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The map label for this gene is vsr [H]

Identifier: 77465764

GI number: 77465764

Start: 887183

End: 887611

Strand: Reverse

Name: vsr [H]

Synonym: RSP_3762

Alternate gene names: 77465764

Gene position: 887611-887183 (Counterclockwise)

Preceding gene: 77465765

Following gene: 77465763

Centisome position: 94.12

GC content: 63.17

Gene sequence:

>429_bases
ATGGCAGACACGAGATCTCCGGAGCAACGCCGGCAGATCATGCGGGCGGTGGGGACCAAGAACACGGGCCCCGAGCTCAT
CGTGCGCTCGTTGCTGCACGCAAAAGGCTATCGCTATCGACTCCACGCGAGGTCTCTGCCCGGACAACCGGACATAGTCT
TCCCAGCCCGGCACAAGGCCATCTTCGTGAACGGGTGCTTCTGGCACGGACACGACTGCGCAAAGGGGCGGTTGCCGACC
TCTCGACTGGACTACTGGGGCCCGAAAATCGAGGCGAACGTCGCACGGGATCGCAGGAAGCTCTCCGAGCTCGAGGCGCT
CGGCTGGCAGACGCTGGTCGTCTGGCAGTGCGAACTGAAAGACATCGAGCGCGTGGGATCGCGGTTGTGCGCATTCCTCG
GGCCGACGAAAACCGATCGACAAGCATGA

Upstream 100 bases:

>100_bases
TTGCAGGCCGGTAGCAACCGTCACCTTCACCCGCTCATCGTTGAGCAGATCTTCCGCCGGGCGTACATAGCGCTCAACAC
AGCGAGCAATGGTGAGCCGA

Downstream 100 bases:

>100_bases
TGCGGTAGGCTAATCTGCAGTCCGATCCGCGCCAGCCGAGGAAGCAAACCTTTGAGACCTATTGGAATCGACCTGTTCGC
CGGCGCAGGAGGCCTGTCGC

Product: T/G mismatch-specific endonuclease

Products: DNA [C]

Alternate protein names: V.XorII [H]

Number of amino acids: Translated: 142; Mature: 141

Protein sequence:

>142_residues
MADTRSPEQRRQIMRAVGTKNTGPELIVRSLLHAKGYRYRLHARSLPGQPDIVFPARHKAIFVNGCFWHGHDCAKGRLPT
SRLDYWGPKIEANVARDRRKLSELEALGWQTLVVWQCELKDIERVGSRLCAFLGPTKTDRQA

Sequences:

>Translated_142_residues
MADTRSPEQRRQIMRAVGTKNTGPELIVRSLLHAKGYRYRLHARSLPGQPDIVFPARHKAIFVNGCFWHGHDCAKGRLPT
SRLDYWGPKIEANVARDRRKLSELEALGWQTLVVWQCELKDIERVGSRLCAFLGPTKTDRQA
>Mature_141_residues
ADTRSPEQRRQIMRAVGTKNTGPELIVRSLLHAKGYRYRLHARSLPGQPDIVFPARHKAIFVNGCFWHGHDCAKGRLPTS
RLDYWGPKIEANVARDRRKLSELEALGWQTLVVWQCELKDIERVGSRLCAFLGPTKTDRQA

Specific function: May nick XorII sequences that contain T:G mispairs resulting from m5C-deamination [H]

COG id: COG3727

COG function: function code L; DNA G:T-mismatch repair endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the vsr family [H]

Homologues:

Organism=Escherichia coli, GI1788270, Length=125, Percent_Identity=42.4, Blast_Score=115, Evalue=1e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004603
- InterPro:   IPR011335 [H]

Pfam domain/function: PF03852 Vsr [H]

EC number: 3.1.-.- [C]

Molecular weight: Translated: 16247; Mature: 16116

Theoretical pI: Translated: 10.67; Mature: 10.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADTRSPEQRRQIMRAVGTKNTGPELIVRSLLHAKGYRYRLHARSLPGQPDIVFPARHKA
CCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCEEEEEECCCCCCCCEEECCCCCE
IFVNGCFWHGHDCAKGRLPTSRLDYWGPKIEANVARDRRKLSELEALGWQTLVVWQCELK
EEEECCEECCCCCCCCCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEEEECHH
DIERVGSRLCAFLGPTKTDRQA
HHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
ADTRSPEQRRQIMRAVGTKNTGPELIVRSLLHAKGYRYRLHARSLPGQPDIVFPARHKA
CCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCEEEEEECCCCCCCCEEECCCCCE
IFVNGCFWHGHDCAKGRLPTSRLDYWGPKIEANVARDRRKLSELEALGWQTLVVWQCELK
EEEECCEECCCCCCCCCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEEEECHH
DIERVGSRLCAFLGPTKTDRQA
HHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: DNA mismatch endonuclease vsr specifically recognizes a T-G mismatch in a special context related to the dcm methylation sequence and makes an incision 5' to the mismatched T residue [C]

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8078464 [H]