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The map label for this gene is dadA [C]

Identifier: 77748662

GI number: 77748662

Start: 3007174

End: 3008451

Strand: Reverse

Name: dadA [C]

Synonym: XAC2549

Alternate gene names: 77748662

Gene position: 3008451-3007174 (Counterclockwise)

Preceding gene: 21243283

Following gene: 21243281

Centisome position: 58.13

GC content: 71.36

Gene sequence:

>1278_bases
ATGGAACCCCAGCGGCCCGCGCCGGTGCTTGCGTCGGTCACGCCGTCTGGCGCAGCAGTGTCTGCTGCGATGACAGGGAC
TGCCCCTGCAACGCCTGCGTCGGTATCGGCGCCTGGATTGGATGCGGTGACCGAATCCGGGTCCGCCGCGTCTGCCGCAG
GCACGCGACGCAGTTACGACCTGATCGTGATCGGCGCCGGCATTGTGGGCGCCGCGTGCGCGGAAGCGGCGGCGGGCGAG
GGCCTTCGTGTGGCGATCGTCGAGCCGGGACCGATCGGCGGCGGCTCCACCGCAGCGGCGATGGGGCATTTGGTGGCGAT
GGACGACGACCCGGCCGAGCTGGCGTTGTCGGCGTATTCGCTGCGGCTGTGGGAGCGCTTCGCCACGCTTAGCGAAGCCG
AATTCAGCCGCTGCGGCACGCTGTGGGTGGCCCGCGATGCGCGCGAACTGGCGGCGGTGCCAGCCAAGATCGCACGCCTG
GCAACGGCCGGCCTGCATGCCGAAGCGATCGATGCCGCGCAGCTGTACGCGCTGGAACCACAGCTGGTGCCCGGGCTGGC
TGGCGGCATGCGCGTGCCCGATGAAGCCGTGGTGTATCCGCCGCGGGTCGCGCGGCATTTGGTGGGCGCGGCATGCACGT
CCGGTGCGCGCTTGTTCGCCGGCCGCCGCGTGGTGCAGCTGCAGGAGCATGCGGTGCGTCTGGACGATGGGCAGACGCTC
GCCGGGCCGGTGCTGGTGGCCAGCGGTGTGGCCTTGCCGCAGTTGCTGCCCGAGCTGGCGCTGCGCCCGCGCAAGGGCCA
TTTGGTGATTACCGACCGGCACCCGGGGCTGATCCGGCATCAGCTGCTGGAACTGGGCTATGCGGATTCGGCGCATGGAG
CCGACGACACCAGCGTGGCCTTCAATGTGCAGCCGCGGCCCACCGGGCAGATTCTGATCGGCTCCTCGCGCCAGTTCGGC
GAGCACGACCGCGCCTTGTCGATGCCGGTGCTGCAGCAGATGCTGCAGCGCGCCTTCGCGTACCTGCCAGTGCTGCGCGA
ACTGCAAGCGATCCGCGTGTGGACCGGTTTGCGACCGGCCACGCCGGACGGGCGCCCGTATTTGGGGAAGGTGCCCGGCC
GGCGCGATGTGTGGGTGGCCGCCGGTCACGAGGGGCTTGGGGTCACCACGGCGCTGGGGAGCGCACGGGTGATCGTGGAC
AGCCTGCTCGGGCGCACCCCGGCCATCGACCCGGCGCCCTACGCACCGGCGCGTGCCGCGCAAGGAGCGCCCGCATGA

Upstream 100 bases:

>100_bases
GCGGCATCGCACCGCGCATCAGTGGGCGGGCGTACATCACCGCGCGCGCGCAACTGGTGATCGACCCGGCGGACCCGTTC
GCCTGGGGCATCGTGGCCTG

Downstream 100 bases:

>100_bases
GCGCGACGGTGCGTCTGCACGTGGATGGGCGCATGGTCGAGGTGCCGGCCGGTGCCAGCGTGGCGGCGGCAGTGGCGCAG
GCGACGTTGCAGTTTCGGCA

Product: D-amino acid oxidase

Products: FADH2; ammonia; Pyruvate [C]

Alternate protein names: NA

Number of amino acids: Translated: 425; Mature: 425

Protein sequence:

>425_residues
MEPQRPAPVLASVTPSGAAVSAAMTGTAPATPASVSAPGLDAVTESGSAASAAGTRRSYDLIVIGAGIVGAACAEAAAGE
GLRVAIVEPGPIGGGSTAAAMGHLVAMDDDPAELALSAYSLRLWERFATLSEAEFSRCGTLWVARDARELAAVPAKIARL
ATAGLHAEAIDAAQLYALEPQLVPGLAGGMRVPDEAVVYPPRVARHLVGAACTSGARLFAGRRVVQLQEHAVRLDDGQTL
AGPVLVASGVALPQLLPELALRPRKGHLVITDRHPGLIRHQLLELGYADSAHGADDTSVAFNVQPRPTGQILIGSSRQFG
EHDRALSMPVLQQMLQRAFAYLPVLRELQAIRVWTGLRPATPDGRPYLGKVPGRRDVWVAAGHEGLGVTTALGSARVIVD
SLLGRTPAIDPAPYAPARAAQGAPA

Sequences:

>Translated_425_residues
MEPQRPAPVLASVTPSGAAVSAAMTGTAPATPASVSAPGLDAVTESGSAASAAGTRRSYDLIVIGAGIVGAACAEAAAGE
GLRVAIVEPGPIGGGSTAAAMGHLVAMDDDPAELALSAYSLRLWERFATLSEAEFSRCGTLWVARDARELAAVPAKIARL
ATAGLHAEAIDAAQLYALEPQLVPGLAGGMRVPDEAVVYPPRVARHLVGAACTSGARLFAGRRVVQLQEHAVRLDDGQTL
AGPVLVASGVALPQLLPELALRPRKGHLVITDRHPGLIRHQLLELGYADSAHGADDTSVAFNVQPRPTGQILIGSSRQFG
EHDRALSMPVLQQMLQRAFAYLPVLRELQAIRVWTGLRPATPDGRPYLGKVPGRRDVWVAAGHEGLGVTTALGSARVIVD
SLLGRTPAIDPAPYAPARAAQGAPA
>Mature_425_residues
MEPQRPAPVLASVTPSGAAVSAAMTGTAPATPASVSAPGLDAVTESGSAASAAGTRRSYDLIVIGAGIVGAACAEAAAGE
GLRVAIVEPGPIGGGSTAAAMGHLVAMDDDPAELALSAYSLRLWERFATLSEAEFSRCGTLWVARDARELAAVPAKIARL
ATAGLHAEAIDAAQLYALEPQLVPGLAGGMRVPDEAVVYPPRVARHLVGAACTSGARLFAGRRVVQLQEHAVRLDDGQTL
AGPVLVASGVALPQLLPELALRPRKGHLVITDRHPGLIRHQLLELGYADSAHGADDTSVAFNVQPRPTGQILIGSSRQFG
EHDRALSMPVLQQMLQRAFAYLPVLRELQAIRVWTGLRPATPDGRPYLGKVPGRRDVWVAAGHEGLGVTTALGSARVIVD
SLLGRTPAIDPAPYAPARAAQGAPA

Specific function: Oxidative Deamination Of D-Amino Acids. [C]

COG id: COG0665

COG function: function code E; Glycine/D-amino acid oxidases (deaminating)

Gene ontology:

Cell location: Inner Membrane-Bound [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DadA oxidoreductase family [H]

Homologues:

Organism=Escherichia coli, GI1787438, Length=309, Percent_Identity=24.5954692556634, Blast_Score=72, Evalue=8e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006076
- InterPro:   IPR012727 [H]

Pfam domain/function: PF01266 DAO [H]

EC number: 1.4.99.1 [C]

Molecular weight: Translated: 43846; Mature: 43846

Theoretical pI: Translated: 7.35; Mature: 7.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEPQRPAPVLASVTPSGAAVSAAMTGTAPATPASVSAPGLDAVTESGSAASAAGTRRSYD
CCCCCCCCEEEECCCCCCEEEEEECCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCEE
LIVIGAGIVGAACAEAAAGEGLRVAIVEPGPIGGGSTAAAMGHLVAMDDDPAELALSAYS
EEEECCCHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHCEEEEECCCHHHHHHHHHH
LRLWERFATLSEAEFSRCGTLWVARDARELAAVPAKIARLATAGLHAEAIDAAQLYALEP
HHHHHHHHHHHHHHHHHCCEEEEECCHHHHHHHHHHHHHHHHHCCCHHHHCHHHEEEECC
QLVPGLAGGMRVPDEAVVYPPRVARHLVGAACTSGARLFAGRRVVQLQEHAVRLDDGQTL
CCCCCCCCCCCCCCCCEECCHHHHHHHHHHHHCCCCHHHHCCHHEEEHHHHEECCCCCCC
AGPVLVASGVALPQLLPELALRPRKGHLVITDRHPGLIRHQLLELGYADSAHGADDTSVA
CCHHHHHCCCHHHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHHHCCCCCCCCCCCCEEE
FNVQPRPTGQILIGSSRQFGEHDRALSMPVLQQMLQRAFAYLPVLRELQAIRVWTGLRPA
EEECCCCCCEEEECCCCCCCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
TPDGRPYLGKVPGRRDVWVAAGHEGLGVTTALGSARVIVDSLLGRTPAIDPAPYAPARAA
CCCCCCCCCCCCCCCCEEEEECCCCCEEEECCCCHHHHHHHHHCCCCCCCCCCCCCCCCC
QGAPA
CCCCC
>Mature Secondary Structure
MEPQRPAPVLASVTPSGAAVSAAMTGTAPATPASVSAPGLDAVTESGSAASAAGTRRSYD
CCCCCCCCEEEECCCCCCEEEEEECCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCEE
LIVIGAGIVGAACAEAAAGEGLRVAIVEPGPIGGGSTAAAMGHLVAMDDDPAELALSAYS
EEEECCCHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHCEEEEECCCHHHHHHHHHH
LRLWERFATLSEAEFSRCGTLWVARDARELAAVPAKIARLATAGLHAEAIDAAQLYALEP
HHHHHHHHHHHHHHHHHCCEEEEECCHHHHHHHHHHHHHHHHHCCCHHHHCHHHEEEECC
QLVPGLAGGMRVPDEAVVYPPRVARHLVGAACTSGARLFAGRRVVQLQEHAVRLDDGQTL
CCCCCCCCCCCCCCCCEECCHHHHHHHHHHHHCCCCHHHHCCHHEEEHHHHEECCCCCCC
AGPVLVASGVALPQLLPELALRPRKGHLVITDRHPGLIRHQLLELGYADSAHGADDTSVA
CCHHHHHCCCHHHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHHHCCCCCCCCCCCCEEE
FNVQPRPTGQILIGSSRQFGEHDRALSMPVLQQMLQRAFAYLPVLRELQAIRVWTGLRPA
EEECCCCCCEEEECCCCCCCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
TPDGRPYLGKVPGRRDVWVAAGHEGLGVTTALGSARVIVDSLLGRTPAIDPAPYAPARAA
CCCCCCCCCCCCCCCCEEEEECCCCCEEEECCCCHHHHHHHHHCCCCCCCCCCCCCCCCC
QGAPA
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Iron [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 40 {D-proline}} 0.13 {K3Fe(CN)6}} 11 {D-methionine}} 6 {D-phenylalanine}} 12 {D-alpha-aminobutyrate}} 14 {D-glutamine}} 0.63 {dichloroindophenol}} 0.01 {dichloroindophenol}} 0.15 {cytochrome} 19 {D-serine}} 29 {D-alanine}} 6.4

Substrates: D-Alanine; FAD; H2O [C]

Specific reaction: D-Alanine + FAD + H2O --> FADH2 + ammonia + Pyruvate [C]

General reaction: Oxidative deamination; Redox reaction [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8682785; 10984043; 8097014 [H]