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The map label for this gene is miaB [H]

Identifier: 77748655

GI number: 77748655

Start: 2864991

End: 2866445

Strand: Direct

Name: miaB [H]

Synonym: XAC2461

Alternate gene names: 77748655

Gene position: 2864991-2866445 (Clockwise)

Preceding gene: 21243193

Following gene: 21243195

Centisome position: 55.36

GC content: 63.78

Gene sequence:

>1455_bases
ATGCCCGGGACACCCGTTTCCGACCTGTCCGCGGCCACTGCCGTGGACGCAACCGCCCTGTTGCCCTTGCCCGTCGCGCG
CCCATCGGCGCCTGCCATGGTGCGCGGCAAGCTCTATATCAAGACCCACGGATGCCAGATGAACGAGTACGACTCGGCCA
AGATGGCCGACGTGCTTGCCGCCTCCGAAGGGCTGGAGCTGACCGACGATCCGGAAGAAGCGGATGTGGTGCTGGTCAAC
ACCTGCTCCATTCGCGAAAAGGCGCAGGAAAAGGTCTTCAGCCAGCTGGGTCGCTGGAAGGCGCTGAAGGCCGGCGGCAA
GCCGGTGATCATCGGCGTCGGCGGCTGCGTGGCATCGCAGGAAGGCGAAGCCATCGTCAAGCGCGCGCCCTACGTGGACC
TGGTGTTCGGTCCGCAAACGCTGCATCGGCTGCCGGAGCTGATCCGCGCGCGGCGCGAATCGGGCAAGTCGCAGGTGGAC
ATCAGCTTCCCGGAGATCGAGAAGTTCGACCGCCTGCCCGAGCCGCGTGCCGAGGGCCCCTCGGCGTTCGTGTCGATCAT
GGAAGGCTGCTCCAAATACTGCTCGTTCTGCGTGGTGCCCTACACCCGTGGCGAAGAAGTCAGCCGGCCGTTCGAAGACG
TGCTGGTGGAAGTGGCGCAATTGGCAGCGCAAGGCGTGCGCGAGATCAACCTGCTCGGCCAGAACGTCAATGCGTATCGC
GGTGCGTATGGCGCCGATGCCGGCGACGCCGCGCAGTACGCCGATCTGGGCCTGCTGATCCGCACCATCGCGCAGATCGA
GGGCATCGGCCGCATCCGCTTCACCACCTCGCATCCGCTGGAGTTTTCCGATTCGCTGGTGGATGCCTACCGCGATGTGC
CGCAGCTGGCCAACTACCTGCACCTGCCGGTGCAGGCCGGCAGCGACCGCATTCTGTCGGCGATGAAGCGTGGCTACACC
GCGCTGGAATTCAAGTCGAAGATCCGCAAGCTGCGCGCAGTGCGCCCGGACATCTCGATCAGTTCGGACTTCATCGTCGG
CTTTCCTGGCGAGACCGAAGCCGACTTCGAAAAGACCATGAAGCTGATCGAAGACGTAGGCTTCGACCAGAGCTTTTCGT
TCGTGTATTCGCGTCGCCCCGGCACCCCGGCATCGGACCTGCAGGACGACACGCCGGAGGCAGTGAAGCAGGCGCGTCTT
GCGCGTCTGCAGGCGCATATCAATGCGCACGCGGCGAGCATTTCGCAATCGATGGTGGGCAGCGTGCAGCGCGTGCTGGT
GGAAGGCCCCTCGCGGCGCGATCCGAACGAATTGACCGGCAAGAGCGAAAACATGCGCCCGGTGAATTTCCCGGGCAACC
CACGTTTGATCGGACAGTTCGTCGATGTGTTGATCACCGAGGCAATGAGCAATTCGCTGCGTGGTCGGATTCAATTGGAC
GACAGCGCACATTGA

Upstream 100 bases:

>100_bases
CGCGCGCCGAAGCCGTCGACGCCGGGCGCGCGCCCGCGGACGGCGCCAGCGCAAGCGGGTAAACTGCGCGGCTCTCTTCT
ACCCGCCTGGAATAAGCGCC

Downstream 100 bases:

>100_bases
TGGCGCGTGGGCGCTGCGCAATGCGGCGCTCCTCCAAGCAAGGCAACCACGCGCCGAAGCGTGTCGCGCGAAATGGTCAA
GATTTCACCACACAGCTGTC

Product: (dimethylallyl)adenosine tRNA methylthiotransferase

Products: NA

Alternate protein names: tRNA-i(6)A37 methylthiotransferase [H]

Number of amino acids: Translated: 484; Mature: 483

Protein sequence:

>484_residues
MPGTPVSDLSAATAVDATALLPLPVARPSAPAMVRGKLYIKTHGCQMNEYDSAKMADVLAASEGLELTDDPEEADVVLVN
TCSIREKAQEKVFSQLGRWKALKAGGKPVIIGVGGCVASQEGEAIVKRAPYVDLVFGPQTLHRLPELIRARRESGKSQVD
ISFPEIEKFDRLPEPRAEGPSAFVSIMEGCSKYCSFCVVPYTRGEEVSRPFEDVLVEVAQLAAQGVREINLLGQNVNAYR
GAYGADAGDAAQYADLGLLIRTIAQIEGIGRIRFTTSHPLEFSDSLVDAYRDVPQLANYLHLPVQAGSDRILSAMKRGYT
ALEFKSKIRKLRAVRPDISISSDFIVGFPGETEADFEKTMKLIEDVGFDQSFSFVYSRRPGTPASDLQDDTPEAVKQARL
ARLQAHINAHAASISQSMVGSVQRVLVEGPSRRDPNELTGKSENMRPVNFPGNPRLIGQFVDVLITEAMSNSLRGRIQLD
DSAH

Sequences:

>Translated_484_residues
MPGTPVSDLSAATAVDATALLPLPVARPSAPAMVRGKLYIKTHGCQMNEYDSAKMADVLAASEGLELTDDPEEADVVLVN
TCSIREKAQEKVFSQLGRWKALKAGGKPVIIGVGGCVASQEGEAIVKRAPYVDLVFGPQTLHRLPELIRARRESGKSQVD
ISFPEIEKFDRLPEPRAEGPSAFVSIMEGCSKYCSFCVVPYTRGEEVSRPFEDVLVEVAQLAAQGVREINLLGQNVNAYR
GAYGADAGDAAQYADLGLLIRTIAQIEGIGRIRFTTSHPLEFSDSLVDAYRDVPQLANYLHLPVQAGSDRILSAMKRGYT
ALEFKSKIRKLRAVRPDISISSDFIVGFPGETEADFEKTMKLIEDVGFDQSFSFVYSRRPGTPASDLQDDTPEAVKQARL
ARLQAHINAHAASISQSMVGSVQRVLVEGPSRRDPNELTGKSENMRPVNFPGNPRLIGQFVDVLITEAMSNSLRGRIQLD
DSAH
>Mature_483_residues
PGTPVSDLSAATAVDATALLPLPVARPSAPAMVRGKLYIKTHGCQMNEYDSAKMADVLAASEGLELTDDPEEADVVLVNT
CSIREKAQEKVFSQLGRWKALKAGGKPVIIGVGGCVASQEGEAIVKRAPYVDLVFGPQTLHRLPELIRARRESGKSQVDI
SFPEIEKFDRLPEPRAEGPSAFVSIMEGCSKYCSFCVVPYTRGEEVSRPFEDVLVEVAQLAAQGVREINLLGQNVNAYRG
AYGADAGDAAQYADLGLLIRTIAQIEGIGRIRFTTSHPLEFSDSLVDAYRDVPQLANYLHLPVQAGSDRILSAMKRGYTA
LEFKSKIRKLRAVRPDISISSDFIVGFPGETEADFEKTMKLIEDVGFDQSFSFVYSRRPGTPASDLQDDTPEAVKQARLA
RLQAHINAHAASISQSMVGSVQRVLVEGPSRRDPNELTGKSENMRPVNFPGNPRLIGQFVDVLITEAMSNSLRGRIQLDD
SAH

Specific function: Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine [H]

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI28872784, Length=476, Percent_Identity=38.0252100840336, Blast_Score=301, Evalue=1e-81,
Organism=Homo sapiens, GI28872782, Length=476, Percent_Identity=38.0252100840336, Blast_Score=300, Evalue=2e-81,
Organism=Homo sapiens, GI93277076, Length=452, Percent_Identity=28.9823008849558, Blast_Score=171, Evalue=1e-42,
Organism=Escherichia coli, GI1786882, Length=441, Percent_Identity=67.1201814058957, Blast_Score=619, Evalue=1e-178,
Organism=Escherichia coli, GI1787057, Length=374, Percent_Identity=29.9465240641711, Blast_Score=156, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI17553146, Length=460, Percent_Identity=34.7826086956522, Blast_Score=259, Evalue=3e-69,
Organism=Caenorhabditis elegans, GI71996771, Length=308, Percent_Identity=30.5194805194805, Blast_Score=115, Evalue=4e-26,
Organism=Drosophila melanogaster, GI21356207, Length=474, Percent_Identity=33.7552742616034, Blast_Score=285, Evalue=5e-77,
Organism=Drosophila melanogaster, GI19922432, Length=459, Percent_Identity=30.718954248366, Blast_Score=165, Evalue=7e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006463
- InterPro:   IPR007197
- InterPro:   IPR002792 [H]

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004 [H]

EC number: NA

Molecular weight: Translated: 52703; Mature: 52572

Theoretical pI: Translated: 5.57; Mature: 5.57

Prosite motif: PS50926 TRAM ; PS01278 UPF0004

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPGTPVSDLSAATAVDATALLPLPVARPSAPAMVRGKLYIKTHGCQMNEYDSAKMADVLA
CCCCCCCHHHHHHHHCHHHCCCCCCCCCCCCCEEEEEEEEEECCCCCCCCCCHHHHHHHH
ASEGLELTDDPEEADVVLVNTCSIREKAQEKVFSQLGRWKALKAGGKPVIIGVGGCVASQ
HCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHCCHHHHCCCCCEEEEECCCEECCC
EGEAIVKRAPYVDLVFGPQTLHRLPELIRARRESGKSQVDISFPEIEKFDRLPEPRAEGP
CCCHHHHCCCEEEEEECHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHHCCCCCCCCCH
SAFVSIMEGCSKYCSFCVVPYTRGEEVSRPFEDVLVEVAQLAAQGVREINLLGQNVNAYR
HHHHHHHHHHHHHHCEEEEECCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHC
GAYGADAGDAAQYADLGLLIRTIAQIEGIGRIRFTTSHPLEFSDSLVDAYRDVPQLANYL
CCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHE
HLPVQAGSDRILSAMKRGYTALEFKSKIRKLRAVRPDISISSDFIVGFPGETEADFEKTM
ECCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCHHHHHHHH
KLIEDVGFDQSFSFVYSRRPGTPASDLQDDTPEAVKQARLARLQAHINAHAASISQSMVG
HHHHHCCCCCCCHHEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SVQRVLVEGPSRRDPNELTGKSENMRPVNFPGNPRLIGQFVDVLITEAMSNSLRGRIQLD
HHHHHHHCCCCCCCCHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEC
DSAH
CCCC
>Mature Secondary Structure 
PGTPVSDLSAATAVDATALLPLPVARPSAPAMVRGKLYIKTHGCQMNEYDSAKMADVLA
CCCCCCHHHHHHHHCHHHCCCCCCCCCCCCCEEEEEEEEEECCCCCCCCCCHHHHHHHH
ASEGLELTDDPEEADVVLVNTCSIREKAQEKVFSQLGRWKALKAGGKPVIIGVGGCVASQ
HCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHCCHHHHCCCCCEEEEECCCEECCC
EGEAIVKRAPYVDLVFGPQTLHRLPELIRARRESGKSQVDISFPEIEKFDRLPEPRAEGP
CCCHHHHCCCEEEEEECHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHHCCCCCCCCCH
SAFVSIMEGCSKYCSFCVVPYTRGEEVSRPFEDVLVEVAQLAAQGVREINLLGQNVNAYR
HHHHHHHHHHHHHHCEEEEECCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHC
GAYGADAGDAAQYADLGLLIRTIAQIEGIGRIRFTTSHPLEFSDSLVDAYRDVPQLANYL
CCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHE
HLPVQAGSDRILSAMKRGYTALEFKSKIRKLRAVRPDISISSDFIVGFPGETEADFEKTM
ECCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCHHHHHHHH
KLIEDVGFDQSFSFVYSRRPGTPASDLQDDTPEAVKQARLARLQAHINAHAASISQSMVG
HHHHHCCCCCCCHHEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SVQRVLVEGPSRRDPNELTGKSENMRPVNFPGNPRLIGQFVDVLITEAMSNSLRGRIQLD
HHHHHHHCCCCCCCCHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEC
DSAH
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA