaction: Oxidative deamination; Redox reaction [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8682785; 10984043; 8097014 [H]