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The map label for this gene is proS

Identifier: 77748729

GI number: 77748729

Start: 4230585

End: 4232279

Strand: Direct

Name: proS

Synonym: XAC3567

Alternate gene names: 77748729

Gene position: 4230585-4232279 (Clockwise)

Preceding gene: 21244291

Following gene: 21244293

Centisome position: 81.74

GC content: 65.9

Gene sequence:

>1695_bases
ATGCGTCTTTCCCAGTTCCACCTGCACACCACCAAGGAAACCCCGGCCGACGCCGAGCTGGTCAGCCACCGCCTGATGCT
GCGCGCGGGCATGATCCGCAAGCTGGCCTCCGGGCTGTACACCTGGTCGCCGCTGGGCTTGCGCGTGTTGCGCAAGGTCG
AGGCCATCGTGCGCGAGGAGATGAACCGCGCCGGTGCAGTGGAAGTGCTGTTTCCCACCATCCAGCCACGCGAGCTGTGG
GACGCCACCGGGCGTTGGGAAAAGTTCGGCGGGCAGCTGCTCAAGATCAAGGACCGCAAGGAACAGGAGTTCTGCTACAG
CCCCACCGCCGAAGAGGCCGCTGCCGAATTCGCGCGCCAGGAGATCAACAGCTACAAGCAGCTGCCGCTGAATTTCTATC
AGATCCAGACCAAATTCCGCGATGAGATCCGCCCGCGCTTCGGCGTGATGCGTGCGCGCGAGTTCCTGATGAAGGACGCG
TATTCGTTCCACCTCACCGACGAGGACATGGCGCGCGAATACGACAACATGCGCGCCGCCTATACGCGCATCTTCACCCG
CCTGGGACTGGACTTCCGCGCCGTGCAGGCAGATTCCGGCGCCATCGGCGGCGATGCATCGCAGGAATTCCACGTCATCG
CCGACTCCGGCGAAGACTCGCTGGCGTTCTCCACTGGCTCCGATTACGCCGCCAATGTGGAAGCCGCCAGTGCGGCACCG
CCTGCCCCGCGCGCCGCGGCCAGCGAAGCGATGCAGCAGGTGGCAACGCCCACCCAGAAGACCTGTGAAGACGTGGCCCA
GTTGCTCGGCATCGCGCTGCAGCGCACGGTCAAGTCGGTGGCGGTGATGACGCAGGCCGGCTTCGTGCTGGTGCTGGTGC
GTGGCGATCACGCGGTCAACGAGATCAAGCTGGCCAAGGTGCCCGGACTGGCCGATTACCGCCTGGCCAACGAAAGCGAG
ATCCGCGAACACCTCGGCTGCGAGCCGGGCTTCCTGGGTCCGGTCAACACCGCGCGCCCGGTGCGCGTAGTGGCCGACCG
CGATGTCGCCGCACTGGCCGATTTTGTGGTCGGCGCGAATGTGTCCGGCGCCCACCTGGTCGGCGTCAATTGGGGCCGCG
ATCTGCCCGAACCGGAGACCGTGGCCGATGTGCGCAACGTGGTCGAAGGCGAACGCGCGGCCGATGGCGGCGAACTGCGC
CTGGCTCGCGGCATCGAGGTGGGCCATGTATTCCAGCTTGGCAGCCAGTACGCGCAGGCGCTGCAAGCCACCGTCATCGA
CGAAGGTGGCAAGGCGGCGGTGATGAAGATGGGCTGCTACGGCATCGGCATTTCGCGCATCGTGGCCGCGGCGATCGAAC
AGAACCATGACGACGCCGGCATCATCTGGCCCGCGCCGATGGCGCCGTGGCAGGTGGTGGTCTGCGTGATCAATCCCAAG
CAGGACCCGCAGGTGCTCGCCGCCGCCCAGGCCTTGCTGGACCAATTGCTTGCCGCCGGCCTGGATGCTGCCCTGGACGA
CCGCGGACTGCGCCCGGGCGCGATGTTCGCCGATATGGAATTGCTGGGCATTCCGCATCGGGTAGTGGTCTCGGAACGTG
GATTGGCGGCCGGCACTTTTGAATATCGCGCCCGTACCGCCGATGCAGCGGAAAGCCTGGATAAAGCCGGGTTATTCTCC
CGCCTGGGGCATTGA

Upstream 100 bases:

>100_bases
CGGTCCGCCCTGGCCAGCGACAATCCGCAACGCGCGCCCGGCGATGACCGGCAGGGCCCGAGACTGGCAAACTAGCGGCT
TTCCGCCCAAGCGAAGCCCG

Downstream 100 bases:

>100_bases
TCAAACCCCGGGCATCGGCTTCAAATGGATGAACACAAAGCGCCGAATTATTTCGGCGCATTTGTCATACCGGTTTTCTC
ACTTTATGATGGCCACCGCT

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS

Number of amino acids: Translated: 564; Mature: 564

Protein sequence:

>564_residues
MRLSQFHLHTTKETPADAELVSHRLMLRAGMIRKLASGLYTWSPLGLRVLRKVEAIVREEMNRAGAVEVLFPTIQPRELW
DATGRWEKFGGQLLKIKDRKEQEFCYSPTAEEAAAEFARQEINSYKQLPLNFYQIQTKFRDEIRPRFGVMRAREFLMKDA
YSFHLTDEDMAREYDNMRAAYTRIFTRLGLDFRAVQADSGAIGGDASQEFHVIADSGEDSLAFSTGSDYAANVEAASAAP
PAPRAAASEAMQQVATPTQKTCEDVAQLLGIALQRTVKSVAVMTQAGFVLVLVRGDHAVNEIKLAKVPGLADYRLANESE
IREHLGCEPGFLGPVNTARPVRVVADRDVAALADFVVGANVSGAHLVGVNWGRDLPEPETVADVRNVVEGERAADGGELR
LARGIEVGHVFQLGSQYAQALQATVIDEGGKAAVMKMGCYGIGISRIVAAAIEQNHDDAGIIWPAPMAPWQVVVCVINPK
QDPQVLAAAQALLDQLLAAGLDAALDDRGLRPGAMFADMELLGIPHRVVVSERGLAAGTFEYRARTADAAESLDKAGLFS
RLGH

Sequences:

>Translated_564_residues
MRLSQFHLHTTKETPADAELVSHRLMLRAGMIRKLASGLYTWSPLGLRVLRKVEAIVREEMNRAGAVEVLFPTIQPRELW
DATGRWEKFGGQLLKIKDRKEQEFCYSPTAEEAAAEFARQEINSYKQLPLNFYQIQTKFRDEIRPRFGVMRAREFLMKDA
YSFHLTDEDMAREYDNMRAAYTRIFTRLGLDFRAVQADSGAIGGDASQEFHVIADSGEDSLAFSTGSDYAANVEAASAAP
PAPRAAASEAMQQVATPTQKTCEDVAQLLGIALQRTVKSVAVMTQAGFVLVLVRGDHAVNEIKLAKVPGLADYRLANESE
IREHLGCEPGFLGPVNTARPVRVVADRDVAALADFVVGANVSGAHLVGVNWGRDLPEPETVADVRNVVEGERAADGGELR
LARGIEVGHVFQLGSQYAQALQATVIDEGGKAAVMKMGCYGIGISRIVAAAIEQNHDDAGIIWPAPMAPWQVVVCVINPK
QDPQVLAAAQALLDQLLAAGLDAALDDRGLRPGAMFADMELLGIPHRVVVSERGLAAGTFEYRARTADAAESLDKAGLFS
RLGH
>Mature_564_residues
MRLSQFHLHTTKETPADAELVSHRLMLRAGMIRKLASGLYTWSPLGLRVLRKVEAIVREEMNRAGAVEVLFPTIQPRELW
DATGRWEKFGGQLLKIKDRKEQEFCYSPTAEEAAAEFARQEINSYKQLPLNFYQIQTKFRDEIRPRFGVMRAREFLMKDA
YSFHLTDEDMAREYDNMRAAYTRIFTRLGLDFRAVQADSGAIGGDASQEFHVIADSGEDSLAFSTGSDYAANVEAASAAP
PAPRAAASEAMQQVATPTQKTCEDVAQLLGIALQRTVKSVAVMTQAGFVLVLVRGDHAVNEIKLAKVPGLADYRLANESE
IREHLGCEPGFLGPVNTARPVRVVADRDVAALADFVVGANVSGAHLVGVNWGRDLPEPETVADVRNVVEGERAADGGELR
LARGIEVGHVFQLGSQYAQALQATVIDEGGKAAVMKMGCYGIGISRIVAAAIEQNHDDAGIIWPAPMAPWQVVVCVINPK
QDPQVLAAAQALLDQLLAAGLDAALDDRGLRPGAMFADMELLGIPHRVVVSERGLAAGTFEYRARTADAAESLDKAGLFS
RLGH

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids su

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily

Homologues:

Organism=Homo sapiens, GI34303926, Length=220, Percent_Identity=44.0909090909091, Blast_Score=196, Evalue=4e-50,
Organism=Escherichia coli, GI1786392, Length=552, Percent_Identity=56.3405797101449, Blast_Score=636, Evalue=0.0,
Organism=Escherichia coli, GI1788013, Length=157, Percent_Identity=25.4777070063694, Blast_Score=66, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI115532348, Length=207, Percent_Identity=40.0966183574879, Blast_Score=164, Evalue=1e-40,
Organism=Caenorhabditis elegans, GI193203271, Length=85, Percent_Identity=32.9411764705882, Blast_Score=71, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI71984184, Length=167, Percent_Identity=26.3473053892216, Blast_Score=69, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI71984192, Length=167, Percent_Identity=26.3473053892216, Blast_Score=69, Evalue=6e-12,
Organism=Saccharomyces cerevisiae, GI6320931, Length=556, Percent_Identity=29.8561151079137, Blast_Score=247, Evalue=3e-66,
Organism=Drosophila melanogaster, GI24656200, Length=229, Percent_Identity=37.117903930131, Blast_Score=171, Evalue=9e-43,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): SYP_XANAC (Q8PGP9)

Other databases:

- EMBL:   AE008923
- RefSeq:   NP_643874.2
- ProteinModelPortal:   Q8PGP9
- SMR:   Q8PGP9
- GeneID:   1157638
- GenomeReviews:   AE008923_GR
- KEGG:   xac:XAC3567
- NMPDR:   fig|190486.1.peg.3518
- HOGENOM:   HBG403504
- OMA:   DFVLGPT
- ProtClustDB:   PRK09194
- BioCyc:   XAXO190486:XAC3567-MONOMER
- BRENDA:   6.1.1.15
- GO:   GO:0005737
- HAMAP:   MF_01569
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004500
- InterPro:   IPR007214
- Gene3D:   G3DSA:3.40.50.800
- Gene3D:   G3DSA:3.90.960.10
- PRINTS:   PR01046
- TIGRFAMs:   TIGR00409

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; PF04073 YbaK; SSF52954 Anticodon_bd; SSF55826 YbaK/aa-tRNA-synth-assoc-reg

EC number: =6.1.1.15

Molecular weight: Translated: 61401; Mature: 61401

Theoretical pI: Translated: 5.54; Mature: 5.54

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLSQFHLHTTKETPADAELVSHRLMLRAGMIRKLASGLYTWSPLGLRVLRKVEAIVREE
CCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHH
MNRAGAVEVLFPTIQPRELWDATGRWEKFGGQLLKIKDRKEQEFCYSPTAEEAAAEFARQ
HHHCCCEEEEECCCCCHHHHCCCCCHHHHCCCEEEEECCCCHHHCCCCCHHHHHHHHHHH
EINSYKQLPLNFYQIQTKFRDEIRPRFGVMRAREFLMKDAYSFHLTDEDMAREYDNMRAA
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCHHHHHHHHHHHHH
YTRIFTRLGLDFRAVQADSGAIGGDASQEFHVIADSGEDSLAFSTGSDYAANVEAASAAP
HHHHHHHHCCCEEEEECCCCCCCCCCCCCEEEEECCCCCCEEEECCCCCCCCCCCCCCCC
PAPRAAASEAMQQVATPTQKTCEDVAQLLGIALQRTVKSVAVMTQAGFVLVLVRGDHAVN
CCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCC
EIKLAKVPGLADYRLANESEIREHLGCEPGFLGPVNTARPVRVVADRDVAALADFVVGAN
CEEEEECCCCCHHCCCCHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHCCC
VSGAHLVGVNWGRDLPEPETVADVRNVVEGERAADGGELRLARGIEVGHVFQLGSQYAQA
CCCCEEEEECCCCCCCCCHHHHHHHHHHCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHH
LQATVIDEGGKAAVMKMGCYGIGISRIVAAAIEQNHDDAGIIWPAPMAPWQVVVCVINPK
HHHHHHCCCCCEEEEEECCHHCCHHHHHHHHHHCCCCCCCEEECCCCCCCEEEEEEECCC
QDPQVLAAAQALLDQLLAAGLDAALDDRGLRPGAMFADMELLGIPHRVVVSERGLAAGTF
CCHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCHHHCHHHHCCCCEEEEECCCCCCCCH
EYRARTADAAESLDKAGLFSRLGH
HHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRLSQFHLHTTKETPADAELVSHRLMLRAGMIRKLASGLYTWSPLGLRVLRKVEAIVREE
CCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHH
MNRAGAVEVLFPTIQPRELWDATGRWEKFGGQLLKIKDRKEQEFCYSPTAEEAAAEFARQ
HHHCCCEEEEECCCCCHHHHCCCCCHHHHCCCEEEEECCCCHHHCCCCCHHHHHHHHHHH
EINSYKQLPLNFYQIQTKFRDEIRPRFGVMRAREFLMKDAYSFHLTDEDMAREYDNMRAA
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCHHHHHHHHHHHHH
YTRIFTRLGLDFRAVQADSGAIGGDASQEFHVIADSGEDSLAFSTGSDYAANVEAASAAP
HHHHHHHHCCCEEEEECCCCCCCCCCCCCEEEEECCCCCCEEEECCCCCCCCCCCCCCCC
PAPRAAASEAMQQVATPTQKTCEDVAQLLGIALQRTVKSVAVMTQAGFVLVLVRGDHAVN
CCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCC
EIKLAKVPGLADYRLANESEIREHLGCEPGFLGPVNTARPVRVVADRDVAALADFVVGAN
CEEEEECCCCCHHCCCCHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHCCC
VSGAHLVGVNWGRDLPEPETVADVRNVVEGERAADGGELRLARGIEVGHVFQLGSQYAQA
CCCCEEEEECCCCCCCCCHHHHHHHHHHCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHH
LQATVIDEGGKAAVMKMGCYGIGISRIVAAAIEQNHDDAGIIWPAPMAPWQVVVCVINPK
HHHHHHCCCCCEEEEEECCHHCCHHHHHHHHHHCCCCCCCEEECCCCCCCEEEEEEECCC
QDPQVLAAAQALLDQLLAAGLDAALDDRGLRPGAMFADMELLGIPHRVVVSERGLAAGTF
CCHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCHHHCHHHHCCCCEEEEECCCCCCCCH
EYRARTADAAESLDKAGLFSRLGH
HHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12024217