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The map label for this gene is potH [H]

Identifier: 77748657

GI number: 77748657

Start: 2875826

End: 2876737

Strand: Reverse

Name: potH [H]

Synonym: XAC2471

Alternate gene names: 77748657

Gene position: 2876737-2875826 (Counterclockwise)

Preceding gene: 21243205

Following gene: 21243203

Centisome position: 55.58

GC content: 61.84

Gene sequence:

>912_bases
ATGAAACGCGTGTTCATACGCGGGTTAGCGGGCGCGCGCTGGGGCGTGATCGCCGCACCATATCTGTGGATGCTGGTGTT
CTTCGCGATTCCGTTCCTGATCGTGCTGAAGATTTCCTTCGCCGAGCGCGCCACCGCGATGCCGCCGTATACGCCGCTAT
TGGCGGTTGCTGCCGACGGTGCGGTGAGCCTCAAACTGCATCTGGGCGCCTATCTGGCCTTGCTGCGCGATAGCCAGTAC
GTGGCTGCCTACCTGAGTTCGATCAAGATCGCGGCCATTTCCACCGCACTGGCGCTGTTGATCGGCTACCCGATGGCCTA
CGTGATCGCGCGGCTACCGCTGGCCACACGCAACGTGGCGATGATGCTGGTGGTGCTGCCGTCGTGGACGTCGTTTCTGA
TCCGCGTGTACGCGTGGATCGGCATTCTCGATGGCAATGGTGTGCTCAATCAGGCGCTGTTGGCGCTGGGTGTGATCGAG
CAGCCGTTGCAATTGTTGTACACGCCGATCGCCGCTTACATCGGCATCGTGTACTGCTATCTGCCGTTCATGGTGTTGCC
GCTGTACGCCAACCTGGTCAAGCACGATCAGCGCCTGCTCGAAGCAGCCTACGACCTGGGTGCGCGGCCATGGCAGGCAT
TCGTCCGCATCACCTTGCCACTATCGCGCAACGGCATCGTGGCCGGCTGCATGCTGGTGATGATTCCGGCGGTCGGCGAG
TTCGTCATCCCGGAAATGCTGGGCGGGCCGAACACCTTGATGATCGGGCGCGTGCTGTGGGGCGAGTTCTTCAATAACCG
CGACTGGCCGGTGGCAGCGGCAGTGGCCACGGTGATGTTGCTGCTGCTGTTGCTGCCGATCGTGATCTTCCACCGTTACC
AGCAGCGTGAGCTGGAAGGGCGCCTGACATGA

Upstream 100 bases:

>100_bases
CCAATTTCGCCAATCGCCAGCGTTGGGCCAGCGAAGCGTTGACCTGGGGCGACACCGTGTGGGTAGGGTGGGGCGAAGAC
GACGGCGTGGTGTTCACCGC

Downstream 100 bases:

>100_bases
TGCGCGCGTCGCGCGGTGGGCGCCTGCTCGGCGGCAGCGTGCTCGCGCTGGGTTTCGGCTTCTTGTACCTGCCGATCCTG
TTGTTGATGGTGTATTCGTT

Product: polyamine transport protein

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 303; Mature: 303

Protein sequence:

>303_residues
MKRVFIRGLAGARWGVIAAPYLWMLVFFAIPFLIVLKISFAERATAMPPYTPLLAVAADGAVSLKLHLGAYLALLRDSQY
VAAYLSSIKIAAISTALALLIGYPMAYVIARLPLATRNVAMMLVVLPSWTSFLIRVYAWIGILDGNGVLNQALLALGVIE
QPLQLLYTPIAAYIGIVYCYLPFMVLPLYANLVKHDQRLLEAAYDLGARPWQAFVRITLPLSRNGIVAGCMLVMIPAVGE
FVIPEMLGGPNTLMIGRVLWGEFFNNRDWPVAAAVATVMLLLLLLPIVIFHRYQQRELEGRLT

Sequences:

>Translated_303_residues
MKRVFIRGLAGARWGVIAAPYLWMLVFFAIPFLIVLKISFAERATAMPPYTPLLAVAADGAVSLKLHLGAYLALLRDSQY
VAAYLSSIKIAAISTALALLIGYPMAYVIARLPLATRNVAMMLVVLPSWTSFLIRVYAWIGILDGNGVLNQALLALGVIE
QPLQLLYTPIAAYIGIVYCYLPFMVLPLYANLVKHDQRLLEAAYDLGARPWQAFVRITLPLSRNGIVAGCMLVMIPAVGE
FVIPEMLGGPNTLMIGRVLWGEFFNNRDWPVAAAVATVMLLLLLLPIVIFHRYQQRELEGRLT
>Mature_303_residues
MKRVFIRGLAGARWGVIAAPYLWMLVFFAIPFLIVLKISFAERATAMPPYTPLLAVAADGAVSLKLHLGAYLALLRDSQY
VAAYLSSIKIAAISTALALLIGYPMAYVIARLPLATRNVAMMLVVLPSWTSFLIRVYAWIGILDGNGVLNQALLALGVIE
QPLQLLYTPIAAYIGIVYCYLPFMVLPLYANLVKHDQRLLEAAYDLGARPWQAFVRITLPLSRNGIVAGCMLVMIPAVGE
FVIPEMLGGPNTLMIGRVLWGEFFNNRDWPVAAAVATVMLLLLLLPIVIFHRYQQRELEGRLT

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine [H]

COG id: COG1176

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component I

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787080, Length=283, Percent_Identity=61.4840989399293, Blast_Score=331, Evalue=4e-92,
Organism=Escherichia coli, GI1787369, Length=282, Percent_Identity=39.3617021276596, Blast_Score=198, Evalue=5e-52,
Organism=Escherichia coli, GI1787713, Length=246, Percent_Identity=29.6747967479675, Blast_Score=96, Evalue=3e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 33473; Mature: 33473

Theoretical pI: Translated: 9.76; Mature: 9.76

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRVFIRGLAGARWGVIAAPYLWMLVFFAIPFLIVLKISFAERATAMPPYTPLLAVAADG
CCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHEECCC
AVSLKLHLGAYLALLRDSQYVAAYLSSIKIAAISTALALLIGYPMAYVIARLPLATRNVA
CEEEEHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHH
MMLVVLPSWTSFLIRVYAWIGILDGNGVLNQALLALGVIEQPLQLLYTPIAAYIGIVYCY
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LPFMVLPLYANLVKHDQRLLEAAYDLGARPWQAFVRITLPLSRNGIVAGCMLVMIPAVGE
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHEEEEECCCCCCHHHHHHHHHHHHHHH
FVIPEMLGGPNTLMIGRVLWGEFFNNRDWPVAAAVATVMLLLLLLPIVIFHRYQQRELEG
HHHHHHCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
RLT
CCC
>Mature Secondary Structure
MKRVFIRGLAGARWGVIAAPYLWMLVFFAIPFLIVLKISFAERATAMPPYTPLLAVAADG
CCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHEECCC
AVSLKLHLGAYLALLRDSQYVAAYLSSIKIAAISTALALLIGYPMAYVIARLPLATRNVA
CEEEEHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHH
MMLVVLPSWTSFLIRVYAWIGILDGNGVLNQALLALGVIEQPLQLLYTPIAAYIGIVYCY
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LPFMVLPLYANLVKHDQRLLEAAYDLGARPWQAFVRITLPLSRNGIVAGCMLVMIPAVGE
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHEEEEECCCCCCHHHHHHHHHHHHHHH
FVIPEMLGGPNTLMIGRVLWGEFFNNRDWPVAAAVATVMLLLLLLPIVIFHRYQQRELEG
HHHHHHCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
RLT
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8416922; 8905232; 9278503 [H]