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The map label for this gene is proB [H]

Identifier: 77748647

GI number: 77748647

Start: 2738638

End: 2739795

Strand: Reverse

Name: proB [H]

Synonym: XAC2343

Alternate gene names: 77748647

Gene position: 2739795-2738638 (Counterclockwise)

Preceding gene: 21243078

Following gene: 21243076

Centisome position: 52.94

GC content: 67.36

Gene sequence:

>1158_bases
ATGAGCACGCCTGCCAGCACGTCGTCGCCCTTTACCGAGCAGGCATTGCCGCCATGGCGCCGTGCGGTGCTCAAGGTCGG
CAGCAGCCTGCTCGCCGCCGATGGGGGGGGGCTGTCCCCGCGCTTCGCGCTCGGTCTGGCGCAGTTCGTCTCCGCCAATC
TGGCAGCCGGGCGCGAGCTGGTGATCGTGTCGTCGGGCGCGGTGGCCGCCGGCCGTGCCATCCTGCCCAAAGCGATGGAT
GTCGGCGCACCGATCGCGGCACGGCAGGCACTGGCCGCCTTGGGCCAGGCGCAGTTGATTGCGTTGTGGCAGCGCTTTTT
CGAGCGACCGGTGGCGCAGGTACTGCTCACGCATGACGACCTGCGCAACCGTCGCCGCTACCTCAATGCGCGTGCCACCT
TGGGCGAGTTGCTGCGGCTGGGCGCGCTGCCGGTGATCAACGAAAACGATACCGTCTCGGTGGATGAGTTGAAGCTGGGC
GATAACGACAACCTGGCTGCGATCGTGGCTGCGCTGGTCGATGCCGACGCGTTGTTTATCGCCACCGATATCGATGGCTT
GTATAGCGCCGACCCACGCAGCAACCCGTTGGCGCGCCCGCTCGACGATGTGCCCGAGCTCACTCCGGAGGTGCTGGCGA
TGGCCGGCGGCAGCGGTAGCAATGTGGGTACCGGAGGCATGCGCACCAAACTGGAAGCCGCTGCAAAGGCCGGCGCGGCC
GGCATCGAAACCTATCTGTTCAACGGCCGAAGTGGCGAGGTGGTGCGCGCCCTGGCGCTGGACCGCCTGCGTGGCACGCG
GATCCACGCCGCACGCACGCGCATCGCTGCGCGAAAATACTGGCTGCGGCACGCACCGGTGGAAGCGGGCGCGATCCTCA
TCGACGACGGCGCCGCAGCGGCCTTGACCGGCAAGGGCGCTTCGCTGTTGCCAGGCGGGGTGGCCGGCGCGCAGGGCGAT
TTCCGTCGCGGCGACATGGTGGAGATCCGTCGGCGCGATACCACGGGCGACGGACAGTGTCTGGCACGCGGCGTCAGCCA
GTATTCGGCCCTGGATATCCGTCGTATCGCAGGCCGCCATTCGCGCGAGATCGAGAACGTGCTCGGCTACAGTTACGGCG
AAAACGTGGTGCATCGCGATGATTTGGTGGTGTTGTAG

Upstream 100 bases:

>100_bases
GAAGACGCCCAGCAGATCGTTGCGCGTGACCCGCTGGTGCTGCGCGATGCCTCTGCACTGACCGTGTATGAGTGGAACAC
GCACTGAGGACGTCATTGCC

Downstream 100 bases:

>100_bases
CGGGGAGTGGAGAATCGGGAATGGAGAATCGCAAAAGCTTCGCACCGAGCGTTTCCCAGCGCCGATTCTGTCGCAGTAAA
GTGATGCCGCGTGCTTTTAC

Product: gamma-glutamyl kinase

Products: NA

Alternate protein names: Gamma-glutamyl kinase; GK [H]

Number of amino acids: Translated: 385; Mature: 384

Protein sequence:

>385_residues
MSTPASTSSPFTEQALPPWRRAVLKVGSSLLAADGGGLSPRFALGLAQFVSANLAAGRELVIVSSGAVAAGRAILPKAMD
VGAPIAARQALAALGQAQLIALWQRFFERPVAQVLLTHDDLRNRRRYLNARATLGELLRLGALPVINENDTVSVDELKLG
DNDNLAAIVAALVDADALFIATDIDGLYSADPRSNPLARPLDDVPELTPEVLAMAGGSGSNVGTGGMRTKLEAAAKAGAA
GIETYLFNGRSGEVVRALALDRLRGTRIHAARTRIAARKYWLRHAPVEAGAILIDDGAAAALTGKGASLLPGGVAGAQGD
FRRGDMVEIRRRDTTGDGQCLARGVSQYSALDIRRIAGRHSREIENVLGYSYGENVVHRDDLVVL

Sequences:

>Translated_385_residues
MSTPASTSSPFTEQALPPWRRAVLKVGSSLLAADGGGLSPRFALGLAQFVSANLAAGRELVIVSSGAVAAGRAILPKAMD
VGAPIAARQALAALGQAQLIALWQRFFERPVAQVLLTHDDLRNRRRYLNARATLGELLRLGALPVINENDTVSVDELKLG
DNDNLAAIVAALVDADALFIATDIDGLYSADPRSNPLARPLDDVPELTPEVLAMAGGSGSNVGTGGMRTKLEAAAKAGAA
GIETYLFNGRSGEVVRALALDRLRGTRIHAARTRIAARKYWLRHAPVEAGAILIDDGAAAALTGKGASLLPGGVAGAQGD
FRRGDMVEIRRRDTTGDGQCLARGVSQYSALDIRRIAGRHSREIENVLGYSYGENVVHRDDLVVL
>Mature_384_residues
STPASTSSPFTEQALPPWRRAVLKVGSSLLAADGGGLSPRFALGLAQFVSANLAAGRELVIVSSGAVAAGRAILPKAMDV
GAPIAARQALAALGQAQLIALWQRFFERPVAQVLLTHDDLRNRRRYLNARATLGELLRLGALPVINENDTVSVDELKLGD
NDNLAAIVAALVDADALFIATDIDGLYSADPRSNPLARPLDDVPELTPEVLAMAGGSGSNVGTGGMRTKLEAAAKAGAAG
IETYLFNGRSGEVVRALALDRLRGTRIHAARTRIAARKYWLRHAPVEAGAILIDDGAAAALTGKGASLLPGGVAGAQGDF
RRGDMVEIRRRDTTGDGQCLARGVSQYSALDIRRIAGRHSREIENVLGYSYGENVVHRDDLVVL

Specific function: Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5- oxoproline [H]

COG id: COG0263

COG function: function code E; Glutamate 5-kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Homo sapiens, GI21361368, Length=283, Percent_Identity=29.3286219081272, Blast_Score=104, Evalue=1e-22,
Organism=Homo sapiens, GI62912457, Length=281, Percent_Identity=29.1814946619217, Blast_Score=101, Evalue=1e-21,
Organism=Escherichia coli, GI1786437, Length=368, Percent_Identity=44.8369565217391, Blast_Score=271, Evalue=4e-74,
Organism=Caenorhabditis elegans, GI17569969, Length=268, Percent_Identity=32.4626865671642, Blast_Score=116, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI17569967, Length=256, Percent_Identity=32.8125, Blast_Score=115, Evalue=3e-26,
Organism=Saccharomyces cerevisiae, GI6320506, Length=418, Percent_Identity=36.8421052631579, Blast_Score=237, Evalue=2e-63,
Organism=Saccharomyces cerevisiae, GI6321822, Length=417, Percent_Identity=35.4916067146283, Blast_Score=223, Evalue=4e-59,
Organism=Drosophila melanogaster, GI21357643, Length=293, Percent_Identity=32.7645051194539, Blast_Score=110, Evalue=1e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001048
- InterPro:   IPR001057
- InterPro:   IPR011529
- InterPro:   IPR005715
- InterPro:   IPR019797
- InterPro:   IPR002478
- InterPro:   IPR015947 [H]

Pfam domain/function: PF00696 AA_kinase; PF01472 PUA [H]

EC number: =2.7.2.11 [H]

Molecular weight: Translated: 40503; Mature: 40371

Theoretical pI: Translated: 9.34; Mature: 9.34

Prosite motif: PS50890 PUA ; PS00902 GLUTAMATE_5_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTPASTSSPFTEQALPPWRRAVLKVGSSLLAADGGGLSPRFALGLAQFVSANLAAGREL
CCCCCCCCCCCHHHCCCHHHHHHHHHCCHHEECCCCCCCHHHHHHHHHHHHHHHCCCCEE
VIVSSGAVAAGRAILPKAMDVGAPIAARQALAALGQAQLIALWQRFFERPVAQVLLTHDD
EEEECCCHHCCCHHCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LRNRRRYLNARATLGELLRLGALPVINENDTVSVDELKLGDNDNLAAIVAALVDADALFI
HHHHHHHHHHHHHHHHHHHHCCCCEECCCCCEEHEEEECCCCCCHHHHHHHHHCCCEEEE
ATDIDGLYSADPRSNPLARPLDDVPELTPEVLAMAGGSGSNVGTGGMRTKLEAAAKAGAA
EECCCCCCCCCCCCCCCCCCCHHHHHCCHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCC
GIETYLFNGRSGEVVRALALDRLRGTRIHAARTRIAARKYWLRHAPVEAGAILIDDGAAA
CCEEEEECCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCEE
ALTGKGASLLPGGVAGAQGDFRRGDMVEIRRRDTTGDGQCLARGVSQYSALDIRRIAGRH
EEECCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCH
SREIENVLGYSYGENVVHRDDLVVL
HHHHHHHHCCCCCCCCEECCCEEEC
>Mature Secondary Structure 
STPASTSSPFTEQALPPWRRAVLKVGSSLLAADGGGLSPRFALGLAQFVSANLAAGREL
CCCCCCCCCCHHHCCCHHHHHHHHHCCHHEECCCCCCCHHHHHHHHHHHHHHHCCCCEE
VIVSSGAVAAGRAILPKAMDVGAPIAARQALAALGQAQLIALWQRFFERPVAQVLLTHDD
EEEECCCHHCCCHHCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LRNRRRYLNARATLGELLRLGALPVINENDTVSVDELKLGDNDNLAAIVAALVDADALFI
HHHHHHHHHHHHHHHHHHHHCCCCEECCCCCEEHEEEECCCCCCHHHHHHHHHCCCEEEE
ATDIDGLYSADPRSNPLARPLDDVPELTPEVLAMAGGSGSNVGTGGMRTKLEAAAKAGAA
EECCCCCCCCCCCCCCCCCCCHHHHHCCHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCC
GIETYLFNGRSGEVVRALALDRLRGTRIHAARTRIAARKYWLRHAPVEAGAILIDDGAAA
CCEEEEECCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCEE
ALTGKGASLLPGGVAGAQGDFRRGDMVEIRRRDTTGDGQCLARGVSQYSALDIRRIAGRH
EEECCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCH
SREIENVLGYSYGENVVHRDDLVVL
HHHHHHHHCCCCCCCCEECCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12024217 [H]