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The map label for this gene is potB [H]

Identifier: 77465518

GI number: 77465518

Start: 596537

End: 597385

Strand: Direct

Name: potB [H]

Synonym: RSP_3517

Alternate gene names: 77465518

Gene position: 596537-597385 (Clockwise)

Preceding gene: 77465517

Following gene: 77465519

Centisome position: 63.26

GC content: 67.73

Gene sequence:

>849_bases
ATGGCGTCCGCCCGCCTCACCCTCTTCTGGCTGCTGGCGCTGCCGGTGCTGGTCTTCACCGGCTTCTTCCTGATCCCGCT
CGGCGTGGTGCTCGTCTCGTCCTTCACCGATCCCGAGGCCGGGCTCACCCTTGCCCATTATGCGCGGATCCTGCTCGACG
CCTACCACTGGCAGGTGATCGCCACGACCTTCCGCATCGCGATCTTCTCGACGCTGATCTGCGTGGCCCTCGGCTATCCG
CTGGCCTGGTATCTGGTGCGGATCGTGCAGGTGCGGAGCTGGCGGCGCGCCTGCGTCATCCTCCTCGTCCTGCCGCTCTT
CACCTCGAACATCGTCCGCTCCTTCGGCTGGATGGTGCTTCTGGGCCGCAGCGGGCTCGTGAACGACGCGCTTCTGTCGC
TGGGCCTGATCGACCGGCCGGTGCGCTTTCTGGGCACCGAGACCGGCATCCTGATCGGCATGGTCTATATCCTGCTGCCC
TTCACGGTGCTGACCATCGGCACGGCGCTGGCCCGCGTCGACCGCGGGCTCGAGCAGGCCGCGCGCGATCTGGGCGCCAC
GCCCGCCCGCGCCTTCTGGACCGTGACCTTTCCCCTGACCCTGCACGGGGTGATGGCCGGCGCGATCATCGTCTTCTCGA
TGGCGATCAGCGCCTATGTGACGCCCGCGCTGCTCTCGGGGGGCAAGGTCACGGTGCTCGCGATGCTGGCCTTCCAGCAA
TATTCGACGGTCTTCGATTTCCACTATGGCGGGGCGCTCTCGATCACGCTCCTCGTGCTGACGCTGGCGCTGATCGGACT
GGCCTCGCGGATGACGCGCGGCAGGACGCCCGGCGCGGGAGGACACTGA

Upstream 100 bases:

>100_bases
GCGCGCAGCTACGGTCTGCCGCCCGCGGGGATCTCGGCCGGACGGACGGTGCAGGCGGGCTTCGATCCGGCCAACCTGCA
TCCGCTGGGAGCCTGAACCC

Downstream 100 bases:

>100_bases
CATGGCCGCGCGTCTCGCCGTCCGGGCGCTTGCCCTCCTTACCCTCGCCTATCTGGCGCTGCCGCTCGTCGTGGTGATCG
GCGGCTCGCTGACCGAGACC

Product: ABC polyamine/opine transporter, inner membrane subunit

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 282; Mature: 281

Protein sequence:

>282_residues
MASARLTLFWLLALPVLVFTGFFLIPLGVVLVSSFTDPEAGLTLAHYARILLDAYHWQVIATTFRIAIFSTLICVALGYP
LAWYLVRIVQVRSWRRACVILLVLPLFTSNIVRSFGWMVLLGRSGLVNDALLSLGLIDRPVRFLGTETGILIGMVYILLP
FTVLTIGTALARVDRGLEQAARDLGATPARAFWTVTFPLTLHGVMAGAIIVFSMAISAYVTPALLSGGKVTVLAMLAFQQ
YSTVFDFHYGGALSITLLVLTLALIGLASRMTRGRTPGAGGH

Sequences:

>Translated_282_residues
MASARLTLFWLLALPVLVFTGFFLIPLGVVLVSSFTDPEAGLTLAHYARILLDAYHWQVIATTFRIAIFSTLICVALGYP
LAWYLVRIVQVRSWRRACVILLVLPLFTSNIVRSFGWMVLLGRSGLVNDALLSLGLIDRPVRFLGTETGILIGMVYILLP
FTVLTIGTALARVDRGLEQAARDLGATPARAFWTVTFPLTLHGVMAGAIIVFSMAISAYVTPALLSGGKVTVLAMLAFQQ
YSTVFDFHYGGALSITLLVLTLALIGLASRMTRGRTPGAGGH
>Mature_281_residues
ASARLTLFWLLALPVLVFTGFFLIPLGVVLVSSFTDPEAGLTLAHYARILLDAYHWQVIATTFRIAIFSTLICVALGYPL
AWYLVRIVQVRSWRRACVILLVLPLFTSNIVRSFGWMVLLGRSGLVNDALLSLGLIDRPVRFLGTETGILIGMVYILLPF
TVLTIGTALARVDRGLEQAARDLGATPARAFWTVTFPLTLHGVMAGAIIVFSMAISAYVTPALLSGGKVTVLAMLAFQQY
STVFDFHYGGALSITLLVLTLALIGLASRMTRGRTPGAGGH

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine [H]

COG id: COG1176

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component I

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787369, Length=236, Percent_Identity=34.7457627118644, Blast_Score=148, Evalue=3e-37,
Organism=Escherichia coli, GI1787080, Length=219, Percent_Identity=36.5296803652968, Blast_Score=145, Evalue=3e-36,
Organism=Escherichia coli, GI1787713, Length=245, Percent_Identity=28.9795918367347, Blast_Score=96, Evalue=3e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 30557; Mature: 30426

Theoretical pI: Translated: 10.46; Mature: 10.46

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASARLTLFWLLALPVLVFTGFFLIPLGVVLVSSFTDPEAGLTLAHYARILLDAYHWQVI
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHH
ATTFRIAIFSTLICVALGYPLAWYLVRIVQVRSWRRACVILLVLPLFTSNIVRSFGWMVL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEE
LGRSGLVNDALLSLGLIDRPVRFLGTETGILIGMVYILLPFTVLTIGTALARVDRGLEQA
ECCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ARDLGATPARAFWTVTFPLTLHGVMAGAIIVFSMAISAYVTPALLSGGKVTVLAMLAFQQ
HHHHCCCCCHHEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
YSTVFDFHYGGALSITLLVLTLALIGLASRMTRGRTPGAGGH
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
ASARLTLFWLLALPVLVFTGFFLIPLGVVLVSSFTDPEAGLTLAHYARILLDAYHWQVI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHH
ATTFRIAIFSTLICVALGYPLAWYLVRIVQVRSWRRACVILLVLPLFTSNIVRSFGWMVL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEE
LGRSGLVNDALLSLGLIDRPVRFLGTETGILIGMVYILLPFTVLTIGTALARVDRGLEQA
ECCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ARDLGATPARAFWTVTFPLTLHGVMAGAIIVFSMAISAYVTPALLSGGKVTVLAMLAFQQ
HHHHCCCCCHHEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
YSTVFDFHYGGALSITLLVLTLALIGLASRMTRGRTPGAGGH
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]