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The map label for this gene is pcaF [H]

Identifier: 77465535

GI number: 77465535

Start: 619190

End: 620389

Strand: Direct

Name: pcaF [H]

Synonym: RSP_3536

Alternate gene names: 77465535

Gene position: 619190-620389 (Clockwise)

Preceding gene: 77465534

Following gene: 77465536

Centisome position: 65.66

GC content: 72.25

Gene sequence:

>1200_bases
ATGACCCAGGTCTTCATCTGCGACTATATCCGCACGCCCATCGGCCGCTACGGCGGGGCGCTCTCCCCGGTCCGGGCCGA
CGATCTGGGCGCCGTGCCCCTCAAGGCCCTGATGGCCCGCAATGGCGGGGTGGACTGGGAGGCCGTGGACGATGTGATCT
TCGGCTGCGCCAATCAGGCGGGCGAAGACAACCGCAACGTGGCCCGCATGTCGGCGCTGCTGGCGGGGCTGCCGGTGGGT
GTCACGGGCACGACGATCAACCGGCTGTGCGGCTCGGGCATGGATGCCGTGCTGACCGCCGCCCGCCAGATCGCCGCGGG
TGAGGCGGATCTCGTGATCGCGGGCGGGGTGGAGAGCATGTCGCGCGCGCCGTTCGTGCTGCCGAAGGCGGGAACGGCCT
TCACGCGCTCGGCCGAGATCCATGACACGACGATCGGCTGGCGCTTCGTCAATCCGGCGATGGAGGCGGCCTACGGGGTG
GATCCCATGCCGCAGACCGGTCAGAACGTCGCCTGCGACTTCGGCATCAGCCGTGAAGCGCAGGATGCCATGGCCCTCGC
CTCGCAGACGAAGGCGGCGGCGGCGCAGGCGAACGGGCGGCTCGCACAGGAGATCACGCCGGTCCTCGTTCCGCAGCGCC
GGGGCGAGCCGGTCCGGGTGGAGCAGGACGAGCATCCGCGGGCGACGACGCCGGAGGCGCTTGCCAAGCTGAAGCCGCTC
TTCGCCGGCGGATCCGTCACGGCGGGCAATTCCAGCGGCGTCAACGACGGCGCGGCGGCCCTGATCCTCGCCAGCGAGGC
GGCGGTCCGGCGCCACGGGCTTACCCCCATGGCGCGGGTGCTGGGCGGCGCCACCGCGGGCGTGCCTCCGCGCATCATGG
GCATAGGCCCGGTGCCCGCCACCCGCAAGCTGATGGCCCGGCTCGGACTGACCACGGAGGCTTTCGACGTGATCGAGCTG
AACGAGGCCTTCGCCGCGCAGGGGGTGGCCACGCTCCGGCAGCTCGGCATCGCCGAGGAGGATGCGCGGGTGAACCCCAA
CGGCGGCGCCATCGCCCTCGGCCACCCGCTGGGCATGTCGGGCGCCCGCATCACCGGCACGGCGGCGCTCCAGCTGCAGC
GCACGGGCGGGCGGCGGGCACTCTCGACCATGTGCATCGGTGTGGGTCAGGGCATCGCCATCGCGCTGGAACGGGTCTGA

Upstream 100 bases:

>100_bases
GCGCGCAAGACCCGAAGCCGCACGGGCGGAGCATGACCGGCGCGCTAGAGGCCGGCCGGAGCACATCCACGCAAGGAGGG
GCCGATTCGGCCGCACCGAC

Downstream 100 bases:

>100_bases
GATCGGCCACCGGCGGACCTGCTGCGCGGCCTCTCCTGCCGGCTCGGACGACCGAAGCGCAGATCGTCCAGGCTGCCGCC
CCCGCGCAGGAGCGGGACAG

Product: beta-ketoadipyl CoA thiolase

Products: NA

Alternate protein names: 3-oxoadipyl-CoA thiolase [H]

Number of amino acids: Translated: 399; Mature: 398

Protein sequence:

>399_residues
MTQVFICDYIRTPIGRYGGALSPVRADDLGAVPLKALMARNGGVDWEAVDDVIFGCANQAGEDNRNVARMSALLAGLPVG
VTGTTINRLCGSGMDAVLTAARQIAAGEADLVIAGGVESMSRAPFVLPKAGTAFTRSAEIHDTTIGWRFVNPAMEAAYGV
DPMPQTGQNVACDFGISREAQDAMALASQTKAAAAQANGRLAQEITPVLVPQRRGEPVRVEQDEHPRATTPEALAKLKPL
FAGGSVTAGNSSGVNDGAAALILASEAAVRRHGLTPMARVLGGATAGVPPRIMGIGPVPATRKLMARLGLTTEAFDVIEL
NEAFAAQGVATLRQLGIAEEDARVNPNGGAIALGHPLGMSGARITGTAALQLQRTGGRRALSTMCIGVGQGIAIALERV

Sequences:

>Translated_399_residues
MTQVFICDYIRTPIGRYGGALSPVRADDLGAVPLKALMARNGGVDWEAVDDVIFGCANQAGEDNRNVARMSALLAGLPVG
VTGTTINRLCGSGMDAVLTAARQIAAGEADLVIAGGVESMSRAPFVLPKAGTAFTRSAEIHDTTIGWRFVNPAMEAAYGV
DPMPQTGQNVACDFGISREAQDAMALASQTKAAAAQANGRLAQEITPVLVPQRRGEPVRVEQDEHPRATTPEALAKLKPL
FAGGSVTAGNSSGVNDGAAALILASEAAVRRHGLTPMARVLGGATAGVPPRIMGIGPVPATRKLMARLGLTTEAFDVIEL
NEAFAAQGVATLRQLGIAEEDARVNPNGGAIALGHPLGMSGARITGTAALQLQRTGGRRALSTMCIGVGQGIAIALERV
>Mature_398_residues
TQVFICDYIRTPIGRYGGALSPVRADDLGAVPLKALMARNGGVDWEAVDDVIFGCANQAGEDNRNVARMSALLAGLPVGV
TGTTINRLCGSGMDAVLTAARQIAAGEADLVIAGGVESMSRAPFVLPKAGTAFTRSAEIHDTTIGWRFVNPAMEAAYGVD
PMPQTGQNVACDFGISREAQDAMALASQTKAAAAQANGRLAQEITPVLVPQRRGEPVRVEQDEHPRATTPEALAKLKPLF
AGGSVTAGNSSGVNDGAAALILASEAAVRRHGLTPMARVLGGATAGVPPRIMGIGPVPATRKLMARLGLTTEAFDVIELN
EAFAAQGVATLRQLGIAEEDARVNPNGGAIALGHPLGMSGARITGTAALQLQRTGGRRALSTMCIGVGQGIAIALERV

Specific function: Catalyzes thiolytic cleavage of beta-ketoadipyl-CoA to succinyl-CoA and acetyl-CoA [H]

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI167614485, Length=398, Percent_Identity=42.2110552763819, Blast_Score=282, Evalue=5e-76,
Organism=Homo sapiens, GI148539872, Length=400, Percent_Identity=40.5, Blast_Score=267, Evalue=1e-71,
Organism=Homo sapiens, GI4557237, Length=401, Percent_Identity=35.1620947630923, Blast_Score=224, Evalue=9e-59,
Organism=Homo sapiens, GI4501853, Length=395, Percent_Identity=41.0126582278481, Blast_Score=208, Evalue=7e-54,
Organism=Homo sapiens, GI4504327, Length=427, Percent_Identity=31.615925058548, Blast_Score=163, Evalue=3e-40,
Organism=Homo sapiens, GI194353979, Length=394, Percent_Identity=30.4568527918782, Blast_Score=115, Evalue=1e-25,
Organism=Escherichia coli, GI1787663, Length=401, Percent_Identity=63.8403990024938, Blast_Score=500, Evalue=1e-143,
Organism=Escherichia coli, GI87082165, Length=400, Percent_Identity=45.5, Blast_Score=291, Evalue=4e-80,
Organism=Escherichia coli, GI1788554, Length=403, Percent_Identity=45.9057071960298, Blast_Score=283, Evalue=1e-77,
Organism=Escherichia coli, GI48994986, Length=406, Percent_Identity=43.3497536945813, Blast_Score=276, Evalue=2e-75,
Organism=Escherichia coli, GI1788683, Length=427, Percent_Identity=32.0843091334895, Blast_Score=151, Evalue=6e-38,
Organism=Caenorhabditis elegans, GI133906874, Length=399, Percent_Identity=43.3583959899749, Blast_Score=295, Evalue=3e-80,
Organism=Caenorhabditis elegans, GI17535921, Length=400, Percent_Identity=36.25, Blast_Score=222, Evalue=3e-58,
Organism=Caenorhabditis elegans, GI25147385, Length=400, Percent_Identity=34, Blast_Score=213, Evalue=2e-55,
Organism=Caenorhabditis elegans, GI17551802, Length=427, Percent_Identity=29.2740046838407, Blast_Score=162, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI17535917, Length=400, Percent_Identity=30.5, Blast_Score=158, Evalue=4e-39,
Organism=Saccharomyces cerevisiae, GI6325229, Length=407, Percent_Identity=35.8722358722359, Blast_Score=221, Evalue=2e-58,
Organism=Saccharomyces cerevisiae, GI6322031, Length=390, Percent_Identity=35.1282051282051, Blast_Score=188, Evalue=1e-48,
Organism=Drosophila melanogaster, GI24655093, Length=406, Percent_Identity=44.3349753694581, Blast_Score=306, Evalue=1e-83,
Organism=Drosophila melanogaster, GI17648125, Length=401, Percent_Identity=39.9002493765586, Blast_Score=251, Evalue=9e-67,
Organism=Drosophila melanogaster, GI24640423, Length=401, Percent_Identity=34.4139650872818, Blast_Score=218, Evalue=5e-57,
Organism=Drosophila melanogaster, GI17137578, Length=429, Percent_Identity=31.4685314685315, Blast_Score=163, Evalue=2e-40,
Organism=Drosophila melanogaster, GI24585051, Length=364, Percent_Identity=26.9230769230769, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI19921506, Length=363, Percent_Identity=25.6198347107438, Blast_Score=74, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012793
- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: =2.3.1.174 [H]

Molecular weight: Translated: 41042; Mature: 40910

Theoretical pI: Translated: 7.42; Mature: 7.42

Prosite motif: PS00098 THIOLASE_1 ; PS00737 THIOLASE_2 ; PS00099 THIOLASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQVFICDYIRTPIGRYGGALSPVRADDLGAVPLKALMARNGGVDWEAVDDVIFGCANQA
CCEEEEHHHHHCCHHHCCCCCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHCCCC
GEDNRNVARMSALLAGLPVGVTGTTINRLCGSGMDAVLTAARQIAAGEADLVIAGGVESM
CCCCCHHHHHHHHHHCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHCCCCCEEEECCHHHH
SRAPFVLPKAGTAFTRSAEIHDTTIGWRFVNPAMEAAYGVDPMPQTGQNVACDFGISREA
CCCCEEECCCCCCEECCCCCEECCCCEEEECHHHHHHCCCCCCCCCCCCEEEECCCCCHH
QDAMALASQTKAAAAQANGRLAQEITPVLVPQRRGEPVRVEQDEHPRATTPEALAKLKPL
HHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCCCCEEECCCCCCCCCCHHHHHHHHHH
FAGGSVTAGNSSGVNDGAAALILASEAAVRRHGLTPMARVLGGATAGVPPRIMGIGPVPA
HCCCCEECCCCCCCCCCCEEEEEEHHHHHHHCCCCHHHHHHCCCCCCCCCCEEECCCCCH
TRKLMARLGLTTEAFDVIELNEAFAAQGVATLRQLGIAEEDARVNPNGGAIALGHPLGMS
HHHHHHHHCCCHHHHHHEEHHHHHHHHHHHHHHHHCCCCCCCEECCCCCEEEECCCCCCC
GARITGTAALQLQRTGGRRALSTMCIGVGQGIAIALERV
CCEEEEEEEEEEECCCCHHHHHHHHHHCCCCEEEEEECC
>Mature Secondary Structure 
TQVFICDYIRTPIGRYGGALSPVRADDLGAVPLKALMARNGGVDWEAVDDVIFGCANQA
CEEEEHHHHHCCHHHCCCCCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHCCCC
GEDNRNVARMSALLAGLPVGVTGTTINRLCGSGMDAVLTAARQIAAGEADLVIAGGVESM
CCCCCHHHHHHHHHHCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHCCCCCEEEECCHHHH
SRAPFVLPKAGTAFTRSAEIHDTTIGWRFVNPAMEAAYGVDPMPQTGQNVACDFGISREA
CCCCEEECCCCCCEECCCCCEECCCCEEEECHHHHHHCCCCCCCCCCCCEEEECCCCCHH
QDAMALASQTKAAAAQANGRLAQEITPVLVPQRRGEPVRVEQDEHPRATTPEALAKLKPL
HHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCCCCEEECCCCCCCCCCHHHHHHHHHH
FAGGSVTAGNSSGVNDGAAALILASEAAVRRHGLTPMARVLGGATAGVPPRIMGIGPVPA
HCCCCEECCCCCCCCCCCEEEEEEHHHHHHHCCCCHHHHHHCCCCCCCCCCEEECCCCCH
TRKLMARLGLTTEAFDVIELNEAFAAQGVATLRQLGIAEEDARVNPNGGAIALGHPLGMS
HHHHHHHHCCCHHHHHHEEHHHHHHHHHHHHHHHHCCCCCCCEECCCCCEEEECCCCCCC
GARITGTAALQLQRTGGRRALSTMCIGVGQGIAIALERV
CCEEEEEEEEEEECCCCHHHHHHHHHHCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]