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The map label for this gene is potI [H]

Identifier: 21243203

GI number: 21243203

Start: 2874981

End: 2875829

Strand: Reverse

Name: potI [H]

Synonym: XAC2470

Alternate gene names: 21243203

Gene position: 2875829-2874981 (Counterclockwise)

Preceding gene: 77748657

Following gene: 21243202

Centisome position: 55.57

GC content: 63.84

Gene sequence:

>849_bases
ATGATGCGCGCGTCGCGCGGTGGGCGCCTGCTCGGCGGCAGCGTGCTCGCGCTGGGTTTCGGCTTCTTGTACCTGCCGAT
CCTGTTGTTGATGGTGTATTCGTTCAACGCCTCGCGCCTGGCGACGGTGTGGGGCGGGTTCTCGGCCAGGTGGTACGGCG
AATTAGTGCGCGACCGGCAATTGCTCGACGCAGCCTGGGTCAGCCTGGAGGTGGCGTTCTGGACGGCCTGCGCATCGACG
GTGCTGGGCACGATGGCCGCCATGGCGATGGTGCGCATGCGGCGTTTTCCCGGCAAAACCCTGTTCGGCGCACTGATCAC
CGCGCCGTTGGTGATGCCCGAAGTCATCATCGGTCTGTCGATCCTGCTGTTGTTGGTGTCGATGGGTGGCGTGCTCGGCA
TCGCGCCGCGCGGCGCGGTAGCGATCTGGGTAGCGCATGTCACCTTCACCGTATCGTTCGTGACCGTGGTGATTTCTTCG
CGGCTGCAGGAACTGGACCGCTCGCTTGAAGAAGCCGCGATGGATCTCGGTGCCACGCCGCTGAAAGTGTTTTTCCAGAT
CACCTTGCCGATCATCGCGCCGGCCCTGGCATCGGGCTGGCTGCTGGCCTTTACGCTGTCGCTGGACGATGTGGTGATTG
CCAGTTTCCTGGCCGGGCCCAGTTCCACCACCTTGCCGATCAAGGTGTTTTCGTCGGTGCGTCTGGGCATCAGTCCGAAG
ATCAATGCGCTGGCCACGCTTATGGTGCTGGCGGTGTCGGTAGCCGCGCTGATCGGCTGGTGGCTGCTGGCGCGCAGCGA
AAAACGTCGCCTGCGCGATGCGCAGTTGGCACTGCAACGCGAAGGTTGA

Upstream 100 bases:

>100_bases
CGGTGGCAGCGGCAGTGGCCACGGTGATGTTGCTGCTGCTGTTGCTGCCGATCGTGATCTTCCACCGTTACCAGCAGCGT
GAGCTGGAAGGGCGCCTGAC

Downstream 100 bases:

>100_bases
GCGAGACAGGAAGGCGCGAGGCGTAGATGCGTGCAGCGGCGGGTGACCGTTGGGCAGGGCAGCTTCCGAAACCAGTGCGA
CTGCATGCACCAGGTGGCGC

Product: putrescine transport protein

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 282; Mature: 282

Protein sequence:

>282_residues
MMRASRGGRLLGGSVLALGFGFLYLPILLLMVYSFNASRLATVWGGFSARWYGELVRDRQLLDAAWVSLEVAFWTACAST
VLGTMAAMAMVRMRRFPGKTLFGALITAPLVMPEVIIGLSILLLLVSMGGVLGIAPRGAVAIWVAHVTFTVSFVTVVISS
RLQELDRSLEEAAMDLGATPLKVFFQITLPIIAPALASGWLLAFTLSLDDVVIASFLAGPSSTTLPIKVFSSVRLGISPK
INALATLMVLAVSVAALIGWWLLARSEKRRLRDAQLALQREG

Sequences:

>Translated_282_residues
MMRASRGGRLLGGSVLALGFGFLYLPILLLMVYSFNASRLATVWGGFSARWYGELVRDRQLLDAAWVSLEVAFWTACAST
VLGTMAAMAMVRMRRFPGKTLFGALITAPLVMPEVIIGLSILLLLVSMGGVLGIAPRGAVAIWVAHVTFTVSFVTVVISS
RLQELDRSLEEAAMDLGATPLKVFFQITLPIIAPALASGWLLAFTLSLDDVVIASFLAGPSSTTLPIKVFSSVRLGISPK
INALATLMVLAVSVAALIGWWLLARSEKRRLRDAQLALQREG
>Mature_282_residues
MMRASRGGRLLGGSVLALGFGFLYLPILLLMVYSFNASRLATVWGGFSARWYGELVRDRQLLDAAWVSLEVAFWTACAST
VLGTMAAMAMVRMRRFPGKTLFGALITAPLVMPEVIIGLSILLLLVSMGGVLGIAPRGAVAIWVAHVTFTVSFVTVVISS
RLQELDRSLEEAAMDLGATPLKVFFQITLPIIAPALASGWLLAFTLSLDDVVIASFLAGPSSTTLPIKVFSSVRLGISPK
INALATLMVLAVSVAALIGWWLLARSEKRRLRDAQLALQREG

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine [H]

COG id: COG1177

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component II

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787081, Length=264, Percent_Identity=61.7424242424242, Blast_Score=298, Evalue=2e-82,
Organism=Escherichia coli, GI1787368, Length=262, Percent_Identity=39.6946564885496, Blast_Score=182, Evalue=2e-47,
Organism=Escherichia coli, GI1787714, Length=253, Percent_Identity=33.596837944664, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1788764, Length=176, Percent_Identity=34.0909090909091, Blast_Score=75, Evalue=6e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 30322; Mature: 30322

Theoretical pI: Translated: 11.15; Mature: 11.15

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMRASRGGRLLGGSVLALGFGFLYLPILLLMVYSFNASRLATVWGGFSARWYGELVRDRQ
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCHHHHHHHHHHHH
LLDAAWVSLEVAFWTACASTVLGTMAAMAMVRMRRFPGKTLFGALITAPLVMPEVIIGLS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
ILLLLVSMGGVLGIAPRGAVAIWVAHVTFTVSFVTVVISSRLQELDRSLEEAAMDLGATP
HHHHHHHHCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
LKVFFQITLPIIAPALASGWLLAFTLSLDDVVIASFLAGPSSTTLPIKVFSSVRLGISPK
HHHHHHHHHHHHHHHHHCCHHHHEEHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCC
INALATLMVLAVSVAALIGWWLLARSEKRRLRDAQLALQREG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MMRASRGGRLLGGSVLALGFGFLYLPILLLMVYSFNASRLATVWGGFSARWYGELVRDRQ
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCHHHHHHHHHHHH
LLDAAWVSLEVAFWTACASTVLGTMAAMAMVRMRRFPGKTLFGALITAPLVMPEVIIGLS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
ILLLLVSMGGVLGIAPRGAVAIWVAHVTFTVSFVTVVISSRLQELDRSLEEAAMDLGATP
HHHHHHHHCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
LKVFFQITLPIIAPALASGWLLAFTLSLDDVVIASFLAGPSSTTLPIKVFSSVRLGISPK
HHHHHHHHHHHHHHHHHCCHHHHEEHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCC
INALATLMVLAVSVAALIGWWLLARSEKRRLRDAQLALQREG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]