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The map label for this gene is miaB [H]

Identifier: 77465601

GI number: 77465601

Start: 692968

End: 694278

Strand: Reverse

Name: miaB [H]

Synonym: RSP_3600

Alternate gene names: 77465601

Gene position: 694278-692968 (Counterclockwise)

Preceding gene: 77465602

Following gene: 77465600

Centisome position: 73.62

GC content: 68.57

Gene sequence:

>1311_bases
ATGTCCGAAGCCCGCAAGCTCTTCATCAAGACCTACGGCTGTCAGATGAATGTCTACGACAGCGAGCGCATGGCCGAGGC
GCTCGGCGCCAAGGGCTATGTGCTGACCGAGGTCGCCGAAGAGGCCGACATGGTGCTCCTGAACACCTGCCACATCCGCG
AGAAGGCGGCGGAGAAGGTCTATTCGGATCTGGGCCGCCTCCGGCCGCTGAAGACCGCCAAGCCCGACCTGAAGATCGGC
GTGGCGGGCTGCGTGGCGCAGGCCGAGGGCGAGGAGATCCTCAAGCGGATGCCGCTCGTGGATCTGGTGGTGGGTCCGCA
GAGCTATCACCGCCTGCCCGACATGCTGGAGCGCACCGAAGGGGGCGCCCGCGTGGTGGACACGGACTTTCCCGAAGAGG
ACAAGTTCGACCATCTGCCCGAGCGCAAGGCCACCCGCGGCCCGGCGGCCTTCCTGACCGTGCAGGAGGGCTGCGACAAG
TTCTGCGCCTTCTGCGTCGTGCCCTACACCCGTGGCGCCGAGGTGAGCCGGCCCTTCGCGCGGCTGATGGCCGAGGCCCG
CGGGCTGGTGGAGCGGGGCGTCCGCGAGATCACGCTTCTGGGGCAGAACGTGAATGCCTGGTCGAACGACGGCCGCGGGC
TCGGCGGGCTCATCCGCGAGCTGGCCCGCATCGACGGGCTCGAGCGGCTGCGCTACACGACCTCGCATCCCAACGACATG
GCCGACGATCTGATCGAGGCCCACGGGGCCGAGCCCAAGCTCATGCCCTATCTGCATCTGCCGGTGCAGTCGGGGTCGGA
CCGGATCCTGAAGGCCATGAACCGCAAGCACACGGCCGAACATTATCTGCGGCTGATCGAGCGGATCCGCGCCGCGCGCC
CCGACATCCTGCTGACCTCGGACTTCATCGTGGGCTTCCCCGGCGAGACCGAAGCCGATTTCGAGGCGACGCTCGATCTG
ATCCGGGCGGTGGGCTTCGGCTCGGCTTTCTCGTTCAAATATTCCGCGCGCCCCGGCACGCCCGCCGCCGAGAAGCCCGA
ACTGCCGGCCGAGGTCTGCGACGCGCGGCTTCAGCGGCTGCAGGCGCTGGTGACCGAGCAGCAGCGGGCGGCACAGATGG
CGATGGTCGGGCGCGAGGTGGGCGTGCTCTACGAGAAGGCCGGGCGCCTGCCCGGACAGATGGTCGGCAAGTCCGACCAT
CTCCACGCCGTCCATGTCGAGGACAAGGCGGGGCAAGTGGGCGATCTGGTGCGCGTGCGCATCACCGCAAGCGCGCCGAA
CTCGCTCGCCGGAGAGCGTCTGGGCGCCTGA

Upstream 100 bases:

>100_bases
GGCCTTGCGGGAGGCCGGTTTCTCGGCTCGGAGCCCATGCCGATTGCATGTGCCCGCCGTTTCCGGTTAAGACCCGCCCT
CATCGAAAGGGGGCGCGCCA

Downstream 100 bases:

>100_bases
TCCCGGCATCGGCCGGAGAGGGGCGCGGCGGCATTCACAACCGCGCTCTTCTCTTGCGTCCTTTCCGTGCAGCGTCACAA
GAAATTGTGGTTTCGTCACC

Product: (dimethylallyl)adenosine tRNA methylthiotransferase

Products: NA

Alternate protein names: tRNA-i(6)A37 methylthiotransferase [H]

Number of amino acids: Translated: 436; Mature: 435

Protein sequence:

>436_residues
MSEARKLFIKTYGCQMNVYDSERMAEALGAKGYVLTEVAEEADMVLLNTCHIREKAAEKVYSDLGRLRPLKTAKPDLKIG
VAGCVAQAEGEEILKRMPLVDLVVGPQSYHRLPDMLERTEGGARVVDTDFPEEDKFDHLPERKATRGPAAFLTVQEGCDK
FCAFCVVPYTRGAEVSRPFARLMAEARGLVERGVREITLLGQNVNAWSNDGRGLGGLIRELARIDGLERLRYTTSHPNDM
ADDLIEAHGAEPKLMPYLHLPVQSGSDRILKAMNRKHTAEHYLRLIERIRAARPDILLTSDFIVGFPGETEADFEATLDL
IRAVGFGSAFSFKYSARPGTPAAEKPELPAEVCDARLQRLQALVTEQQRAAQMAMVGREVGVLYEKAGRLPGQMVGKSDH
LHAVHVEDKAGQVGDLVRVRITASAPNSLAGERLGA

Sequences:

>Translated_436_residues
MSEARKLFIKTYGCQMNVYDSERMAEALGAKGYVLTEVAEEADMVLLNTCHIREKAAEKVYSDLGRLRPLKTAKPDLKIG
VAGCVAQAEGEEILKRMPLVDLVVGPQSYHRLPDMLERTEGGARVVDTDFPEEDKFDHLPERKATRGPAAFLTVQEGCDK
FCAFCVVPYTRGAEVSRPFARLMAEARGLVERGVREITLLGQNVNAWSNDGRGLGGLIRELARIDGLERLRYTTSHPNDM
ADDLIEAHGAEPKLMPYLHLPVQSGSDRILKAMNRKHTAEHYLRLIERIRAARPDILLTSDFIVGFPGETEADFEATLDL
IRAVGFGSAFSFKYSARPGTPAAEKPELPAEVCDARLQRLQALVTEQQRAAQMAMVGREVGVLYEKAGRLPGQMVGKSDH
LHAVHVEDKAGQVGDLVRVRITASAPNSLAGERLGA
>Mature_435_residues
SEARKLFIKTYGCQMNVYDSERMAEALGAKGYVLTEVAEEADMVLLNTCHIREKAAEKVYSDLGRLRPLKTAKPDLKIGV
AGCVAQAEGEEILKRMPLVDLVVGPQSYHRLPDMLERTEGGARVVDTDFPEEDKFDHLPERKATRGPAAFLTVQEGCDKF
CAFCVVPYTRGAEVSRPFARLMAEARGLVERGVREITLLGQNVNAWSNDGRGLGGLIRELARIDGLERLRYTTSHPNDMA
DDLIEAHGAEPKLMPYLHLPVQSGSDRILKAMNRKHTAEHYLRLIERIRAARPDILLTSDFIVGFPGETEADFEATLDLI
RAVGFGSAFSFKYSARPGTPAAEKPELPAEVCDARLQRLQALVTEQQRAAQMAMVGREVGVLYEKAGRLPGQMVGKSDHL
HAVHVEDKAGQVGDLVRVRITASAPNSLAGERLGA

Specific function: Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine [H]

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI28872784, Length=476, Percent_Identity=36.344537815126, Blast_Score=295, Evalue=7e-80,
Organism=Homo sapiens, GI28872782, Length=476, Percent_Identity=36.344537815126, Blast_Score=294, Evalue=1e-79,
Organism=Homo sapiens, GI93277076, Length=446, Percent_Identity=28.6995515695067, Blast_Score=176, Evalue=3e-44,
Organism=Escherichia coli, GI1786882, Length=438, Percent_Identity=51.3698630136986, Blast_Score=431, Evalue=1e-122,
Organism=Escherichia coli, GI1787057, Length=452, Percent_Identity=31.1946902654867, Blast_Score=196, Evalue=3e-51,
Organism=Caenorhabditis elegans, GI17553146, Length=468, Percent_Identity=33.974358974359, Blast_Score=249, Evalue=2e-66,
Organism=Caenorhabditis elegans, GI71996771, Length=370, Percent_Identity=29.1891891891892, Blast_Score=139, Evalue=3e-33,
Organism=Drosophila melanogaster, GI21356207, Length=477, Percent_Identity=34.3815513626834, Blast_Score=273, Evalue=1e-73,
Organism=Drosophila melanogaster, GI19922432, Length=450, Percent_Identity=29.7777777777778, Blast_Score=172, Evalue=4e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006463
- InterPro:   IPR007197
- InterPro:   IPR002792 [H]

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004 [H]

EC number: NA

Molecular weight: Translated: 48000; Mature: 47868

Theoretical pI: Translated: 6.59; Mature: 6.59

Prosite motif: PS50926 TRAM ; PS01278 UPF0004

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEARKLFIKTYGCQMNVYDSERMAEALGAKGYVLTEVAEEADMVLLNTCHIREKAAEKV
CCCHHEEHEEEECCEEECCCHHHHHHHHCCCCEEHHHHHHCCCEEEEHHHHHHHHHHHHH
YSDLGRLRPLKTAKPDLKIGVAGCVAQAEGEEILKRMPLVDLVVGPQSYHRLPDMLERTE
HHHHHHCCCCCCCCCCCEEEHHHHHHHCCHHHHHHHCCHHEEEECCHHHHHHHHHHHHCC
GGARVVDTDFPEEDKFDHLPERKATRGPAAFLTVQEGCDKFCAFCVVPYTRGAEVSRPFA
CCCEEEECCCCCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHCCCCCCCCCCHHHH
RLMAEARGLVERGVREITLLGQNVNAWSNDGRGLGGLIRELARIDGLERLRYTTSHPNDM
HHHHHHHHHHHHCHHEEEEECCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHCCCCCCCHH
ADDLIEAHGAEPKLMPYLHLPVQSGSDRILKAMNRKHTAEHYLRLIERIRAARPDILLTS
HHHHHHHCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEC
DFIVGFPGETEADFEATLDLIRAVGFGSAFSFKYSARPGTPAAEKPELPAEVCDARLQRL
CEEEECCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHH
QALVTEQQRAAQMAMVGREVGVLYEKAGRLPGQMVGKSDHLHAVHVEDKAGQVGDLVRVR
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCEEEEEECCCCCCCCCEEEEE
ITASAPNSLAGERLGA
EEECCCCCCCHHHCCC
>Mature Secondary Structure 
SEARKLFIKTYGCQMNVYDSERMAEALGAKGYVLTEVAEEADMVLLNTCHIREKAAEKV
CCHHEEHEEEECCEEECCCHHHHHHHHCCCCEEHHHHHHCCCEEEEHHHHHHHHHHHHH
YSDLGRLRPLKTAKPDLKIGVAGCVAQAEGEEILKRMPLVDLVVGPQSYHRLPDMLERTE
HHHHHHCCCCCCCCCCCEEEHHHHHHHCCHHHHHHHCCHHEEEECCHHHHHHHHHHHHCC
GGARVVDTDFPEEDKFDHLPERKATRGPAAFLTVQEGCDKFCAFCVVPYTRGAEVSRPFA
CCCEEEECCCCCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHCCCCCCCCCCHHHH
RLMAEARGLVERGVREITLLGQNVNAWSNDGRGLGGLIRELARIDGLERLRYTTSHPNDM
HHHHHHHHHHHHCHHEEEEECCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHCCCCCCCHH
ADDLIEAHGAEPKLMPYLHLPVQSGSDRILKAMNRKHTAEHYLRLIERIRAARPDILLTS
HHHHHHHCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEC
DFIVGFPGETEADFEATLDLIRAVGFGSAFSFKYSARPGTPAAEKPELPAEVCDARLQRL
CEEEECCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHH
QALVTEQQRAAQMAMVGREVGVLYEKAGRLPGQMVGKSDHLHAVHVEDKAGQVGDLVRVR
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCEEEEEECCCCCCCCCEEEEE
ITASAPNSLAGERLGA
EEECCCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA