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The map label for this gene is fixK [H]

Identifier: 77465624

GI number: 77465624

Start: 727571

End: 728302

Strand: Reverse

Name: fixK [H]

Synonym: RSP_3616

Alternate gene names: 77465624

Gene position: 728302-727571 (Counterclockwise)

Preceding gene: 77465630

Following gene: 77465622

Centisome position: 77.23

GC content: 64.21

Gene sequence:

>732_bases
ATGCGGGGCCATGACACTCCACTTGTGCGAAAGCTGAATGCCTTCGTCGCATTGTCCGAGACCGAGCTGGAGCTTCTCGG
CAGGATGCACGGCCGCCGCCGCAGTTTCCCGAGGGGGCGCGACATTGTTCACGAGGGACAAGTCAAGCGCTCGGCCTATG
TGCTCGCCTCGGGCTGGGTCTGCTCCTACAAGACCTTGGCAGGGGGCGGCCGGCAGATCGTGGATTTTCAGGTGCCAGGG
GATTTTCTCGGGCTGCGGTCGCTCCTGTTCCGCACCGCCGATCACAACATCGAGCCGGTAACGCGGGTGGAAGCCTCCGA
GGTCTTGATCGCCGACCTTCTTGACGGCTTCGCCCACTCCCCGCGGCTGGCCACCGCCATCCTGTGGGCCGCTTCGCGCG
ACGAAGCAATGGTGGTCGAACATCTGGTCAGCCTCGGGCGCCGGAACGCGCAGGAGCGCACGGCGCATTTCCTTCTGGAA
CTCGGTGCACGCCTGACGCTCGTGGGGATGGGGACGGCGGACGGTTATGCCTGCCCGCTCTCGCAGTATCTGCTTGCGGA
TGCGCTGGGGCTGACCGCTGTCCACATCAACCGCGTCCTGCGCGAGTTGCGCGAGGCGGGCTTGGTGAGCTTCCAGAAAG
GGCAGGTGGAATTCCATGACCTTGCCGGTCTGGTCGCACTCGCCGACTTTGACCTTGCCTATCTGGATCATGCAGGACCG
CTGCTGAAATGA

Upstream 100 bases:

>100_bases
ACGACGATGCTGATCCAGAGCAGCATCCGGGATTCACTGCTGGTTAATTTATCATGATGGCCGGCTTTCGTCCGGAGCCT
GCCAAGGACGAGGGGCGCTG

Downstream 100 bases:

>100_bases
TCCGACCGCCCCACGCTTTGTCGGGAGCGAGGAGGGCGAACAGCTCGACGCTGCCCCGTCCTGACGCTCGCTGGTCTGCC
GCGATGCCGCGAGCCTGCTT

Product: Crp/FNR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 243; Mature: 243

Protein sequence:

>243_residues
MRGHDTPLVRKLNAFVALSETELELLGRMHGRRRSFPRGRDIVHEGQVKRSAYVLASGWVCSYKTLAGGGRQIVDFQVPG
DFLGLRSLLFRTADHNIEPVTRVEASEVLIADLLDGFAHSPRLATAILWAASRDEAMVVEHLVSLGRRNAQERTAHFLLE
LGARLTLVGMGTADGYACPLSQYLLADALGLTAVHINRVLRELREAGLVSFQKGQVEFHDLAGLVALADFDLAYLDHAGP
LLK

Sequences:

>Translated_243_residues
MRGHDTPLVRKLNAFVALSETELELLGRMHGRRRSFPRGRDIVHEGQVKRSAYVLASGWVCSYKTLAGGGRQIVDFQVPG
DFLGLRSLLFRTADHNIEPVTRVEASEVLIADLLDGFAHSPRLATAILWAASRDEAMVVEHLVSLGRRNAQERTAHFLLE
LGARLTLVGMGTADGYACPLSQYLLADALGLTAVHINRVLRELREAGLVSFQKGQVEFHDLAGLVALADFDLAYLDHAGP
LLK
>Mature_243_residues
MRGHDTPLVRKLNAFVALSETELELLGRMHGRRRSFPRGRDIVHEGQVKRSAYVLASGWVCSYKTLAGGGRQIVDFQVPG
DFLGLRSLLFRTADHNIEPVTRVEASEVLIADLLDGFAHSPRLATAILWAASRDEAMVVEHLVSLGRRNAQERTAHFLLE
LGARLTLVGMGTADGYACPLSQYLLADALGLTAVHINRVLRELREAGLVSFQKGQVEFHDLAGLVALADFDLAYLDHAGP
LLK

Specific function: B.japonicum has two fixLJ-dependent fixK homologs that are activators of the transcription of a group of genes involved in anaerobic processes such as denitrification and possibly nitrogen fixation. FixK may bind DNA at the FNR consensus binding site [H]

COG id: COG0664

COG function: function code T; cAMP-binding proteins - catabolite gene activator and regulatory subunit of cAMP-dependent protein kinases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH crp-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787595, Length=223, Percent_Identity=23.3183856502242, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018490
- InterPro:   IPR000595
- InterPro:   IPR001808
- InterPro:   IPR012318
- InterPro:   IPR014710
- InterPro:   IPR018335
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00027 cNMP_binding; PF00325 Crp [H]

EC number: NA

Molecular weight: Translated: 26686; Mature: 26686

Theoretical pI: Translated: 7.37; Mature: 7.37

Prosite motif: PS50042 CNMP_BINDING_3 ; PS51063 HTH_CRP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRGHDTPLVRKLNAFVALSETELELLGRMHGRRRSFPRGRDIVHEGQVKRSAYVLASGWV
CCCCCCHHHHHHHHHHEECHHHHHHHHHHHHHHCCCCCCCCCHHHCCCHHHEEEEECCCE
CSYKTLAGGGRQIVDFQVPGDFLGLRSLLFRTADHNIEPVTRVEASEVLIADLLDGFAHS
EEHHHHCCCCCEEEEEECCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCC
PRLATAILWAASRDEAMVVEHLVSLGRRNAQERTAHFLLELGARLTLVGMGTADGYACPL
CHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCEEEEEECCCCCCCCCCH
SQYLLADALGLTAVHINRVLRELREAGLVSFQKGQVEFHDLAGLVALADFDLAYLDHAGP
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHCCCC
LLK
CCC
>Mature Secondary Structure
MRGHDTPLVRKLNAFVALSETELELLGRMHGRRRSFPRGRDIVHEGQVKRSAYVLASGWV
CCCCCCHHHHHHHHHHEECHHHHHHHHHHHHHHCCCCCCCCCHHHCCCHHHEEEEECCCE
CSYKTLAGGGRQIVDFQVPGDFLGLRSLLFRTADHNIEPVTRVEASEVLIADLLDGFAHS
EEHHHHCCCCCEEEEEECCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCC
PRLATAILWAASRDEAMVVEHLVSLGRRNAQERTAHFLLELGARLTLVGMGTADGYACPL
CHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCEEEEEECCCCCCCCCCH
SQYLLADALGLTAVHINRVLRELREAGLVSFQKGQVEFHDLAGLVALADFDLAYLDHAGP
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHCCCC
LLK
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1551834; 12597275 [H]