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The map label for this gene is nfdA [H]

Identifier: 77748632

GI number: 77748632

Start: 2532952

End: 2534637

Strand: Reverse

Name: nfdA [H]

Synonym: XAC2163

Alternate gene names: 77748632

Gene position: 2534637-2532952 (Counterclockwise)

Preceding gene: 21242899

Following gene: 21242897

Centisome position: 48.97

GC content: 63.58

Gene sequence:

>1686_bases
ATGAGCCGCATGCCAGGCAAGCACGATGCCGGACGGCGCTTGTGGATGCAGGGCGCCGCAGCCGGTCTTGCGTTGGGTGC
ATTGCCGGCTGCTTCGTCTGCATCAGGGAAGTCTTCAATGGTCGATCTCGTCGTTCGCAATGCGCGCATCACCACGCTCG
ATCCGCGCCAGCCCAACGCTACTGCCATCGCCGTTGCCGATGGGCGCATCGTGGCAGTCGGCGACGATGCGCAGATCATG
GCCTTGGCAAAGGGCGCGCGCACCATCGATGCGCAGGGGCGACGTCTGCTGCCCGGCCTCAACGACAGCCACACGCACCT
GATCCGTGGTGGTCTGAATTACAACCTGGAGCTGCGCTGGGATGGCGTGCGCTCGCTGGCCGATGCAATGGCGATGCTGA
AGGCGCAGGTCGATCGCACGCCAGCGCCGCAGTGGGTGCGCGTGGTCGGCGGTTTCACTGCGTATCAATTCGCTGAAAAA
CGCCTGCCCACGCTTGAGGAGATCAACGCCATCGCGCCGGAAACGCCGGTGTTTCTGTTGCATCTGTACGATCGTGCCTT
ACTCAATCGCGCCGCGCTGCGAGCCTGCGGCTACGACAAGACCACTCCGGATCCGCCGGGCGGGCGCATCGAGCGCGACA
AGTTGGGCAACCCCACGGGTCTGCTGCTGGCCAAGCCCAACGCGTTGATCCTTTACGCAAGCCTGGCCAAAGGCCCGCGC
CTGCCGCTGGAATACCAGGCCAACTCCACTCGCCATTTCATGCGCGAGCTCAACCGGCTCGGGGTCACCTCGGTGATCGA
TGCCGGCGGTGGTTTCCAGAACTATCCGGAGGATTACCAGGTCATCGAACAGCTGCACGCGGCCGATCAACTGAGCGTGC
GTATTGCCTATAACCTGTTCACCCAGAACAAGGGCAGGGAGGTCGAAGACTTCCGCGGCTGGACCGAAGTACTGCCGTTA
CGCAAGGGCGATGACCTGCTGCGTCACAACGGCGCCGGCGAGATGCTGGTATTTTCCGCTGCAGACTTCGAACAATTCAG
CGAGCCGCGCCCGGAGCTGCCGGCGGAGCTGGAACCCGAGCTGGAGCAAGTGGTGCGGCTGCTGGTGGAAAAGCGCTGGC
CATTCCGTATCCATGCAACATACGACGAAAGCATCGCGCGCATTCTCGATGTCTACGAAAAGGTCAACGCCGACATCCCG
TTCGATGGCCTGCATTGGTTCATCGACCATGCCGAAACGATTACCCCGCGCAATATCGAGCGCATTCGCGCCTTGAACGG
CGGCATCGCCGTGCAACATCGCATGGCCTACCAGGGCGAGGCGTTCGTGCGGCGCTATGGCGTGCAGGCTGCAAGGCAGA
CGCCGCCAGTGCGACGCATGCTCGCAGCGGGAATTCCGGTCGGCGCAGGCACCGATGCCACCCGCGTGGCCAGCTACAAC
CCGTGGGTGGCGCTGTCGTGGCTGGTCACCGGCCGCACGGTGGGCGGTCTGGCCCTGTATGGCGACGAGAATCTGCTCGA
TCGCGAACAGGCACTGCGGTTGTGGACGCAGGGCAGCGCCTGGTTTTCCGGTGACGAGACCAACAAGGGCACCCTGGCGG
CGGGCAGTTGGCCGACTTCATCGTGTTGTCCGACGATTACTTCCGCATCGACGACGCGGCCATCTCCGACATCACCAGCG
TGCTGA

Upstream 100 bases:

>100_bases
GCGGAATGGAATACCCGCTGATGTGGATGCTGGTGTTGCTGGCACTCGCTGCAGCCGGTGGTGGCGACTGGTCGCTGGAT
CGCCTGCGTAGGCGTGTGGC

Downstream 100 bases:

>100_bases
CCGTCCTGGGCGGGCGTGTCGTGCATGGCGAGGGCGACTTTGCCGCGCTCGCCCCCACGCTGCCGCCTGCCATGCCGGAT
TGGTCGCCGGTGAATCGGTT

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 561; Mature: 560

Protein sequence:

>561_residues
MSRMPGKHDAGRRLWMQGAAAGLALGALPAASSASGKSSMVDLVVRNARITTLDPRQPNATAIAVADGRIVAVGDDAQIM
ALAKGARTIDAQGRRLLPGLNDSHTHLIRGGLNYNLELRWDGVRSLADAMAMLKAQVDRTPAPQWVRVVGGFTAYQFAEK
RLPTLEEINAIAPETPVFLLHLYDRALLNRAALRACGYDKTTPDPPGGRIERDKLGNPTGLLLAKPNALILYASLAKGPR
LPLEYQANSTRHFMRELNRLGVTSVIDAGGGFQNYPEDYQVIEQLHAADQLSVRIAYNLFTQNKGREVEDFRGWTEVLPL
RKGDDLLRHNGAGEMLVFSAADFEQFSEPRPELPAELEPELEQVVRLLVEKRWPFRIHATYDESIARILDVYEKVNADIP
FDGLHWFIDHAETITPRNIERIRALNGGIAVQHRMAYQGEAFVRRYGVQAARQTPPVRRMLAAGIPVGAGTDATRVASYN
PWVALSWLVTGRTVGGLALYGDENLLDREQALRLWTQGSAWFSGDETNKGTLAAGSWPTSSCCPTITSASTTRPSPTSPA
C

Sequences:

>Translated_561_residues
MSRMPGKHDAGRRLWMQGAAAGLALGALPAASSASGKSSMVDLVVRNARITTLDPRQPNATAIAVADGRIVAVGDDAQIM
ALAKGARTIDAQGRRLLPGLNDSHTHLIRGGLNYNLELRWDGVRSLADAMAMLKAQVDRTPAPQWVRVVGGFTAYQFAEK
RLPTLEEINAIAPETPVFLLHLYDRALLNRAALRACGYDKTTPDPPGGRIERDKLGNPTGLLLAKPNALILYASLAKGPR
LPLEYQANSTRHFMRELNRLGVTSVIDAGGGFQNYPEDYQVIEQLHAADQLSVRIAYNLFTQNKGREVEDFRGWTEVLPL
RKGDDLLRHNGAGEMLVFSAADFEQFSEPRPELPAELEPELEQVVRLLVEKRWPFRIHATYDESIARILDVYEKVNADIP
FDGLHWFIDHAETITPRNIERIRALNGGIAVQHRMAYQGEAFVRRYGVQAARQTPPVRRMLAAGIPVGAGTDATRVASYN
PWVALSWLVTGRTVGGLALYGDENLLDREQALRLWTQGSAWFSGDETNKGTLAAGSWPTSSCCPTITSASTTRPSPTSPA
C
>Mature_560_residues
SRMPGKHDAGRRLWMQGAAAGLALGALPAASSASGKSSMVDLVVRNARITTLDPRQPNATAIAVADGRIVAVGDDAQIMA
LAKGARTIDAQGRRLLPGLNDSHTHLIRGGLNYNLELRWDGVRSLADAMAMLKAQVDRTPAPQWVRVVGGFTAYQFAEKR
LPTLEEINAIAPETPVFLLHLYDRALLNRAALRACGYDKTTPDPPGGRIERDKLGNPTGLLLAKPNALILYASLAKGPRL
PLEYQANSTRHFMRELNRLGVTSVIDAGGGFQNYPEDYQVIEQLHAADQLSVRIAYNLFTQNKGREVEDFRGWTEVLPLR
KGDDLLRHNGAGEMLVFSAADFEQFSEPRPELPAELEPELEQVVRLLVEKRWPFRIHATYDESIARILDVYEKVNADIPF
DGLHWFIDHAETITPRNIERIRALNGGIAVQHRMAYQGEAFVRRYGVQAARQTPPVRRMLAAGIPVGAGTDATRVASYNP
WVALSWLVTGRTVGGLALYGDENLLDREQALRLWTQGSAWFSGDETNKGTLAAGSWPTSSCCPTITSASTTRPSPTSPAC

Specific function: Hydrolyzes N-substituted formamides, but not amides. N- benzylformamide is the preferred substrate, while N-butylformamide is hydrolyzed at a much lower rate. Has very low activity towards allylformamide, N-(2-cyclohex-1-enylethyl)formamide and N-(alpha-

COG id: COG1574

COG function: function code R; Predicted metal-dependent hydrolase with the TIM-barrel fold

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013108
- InterPro:   IPR011059 [H]

Pfam domain/function: PF07969 Amidohydro_3 [H]

EC number: =3.5.1.91 [H]

Molecular weight: Translated: 61538; Mature: 61407

Theoretical pI: Translated: 8.30; Mature: 8.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRMPGKHDAGRRLWMQGAAAGLALGALPAASSASGKSSMVDLVVRNARITTLDPRQPNA
CCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHEECCEEEEECCCCCCC
TAIAVADGRIVAVGDDAQIMALAKGARTIDAQGRRLLPGLNDSHTHLIRGGLNYNLELRW
CEEEEECCEEEEECCCCEEEEECCCCCEECCCCCEECCCCCCCCCEEEECCCCEEEEEEE
DGVRSLADAMAMLKAQVDRTPAPQWVRVVGGFTAYQFAEKRLPTLEEINAIAPETPVFLL
CHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCHHHHHHHHHCCCCHHHHHCCCCCCCEEHH
HLYDRALLNRAALRACGYDKTTPDPPGGRIERDKLGNPTGLLLAKPNALILYASLAKGPR
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEHHHCCCCCEEEEECCCEEEEEEECCCCCC
LPLEYQANSTRHFMRELNRLGVTSVIDAGGGFQNYPEDYQVIEQLHAADQLSVRIAYNLF
CCEEECCCCHHHHHHHHHHCCCHHEEECCCCCCCCCHHHHHHHHHHHHHHEEEEEEEEEE
TQNKGREVEDFRGWTEVLPLRKGDDLLRHNGAGEMLVFSAADFEQFSEPRPELPAELEPE
ECCCCCCHHHHCCHHHHCCCCCCCHHHHCCCCCCEEEEECCCHHHHCCCCCCCCCCCCHH
LEQVVRLLVEKRWPFRIHATYDESIARILDVYEKVNADIPFDGLHWFIDHAETITPRNIE
HHHHHHHHHHCCCCEEEEECHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCHHH
RIRALNGGIAVQHRMAYQGEAFVRRYGVQAARQTPPVRRMLAAGIPVGAGTDATRVASYN
HHHHHCCCEEEEEHHHHCHHHHHHHHCCHHHHCCCHHHHHHHCCCCCCCCCCCCEEECCC
PWVALSWLVTGRTVGGLALYGDENLLDREQALRLWTQGSAWFSGDETNKGTLAAGSWPTS
CCEEEEEEEECCCCCCEEEECCCCHHCHHHHHHHHCCCCCEECCCCCCCCEEEECCCCCC
SCCPTITSASTTRPSPTSPAC
CCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SRMPGKHDAGRRLWMQGAAAGLALGALPAASSASGKSSMVDLVVRNARITTLDPRQPNA
CCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHEECCEEEEECCCCCCC
TAIAVADGRIVAVGDDAQIMALAKGARTIDAQGRRLLPGLNDSHTHLIRGGLNYNLELRW
CEEEEECCEEEEECCCCEEEEECCCCCEECCCCCEECCCCCCCCCEEEECCCCEEEEEEE
DGVRSLADAMAMLKAQVDRTPAPQWVRVVGGFTAYQFAEKRLPTLEEINAIAPETPVFLL
CHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCHHHHHHHHHCCCCHHHHHCCCCCCCEEHH
HLYDRALLNRAALRACGYDKTTPDPPGGRIERDKLGNPTGLLLAKPNALILYASLAKGPR
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEHHHCCCCCEEEEECCCEEEEEEECCCCCC
LPLEYQANSTRHFMRELNRLGVTSVIDAGGGFQNYPEDYQVIEQLHAADQLSVRIAYNLF
CCEEECCCCHHHHHHHHHHCCCHHEEECCCCCCCCCHHHHHHHHHHHHHHEEEEEEEEEE
TQNKGREVEDFRGWTEVLPLRKGDDLLRHNGAGEMLVFSAADFEQFSEPRPELPAELEPE
ECCCCCCHHHHCCHHHHCCCCCCCHHHHCCCCCCEEEEECCCHHHHCCCCCCCCCCCCHH
LEQVVRLLVEKRWPFRIHATYDESIARILDVYEKVNADIPFDGLHWFIDHAETITPRNIE
HHHHHHHHHHCCCCEEEEECHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCHHH
RIRALNGGIAVQHRMAYQGEAFVRRYGVQAARQTPPVRRMLAAGIPVGAGTDATRVASYN
HHHHHCCCEEEEEHHHHCHHHHHHHHCCHHHHCCCHHHHHHHCCCCCCCCCCCCEEECCC
PWVALSWLVTGRTVGGLALYGDENLLDREQALRLWTQGSAWFSGDETNKGTLAAGSWPTS
CCEEEEEEEECCCCCCEEEECCCCHHCHHHHHHHHCCCCCEECCCCCCCCEEEECCCCCC
SCCPTITSASTTRPSPTSPAC
CCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA