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The map label for this gene is proA

Identifier: 21243076

GI number: 21243076

Start: 2737263

End: 2738507

Strand: Reverse

Name: proA

Synonym: XAC2342

Alternate gene names: 21243076

Gene position: 2738507-2737263 (Counterclockwise)

Preceding gene: 77748647

Following gene: 21243075

Centisome position: 52.91

GC content: 66.35

Gene sequence:

>1245_bases
ATGACCATCAAAACGCTCGCTCTGCAATGCCGTGACGCCGCGCAGGTGGTGTCGCAATTGTCTGCACAGGCCAAGCACGC
TTTGCTGGAGGCGATGGCTGCCGCGCTGGAAACCGATGCCGCGGCGATTCTGGCCGCCAATGCGCGCGATCTGGAGGCCG
CGCGTGCCAAGGGCACCGGCAGTGCCATGCTCGATCGGCTGGCGCTGGACGACAAGCGCATGGCCGGTATCGCGGCCGCA
TTGCGCGAAGTGGCGCAGCTGCCCGACCCGGTGGGCCAGATCACCCGCCAAGAGGTGCGCCCCAACGGGATTCGCGTGCA
GAAGATGCGTGTGCCGCTGGGCGTGATCGCGATGATTTACGAGGCGCGGCCCAACGTCACGGCAGACGCTGCCGCGCTGT
GCATCAAGGCCGGCAATGGCGTGATCTTGCGCGGTGGCTCGGAGGCGATCCATTCCAATACCGCCATTGCGCGCGCACTG
CAGGGCGCCTTGCGCGAAGCCAATGTGCCGGAAGCGGCGCTGACCCTGGTCGAGGATCTGCGCCGCGAGACCATGCTGGA
GCTGCTGCAGCTCAACGATATCGTCGATCTGGCGATTCCGCGCGGTGGCGAAGGCCTGATCCGGTTTGTCGCCGAGCACG
CGCGCGTGCCGGTGATCAAGCACTACAAGGGTGTGTGCCATCTGTTTGTGGATGCGTCGGCGGACCTGGCGCTTGCACTA
CGTCTGTTGATCGATGGCAAGGCGACGCGGCCGGCCGCCTGTAATTCGCTGGAGACCTTGCTGGTGCATGCAGACATCGC
CGAGCGCTTTCTCCCGCTTGCGGCGCAGGCATTGCGCGAACGCAAGGTGGAACTGCGTGGCGATGCGGCGACGCGCGCGG
TGCTGCCCGAGATCGCGCCTGCCAGCGACGACGACTATGCCGCGGAATTTCTCGACCTGATCCTGGCCATCCGCGTGGTA
CCGGATCTGGACACGGCGCTGGCGCACATCCGCCAGCACGGCTCGGACCACACCGAAGTCATCGCCACGCAGGATGCCGA
CAACGCCGAGCGCTTCGTGCAGGCGCTACGCTCGGCGGTGGTGATGGTGAACGCCTCCTCGCGGTTTTCCGATGGCGGTG
AACTGGGCCTGGGCGCGGAGATCGGCATCTCCACCACGCGCCTGCATTCGTATGGCCCCATGGGCCTGGAAGCGCTCACC
GTCGAGCGCTTCGTGGTGCGCGGCGAAGGGCAGGTTCGACACTGA

Upstream 100 bases:

>100_bases
GCAAAAGCTTCGCACCGAGCGTTTCCCAGCGCCGATTCTGTCGCAGTAAAGTGATGCCGCGTGCTTTTACCATTCCCGAT
TCCCCACTCCCGATTCTCCA

Downstream 100 bases:

>100_bases
TCGGTAAAGGCGCAACACCACGCTCGCCGGTTTGCGCGCGTTGCATCCATCTGCACCTTCGCCAGCACCACAACAGCAGC
CCTGCAGTGGGGCGGCCTTC

Product: gamma-glutamyl phosphate reductase

Products: NA

Alternate protein names: GPR; Glutamate-5-semialdehyde dehydrogenase; Glutamyl-gamma-semialdehyde dehydrogenase; GSA dehydrogenase

Number of amino acids: Translated: 414; Mature: 413

Protein sequence:

>414_residues
MTIKTLALQCRDAAQVVSQLSAQAKHALLEAMAAALETDAAAILAANARDLEAARAKGTGSAMLDRLALDDKRMAGIAAA
LREVAQLPDPVGQITRQEVRPNGIRVQKMRVPLGVIAMIYEARPNVTADAAALCIKAGNGVILRGGSEAIHSNTAIARAL
QGALREANVPEAALTLVEDLRRETMLELLQLNDIVDLAIPRGGEGLIRFVAEHARVPVIKHYKGVCHLFVDASADLALAL
RLLIDGKATRPAACNSLETLLVHADIAERFLPLAAQALRERKVELRGDAATRAVLPEIAPASDDDYAAEFLDLILAIRVV
PDLDTALAHIRQHGSDHTEVIATQDADNAERFVQALRSAVVMVNASSRFSDGGELGLGAEIGISTTRLHSYGPMGLEALT
VERFVVRGEGQVRH

Sequences:

>Translated_414_residues
MTIKTLALQCRDAAQVVSQLSAQAKHALLEAMAAALETDAAAILAANARDLEAARAKGTGSAMLDRLALDDKRMAGIAAA
LREVAQLPDPVGQITRQEVRPNGIRVQKMRVPLGVIAMIYEARPNVTADAAALCIKAGNGVILRGGSEAIHSNTAIARAL
QGALREANVPEAALTLVEDLRRETMLELLQLNDIVDLAIPRGGEGLIRFVAEHARVPVIKHYKGVCHLFVDASADLALAL
RLLIDGKATRPAACNSLETLLVHADIAERFLPLAAQALRERKVELRGDAATRAVLPEIAPASDDDYAAEFLDLILAIRVV
PDLDTALAHIRQHGSDHTEVIATQDADNAERFVQALRSAVVMVNASSRFSDGGELGLGAEIGISTTRLHSYGPMGLEALT
VERFVVRGEGQVRH
>Mature_413_residues
TIKTLALQCRDAAQVVSQLSAQAKHALLEAMAAALETDAAAILAANARDLEAARAKGTGSAMLDRLALDDKRMAGIAAAL
REVAQLPDPVGQITRQEVRPNGIRVQKMRVPLGVIAMIYEARPNVTADAAALCIKAGNGVILRGGSEAIHSNTAIARALQ
GALREANVPEAALTLVEDLRRETMLELLQLNDIVDLAIPRGGEGLIRFVAEHARVPVIKHYKGVCHLFVDASADLALALR
LLIDGKATRPAACNSLETLLVHADIAERFLPLAAQALRERKVELRGDAATRAVLPEIAPASDDDYAAEFLDLILAIRVVP
DLDTALAHIRQHGSDHTEVIATQDADNAERFVQALRSAVVMVNASSRFSDGGELGLGAEIGISTTRLHSYGPMGLEALTV
ERFVVRGEGQVRH

Specific function: Catalyzes the NADPH dependent reduction of L-gamma- glutamyl 5-phosphate into L-glutamate 5-semialdehyde and phosphate. The product spontaneously undergoes cyclization to form 1-pyrroline-5-carboxylate

COG id: COG0014

COG function: function code E; Gamma-glutamyl phosphate reductase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gamma-glutamyl phosphate reductase family

Homologues:

Organism=Homo sapiens, GI21361368, Length=416, Percent_Identity=37.2596153846154, Blast_Score=265, Evalue=8e-71,
Organism=Homo sapiens, GI62912457, Length=416, Percent_Identity=37.2596153846154, Blast_Score=265, Evalue=8e-71,
Organism=Escherichia coli, GI1786438, Length=415, Percent_Identity=44.578313253012, Blast_Score=362, Evalue=1e-101,
Organism=Caenorhabditis elegans, GI17569969, Length=414, Percent_Identity=35.024154589372, Blast_Score=254, Evalue=6e-68,
Organism=Caenorhabditis elegans, GI17569967, Length=414, Percent_Identity=35.024154589372, Blast_Score=254, Evalue=6e-68,
Organism=Saccharomyces cerevisiae, GI6324899, Length=427, Percent_Identity=32.7868852459016, Blast_Score=259, Evalue=6e-70,
Organism=Drosophila melanogaster, GI21357643, Length=410, Percent_Identity=34.6341463414634, Blast_Score=247, Evalue=8e-66,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PROA_XANAC (Q8PK35)

Other databases:

- EMBL:   AE008923
- RefSeq:   NP_642658.1
- ProteinModelPortal:   Q8PK35
- SMR:   Q8PK35
- GeneID:   1156413
- GenomeReviews:   AE008923_GR
- KEGG:   xac:XAC2342
- NMPDR:   fig|190486.1.peg.2302
- HOGENOM:   HBG318080
- OMA:   GICGAME
- ProtClustDB:   PRK00197
- BioCyc:   XAXO190486:XAC2342-MONOMER
- BRENDA:   1.2.1.41
- GO:   GO:0005737
- HAMAP:   MF_00412
- InterPro:   IPR016161
- InterPro:   IPR016163
- InterPro:   IPR016162
- InterPro:   IPR015590
- InterPro:   IPR000965
- InterPro:   IPR020593
- InterPro:   IPR012134
- Gene3D:   G3DSA:3.40.309.10
- Gene3D:   G3DSA:3.40.605.10
- PANTHER:   PTHR11063:SF1
- PIRSF:   PIRSF000151
- TIGRFAMs:   TIGR00407

Pfam domain/function: PF00171 Aldedh; SSF53720 Aldehyde_DH/Histidinol_DH

EC number: =1.2.1.41

Molecular weight: Translated: 44151; Mature: 44020

Theoretical pI: Translated: 6.15; Mature: 6.15

Prosite motif: PS01223 PROA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIKTLALQCRDAAQVVSQLSAQAKHALLEAMAAALETDAAAILAANARDLEAARAKGTG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHEEECCCHHHHHHHCCCCH
SAMLDRLALDDKRMAGIAAALREVAQLPDPVGQITRQEVRPNGIRVQKMRVPLGVIAMIY
HHHHHHHHCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCEEEEEEECCHHHHHHHH
EARPNVTADAAALCIKAGNGVILRGGSEAIHSNTAIARALQGALREANVPEAALTLVEDL
HCCCCCCHHHHHHEEECCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
RRETMLELLQLNDIVDLAIPRGGEGLIRFVAEHARVPVIKHYKGVCHLFVDASADLALAL
HHHHHHHHHHHCCHHHCCCCCCCHHHHHHHHHHHCCCHHHHCCCEEEEEEECCCHHHHHH
RLLIDGKATRPAACNSLETLLVHADIAERFLPLAAQALRERKVELRGDAATRAVLPEIAP
HHHHCCCCCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCHHHHHHCCCCCC
ASDDDYAAEFLDLILAIRVVPDLDTALAHIRQHGSDHTEVIATQDADNAERFVQALRSAV
CCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHE
VMVNASSRFSDGGELGLGAEIGISTTRLHSYGPMGLEALTVERFVVRGEGQVRH
EEEECCCCCCCCCCCCCCCCCCCEEHEECCCCCCCHHHHHHHHHHEECCCCCCC
>Mature Secondary Structure 
TIKTLALQCRDAAQVVSQLSAQAKHALLEAMAAALETDAAAILAANARDLEAARAKGTG
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHEEECCCHHHHHHHCCCCH
SAMLDRLALDDKRMAGIAAALREVAQLPDPVGQITRQEVRPNGIRVQKMRVPLGVIAMIY
HHHHHHHHCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCEEEEEEECCHHHHHHHH
EARPNVTADAAALCIKAGNGVILRGGSEAIHSNTAIARALQGALREANVPEAALTLVEDL
HCCCCCCHHHHHHEEECCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
RRETMLELLQLNDIVDLAIPRGGEGLIRFVAEHARVPVIKHYKGVCHLFVDASADLALAL
HHHHHHHHHHHCCHHHCCCCCCCHHHHHHHHHHHCCCHHHHCCCEEEEEEECCCHHHHHH
RLLIDGKATRPAACNSLETLLVHADIAERFLPLAAQALRERKVELRGDAATRAVLPEIAP
HHHHCCCCCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCHHHHHHCCCCCC
ASDDDYAAEFLDLILAIRVVPDLDTALAHIRQHGSDHTEVIATQDADNAERFVQALRSAV
CCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHE
VMVNASSRFSDGGELGLGAEIGISTTRLHSYGPMGLEALTVERFVVRGEGQVRH
EEEECCCCCCCCCCCCCCCCCCCEEHEECCCCCCCHHHHHHHHHHEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12024217