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The map label for this gene is purR [H]

Identifier: 77465694

GI number: 77465694

Start: 807600

End: 808640

Strand: Direct

Name: purR [H]

Synonym: RSP_3686

Alternate gene names: 77465694

Gene position: 807600-808640 (Clockwise)

Preceding gene: 77465693

Following gene: 77465695

Centisome position: 85.64

GC content: 69.45

Gene sequence:

>1041_bases
ATGGCCAATCTTCAGGATGTGGCGCGGGCGGCGAACGTGTCGAAGACGACCGTTTCGCGCTATCTGAACGGCTCGCTCGA
GCTGCCTGCGCGCACGGCCGAGGCCATCGACACGGCCATCCGCACCCTGAACTACAGCCCCAATCCCCATGCCCGGCGGC
TGAGCCTCGGACGGTCGGATACGATCGCGCTCGTGGTGCCCGACATTGCCACGCCCTTCTTCGCCACCTTCGTGGCGGCG
GTCGAGGCCGAGGCCGATGCGCGGGGCCTGTCGCTCGCGCTGCATGCCACGCTGAACCGCCCCGACCGCGAACTCAAATA
TCTCGATCATATCCGGCAGGGCCATGCCGACGGGCTGATCTTCATCACCAACCATGCGGGCTCGCGGGCACTGGCCGAGA
AGGTCAACAGCGGCGGTCCCTGCGTGGTGGTGGACGAGGACATCGCCGAGGCACGGGTGCCGAAACTCTTCTGCGACAAT
GCCGAGGGCGGGCGGCTTGCGGGCCGCCGGCTCGCCCGCGCGGGCCACCGCCATGTGCTCTTCATCGGCGGCGTCGACCA
GATGATCTCGGGCGCGCGCCGCTATGCGGGCTTCCTCGAGGGGATGCGCGAGATCGCAGGCCCGGACGTGCGCATCGAAC
GCTCCTGCGGGCCCTACACGTTCGAGGCGGGACGCGCCGCGGCGCAGGCCTTCGTCGCCCGCGGGCCGGACCGCCCCACC
GCGATCTTCGCCACCTCGGATGAGCTCACCATCGGGCTCTACGAGGTGCTCCGCGAGGCGGGCCTGTCGATCCCGCGCGA
CGTCTCCGTCGTGGGCTTCGACGATGTGGGCCCGCTCCATCTTTTCGCACCGGCCGTGACAGCGGTGCGGCAACCGGTTC
GGGATCTGGGCAGGCGCGCCCTCGAGCTGCTGCTCGCCCCCCCGCCGGTCGACCCCGAAAGGCTCCCGCCAGAGGAGCTG
CTTCCTGTCACGCTGATCGAGCGGGAGTCCGTTGCACCGCCGGTCGGACAGACCAGACGCAACACAAACAGCGAGGATTG
A

Upstream 100 bases:

>100_bases
AATGGCGCTTTACAGCAGCGGGAGATTCGGCCTAGCGTTCAGATAAACCAGTTTACTAAACCGATTTACTTCGGGATCGG
GATCATCTGGACGAGGTAGA

Downstream 100 bases:

>100_bases
CCATGACCCCCCACTTCACCCGCCGTGCCCTCGGCGCCCTGCTGACCTCGAGCCTGCTCGGCGCCGCGGCCCTGCCCGCC
TTCGCGCAGGAGCAGAAAAC

Product: LacI family transcription regulator

Products: NA

Alternate protein names: Pur regulon repressor; Purine nucleotide synthesis repressor [H]

Number of amino acids: Translated: 346; Mature: 345

Protein sequence:

>346_residues
MANLQDVARAANVSKTTVSRYLNGSLELPARTAEAIDTAIRTLNYSPNPHARRLSLGRSDTIALVVPDIATPFFATFVAA
VEAEADARGLSLALHATLNRPDRELKYLDHIRQGHADGLIFITNHAGSRALAEKVNSGGPCVVVDEDIAEARVPKLFCDN
AEGGRLAGRRLARAGHRHVLFIGGVDQMISGARRYAGFLEGMREIAGPDVRIERSCGPYTFEAGRAAAQAFVARGPDRPT
AIFATSDELTIGLYEVLREAGLSIPRDVSVVGFDDVGPLHLFAPAVTAVRQPVRDLGRRALELLLAPPPVDPERLPPEEL
LPVTLIERESVAPPVGQTRRNTNSED

Sequences:

>Translated_346_residues
MANLQDVARAANVSKTTVSRYLNGSLELPARTAEAIDTAIRTLNYSPNPHARRLSLGRSDTIALVVPDIATPFFATFVAA
VEAEADARGLSLALHATLNRPDRELKYLDHIRQGHADGLIFITNHAGSRALAEKVNSGGPCVVVDEDIAEARVPKLFCDN
AEGGRLAGRRLARAGHRHVLFIGGVDQMISGARRYAGFLEGMREIAGPDVRIERSCGPYTFEAGRAAAQAFVARGPDRPT
AIFATSDELTIGLYEVLREAGLSIPRDVSVVGFDDVGPLHLFAPAVTAVRQPVRDLGRRALELLLAPPPVDPERLPPEEL
LPVTLIERESVAPPVGQTRRNTNSED
>Mature_345_residues
ANLQDVARAANVSKTTVSRYLNGSLELPARTAEAIDTAIRTLNYSPNPHARRLSLGRSDTIALVVPDIATPFFATFVAAV
EAEADARGLSLALHATLNRPDRELKYLDHIRQGHADGLIFITNHAGSRALAEKVNSGGPCVVVDEDIAEARVPKLFCDNA
EGGRLAGRRLARAGHRHVLFIGGVDQMISGARRYAGFLEGMREIAGPDVRIERSCGPYTFEAGRAAAQAFVARGPDRPTA
IFATSDELTIGLYEVLREAGLSIPRDVSVVGFDDVGPLHLFAPAVTAVRQPVRDLGRRALELLLAPPPVDPERLPPEELL
PVTLIERESVAPPVGQTRRNTNSED

Specific function: Is the main repressor of the genes involved in the de novo synthesis of purine nucleotides, regulating purB, purC, purEK, purF, purHD, purL, purMN and guaBA expression. PurR is allosterically activated to bind its cognate DNA by binding the purine corepre

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787948, Length=336, Percent_Identity=35.7142857142857, Blast_Score=169, Evalue=2e-43,
Organism=Escherichia coli, GI1790369, Length=306, Percent_Identity=30.3921568627451, Blast_Score=144, Evalue=6e-36,
Organism=Escherichia coli, GI1790194, Length=336, Percent_Identity=29.7619047619048, Blast_Score=141, Evalue=5e-35,
Organism=Escherichia coli, GI1788474, Length=355, Percent_Identity=30.7042253521127, Blast_Score=137, Evalue=1e-33,
Organism=Escherichia coli, GI1789202, Length=349, Percent_Identity=26.647564469914, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1786540, Length=339, Percent_Identity=27.1386430678466, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI1789068, Length=339, Percent_Identity=24.7787610619469, Blast_Score=105, Evalue=4e-24,
Organism=Escherichia coli, GI1786268, Length=309, Percent_Identity=26.8608414239482, Blast_Score=105, Evalue=6e-24,
Organism=Escherichia coli, GI1787580, Length=311, Percent_Identity=27.0096463022508, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI48994940, Length=313, Percent_Identity=26.517571884984, Blast_Score=85, Evalue=6e-18,
Organism=Escherichia coli, GI1787906, Length=314, Percent_Identity=27.0700636942675, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1790715, Length=307, Percent_Identity=23.1270358306189, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI1790192, Length=225, Percent_Identity=30.2222222222222, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 37292; Mature: 37161

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANLQDVARAANVSKTTVSRYLNGSLELPARTAEAIDTAIRTLNYSPNPHARRLSLGRSD
CCCHHHHHHHHCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHEECCCCC
TIALVVPDIATPFFATFVAAVEAEADARGLSLALHATLNRPDRELKYLDHIRQGHADGLI
EEEEEECCCCHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHCCCCCEE
FITNHAGSRALAEKVNSGGPCVVVDEDIAEARVPKLFCDNAEGGRLAGRRLARAGHRHVL
EEECCCCCHHHHHHHCCCCCEEEECCCHHHHHCCHHEECCCCCCHHHHHHHHHCCCCEEE
FIGGVDQMISGARRYAGFLEGMREIAGPDVRIERSCGPYTFEAGRAAAQAFVARGPDRPT
EECCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCEEEHHHHHHHHHHHHCCCCCCE
AIFATSDELTIGLYEVLREAGLSIPRDVSVVGFDDVGPLHLFAPAVTAVRQPVRDLGRRA
EEEEECCCCHHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHH
LELLLAPPPVDPERLPPEELLPVTLIERESVAPPVGQTRRNTNSED
HHHHCCCCCCCCCCCCHHHCCCEEEECCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
ANLQDVARAANVSKTTVSRYLNGSLELPARTAEAIDTAIRTLNYSPNPHARRLSLGRSD
CCHHHHHHHHCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHEECCCCC
TIALVVPDIATPFFATFVAAVEAEADARGLSLALHATLNRPDRELKYLDHIRQGHADGLI
EEEEEECCCCHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHCCCCCEE
FITNHAGSRALAEKVNSGGPCVVVDEDIAEARVPKLFCDNAEGGRLAGRRLARAGHRHVL
EEECCCCCHHHHHHHCCCCCEEEECCCHHHHHCCHHEECCCCCCHHHHHHHHHCCCCEEE
FIGGVDQMISGARRYAGFLEGMREIAGPDVRIERSCGPYTFEAGRAAAQAFVARGPDRPT
EECCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCEEEHHHHHHHHHHHHCCCCCCE
AIFATSDELTIGLYEVLREAGLSIPRDVSVVGFDDVGPLHLFAPAVTAVRQPVRDLGRRA
EEEEECCCCHHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHH
LELLLAPPPVDPERLPPEELLPVTLIERESVAPPVGQTRRNTNSED
HHHHCCCCCCCCCCCCHHHCCCEEEECCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA